中华口腔医学杂志最新文献

{"title":"[Build high-level academic journal platform for stomatology].","authors":"S L Wang","doi":"10.3760/cma.j.cn112144-20241221-00501","DOIUrl":"10.3760/cma.j.cn112144-20241221-00501","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of difficulties in diagnosis and treatment of cracked tooth and research progress].","authors":"F Lin, L Yue","doi":"10.3760/cma.j.cn112144-20241031-00411","DOIUrl":"10.3760/cma.j.cn112144-20241031-00411","url":null,"abstract":"<p><p>The occurrence of cracked tooth is closely related to the abnormal occlusal force. The cracks existing on hard tissue of tooth cannot be self-limiting. As long as the external force exists, the cracks would continue to expand, involving the pulp, periapical, and periodontal tissues, ultimately leading to splitting and tooth loss. Due to the lack of objective indicators to identify cracks, the clinical diagnosis is difficult; due to the uncontrollability of crack propagation and individual differences, the treatment prognosis is uncertain, thus, clinicians are usually confused in choosing the treatment approaches, making it difficult to diagnose and treat this disease. On the other hand, there are many terms in Chinese and English for cracked teeth, which are difficult to correspond with each other. Moreover, the research approaches and methodologies are limited, which slows down the progress of studies in pathogenesis and leads to few breakthroughs in the field of clinical research. By tracing the changes in the terminology of cracked teeth, sorting out the research progress in the literature, and analyzing the difficulties in clinical diagnosis and treatment, the paper proposes the principles and strategies for diagnosing and treating cracked teeth, to assist clinicians in figuring out the structures and clarifying the misunderstandings of this disease.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"8-16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of risk factors for delirium in elderly patients with head and neck cancer after free flap reconstruction surgery].","authors":"S X Chen, F Y Qin, X M Yu, Y J Huang, S N Zhou, W P Gu, Q M Chen","doi":"10.3760/cma.j.cn112144-20240907-00339","DOIUrl":"10.3760/cma.j.cn112144-20240907-00339","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the risk factors for delirium after free flap reconstruction surgery in elderly patients with head and neck cancer. <b>Methods:</b> This study retrospectively collected clinical data from 309 elderly patients with head and neck cancer who underwent radical resection and free flap reconstruction surgery in the Department of Operation & Anesthesiology, College & Hospital of Stomatology, Guangxi Medical University from January 2018 to December 2022. Among them, there were 197 males (63.8%) and 112 females (36.2%), with an average age of (66.8±5.5) years old. Based on the delirium diagnostic criteria outlined in the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders, patients were evaluated for postoperative delirium within one week after surgery. They were then divided into a delirium group (<i>n</i>=75) and a non-delirium group (<i>n</i>=234). Perioperative indicators potentially related to postoperative delirium, including age, gender, past medical history, tumor characteristics, hematological tests, perioperative sleep disturbances, surgical procedures, intraoperative fluid intake and output, anesthetic dosage, postoperative pain, etc, were collected from the relevant medical record system.Variables with <i>P</i><0.05 were included in a multivariate Logistic regression model to screen for independent risk factors for delirium. <b>Results:</b> The incidence of delirium after free flap reconstruction surgery for head and neck cancer in elderly patients was 24.3% (75/309). Multivariate Logistic regression analysis showed that male gender (<i>OR</i>=2.802, <i>P</i>=0.005), perioperative sleep disturbances (<i>OR</i>=7.104, <i>P</i><0.001), and moderate-to-severe postoperative pain (<i>OR</i>=6.903, <i>P</i><0.001) were risk factors for postoperative delirium. <b>Conclusions:</b> Male gender, perioperative sleep disturbances, and moderate-to-severe postoperative pain are independent risk factors for delirium in these patients.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Guidelines for computer-aided design/computer-aided manufacturing custom fiber post-and-core].","authors":"","doi":"10.3760/cma.j.cn112144-20241016-00387","DOIUrl":"10.3760/cma.j.cn112144-20241016-00387","url":null,"abstract":"<p><p>The post-and-core is a widely accepted method to restore endodontically treated teeth with compromised tooth structure. Currently, cast metal post-and-core systems and prefabricated fiber posts combined with composite resin cores are the most frequently options in dental clinical practice, but both also come with advantages and limitations. The development of computer-aided design/computer-aided manufacturing (CAD/CAM) custom fiber post-and-core represents one of the significant trends in the advancement of dentistry. Society of Digital Dental Industry, National Association of Health Industry and Enterprise Management organized experts to formulate Guidelines for CAD/CAM custom fiber post-and-core, standardizing the clinical operation procedures of this technology, enhancing the success rate of CAD/CAM custom fiber post-and-core, and promoting its application and development.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"3-7"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mitochondrial transfer contributes to the odontogenic differentiation of dental mesenchymal stem cells].","authors":"X Y Li, L Zhang, Y Sun","doi":"10.3760/cma.j.cn112144-20240926-00360","DOIUrl":"10.3760/cma.j.cn112144-20240926-00360","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate whether there is mitochondrial transfer in dental mesenchymal stem cells (MSCs) and its significance for the odontogenic differentiation. &lt;b&gt;Methods:&lt;/b&gt; Flow cytometry and immunohistochemical staining were used to isolate dental mesenchymal stem cells. Immunofluorescence staining and live cell imaging were applied to determine whether there is mitochondrial transfer in dental MSCs. Transcriptome sequencing data re-analysis of human dental pulp stem cells (DPSCs) and bone marrow mesenchymal stem cells (BMSCs) from gene expression omnibus (GEO) data base demonstrated the importance of mitochondrial transfer in dental MSCs. Cells were managed with mitochondrial transfer inhibitor ML141 with dimethyl sulfoxide as the control. Immunofluorescence staining, senescence-associated β-galactosidase (SA-β-gal) staining, reactive oxygen species (ROS) assay, 5-ethynyl-2'-deoxyuridine(EdU) labelling, cell counting kit-8 (CCK-8) assay, Western blotting, live cell imaging and transmission electron microscope were used to investigate cell morphology, ROS level, cellular senescence, cell proliferation, MSCs marker paired related homeobox 1 (Prrx1) and Sp7 transcription factor (Sp7) expression, mitochondrial transfer and mitochondrial morphology, respectively. Further, after using ML141 during the induction of odontogenic differentiation, alkaline phosphatase (ALP) chromogenic kit was used to detect ALP activity and real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expression of odontogenic differentiation-related genes Alp, Sp7, dentin matrix protein 1 (Dmp1), and dentin salivary phosphoprotein (Dspp), which were applied to investigate the effect of mitochondrial transfer on odontogenic differentiation. &lt;b&gt;Results:&lt;/b&gt; An ultrafine tunneling nanotubes (TNTs) structure labelled with F-actin existed between dental MSCs, and the presence of transferring mitochondria in this structure was also confirmed. Transcriptome sequencing data suggested that the gene expression profiles were significantly different between DPSCs and BMSCs. Genes related to mitochondrial transfer and mitochondrial dynamic were significantly increased in DPSCs compared to BMSCs. Compared with the control group, treatment with 1, 5, 10 μmol/L ML141, the mitochondrial transfer inhibitor, had little significant effects on the cell morphology, cytoskeleton and ROS level. SA-β-gal activity and the proportion of SA-β-gal positive cells in the ML141-treated groups [(3.93±0.21)%, (3.23±0.42)%, (4.06±0.84)%] had no significant differences with the control group [(3.83±0.28)%] (all &lt;i&gt;P&lt;/i&gt;&gt;0.05). In the cell proliferation assay, the proportion of EdU positive cells in the ML141-treated groups [(20.00±3.82)%, (19.48±1.96)%, (12.55±2.86)%] had no significant differences (all &lt;i&gt;P&lt;/i&gt;&gt;0.05) with the control group [(18.57±0.87)%], whereas the CCK-8 assay showed similar results in ML141-treated group of 1, 5 μmol/L (all &lt;i&gt;P&lt;/i&gt;&gt;0.05). Western blott","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"43-53"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[WWP1 plays a positive role in ameloblast differentiation and enamel formation in mice].","authors":"J X Lin, J X Niu, J Fu, H Feng, Y Liu, G H Yuan, Z Chen","doi":"10.3760/cma.j.cn112144-20240916-00349","DOIUrl":"10.3760/cma.j.cn112144-20240916-00349","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the role of WW domain containing E3 ubiquitin protein ligase 1 (WWP1) in enamel development of mice. <b>Methods:</b> Single-cell RNA sequencing data of incisor tissues of postnatal day 7 (P7) mice and mandibular first molar tooth germs of P3.5 mice were used to analyze the expression of Wwp1 in dental epithelial cells. Immunohistochemistry was performed to observe the distribution and expression levels of WWP1 in the epithelium of mouse incisors and mandibular first molar tooth germs. Wwp1 knockout (Wwp1 KO) mice were generated and collected with their control littermates at P1, P7, three mice per group, as well as at P14, P28, 2 months (2M), and 3M, six mice per group. The enamel volumes of molars and incisors were analyzed using micro-CT. Scanning electron microscopy was employed to examine the enamel cross-sections of Wwp1 KO and control mice. Energy dispersive spectroscopy (EDS) was used to analyze the calcium and phosphorus content of the enamel rod of incisors. Immunofluorescence was performed to detect the expression of amelogenin (AMELX) in the ameloblasts of Wwp1 KO and control mice. Additionally, LS-8 ameloblast-like epithelial cells were cultured, and Wwp1 siRNA or overexpression plasmids were transfected to knock down or overexpress WWP1. The protein levels of AMELX were then assessed by Western blotting. <b>Results:</b> Single-cell sequencing result showed a high Wwp1 mRNA expression level in the epithelial cells of mouse incisors and mandibular molar tooth germs. Immunohistochemistry revealed the expression of WWP1 in presecretory, secretory, transitional, and mature ameloblasts. Wwp1 KO mice exhibited enamel developmental defects. The enamel volumes of molars and incisors in Wwp1 KO mice [(0.155±0.016), (0.300±0.017) μm³] were reduced by 23.95% (<i>P</i><0.001) and 28.31% (<i>P</i><0.001) compared with the control group [(0.203±0.062), (0.418±0.023) μm³] respectively. Scanning electron microscopy showed disorganized enamel structures in Wwp1 KO incisors and molars. EDS results showed the weight percent of calcium in the enamel rod of incisors decreased in Wwp1 KO mice [(20.74±0.91)%] compared with the control group [(30.30±3.83)%] (<i>P</i><0.001), and the calcium-to-phosphorus ratio decreased in Wwp1 KO mice (1.93±0.01) compared with the control group (2.02±0.01) (<i>P</i><0.001). Immunofluorescence showed weaker AMELX expression in ameloblasts of mandibular first molar tooth germs from P1 and P7 Wwp1 KO mice compared with the control group (<i>P</i><0.001, <i>P</i><0.001). In LS-8 cells, Wwp1 knocked-down led to a decrease of AMELX protein expression, while WWP1 overexpression resulted in an increased AMELX protein level. <b>Conclusions:</b> WWP1 promotes ameloblast differentiation and enamel matrix mineralization, playing a critical role in enamel formation.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"33-42"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Congenital duct orifice ectopia of bilateral submandibular gland with left sublingual gland cyst: a case report].","authors":"J Li, J L Cao, C Y Zhang, J Q Gu","doi":"10.3760/cma.j.cn112144-20240415-00152","DOIUrl":"10.3760/cma.j.cn112144-20240415-00152","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"73-74"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Lacrimal duct obstruction caused by maxillary odontogenic cyst invading lacrimal fossa: a case report].","authors":"H W Xu, P G Du, C Sun, Y H Wang, L Xu, Z H Liu, Q Q Zhang","doi":"10.3760/cma.j.cn112144-20240322-00120","DOIUrl":"10.3760/cma.j.cn112144-20240322-00120","url":null,"abstract":"","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"69-72"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances in tooth development studies based on single-cell RNA sequencing].","authors":"N Jiang, X T Zhu, Y Liu","doi":"10.3760/cma.j.cn112144-20240927-00363","DOIUrl":"10.3760/cma.j.cn112144-20240927-00363","url":null,"abstract":"<p><p>Tooth development is a complex process of the orderly interaction between epithelium originating from the ectoderm and mesenchyme derived from cranial neural crest cells, which not only depends on cell genes regulatory network but also involves crosstalk between cells and their surrounding environment. Even within the same type of cellular populations, obvious heterogeneity may be observed. Single-cell RNA sequencing is a novel technology aimed at sequencing the transcriptome of individual cell. As a critical bridge linking genetic information from the genome to the functional proteome, transcriptome analysis at the single-cell level enables a deeper understanding of cellular heterogeneity and organ developmental trajectories. This approach provides a powerful tool for elucidating complex biological processes and identifying potential therapeutic targets. Here, we summarize the latest advances in employing single-cell RNA sequencing analysis to the process of tooth development, which will help provide insights into a better understanding of prosthodontics and regeneration.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Progress in research on the role of calcium ion transport in dental biomineralization].","authors":"Q Q Liu, D Q Yang","doi":"10.3760/cma.j.cn112144-20241002-00371","DOIUrl":"10.3760/cma.j.cn112144-20241002-00371","url":null,"abstract":"<p><p>Tooth development is a complex biomineralization process formed through the interaction between epithelial tissue and mesenchymal tissue. The Ca²⁺ transport channel is the intrinsic regulatory mechanism of this process, which regulates the concentration of Ca²⁺ inside and outside the cell in time and space, thereby affecting the biological functions of various cells and the growth of extracellular hydroxyapatite. This article reviews the researches on the effects of Ca²⁺ transport in tooth biomineralization and corresponding cell development in recent years, with the aim of providing ideas and basis for the prevention of tooth developmental defects, assisting in early clinical diagnosis, and improving treatment methods.</p>","PeriodicalId":23965,"journal":{"name":"中华口腔医学杂志","volume":"60 1","pages":"81-87"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}