Zeitschrift fur Immunitatsforschung. Immunobiology最新文献_第4页

Human fetal cells. I. Mitogenic responses. 人类胎儿细胞。1 .有丝分裂反应。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

J Faust, F Pranada, H Hapke, H Hörnchen

{"title":"Human fetal cells. I. Mitogenic responses.","authors":"J Faust, F Pranada, H Hapke, H Hörnchen","doi":"","DOIUrl":"","url":null,"abstract":"Human fetal cells from 10 prematures and newborn infants (28--38 weeks of gestational age) and isolated or non-isolated fetal cells circulating in the blood of 9 primigravidae were studied in their ability to respond to phytohemagglutinin, pokeweed, dextran sulfate and lipopolysaccharide. An age-dependent responsiveness of fetal cells obtained from the prematures to all mitogens tested was detected as well as a clear graduation of mitogenic capacity with phytohemagglutinin to produce the highest stimulation. Though a moderate mitogenic response to lipopolysaccharide and dextran sulfate was noted in the blood cultures of the infants, LPS and in part DS transformation of fetal cells obtained from maternal blood appeared to be reduced or absent. A selective stimulation of fetal cells occurring in the circulation of primiparae sufficient for prenatal diagnosis could not be achieved with the mitogens tested. The findings suggest that fetal cells crossing to the mother are different from normal fetal lymphocytes. The present study was performed to elucidate in as quantitative a manner as possible the responses of human fetal cells to different T- and B-cell mitogens. Cells were obtained from various sources for comparing the mitogenic responses of isolated and non-isolated fetal cells. Our results demonstrate that mitogenic responses depend on the gestational age of the fetal cells, the source of the cells and on experimental conditions.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 2","pages":"93-103"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11579741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Determination of the association constant K0 and the number of combining sites n of the mitogenic lectin I and its structural relationship to the nonmitogenic lectin II from the sponge Axinella polypoides (Schmidt) (author's transl)]. [测定海绵Axinella polypoides中有丝分裂凝集素I的结合常数K0和结合位点数目n及其与非有丝分裂凝集素II的结构关系(Schmidt)(作者译)]。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

H Bretting, H Gerstacker

引用次数: 0

Lymphokines. VI. Factors in human and other heterologous sera inhibiting the migration of guinea pig macrophages. 淋巴因子。六、人血清和其他异种血清中抑制豚鼠巨噬细胞迁移的因素。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

R Baer, K Vogt, S Lazary, C Geczy, A L de Weck

{"title":"Lymphokines. VI. Factors in human and other heterologous sera inhibiting the migration of guinea pig macrophages.","authors":"R Baer, K Vogt, S Lazary, C Geczy, A L de Weck","doi":"","DOIUrl":"","url":null,"abstract":"Different heterologous sera including human, rabbit, bovine and fetal calf serum (FCS) showed a strong inhibiting effect on the migration of guinea pig peritoneal macrophages (GPPM), compared to the migration of GPPM in homologous guinea pig serum. The inhibiting effect of these sera on the migration of horse monocytes on the other hand was much less marked. Fractionation of human and rabbit serum showed the 4 S fraction to be most inhibitory on GPPM migration. The migration inhibiting effect of heterologous sera on GPPM was prevented by addition of homologous (GP) serum, by absorption of the sera by various guinea pig cells, by heating at 56 degrees and by addition of alpha-L-fucose. Human sera were found to be strongly cytotoxic for 51Cr-labelled guinea pig erythrocytes and lymphocytes, but not for horse lymphocytes. Upon absorption with guinea pig erythrocytes, lymphocytes and kidney cells, the cytotoxicity of the human sera was strongly reduced. Accordingly, the role of heterophilic antibodies and/or of heat-labile MIF-like factors in heterologous sera has to be considered in al macrophage migration experiments where heterologous sera are being used. These factors may differ from the heat-stable MIF activity generated upon antigen- or mitogen-induced stimulation of cultured lymphocytes.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 2","pages":"130-43"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11946524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The electrophoretic mobility test (EMT) and cancer diagnosis. 电泳迁移率试验(EMT)与癌症诊断。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

F Porzsolt

引用次数: 0

The comparison of different rosette assay systems for the determination of T-lymphocytes in patients with solid malignant tumors. 不同花环试验系统测定实体恶性肿瘤患者t淋巴细胞的比较。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

M Gramatzki, J R Kalden

{"title":"The comparison of different rosette assay systems for the determination of T-lymphocytes in patients with solid malignant tumors.","authors":"M Gramatzki, J R Kalden","doi":"","DOIUrl":"","url":null,"abstract":"The number of T-lymphocytes in the peripheral blood of 51 patients with different type of solid malignancies, 33 normal controls and 10 patients with rheumatoid arthritis was determined using different variations of the sheep red blood cell (SRBC) rosetting-technique (test temperature of 4 or 29 degrees C, medium substituted with or without fetal calf serum [FCS], SRBC treated or untreated with neuraminidase). No significant differences between cancer patients and normal controls were observed in the percentages of T-lymphocytes with the 4 degrees C incubation under any of the conditions tested. In absolute counts, however, a significantly decreased number of T-cells was observed in cancer patients, most likely due to the observed significant lymphopenia in this group. When the test temperature was raised to 29 degrees C, a significantly lower rosette formation was obtained in both percentages and absolute counts of peripheral T-cells in the group of cancer patients as compared to normal individuals only when both neuraminidase treated SRBC and FCS substituted medium were used. The question of whether the observed differences in the percentages and absolute counts of peripheral T-lymphocytes between cancer patients and normal controls using this rosette assay are due to a loss of a T-cell subpopulation or to an alteration in the metabolic state of T-cells in cancer patients remains open.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 2","pages":"104-17"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11524415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of antigens on murine tumor cells reacting with alloantisera against foreign H-2 specificities: analysis by absorption with purified murine leukemia virus and normal lymphoid cells of different H-2 haplotypes. 小鼠肿瘤细胞抗原与异体抗血清对抗外源H-2特异性的特性:用纯化的小鼠白血病病毒和不同H-2单倍型的正常淋巴样细胞吸收分析

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

V Schirrmacher, I Marxen, P Robinson

{"title":"Characterization of antigens on murine tumor cells reacting with alloantisera against foreign H-2 specificities: analysis by absorption with purified murine leukemia virus and normal lymphoid cells of different H-2 haplotypes.","authors":"V Schirrmacher, I Marxen, P Robinson","doi":"","DOIUrl":"","url":null,"abstract":"A spontaneous T cell lymphoma of DBA/2 (H-2-d) mice, SL2, was found to react with anti H-2 typing sera raised against certain foreign haplotypes as well as with anti H-2d sera. The cytotoxic anti-SL2 activity of the anti-foreign H-2 sera was detected in a newly developed microradioassay, not however, in a conventional 51Cr release test. Upon culture in vitro the reactivity of the tumor cells with the anti H-2 sera decreased. The anomalous cytotoxic anti-tumor activity of the anti-foreign H-2 sera appeared to be distinct from anti-murine leukemia virus activity, since it was not removed by absorption with either Friend of AKR leukemia virus. Partial absorption was observed with normal lymphoid cells carrying the respective foreign H-2 antigens, but not with cells of unrelated H-2 haplotypes. In each serum tested, the anti-tumor activity could also be absorbed with syngeneic H-2d lymphoid cells. These results show that the anomalous anti-tumor reactivity of certain anti H-2 typing sera, in particular of sera raised in recipients differing in H-2 from the tumor host strains, is not due to the presence of foreign (derepressed) H-2 molecules on the tumor cells. The differences observed between the tumor cells and normal cells seem to be due to unexpected antibodies in the sera reacting with public H-2 specificities which are better exposed on the tumor cells than on normal cells.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 2","pages":"155-68"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11306110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cooperative pathway induction of T lymphocyte mitogen stimulation. T淋巴细胞有丝分裂原刺激的协同通路诱导。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

J H Peters, L Schimmelpfeng

引用次数: 0

Deviated lysis: lysis of unsensitized cells by complement. V. Generation of the activity of low pH or low ionic strength. 偏离溶解:补体对未致敏细胞的溶解。五、产生低pH或低离子强度的活性。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-12-01

U Rother, G Hänsch, E W Rauterberg, H Jungfer, K Rother

引用次数: 0

Immunoadjuvant effects of the synthetic muramyl-dipeptide (MDP) N-acetylmuramyl-L-alanyl-D-isoglutamine. 合成muramyl-二肽(MDP) n -乙酰muramyl- l- alanyl- d -异谷氨酰胺的免疫佐剂作用。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-10-01

B Heymer, H Finger, C H Wirsing

引用次数: 0

A practical method for quantitation of antibody responses to tetanus and diphtheria toxoids in normal young children. 一种测定正常幼儿破伤风和白喉类毒素抗体反应的实用方法。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1978-10-01

G Virella, H H Fudenberg, K U Kyong, J P Pandey, R M Galbraith

{"title":"A practical method for quantitation of antibody responses to tetanus and diphtheria toxoids in normal young children.","authors":"G Virella, H H Fudenberg, K U Kyong, J P Pandey, R M Galbraith","doi":"","DOIUrl":"","url":null,"abstract":"Antibodies to diphtheria and tetanus toxoids were assayed in 46 children from 16 to 33 months of age following vaccination. A reversed rocket immunoelectrophoretic technique was used for antibody determination. This method is simple and consistent, and although the limit of detection for antibodies of both types (about 1 U/ml) is above the normal range for primary immune responses, antibody levels in immunized children five weeks after a second injection of vaccine were within measureable range in all but two of the children studied. Levels more than one standard deviation above the mean were found in one child for both antitetanus and anti-diphtheria and in four children for anti-tetanus toxoid antibody only. All four of the high responders to tetanus toxoid were Black, carrying both Black and Caucasian Gm haplotypes. The two apparent nonresponders were Caucasian. All children had normal immunoglobulin levels, except for one with low IgA, who showed normal antibody responses to both toxoids. The results suggest that measurements of precipitating antibodies to diphtheria and tetanus toxoids provide a practical index of humoral immune function in small children.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 1","pages":"80-6"},"PeriodicalIF":0.0,"publicationDate":"1978-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11907474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0