{"title":"AET-treated sheep red blood cell and immunobead for combined detection of human peripheral lymphocyte subpopulations.","authors":"Y Lee, Y Yoshizawa, R G Carr, M M Yokoyama","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper describes a method for the simultaneous determination of human thymus-dependent lymphocyte (T cell) and thymus-independent lymphocyte (B cell) populations by mixing lymphocytes, sheep red blood cells, and immunobeads. This method also detects lymphocytes possessing both T and B cell markers. T cells are detected by rosette formation with AET-treated sheep red blood cells and B cells are identified by rosette formation with polyacrylamide gel coated with antibody specific to immunoglobulin classes. The major advantages of this technique is that it is simple, convenient, and reproducible. Three different types of rosettes are distinguishable under light microscopic observation. The detection of double marker lymphocytes, using this combined technique, may have further application in clinical investigation.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"232-9"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11579743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The interaction of opsonins with human polymorphonuclear leucocytes (PMN). I. The influence of human complement (C) and IgG on ingestion and digestion of C-resistant E. coli.","authors":"R Berto, J Menzel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of opsonization on ingestion and digestion of C-resistant E. coli was tested in a phagocytic system consisting of a monolayer of human PMN to which was added E. coli opsonized in different ways. At the end of the phagocytosis, noningested bacteria were separated and the monolayer was removed. The cells were then fractionated. The number of bacteria at different time intervals and the amount and distribution of lysosomal enzymes in the cell fractions were determined. It became apparent that the ingestion of cell-attached bacteria was independent of opsonins. However, the intracellular destruction was enhanced following opsonization with C only, although this was not the result of a greater discharge of lysosomal enzymes into the phagosome. We postulate that sublethal damage of E. coli by C renders the bacteria more sensitive to the bactericidal activity of the phagocytes.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"189-99"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11579742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"C-reactive protein (CRP), 9.5 salpha1-glycoprotein and C1q: serum proteins with lectin properties?","authors":"G Uhlenbruck, D Karduck, H Haupt, H G Schwick","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The human serum proteins C-reactive protein (CRP), the 9.5 Salpha1-glycoprotein and C1q show, when tested in the agar gel diffusion under certain buffer conditions, strong precipitin reactions with polysaccharides of the galactan type, indicating that they may have lectin-like recognition sites.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"262-6"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11630544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J Hamuro, M Röllinghoff, H Wagner, M Seitz, W Grimm, D Gemsa
{"title":"Depressed prostaglandin release from peritoneal cells induced by a T cell adjuvant, lentinan.","authors":"J Hamuro, M Röllinghoff, H Wagner, M Seitz, W Grimm, D Gemsa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>PGE and PGF release from peritoneal exudate cells was studied in mice after injection with two beta (1-3) glucans, the antitumor active lentinan and the inactive pachyman, 4 days after injection of both polysaccharides, the spontaneous and phagocytosis-induced PGE and PGF release was markedly suppressed. However, only the immunopotentiator lentinan induced peritoneal exudate cells which exhibited a longer lasting diminished PG release. The data suggest that the T cell adjuvant lentinan may potentiate cellular immune responses by reducing synthesis of immune suppressive prostaglandins from peritoneal exudate cells.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"248-54"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11433267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Thymic stromal alterations in mice undergoing a chronic graft-versus-host reaction.","authors":"E Potworowski, T Seemayer, R Bolande, W Lapp","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Graft-versus-host induced immunosuppression has previously been shown to be accompanied by severe morphological changes in the thymus; furthermore chronic GvH could become acute by grafting a normal syngeneic thymus, suggesting a functional defect in the autologous thymus. In this work, we monitored the changes occuring in two biologically active thymic stromal fractions during a state of chronic GvH reaction. It was thus observed that a soluble thymic factor (STF), normally found in the reticuloepithelial cells, was lost, and that an insoluble thymic fraction (ITF) found in a double basement membrane surrounding medullary blood vessels, became markedly hypertrophied. These changes are interpreted as being possibly related to the state of immunosuppression by interfering with normal T cell differentiation and traffic through the thymus.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"240-7"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11257053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Alteration of human blood cells and changes in plasma mediators produced by radiographic contrast media.","authors":"J Ring, C M Arroyave","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In vitro incubation of human blood cells with iodinated radiographic contrast media (RCM) produced marked effects which were dose-dependent: erythrocytes showed crenation which was reversible; neutrophil leukocytes released the lysosomal enzyme beta-glucuronidase; basophil leukocytes released histamine; and platelets released serotonin as well as beta-glucuronidase. The release reaction could not be attributed to cell lysis, as demonstrated by the release of the cytoplasmic enzyme lactic dehydrogenase (LDH). In normal human serum, RCM produced activation of the complement system with lysis of cells. This RCM-induced complement activation seemed to occur via the alternate pathway. Stabilizers and cations present in the clinically used RCM solutions did not produce any complement changes.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"200-11"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11306111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AET-treated sheep red blood cell and immunobead for combined detection of human peripheral lymphocyte subpopulations.","authors":"Y. Lee, Y. Yoshizawa, R. Carr, M. Yokoyama","doi":"10.1016/S0340-904X(79)80005-6","DOIUrl":"https://doi.org/10.1016/S0340-904X(79)80005-6","url":null,"abstract":"","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"37 1","pages":"232-9"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78978515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bronchial carcinoma and its regional lymph nodes in relation to immunological functions.","authors":"K. Syrjänen","doi":"10.1016/S0340-904X(79)80003-2","DOIUrl":"https://doi.org/10.1016/S0340-904X(79)80003-2","url":null,"abstract":"","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"119 1","pages":"212-22"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89418671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Alteration of human blood cells and changes in plasma mediators produced by radiographic contrast media.","authors":"J. Ring, C. Arroyave","doi":"10.1016/S0340-904X(79)80002-0","DOIUrl":"https://doi.org/10.1016/S0340-904X(79)80002-0","url":null,"abstract":"","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"30 1","pages":"200-11"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91108353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The interaction of opsonins with human polymorphonuclear leucocytes (PMN). I. The influence of human complement (C) and IgG on ingestion and digestion of C-resistant E. coli.","authors":"R. Berto, J. Menzel","doi":"10.1016/S0340-904X(79)80001-9","DOIUrl":"https://doi.org/10.1016/S0340-904X(79)80001-9","url":null,"abstract":"","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"28 1","pages":"189-99"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80353470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}