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Zeitschrift fur Immunitatsforschung. Immunobiology最新文献

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T and B lymphocytes in the liver and peripheral blood of subjects with acute and chronic hepatitis. 急性和慢性肝炎患者肝脏和外周血中的T淋巴细胞和B淋巴细胞。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
R D'Amelio, M V Ciarla, F Pandolfi, G Panichi, M Sposito, F Aiuti
{"title":"T and B lymphocytes in the liver and peripheral blood of subjects with acute and chronic hepatitis.","authors":"R D'Amelio,&nbsp;M V Ciarla,&nbsp;F Pandolfi,&nbsp;G Panichi,&nbsp;M Sposito,&nbsp;F Aiuti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>E and EAC rosette-forming cells in the peripheral blood and in the liver of subjects with acute and chronic hepatitis were studied. We found a highly significant reduction (P less than 0.01) of E rosette percentage in the lymphocytes isolated from the liver patients with chronic persistent, and chronic active, hepatitis. EAC rosette-forming cells were significantly increased in the liver of patients with chronic active hepatis (P less than 0.01). In this condition lymphocytes with Fc receptor were also found.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"338-45"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11526490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on the relevance of microtubules and of microfilament-dependent processes for triggering lymphocyte activation. 微管和微丝依赖性过程对触发淋巴细胞激活的相关性研究。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
H Lother, E Blitstein-Willinger, T Diamantstein
{"title":"Studies on the relevance of microtubules and of microfilament-dependent processes for triggering lymphocyte activation.","authors":"H Lother,&nbsp;E Blitstein-Willinger,&nbsp;T Diamantstein","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of Isoptin i) on isolated microtubules, ii) on the anti-immunoglobulin and the Concanavalin A induced changes in the plasma membrane topography of murine lymphocytes and iii) on murine lymphocyte activation by lipopolysaccharide and by Concanavalin A was investigated. Isoptin and lidocaine (a local anaestetic) inhibited repolymerisation of tubulin into microtubules and induced depolymerisation of microtubules. Isoptin and lidocaine inhibited competitively binding of colchicine (a classical microtubules disrupting agent) to tubulin. Isoptin induced changes in the plasma membrane topography resembling effects caused by local anaesthetics or by a combination of colchicine and cytochalasin B (an agent affecting microfilament function). Isoptin, lidocaine, colchicine and hydroxyurea when present in the culture medium during the whole incubation period inhibited DNA synthesis induced by lipopolysaccharide or Concanavalin A. RNA synthesis was completely inhibited by lidocaine but not by Isoptin or by colchicine. If Isoptin, colchicine or hydroxyurea were removed from the culture medium at 20 h of culture period, the cells immediately started to incorporate 3H-Thymidine. The inhibitory action of lidocaine was irreversible. These results show that Isoptin, a drug which depolymerizes microtubules in vitro and disturbs the mitogen induced changes in plasma membrane topography of lymphocytes (believed to be controlled by microtubules and microfilaments), does not abolish commitment of the cells for DNA synthesis.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"346-58"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11775592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erythrocyte adherence to the marginal zone of mouse spleen follicle mediated by receptor(s) for neuraminic acid. 神经氨酸受体介导的小鼠脾滤泡边缘区红细胞粘附。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
T Radaszkiewicz, E Weirich, H Denk
{"title":"Erythrocyte adherence to the marginal zone of mouse spleen follicle mediated by receptor(s) for neuraminic acid.","authors":"T Radaszkiewicz,&nbsp;E Weirich,&nbsp;H Denk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Erythrocytes of different species (chicken, sheep, man, mouse, rat, guinea pig) except rabbit erythrocytes strongly adhere to the marginal zone of mouse spleen follicles in frozen sections. This adherence reaction (AR) is not restricted to red blood cells but is also observed with human lymphocytes. Pretreatment of the tissue sections with trypsin, mercaptoethanol, periodate, chloroform/methanol, acetone, and heating the sections abolishes AR whereas neuraminidase (VCN) treatment of the sections has an amplifying effect. AR is inhibited by preincubation of the neuraminidase- or untreated sections with neuraminic acid (NA). Treatment of the erythrocytes with VCN completely abolishes AR whereas treatment with other enzymes (hyaluronidase, collagenase) is ineffective in this respect. Determination of NA in the erythrocyte membrane before and after VCN treatment reveals a positive correlation between the amount of NA and AR. Rabbit red blood cells have the lowest NA content in their membranes and, in addition, there is little effect of VCN treatment in further reducing it. It is possible that a lectin-like substance is responsible for AR. The biologic significance of AR is hypothetical, but since AR occurs in an area of the spleen playing a role in antigen trapping it is conceivable that this trapping may be mediated by interaction of NA and NA receptor(s).</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"319-29"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11668912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Zinc metabolism in normal, lectin-stimulated and leukemic lymphocytes. 锌在正常、凝集素刺激和白血病淋巴细胞中的代谢。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
T Günther, R Averdunk, H Rühl
{"title":"Zinc metabolism in normal, lectin-stimulated and leukemic lymphocytes.","authors":"T Günther,&nbsp;R Averdunk,&nbsp;H Rühl","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gel filtration of the cytoplasmic fraction of homogenate from human peripheral lymphocytes incubated with 65Zn gave five separate peaks with molecular weights of greater than 150,000, 90,000, 65,000, 20,000, and less than 1000 daltons. Peaks I--IV consisted of proteins. The low molecular weight (peak V) may consist of nucleotides, polyamines and amino acids. After gel filtration 75-80% of the 65Zn activity was found in peak V. Lectin-induced stimulation of normal lymphocytes revealed a distribution pattern of 65Zn binding similar to that of unstimulated cells. There was only a slightly enhanced incorporation into the protein peaks I--IV. When peripheral lymphocytes of B lymphocyte origin from patients with chronic lymphocytic leukemia were incubated with 65Zn the same peaks were seen as with supernatants obtained from normal lymphocytes. Lectin-induced stimulation of leukemic lymphocytes had no significant effect on the 65Zn distribution pattern.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"269-78"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11668910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stimulation of humoral immunity by peptidoglycan monomer from Brevibacterium divaricatum. 短杆菌肽聚糖单体对体液免疫的刺激作用。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
I Hrsak, J Tomasić, K Pavelić, Z Valinger
{"title":"Stimulation of humoral immunity by peptidoglycan monomer from Brevibacterium divaricatum.","authors":"I Hrsak,&nbsp;J Tomasić,&nbsp;K Pavelić,&nbsp;Z Valinger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peptidoglycan monomer (PGM), a water soluble and nontoxic disaccharide pentapeptide unit obtained from Brevibacterium divaricatum, was administered intravenously into mice, and the humoral immune response to sheep erythrocytes was assayed by means of Jerne's technique for plaque-forming cells (PFC) in the spleen. The PFC response was evidently stimulated. The counts were increased to practically the same extent over a great range of doses of PGM (from 25 to 1600 microgram per animal), and the effect was present in the mice immunised with optimal, as well as in those immunised with suboptimal, doses of antigen. The magnitude of the immunostimulation depend only on the timing of PGM administration: it was maximal if PGM was injected 1 or 2 days after the antigen. In vitro, in a 4-day culture of spleen cells, PGM did not stimulate PFC formation. We conclude that stimulation of the humoral immune response to sheep red blood cell antigens by PGM probably occurs without cell multiplication and probably involves more than simply a contact of immunocompetent cells with PGM.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"312-8"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11588188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spontaneous cell-mediated cytotoxicity (SCMC) associated with lymphocytes negative for acid alpha-naphthyl acetate esterase (ANAE) activity. 自发性细胞介导的细胞毒性(SCMC)与酸性-乙酸萘酯酶(ANAE)活性阴性的淋巴细胞相关。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
V Rajvanshi, H H Peter, H J Avenarius
{"title":"Spontaneous cell-mediated cytotoxicity (SCMC) associated with lymphocytes negative for acid alpha-naphthyl acetate esterase (ANAE) activity.","authors":"V Rajvanshi,&nbsp;H H Peter,&nbsp;H J Avenarius","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A modified histochemical procedure for nonspecific acid alpha-naphthyl acetate esterase (ANAE) activity in human lymphocytes was used to identify a subpopulation of E-rosette forming cells. Performing a one hour reaction at pH 6.5 the distinct dot-like staining pattern was almost exclusively observed on high affinity E-rosettes which sedimented readily in a regular Ficoll-Metrizoate gradient. By combining latex phagocytosis with staining for ANAE activity, a clear-cut distinction between mononuclear phagocytes and lymphocytes could be made. An attempt was undertaken to relate the ANAE marker on human lymphocytes to their functional capacity in spontaneous cell-mediated cytotoxicity (SCMC) reactions. Using as targets two allogeneic (K562,IGR3) and a xenogeneic cell line (L1210) it could be clearly demonstrated that high SCMC activity is mediated by ANAE negative mononuclear cells, whereas enrichment for ANAE positive lymphocytes resulted in a loss of SCMC.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"330-7"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11309113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
75Se-release: a short and long term assay system for cellular cytoxicity. 硒释放:一种细胞毒性的短期和长期测定系统。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-03-01
W Leibold, S Bridge
{"title":"75Se-release: a short and long term assay system for cellular cytoxicity.","authors":"W Leibold,&nbsp;S Bridge","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The gamma-emitting aminoacid 75Se-selenomethionine (75SeM) was examined as a target cell label in cytotoxic assays. It was efficiently taken up by activated, intensively metabolizing cells of various types but hardly at all by resting or low-metabolizing cells. Culturing activated cells in methionine-deficient medium with 3--5 mu Ci75SeM/ml for 18--22 h usually resulted in an uptake of 3--20 cpm/cell which was 3--200 times that of 51Cr marked cells. 75SeM-labelled cells kept in medium at ambient temperature or at 37 degrees C, maintained a high radioactivity per cell and a viability above 85% for at least 72 h without significant increase in spontaneous isotope release or loss in sensitivity in subsequent cytotoxic tests. 75Se-labelled material released from target cells was not reutilized by unlabelled lymphoid cells. Provided the cells were carefully washed after labelling and kept in optimal culture conditions, the reasonably low baseline release (usually 0.6--1.8% of input/h) in the medium control allowed performance of long-term assays of up to 54 h. However, strong cytotoxic reactions (e.g. ADCC) could cause over 50% specific 75Se-release within 5 h. With constant amounts of effector cells (3.6 x 10(3) up to 3 x 10(5)/well) identical or even higher, specific releases were obtained on 6 x 10(2) targets as compared to 1 x 10(4) targets/well. Thus, the 75Se-release assay offers a single monitoring system suitable for short (3--6 h) and long term (usually up to 44 h) cytotoxic reactions on a microscale, using 1 x 10(3) or less targets/well. Its sensitivity permits evaluation of strong and weak reactions as well as early and delayed onset cytotoxicity. In addition, with a gamma-spectrometer the radioactivity of 75Se can easily be distinguished from that of 51Cr. Due to this, and an improved method for 51Cr labelling of cells (10 mu Ci 51Cr/ml medium for 18--22 h), a double gamma-labelling of cellular proteins is available which provides new possibilities for monitoring cellular interactions in short and long term tests.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 4","pages":"287-311"},"PeriodicalIF":0.0,"publicationDate":"1979-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11668911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibodies against purified pigeon IgA in pigeon breeders' disease. 纯化鸽IgA抗体在鸽饲养者病中的应用。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-02-01
G de Ridder, J Goudswaard, L Berrens
{"title":"Antibodies against purified pigeon IgA in pigeon breeders' disease.","authors":"G de Ridder,&nbsp;J Goudswaard,&nbsp;L Berrens","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is demonstrated that previously described PDF1-A antigens from pigeon dropping extract contain pigeon IgA. The sera of patients with pigeon breeders' disease contain precipitating antibodies against pigeon IgA, in contrast to the sera of pigeon breeders suffering from unrelated forms of pulmonary dysfunction. The degree of complement consumption by PDF1A antigens, pigeon serum, and pigeon IgA turned out to be correlated. No correlation was found between precipitating anti-pigeon IgA antibodies and complement consumption by pigeon IgA in patients' sera.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"223-31"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11525248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bronchial carcinoma and its regional lymph nodes in relation to immunological functions. 支气管癌及其区域淋巴结与免疫功能的关系。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-02-01
K J Syrjänen
{"title":"Bronchial carcinoma and its regional lymph nodes in relation to immunological functions.","authors":"K J Syrjänen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two hundred and fifty lymph nodes from 100 patients with lung carcinoma and the same number of regional nodes from 119 patients with gastric ulcer were histologically evaluated by the standardized reporting system of COTTIER et al. Special attention was focused on the immunological reactions in these lymph nodes. Histologic features suggesting actively functioning humoral and cell-mediated immune reactions were encountered more frequently in the nodes of the control series. The significance of the histologic findings is discussed and it is concluded that there is some degree of derangement of both humoral and cell-mediated immune responses in the lymph nodes draining lung carcinoma. The applicability of the reporting system used is emphasized.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"212-22"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11524416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temperature-induced dissociation of serum amyloid protein SAA. 温度诱导的血清淀粉样蛋白SAA解离。
Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1979-02-01
R P Linke
{"title":"Temperature-induced dissociation of serum amyloid protein SAA.","authors":"R P Linke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serum amyloid A protein (SAA) has been shown to be unstable at elevated temperatures. While in agreement with earlier reports, molecular weights of approximately 180,000 daltons were determined by gel filtration at 37 degrees C and below, above 58 degrees C additional AA-antigenic activity was found in the void volume and at the position of approximately 12,000 daltons. The latter polypeptide resembled in size and immunoreactivity SAAL in that it had AA-antigenic determinants exposed at 4 degrees C (unlike SAA). Because the release of SAAL or a similar molecule could not be prevented by the enzyme inhibitor PMSF, a temperature-dependent dissociation of SAA is proposed. In view of the known occurrence of amyloid following febrile conditions, the change in size and immunoreactivity of AA-antigenic proteins in vitro may indicate that a similar mechanism in vivo is important in the genesis of AA-type amyloidosis.</p>","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"155 3","pages":"255-61"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11306112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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