World Mycotoxin Journal最新文献

{"title":"Aflatoxin contamination in maize: occurrence and health implications in Latin America","authors":"N. Ponce‐García, N. Palacios-Rojas, S. Serna-Saldívar, S. García‐Lara","doi":"10.3920/wmj2020.2666","DOIUrl":"https://doi.org/10.3920/wmj2020.2666","url":null,"abstract":"According to the United Nations Food and Agriculture Organisation, mycotoxicoses constitute the second most pressing food safety problem worldwide, with most cases occurring in developing countries. Maize (Zea mays L.), the main staple for many Latin Americans, is one of the best suitable substrates for mycotoxigenic Aspergillus fungi. Aflatoxins (AFs) produced primarily by Aspergillus flavus, are of significant concern, especially in developing countries. While AFs production occurs mainly in warmer, tropical, and subtropical environments, recent evidence suggests that global climate change favours their presence in regions with little or no awareness of this issue. AFs interfere with metabolic processes, causing cancer and other health disorders resulting in health hazards and even death. The setting of national acceptable regulatory levels of AFs is necessary for Latin American countries. Unfortunately, no estimates of the economic impact of AFs in this region are currently available nor the cost of regulatory programs designed to reduce health risks to animals and humans. This review explores relevant data about incidence of AFs in maize produced in the region and the adverse effects of the consumption of contaminated foods and the associated health consequences for Latin American consumers. Regulations aimed to mitigate AFs exposure to consumers are also reviewed and identified gaps for researchers and actors of the maize value chain are also proposed.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43062337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary aflatoxin on biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish","authors":"E. C. Michelin, C. M. Bedoya-Serna, L. C. S. Carrion, N. Levy-Pereira, F. S. Cury, D. Passarelli, C. G. Lima, G. Yasui, R. Sousa, A. M. Fernandes","doi":"10.3920/wmj2020.2662","DOIUrl":"https://doi.org/10.3920/wmj2020.2662","url":null,"abstract":"The objective of this study was to evaluate biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins. Fish feed was artificially contaminated with aflatoxins and the treatments were: Control – feed without toxin; Treatment A – feed + 10 μg aflatoxin B1 (AFB1)/kg; Treatment B – feed + 20 μg AFB1/kg; and Treatment C – feed + 50 μg AFB1/kg. Matrinxã and Pacu juvenile fish were placed in tanks for 180 days. Five experimental units per treatment were monthly sampled and submitted to blood collection and removal of hepatic tissue. Thus, twenty blood and liver samples for each species were collected monthly, adding up to 240 samples analysed. To verify biochemical changes, analyses included total proteins, albumin, globulins, aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The hepatic tissue was examined microscopically and the slides presenting histopathological changes were photo-documented. There was effect of treatment (P<0.05) for AST and ALP in Matrinxã, while no effect (P>0.05) was observed in Pacu. A reduction (P<0.05) in AST and ALP values during the time of exposure was observed in all treatments for both species. Fatty degeneration and liver damage were observed for both species in treatments exposed to aflatoxins. Fatty degeneration in Pacu was noticed after 30 days of exposure, while in Matrinxã it was observed after 60 days. Disorganisation of the hepatocyte cord arrangement was also observed in those treatments exposed to aflatoxin, following 90 days of exposure in Matrinxã, and after 60 days in Pacu. Therefore, aflatoxins have little influence on biochemical parameters in the species evaluated. However, exposure to aflatoxins caused liver changes, such as cell death, fatty and hydropic degeneration, thus it could be concluded that both species are susceptible to the toxic effects of long-term exposure to dietary AFB1.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41774337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxin B1 contamination of feedstuff on a dairy farm in Northern Peru and aflatoxin M1 concentrations in raw milk","authors":"I. Salazar, I. López, P. Glorio‐Paulet, C. Gómez","doi":"10.3920/wmj2020.2672","DOIUrl":"https://doi.org/10.3920/wmj2020.2672","url":null,"abstract":"Research regarding aflatoxin contamination levels in Peru is limited, although aflatoxin M1 (AFM1) and aflatoxin B1 (AFB1) require surveillance because of their toxicity. European regulations state that the harmonised maximum level (ML) is 5 μg/kg for AFB1 in feedstuffs and 0.05 μg/kg for AFM1 in milk. Our study aimed to determine the annual variation levels of AFB1 in ingredients used in feedstuffs for dairy cows and those of AFM1 in milk at a typical intensive dairy farm in Northern Peru. For 1 year, milk (n=529) and feedstuff samples (n=235) were collected and aflatoxin levels were determined using a lateral flow immunoassay. We found that 16% of milk samples had AFM1 contamination above the ML. AFM1 level was significantly higher (P<0.05) in December (end of spring) than that in all other months. Throughout the year, the most used feedstuffs were maize, soybean meal and whole soybean. Among the maize samples (n=77), 2.59% had an AFB1 level above the ML, whereas 45% had an AFB1 level below the ML. On the other hand, neither the soybean meal (n=69) nor whole soybean samples (n=64) had an AFB1 level above the ML, 46.4 and 20%, respectively. In 50% (n=10) of cottonseed meal samples, AFB1 level was above the ML; in 20% of wheat middling samples, it was above the ML. Cottonseed and wheat middling samples were used for 2 and 5 months, respectively. AFB1 level in feedstuff showed a significant difference in December (P<0.05) compared with other months, specifically for maize and soybean meal. As the AFM1 level in milk results from AFB1 contaminated feedstuff, our results emphasise the need to implement specific quality measures to reduce contamination.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48506110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of aflatoxin M1 contamination in milk and cheese produced in Mexico: a review","authors":"C. Jiménez-Pérez, S. Alatorre-Santamaría, S. Tello-Solís, L. Gómez‐Ruiz, G. Rodríguez-Serrano, M. García‐Garibay, A. Cruz-Guerrero","doi":"10.3920/wmj2020.2668","DOIUrl":"https://doi.org/10.3920/wmj2020.2668","url":null,"abstract":"Due to the carcinogenic character of aflatoxins when present in foods, these compounds are considered a risk to human health. This systematic review aimed at compiling the available research data on detection and quantification of aflatoxin M1 (AFM1) in milk and common types of cheese produced in Mexico in the past two decades. A limited number of studies were found that matched the purpose of our review. Only ten research works focused on the evaluation of AFM1 content in milk while three studies analysed the occurrence of this mycotoxin in oaxaca and panela cheeses. HPLC-FD and ELISA were the methods of choice utilised to detect AFM1. Concentrations higher than 0.5 μg AFM1/kg, a maximum limit set in current food regulation in Mexico, were found in major dairy brands consumed in Mexico. Analysis of raw milk produced during the rainy season in the states of Jalisco (2007) and Chiapas (2013) showed mycotoxin levels within the regulation limits while milk samples obtained during the dry season in the Mexico City and the State of Mexico (2008) exceeded that threshold. For cheeses, 33% of the artisanal produced oaxaca type samples from Veracruz (2016) and 55% of those acquired in Mexico City (2019) were found above the limit set for milk. In contrast, the panela cheese samples obtained in Baja California and Guanajuato (2009) complied with the AFM1 regulation. Additionally, the presence of AFB1 and its hydroxylated metabolites other than AFM1 were determined in the major milk brands at concentrations that could be of high risk for human health. Similar results were reported for both artisan and industrially produced oaxaca cheese. Finally, mycotoxins enter human food chain through animals fed with contaminated fodder. Our systematic review demonstrated the urgent need to amend the existing food regulation in Mexico to include mycotoxins as potent contaminants in cheese.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":"1 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41730241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomonitoring aflatoxin B1 exposure of residents from the Amazon region: a pilot study","authors":"K. Higashioka, A. Kluczkovski, E. S. Lima, A. Lucas","doi":"10.3920/WMJ2020.2627","DOIUrl":"https://doi.org/10.3920/WMJ2020.2627","url":null,"abstract":"The present study was a pilot study that aimed to evaluate the occurrence of aflatoxins (AF) in the human diets and its presence in human urine as a metabolite (aflatoxin M1; AFM1). Volunteers from...","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46272101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-species variability in Fusarium langsethiae strains in growth and T-2/HT-2 mycotoxin production in response to climate change abiotic factors","authors":"C. Verheecke-vaessen, A. Lopez-Pietro, E. García-Cela, Á. Medina, N. Magan","doi":"10.3920/wmj2020.2584","DOIUrl":"https://doi.org/10.3920/wmj2020.2584","url":null,"abstract":"The objective of this study was to evaluate the potential intra-species variability of 3 Fusarium langsethiae strains in response to extreme climate change (CC) conditions on an oat-based matrix. The impact of elevated temperature (25 vs 30-34 °C) coupled with increasing drought stress (0.98 vs 0.95 aw) and elevated CO2 (400 vs 1000 ppm) were examined on lag phases prior to growth, growth rate, and production of the mycotoxins T-2 and HT-2 and their ratio. In comparison to the control conditions (25 °C; 0.98; 400 ppm), exposure to increased temperature (30-34 °C), showed similar reductions in the lag phase and fungal growth rates of all 3 strains. However, with elevated CO2 a reduction in both lag phases prior to growth and growth rate occurred regardless of the aw examined. For T-2 and HT-2 mycotoxin production, T-2 showed the most intra-species variability in response to the interacting abiotic stress factors, with the 3 strains having different environmental conditions for triggering increases in T-2 production: Strain 1 produced higher T-2 toxin at 25 °C, while Strain 2 and the type strain (Fl201059) produced most at 0.98 aw/30 °C. Only Strain 2 showed a reduction in toxin production when exposed to elevated CO2. HT-2 production was higher at 25 °C for the type strain and higher at 30-34 °C for the other two strains, regardless of the aw or CO2 level examined. The HT-2/T-2 ratio showed no significant differences due to the imposed interacting CC abiotic conditions.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44555745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of patulin certified reference material using mass balance and quantitative NMR","authors":"X. Li, H. Li, Wen Zhang, Qi Zhang, Z. Guo, S. Song, G. Zhao","doi":"10.3920/wmj2021.2691","DOIUrl":"https://doi.org/10.3920/wmj2021.2691","url":null,"abstract":"The certified reference materials (CRMs) are necessary for accurate quantification and insurance of comparability and traceability of results. Patulin is a typical mycotoxin in a variety of food commodities. Here, patulin CRM GBW(E)100673 was characterised and its purity was assessed by two independent orthogonal approaches including mass balance (MB) and quantitative nuclear magnetic resonance spectroscopy (qNMR) methods. From MB equation, the calculated purity was 996.9 mg/g with subtraction of water, volatile solvent, inorganic and structurally related impurities. In the other qNMR method, the calculated purity was 996.7 mg/g. This CRM was homogeneous and stable for at least 9 months under -20 °C in dark. Finally, a purity of 997 mg/g with an expanded uncertainty of 3 mg/g (k=2) was finally assigned to patulin CRM in this study. High-purity patulin CRM was fully characterised and assessed for the first time. The new CRM can be applicable to routine monitoring and risk assessment for assurance of accuracy results in food safety.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46213380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary exposure to mycotoxins of 1- and 2-year-old children from a Dutch Total Diet Study","authors":"A. Pustjens, J. Castenmiller, J. D. Biesebeek, T. C. Rijk, Ruud C. J. Dam, P. Boon","doi":"10.3920/wmj2020.2676","DOIUrl":"https://doi.org/10.3920/wmj2020.2676","url":null,"abstract":"In 2017, a Total Diet Study was conducted in the Netherlands in which mycotoxins were analysed in foods and beverages consumed by 1- and 2-year-old children. These mycotoxins were aflatoxins, Alternaria toxins, citrinin, ergot alkaloids, fumonisins, ochratoxin A, patulin, sterigmatocystin, trichothecenes, and zearalenone. Long-term exposure was calculated by combining concentrations in foods and beverages with consumed amounts of these products. Analysed foods and beverages with a concentration below the detection limit that could contain the mycotoxin, were assigned a concentration equal to half this limit value. To assess if the exposure could result in a possible health risk, the high long-term exposure (95th percentile) was compared with a health-based guidance value (HBGV) or a margin of exposure (MOE) was calculated. Exposure to aflatoxins, Alternaria toxins, ochratoxin A and T-2/HT-2 sum may pose a health concern. Foods that contributed most to the exposure of these mycotoxins were bread, biscuits, breakfast cereals, chocolates, dried fruit, follow-on formula and fruit juices.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45154099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of aflatoxin B1 adducts in Mexican women with cervical lesions","authors":"L. León-Martínez, C. M. López-Mendoza, Y. Terán-Figueroa, R. Flores-Ramírez, F. Díaz-Barriga, L. E. Alcántara-Quintana","doi":"10.3920/WMJ2020.2602","DOIUrl":"https://doi.org/10.3920/WMJ2020.2602","url":null,"abstract":"Cervical cancer (CC) is one of the most serious threats to the lives of women; co-factors in addition to oncogenic human papillomavirus (HPV) infection may be important in causing CC. Women in Mexi...","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46212481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of an analytical method based on QuEChERS and LC-MS/MS to quantify nine mycotoxins in plant-based milk","authors":"L. Pinto, A. Santos, E. Vargas, F. Madureira, A. Faria, R. Augusti","doi":"10.3920/WMJ2020.2656","DOIUrl":"https://doi.org/10.3920/WMJ2020.2656","url":null,"abstract":"Plant-based beverages (popularly known as vegetable milk) have become increasingly important in recent years. However, the nonexistence of information on mycotoxin contamination is noticeable. We h...","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":"1 1","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47208500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}