World Mycotoxin Journal最新文献

{"title":"Antibody conjugated magnetic nanoparticle based colorimetric assay for the detection and quantification of aflatoxin B1 in wheat grains","authors":"R. Kumari, H. Jaiswal., Trinath Chowdhury, A. Ghosh","doi":"10.3920/wmj2021.2687","DOIUrl":"https://doi.org/10.3920/wmj2021.2687","url":null,"abstract":"Aflatoxin B1 (AFB1) is a most potent carcinogenic secondary metabolite produced by Aspergillus flavus. As a food safety concern, development of a rapid, cost effective, sensitive and easy to use method for the detection of aflatoxin is of prime requirement. In this study, AFB1 was conjugated with bovine serum albumin (BSA), and AFB1-BSA conjugate was purified by HPLC. Purification was confirmed by UV-Vis spectroscopy, FTIR and MALDI-TOF mass spectrometry. The polyclonal antibody was raised against AFB1-BSA conjugate in rabbit and purified by protein A sepharose and BSA sepharose affinity columns. Iron oxide nanoparticles (MNPs) were synthesised by co-precipitation method and their surface was functionalised with (3-aminopropyl) triethoxysilane (APTES). Size of APTES conjugated MNPs was determined by electron microscopy, and characterised by several biophysical techniques. The purified anti-AFB1 antibody was conjugated with surface functionalised MNPs and the conjugation was confirmed by determining the sizes of free and antibody conjugated MNPs by field emission scanning electron microscope where increase of particle sizes from 10-20 to 40-50 nm was observed due to antibody conjugation. Anti-AFB1 antibody conjugated MNPs were used for capturing AFB1 from the aflatoxin spiked wheat grains with a recovery percentage of more than 80% and used effectively five times. The captured AFB1 was then quantified by a sensitive colorimetric assay where colourless AFB1 was first converted into coumaric acid by NaOH. Subsequently, coumaric acid reacted with 2,6-dibromoquinone-4-chloroimide (DBQC) to a green-coloured indophenol product which was quantified spectrophotometrically. AFB1 contamination as low as 2 μg/kg in wheat grains was detected by the developed technique suggesting its potential application for both qualitative and quantitative analysis of aflatoxins present in feed and food materials.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48855675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decision support system for integrated management of mycotoxins in feed and food supply chains","authors":"H. J. van der Fels-Klerx, C. Liu, M. Focker, I. Montero-Castro, V. Rossi, V. Manstretta, N. Magan, R. Krska","doi":"10.3920/wmj2020.2603","DOIUrl":"https://doi.org/10.3920/wmj2020.2603","url":null,"abstract":"Mycotoxins present a global food safety threat of our feed and food. Mycotoxins are toxic metabolites of certain fungi in agricultural products that are harmful to animal and human health. The presence of mycotoxins in these products depends on a variety of management and environmental factors in the field, during storage and/or processing of feed and food commodities. To date, information on mycotoxin management is available, but is not easy to access by supply chain actors. This study aimed to design, build and test a Decision Support System (DSS) that can help decision making on mycotoxin management by various actors along the feed and food supply chains. As part of this, available knowledge and data on mycotoxin prevention and control were collected and synthesised into easy to understand guidelines and tools for various groups of end-users. The DSS consists of four different modules: (a) static information module and (b) scenario analysis module, (c) dynamic module for forecasting mycotoxins, and (d) dynamic module for real-time monitoring of moulds/mycotoxins in grain silos. Intended end-users are all end-user groups for modules (a) and (b); growers and collectors for module (c) and; post-harvest storage managers for module (d). The DSS is user-friendly and accessible through PCs, tablets and smartphones (see https://mytoolbox-platform.com/ ). In various phases of the DSS development, the tool has been demonstrated to groups of end-users, and their suggestions have been taken into account, whenever possible. Also, a near final version has been tested with individual farmers on the easiness to use the system. In this way we aimed to maximise the DSS uptake by actors along the chain. Ultimately, this DSS will improve decision making on mycotoxin management; it will assist in reducing mycotoxin contamination in the key crops of Europe, thereby reducing economic losses and improving animal and human health.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44732444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat-induced reduction of deoxynivalenol and its modified forms during flaking and cooking of oat","authors":"Anastasia S. Hole, I. Rud, S. Sahlstrøm, Lada Ivanova, G. Eriksen, H. Divon","doi":"10.3920/wmj2020.2661","DOIUrl":"https://doi.org/10.3920/wmj2020.2661","url":null,"abstract":"Deoxynivalenol (DON) and its modified forms deoxynivalenol-3-glucoside (DON-3G) and 3-acetyl-deoxynivalenol (3-ADON) are common contaminants in Norwegian oats. In order to provide more information about the fate of these mycotoxins during oat processing, the levels of DON, DON-3G, 3-ADON and the sum of them (total DON) were determined using LC-HRMS/MS at different processing steps. Oat groat was softened by either steaming or conditioning, rolled into flakes of two thicknesses, and subsequently cooked to produce flake porridges. Flour of oat groat (untreated or kilned) was cooked to flour porridges. The flaking process had major effect on the mycotoxin levels in resulting flakes, with significant impact for type of softening regime, but not for flake size. Steam-softening caused the largest reduction of DON, DON-3G and total DON in flakes, retaining 41, 60 and 46%, respectively, compared to oat groat. In contrast, 3-ADON in flakes was most reduced by conditioning, to 29% of the levels in oat groat. Cooking to porridge from flakes did not result in any additional mycotoxin reduction, though significant impact of flake size was shown in the final porridges, with highest reduction of total DON in the porridges originating from steamed thick flakes. Cooking porridge from untreated oat flour gave significant reduction in mycotoxin levels, however not for kilned oat flour which had already undergone reduction during kilning. In conclusion, the study shows that processes involving heat-treatment, i.e. kilning, steaming or cooking, efficiently reduced total DON in oats during flaking and porridge cooking, and reduction is dependent on previous processing steps.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47547783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India","authors":"H. Thukral, Pankaj Dhaka, J. Bedi, R. Aulakh","doi":"10.3920/wmj2021.2697","DOIUrl":"https://doi.org/10.3920/wmj2021.2697","url":null,"abstract":"Aflatoxin M1 (AFM1) contamination in milk and milk products may pose a major public health concern. The present cross-sectional study was aimed to estimate the prevalence of AFM1 in bovine milk across all districts of Punjab, India and to identify the associated animal and farm level risk factors. A total of 402 milk samples (266 cow milk and 136 buffalo milk) were analysed using commercial ELISA and representative samples were confirmed using HPLC-FLD. The results revealed that 56.2 and 13.4% of the milk samples exceeded the maximum levels of the European Union, i.e. 0.05 μg/l and Food Safety and Standards Authority of India (FSSAI), i.e. 0.5 μg/l for AFM1 in milk, respectively. On analysis of species variation, buffalo milk (prevalence: 56.6%; mean concentration: 0.42±0.9 μg/l) was found to have higher AFM1 levels than cow milk (prevalence: 56.0%; mean concentration: 0.19±0.3 μg/l), with statistically significant difference between mean concentrations (P<0.01) and non-significant difference between AFM1 prevalence (P=0.91). Furthermore, milk from commercial dairy farms (prevalence: 64.7%; mean concentration: 0.34±0.65 μg/l) was found to be more contaminated than from household dairy establishments (prevalence: 47.8%; mean concentration: 0.19±0.65 μg/l). The risk factors ‘above average milk yield/day’ (odds ratio (OR): 2.4) and ‘poor animal hygiene’ (OR: 1.9) were identified at animal level, and ‘intensive dairy farming’ (OR: 3.1) and ‘animal feed without aflatoxin binder’ (OR: 4.7) as farm level risk factors for AFM1 excretion above maximum levels of European Union in milk. Among cow breeds, the milk from ‘non-descript’ breed (OR: 11.5) was found to be most contaminated with AFM1 and the least from Jersey breed (OR: 1.0). The present study highlighted the presence of AFM1 in milk samples; therefore, regular monitoring of AFM1 in milk is required so that high risk regions and associated risk factors can be addressed appropriately.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46237151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential for aflatoxin predictive risk modelling in sub-Saharan Africa: a review","authors":"B. Keller, T. Russo, F. Rembold, Y. Chauhan, P. Battilani, A. Wenndt, M. Connett","doi":"10.3920/wmj2021.2683","DOIUrl":"https://doi.org/10.3920/wmj2021.2683","url":null,"abstract":"This review presents the current state of aflatoxin risk prediction models and their potential for value actors throughout the food chain in sub-Saharan Africa, with a specific focus on improving smallholder farmer management practices. Several empirical and mechanistic models have been developed either in academic research or by private sector aggregators and processors in high-income countries including Australia, the USA, and Southern Europe, but these models have been only minimally applied in sub-Saharan Africa, where there is significant potential and increasing need due to climate variability. Predictions can be made based on historic occurrence data using either a mechanistic microbiological framework for aflatoxin accumulation or an empirical model based on statistical correlations with climate conditions and local agronomic factors. Model results can then be distributed to smallholders through private, public, or mobile extension services, used by policymakers for strategy or policy, or utilised by private sector institutions for management decisions. Specific agricultural advice can be given during the three most critical points in the phenological cycle: preseason insight including sowing timing and crop varieties, preharvest advice about management and harvest timing, and postharvest optimal practices including storage, drying, and market information. Model development for sub-Saharan Africa is limited by a dearth of georeferenced aflatoxin occurrence data and real-time high resolution climate data; the wide diversity of farm typologies each with significant information and technology gaps; a prevalence of informal market structures and lack of economic incentives systems; and general lack of awareness around aflatoxins and best management practices to mitigate risk. Given advancements towards solving these challenges, predictive aflatoxin models can be integrated into decision support platforms to focus on optimisation of value for smallholders by minimising yield and nutritional losses, which can propagate value throughout the production and postharvest phases.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41823211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of deoxynivalenol exposure at peripuberty over testicles of rats: structural and functional alterations","authors":"J. Gerez, A. Gomes, R. Erthal, G. S. Fernandes, Ricardo de Matos, W. Verri, E. M. Gloria, A. Bracarense","doi":"10.3920/WMJ2020.2667","DOIUrl":"https://doi.org/10.3920/WMJ2020.2667","url":null,"abstract":"Deoxynivalenol (DON) is related to reduced reproductive performance in males and females in several species. Children and adolescents showed a high risk of exposure to DON, however, no study has ev...","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45059082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and concentration of mycotoxins in maize dried distillers’ grains produced in Brazil","authors":"C. Mallmann, C. Simões, J. Vidal, C. Silva, L. M. D. L. Schlösser, C. A. Almeida","doi":"10.3920/WMJ2020.2669","DOIUrl":"https://doi.org/10.3920/WMJ2020.2669","url":null,"abstract":"The presence of mycotoxins in dried distillers’ grains with solubles (DDGS), a by-product of bioethanol production from maize, has been a matter of concern due to the increasing global utilisation ...","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43394645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxin contamination in maize: occurrence and health implications in Latin America","authors":"N. Ponce‐García, N. Palacios-Rojas, S. Serna-Saldívar, S. García‐Lara","doi":"10.3920/wmj2020.2666","DOIUrl":"https://doi.org/10.3920/wmj2020.2666","url":null,"abstract":"According to the United Nations Food and Agriculture Organisation, mycotoxicoses constitute the second most pressing food safety problem worldwide, with most cases occurring in developing countries. Maize (Zea mays L.), the main staple for many Latin Americans, is one of the best suitable substrates for mycotoxigenic Aspergillus fungi. Aflatoxins (AFs) produced primarily by Aspergillus flavus, are of significant concern, especially in developing countries. While AFs production occurs mainly in warmer, tropical, and subtropical environments, recent evidence suggests that global climate change favours their presence in regions with little or no awareness of this issue. AFs interfere with metabolic processes, causing cancer and other health disorders resulting in health hazards and even death. The setting of national acceptable regulatory levels of AFs is necessary for Latin American countries. Unfortunately, no estimates of the economic impact of AFs in this region are currently available nor the cost of regulatory programs designed to reduce health risks to animals and humans. This review explores relevant data about incidence of AFs in maize produced in the region and the adverse effects of the consumption of contaminated foods and the associated health consequences for Latin American consumers. Regulations aimed to mitigate AFs exposure to consumers are also reviewed and identified gaps for researchers and actors of the maize value chain are also proposed.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43062337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary aflatoxin on biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish","authors":"E. C. Michelin, C. M. Bedoya-Serna, L. C. S. Carrion, N. Levy-Pereira, F. S. Cury, D. Passarelli, C. G. Lima, G. Yasui, R. Sousa, A. M. Fernandes","doi":"10.3920/wmj2020.2662","DOIUrl":"https://doi.org/10.3920/wmj2020.2662","url":null,"abstract":"The objective of this study was to evaluate biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins. Fish feed was artificially contaminated with aflatoxins and the treatments were: Control – feed without toxin; Treatment A – feed + 10 μg aflatoxin B1 (AFB1)/kg; Treatment B – feed + 20 μg AFB1/kg; and Treatment C – feed + 50 μg AFB1/kg. Matrinxã and Pacu juvenile fish were placed in tanks for 180 days. Five experimental units per treatment were monthly sampled and submitted to blood collection and removal of hepatic tissue. Thus, twenty blood and liver samples for each species were collected monthly, adding up to 240 samples analysed. To verify biochemical changes, analyses included total proteins, albumin, globulins, aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The hepatic tissue was examined microscopically and the slides presenting histopathological changes were photo-documented. There was effect of treatment (P<0.05) for AST and ALP in Matrinxã, while no effect (P>0.05) was observed in Pacu. A reduction (P<0.05) in AST and ALP values during the time of exposure was observed in all treatments for both species. Fatty degeneration and liver damage were observed for both species in treatments exposed to aflatoxins. Fatty degeneration in Pacu was noticed after 30 days of exposure, while in Matrinxã it was observed after 60 days. Disorganisation of the hepatocyte cord arrangement was also observed in those treatments exposed to aflatoxin, following 90 days of exposure in Matrinxã, and after 60 days in Pacu. Therefore, aflatoxins have little influence on biochemical parameters in the species evaluated. However, exposure to aflatoxins caused liver changes, such as cell death, fatty and hydropic degeneration, thus it could be concluded that both species are susceptible to the toxic effects of long-term exposure to dietary AFB1.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41774337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxin B1 contamination of feedstuff on a dairy farm in Northern Peru and aflatoxin M1 concentrations in raw milk","authors":"I. Salazar, I. López, P. Glorio‐Paulet, C. Gómez","doi":"10.3920/wmj2020.2672","DOIUrl":"https://doi.org/10.3920/wmj2020.2672","url":null,"abstract":"Research regarding aflatoxin contamination levels in Peru is limited, although aflatoxin M1 (AFM1) and aflatoxin B1 (AFB1) require surveillance because of their toxicity. European regulations state that the harmonised maximum level (ML) is 5 μg/kg for AFB1 in feedstuffs and 0.05 μg/kg for AFM1 in milk. Our study aimed to determine the annual variation levels of AFB1 in ingredients used in feedstuffs for dairy cows and those of AFM1 in milk at a typical intensive dairy farm in Northern Peru. For 1 year, milk (n=529) and feedstuff samples (n=235) were collected and aflatoxin levels were determined using a lateral flow immunoassay. We found that 16% of milk samples had AFM1 contamination above the ML. AFM1 level was significantly higher (P<0.05) in December (end of spring) than that in all other months. Throughout the year, the most used feedstuffs were maize, soybean meal and whole soybean. Among the maize samples (n=77), 2.59% had an AFB1 level above the ML, whereas 45% had an AFB1 level below the ML. On the other hand, neither the soybean meal (n=69) nor whole soybean samples (n=64) had an AFB1 level above the ML, 46.4 and 20%, respectively. In 50% (n=10) of cottonseed meal samples, AFB1 level was above the ML; in 20% of wheat middling samples, it was above the ML. Cottonseed and wheat middling samples were used for 2 and 5 months, respectively. AFB1 level in feedstuff showed a significant difference in December (P<0.05) compared with other months, specifically for maize and soybean meal. As the AFM1 level in milk results from AFB1 contaminated feedstuff, our results emphasise the need to implement specific quality measures to reduce contamination.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48506110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}