World Mycotoxin Journal最新文献_第6页

A novel chemical marker of tremorgenic mycotoxicosis detected by gas-chromatography/mass-spectrometry 气相色谱/质谱法检测震颤性真菌毒素新的化学标志物

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-12-13 DOI: 10.3920/wmj2020.2633

C. Pickard, J. Fortin, D. Holmes, J. Buchweitz, A. Lehner

{"title":"A novel chemical marker of tremorgenic mycotoxicosis detected by gas-chromatography/mass-spectrometry","authors":"C. Pickard, J. Fortin, D. Holmes, J. Buchweitz, A. Lehner","doi":"10.3920/wmj2020.2633","DOIUrl":"https://doi.org/10.3920/wmj2020.2633","url":null,"abstract":"Tremorgenic mycotoxicosis can arise from dietary exposure to secondary metabolite products of various fungal species, particularly those from the Penicillium genus. Although general toxin screens often rely on gas chromatography-mass spectrometry (GC/MS) and well-developed mass spectral library databases, two principal representative Penicillium mycotoxins, roquefortine and penitrem A, are unamenable to GC/MS owing to high molecular weights, low volatilities and/or high thermal instabilities. Reliance on GC/MS screens alone could therefore inadvertently result in failure to collect evidence of exposure to such tremorgenic mycotoxins. In this report we describe a newly discovered tremorgenic marker compound (TMC), the presence of which correlates highly with conclusive exposure to Penicillium toxins in explanation of clinical manifestations of tremorgenic mycotoxicosis. According to detailed mass spectral deconvolution, the compound is 210.0892 molecular weight, and amenable to GC/MS whether chemically underivatized or derivatized by methylation or trimethylsilylation. 1D and 2D NMR (nuclear magnetic resonance) studies on the isolated compound determined the TMC to be the Penicillium product terrestric acid, C11H14O4, which matches the molecular formula determined by high resolution mass spectrometry and thus provides an excellent target for assessment of mycotoxicosis by GC/MS.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49150706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibition of Aspergillus flavus growth and aflatoxin B1 production by natamycin 纳他霉素对黄曲霉生长和黄曲霉毒素B1产生的抑制作用

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-12-02 DOI: 10.3920/wmj2020.2620

P. Chang, B. Tai, M. Zheng, Qing Yang, F. Xing

{"title":"Inhibition of Aspergillus flavus growth and aflatoxin B1 production by natamycin","authors":"P. Chang, B. Tai, M. Zheng, Qing Yang, F. Xing","doi":"10.3920/wmj2020.2620","DOIUrl":"https://doi.org/10.3920/wmj2020.2620","url":null,"abstract":"Aspergillus flavus causes huge crop losses, reduces crop quality and has adverse effects on human and animal health. A large amount of food contaminated with aflatoxin can greatly increase the risk of liver cancer. Therefore, prevention and control of aflatoxin production have aroused attention of research in various countries. Natamycin extracted from Streptomyces spp. has been widely used in production practice due to its good specificity and safety. Here, we found that natamycin could significantly inhibit fungal growth, conidia germination, ergosterol and AFB1 production by A. flavus in a dose-dependent manner. Scanning electron microscope analysis indicated that the number of conidia was decreased, the outer wall of conidia was destroyed, and the mycelia were shrivelled and tangled by natamycin. RNA-Seq data indicated that natamycin inhibited fungal growth and conidia development of A. flavus by significantly down-regulating some genes involved in ergosterol biosynthesis, such as Erg13, HMG1 and HMG2. It inhibited conidia germination by significantly down-regulating some genes related to conidia development, such as FluG and VosA. After natamycin exposure, the decreased ratio of aflS/aflR caused by the down-regulation of all the structural genes, which subsequently resulted in the suppression of AFB1 production. In conclusion, this study served to reveal the inhibitory mechanisms of natamycin on fungal growth and AFB1 biosynthesis in A. flavus and to provide solid evidence for its application in controlling AFB1 contamination.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41823971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of aflatoxin B1 and monensin interaction on liver and intestine of poultry – influence of a biological additive (Pichia kudriavzevii RC001) 黄曲霉毒素B1和莫能菌素相互作用对家禽肝脏和肠道的影响——生物添加剂（毕赤酵母RC001）的影响

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-12-02 DOI: 10.3920/wmj2021.2692

A. Magnoli, V. Poloni, L.A. Cristofolini, C. Merkis, F. Escobar, C. Torres, S. Chiacchiera, L. Cavaglieri

{"title":"Effects of aflatoxin B1 and monensin interaction on liver and intestine of poultry – influence of a biological additive (Pichia kudriavzevii RC001)","authors":"A. Magnoli, V. Poloni, L.A. Cristofolini, C. Merkis, F. Escobar, C. Torres, S. Chiacchiera, L. Cavaglieri","doi":"10.3920/wmj2021.2692","DOIUrl":"https://doi.org/10.3920/wmj2021.2692","url":null,"abstract":"The aim of this study was to evaluate the effects of aflatoxin B1 (AFB1) and monensin (MONS) interaction on the liver and intestinal histological changes in poultry, and the influence of Pichia kudriavzevii RC001. One-day-old commercial line (Ross 308) broilers (n=120) were individually weighed and randomly assigned to 8 treatments (15 broilers/treatment, 5 broilers per cage and 3 replicates/treatment). The experimental diets were: Group 1: basal diet (BD); Group 2: BD + MONS (50 mg/kg); Group 3: BD + P. kudriavzevii RC001 (1 g/kg); Group 4: BD + AFB1 (100 μg/kg); Group 5: BD + MONS + P. kudriavzevii RC001; Group 6: BD + AFB1 + P. kudriavzevii RC001; Group 7: BD + AFB1 + MONS + P. kudriavzevii RC001; Group 8: BD + AFB1 + MONS. When MONS was added, the typical AFB1 macroscopic and microscopic alterations were intensified. The P. kudriavzevii RC001 cytotoxicity and genotoxicity assays with Vero cells and with broiler chicken’s erythrocytes, demonstrated that P. kudriavzevii RC001 neither were non-cytotoxic nor genotoxic. When MONS was added in the presence of P. kudriavzevii RC001, the toxic effect of AFB1 on liver was not prevented. When P. kudriavzevii was present alone, the same prevention of the pathological damage was observed in the intestine of poultry fed with AFB1. The smallest apparent absorption area was obtained when AFB1 and MONS were added in the feed (P<0.05). AFB1 and MONS interaction demonstrated important toxic effects. Although P. kudriavzevii was effective in ameliorating the adverse effects of AFB1 alone on liver pathology and gut morphology, it was not able to diminish the toxic effects of AFB1 in presence of MONS. It suggests that P. kudriavzevii could be used as feed additive or counteracting the toxic effects of AFB1 in poultry production in the absence of MONS.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43155273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abiotic factors affect growth and aflatoxin B1 production by Aspergillus flavus strains on chilli powder and red chillies 非生物因素影响黄曲霉菌株在辣椒粉和红辣椒上的生长和黄曲霉毒素B1的产生

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-11-26 DOI: 10.3920/wmj2021.2715

D. Al-Jaza, Á. Medina, N. Magan

{"title":"Abiotic factors affect growth and aflatoxin B1 production by Aspergillus flavus strains on chilli powder and red chillies","authors":"D. Al-Jaza, Á. Medina, N. Magan","doi":"10.3920/wmj2021.2715","DOIUrl":"https://doi.org/10.3920/wmj2021.2715","url":null,"abstract":"Chillies and chilli-based products are important spices on a global basis. The production, processing, transport and storage phases of chillies are prone to infection by Aspergillus Section Flavi and contamination with aflatoxins (AFs), especially aflatoxin B1 (AFB1) for which legislative limits exist in many countries. We have examined the effect of the interacting abiotic factors of water availability (water activity, aw; 0.995-0.850 aw) and temperature (15-37 °C) on (a) lag phases prior to growth, (b) growth, (c) AFB1 production and (d) contour maps of optimum and boundary conditions for colonisation and toxin production by three Aspergillus flavus strains on a 10% chilli-based medium. Additional studies with whole red chillies + A. flavus conidial inoculum on AFB1 contamination during storage for 10-20 days at 30 °C were also carried out. In vitro, the lag phases before growth were delayed by lower temperatures (15, 20 °C) and aw levels (0.928-0.901 aw). There was no statistical difference in growth between the three strains. Optimal growth was at 37 °C and 0.982 aw with no growth at 0.85 aw. Optimal temperature × aw conditions for AFB1 production were at 30 °C and 0.982 aw with no statistical difference in production between strains. No AFB1 was produced at 15-20 °C at 0.901 and 0.928 aw levels, respectively. In situ studies with A. flavus inoculated whole red chillies at 0.90 and 0.95 aw found that this species became the major component of the total fungal populations at 30 °C after 10-20 days storage. AFB1 contamination was above the European legislative limits (5 μg/kg) for spices at 0.90 aw after 20 days storage and at 0.95 aw after 10 and 20 days. This suggests that storage conditions of ≥0.90 aw, especially at ≥25-30 °C represents a significant risk of contamination with AFB1 at levels where rejection might occur, even after only 10-20 days storage.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45149347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Review of good agricultural practices for smallholder maize farmers to minimise aflatoxin contamination 审查玉米小农的良好农业规范，以尽量减少黄曲霉毒素污染

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-11-03 DOI: 10.3920/wmj2021.2685

F. Xu, R. Baker, T. Whitaker, H. Luo, Y. Zhao, A. Stevenson, C. Boesch, G. Zhang

{"title":"Review of good agricultural practices for smallholder maize farmers to minimise aflatoxin contamination","authors":"F. Xu, R. Baker, T. Whitaker, H. Luo, Y. Zhao, A. Stevenson, C. Boesch, G. Zhang","doi":"10.3920/wmj2021.2685","DOIUrl":"https://doi.org/10.3920/wmj2021.2685","url":null,"abstract":"Maize is consumed world-wide as staple food, livestock feed, and industrial raw material. However, it is susceptible to fungal attack and at risk of aflatoxin contamination under certain conditions. Such contamination is a serious threat to human and animal health. Ensuring that the maize used by food industry meets standards for aflatoxin levels requires significant investment across the supply chain. Good Agricultural Practices (GAP) form a critical part of a broader, integrated strategy for reduction of aflatoxin contamination. We reviewed and summarised the GAP of maize that would be effective and practicable for aflatoxin control within high-risk regions for smallholder farmers. The suggested practicable GAP for smallholder farmers were: use of drought-tolerant varieties; timely harvesting before physiological maturity; sorting to remove damaged ears and those having poor husk covering; drying properly to 13% moisture content; storage in suitable conditions to keep the crop clean and under condition with minimally proper aeration, or ideally under hermetic conditions. This information is intended to provide guidance for maize growers that will help reduce aflatoxin in high-risk regions, with a specific focus on smallholder farmers. Following the proposed guidelines would contribute to the reduction of aflatoxin contamination during pre-harvest, harvest, and post-harvest stages of the maize value chain.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42400336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

An update on genotoxic and epigenetic studies of fumonisin B1 伏马菌素B1基因毒性和表观遗传学研究进展

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-11-03 DOI: 10.3920/wmj2021.2720

I. B. Molina-Pintor, A. Rojas-García, I. Medina-Díaz, B. Barrón-Vivanco, Y. Y. Bernal-Hernández, L. Ortega-Cervantes, A. Ramos, J. F. Herrera-Moreno, C. A. González-Arias

{"title":"An update on genotoxic and epigenetic studies of fumonisin B1","authors":"I. B. Molina-Pintor, A. Rojas-García, I. Medina-Díaz, B. Barrón-Vivanco, Y. Y. Bernal-Hernández, L. Ortega-Cervantes, A. Ramos, J. F. Herrera-Moreno, C. A. González-Arias","doi":"10.3920/wmj2021.2720","DOIUrl":"https://doi.org/10.3920/wmj2021.2720","url":null,"abstract":"Fumonisins (FBs), a widespread group of mycotoxins produced by Fusarium spp., are natural contaminants in cereals and foodstuffs. Fumonisin B1 (FB1) is the most toxic and prevalent mycotoxin of this group, and it has been reported that FB1 accounts for 70-80% of FBs produced by the mycotoxigenic strains. The mode of action of FB1 depends on the structural similarity with sphinganine/sphingosine N-acyltransferase. This fact causes an accumulation of sphingoid bases and blocks the sphingolipid biosynthesis or the function of sphingolipids. Diverse toxic effects and diseases such as hepatocarcinogenicity, hepatotoxicity, nephrotoxicity, and cytotoxicity have been reported, and diseases like leukoencephalomalacia in horses and pulmonary oedema in horses and swine have been described. In humans, FBs have been associated with oesophageal cancer, liver cancer, neural tube defects, and infantile growth delay. However, despite the International Agency for Research on Cancer designated FB1 as a possibly carcinogenic to humans, its genotoxicity and epigenetic properties have not been clearly elucidated. This review aims to summarise the progress in research about the genotoxic and epigenetics effects of FB1.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41810304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Association of exposure to deoxynivalenol with DNA methylation in white blood cells in children in China 中国儿童接触脱氧雪腐镰刀菌烯醇与白细胞DNA甲基化的关系

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-10-22 DOI: 10.3920/wmj2021.2699

P. Cao, X.D. Wang, J. Sun, J. Liang, P. Zhou, H. Xu, H. Yang, L. Zhang

引用次数: 0

Interlaboratory collaboration to determine the performance of the Randox food diagnostics biochip array technology for the simultaneous quantitative detection of seven mycotoxins in feed 实验室间合作，确定Randox食品诊断生物芯片阵列技术同时定量检测饲料中七种真菌毒素的性能

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-10-22 DOI: 10.3920/wmj2021.2696

L. Sibanda, K. McCallum, M. Plotan, S. Webb, B. Snodgras, Q. Muenks, J. Porter, P. Fitzgerald

{"title":"Interlaboratory collaboration to determine the performance of the Randox food diagnostics biochip array technology for the simultaneous quantitative detection of seven mycotoxins in feed","authors":"L. Sibanda, K. McCallum, M. Plotan, S. Webb, B. Snodgras, Q. Muenks, J. Porter, P. Fitzgerald","doi":"10.3920/wmj2021.2696","DOIUrl":"https://doi.org/10.3920/wmj2021.2696","url":null,"abstract":"An inter-laboratory collaborative study was performed to evaluate the performance of the Biochip Array Technology (BAT) Myco 7 method. The Myco 7 Array is a method which simultaneously and quantitatively detects 20 mycotoxins including aflatoxins B1, B2, G1 and G2, ochratoxin A, deoxynivalenol, zearalenone, fumonisin B1, B2 and B3 and T-2 and HT-2 toxin. The BAT Myco 7 method was collaboratively evaluated by nine government and private Association of American Feed Control Officials (AAFCO) laboratories. Samples were analysed in a proficiency testing round format. Seventeen blind samples were analysed on the same equipment using Myco 7 kits. 99% of the results fell within an acceptable Z-score range of -2|<Z<|+2. Deoxynivalenol had a 100% Z-score pass rate, while a 99% pass was recorded for aflatoxins, zearalenone, ochratoxin A and fumonisins. T-2 toxin had a 97% Z-score pass rate. HorRat analysis for reproducibility used a range of 0.3<|HorRat|≤2. The target was met for deoxynivalenol, zearalenone, T-2 and HT-2 toxin, and aflatoxins B1, B2, G1 and G2 assays. Fumonisins and ochratoxin A assays had a 93% and 94% pass, respectively. The reproducibility co-efficiency of variation was between 16 and 20% meeting set criterion of <40% and is, therefore, fit-for-purpose for use in the AAFCO control programs for mycotoxins.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46133264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Foreword – special issue Mycotoxins in Latin America 前言-拉丁美洲真菌毒素特刊

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-09-14 DOI: 10.3920/wmj2021.x003

{"title":"Foreword – special issue Mycotoxins in Latin America","authors":"","doi":"10.3920/wmj2021.x003","DOIUrl":"https://doi.org/10.3920/wmj2021.x003","url":null,"abstract":"Latin America with its considerable North-South extent is subject to climate that varies from tropical, subtropical and warm temperate to temperate. Different agricultural products are produced in the area including cereals, oilseeds, beans, fruits and nuts together with animal production including cattle for beef and milk, pigs, poultry and fish. The heterogeneity of agriculture in Latin America is reflected in the diversity of the region’s farm structures. While agriculture in the Southern Cone is dominated by large, commercial and export-oriented farms, particularly in Argentina and Brazil, besides increasingly in other countries like Uruguay, much of the rest of the region is characterised by smallholder and family agriculture. The contamination of agricultural products with mycotoxins has impact both human and animal health, as well as the economy due to losses related to rejections of agricultural products and by-products during trade. The economic burden related to the consumption of mycotoxins by animals is especially important, causing productivity losses up to the death of animals. The relevant mycotoxins are fumonisins, deoxynivalenol (DON) and zearalenone (ZEN) in cereals and cereal-based products, aflatoxins in cereals, oily seeds and nuts, aflatoxin M1 in milk and dairy products as well as ochratoxin A (OTA) in coffee, grapes and raisins. Co-occurrence of mycotoxins has also been observed mainly with aflatoxins and fumonisins in different Latin American countries (Torres et al., 2015). Advances on legislation in different countries including Argentina, Brazil, Chile, Mexico and Uruguay have been done to establish maximum limits for mycotoxins including aflatoxins, DON, ZEN, OTA, patulin and ergot alkaloids (ANVISA, 2011/2017; CAA, 2019/2021, Norma Oficial Mexicana, N.-243-S., 2010/2010; Reglamento Sanitario de los Alimentos, 2013).","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42212814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smartphone-based technology for nanomolecular detection of aflatoxin B1 by aptamer-conjugated magnetic nanoparticles 基于智能手机的配体共轭磁性纳米颗粒黄曲霉毒素B1纳米分子检测技术

IF 2 4区医学

World Mycotoxin Journal Pub Date : 2021-09-13 DOI: 10.3920/wmj2021.2702

A. Rafati, N. Dorosti, P. Gill

{"title":"Smartphone-based technology for nanomolecular detection of aflatoxin B1 by aptamer-conjugated magnetic nanoparticles","authors":"A. Rafati, N. Dorosti, P. Gill","doi":"10.3920/wmj2021.2702","DOIUrl":"https://doi.org/10.3920/wmj2021.2702","url":null,"abstract":"The need for a healthy market in the rapid and accurate screening of a variety of pathogenic agents and toxins in the environment and food has led to an increase in the development of new biosensors, which have ideal characteristics, such as high sensitivity and specificity with rapid detection and simple preparation of the sample. Among the food contaminants, mycotoxins have been identified as a major challenge for the food industry, and rapid and accurate detection has attracted the attention of food inspection and monitoring organisations. In this study, a nanomolecular detection method is described using aflatoxin B1 (AFB1)-specific aptamers attached to streptavidin-coated magnetic nanoparticles. A prominent feature of the AFB1-specific aptamers is a guanine-rich (G-rich) sequence with a G-quadruplex structure after capturing AFB1 molecules and mimicking peroxidase activity. The enzymatic reaction evaluated in the presence of chromogenic substrate and measurement is done by a smartphone-specific application for colorimetric measurement. The results indicated that the assay could measure AFB1 in rice, flour, seed, maize, and pistachio. In addition, the application of hybrid nanomaterial technology resulting from the binding of biotin-labelled aptamers to the surface of streptavidin-coated magnetic nanoparticles minimises preparation and treatment of samples, improves results, and consequently reduces false negative and positive responses in the detection field. This study may eventually lead to the design and development of a fast, sensitive, specific, and on-site AFB1-based nanomolecular colorimetric detection system via a smartphone-based application that can be readily accessible to all applicants, from professionals to manufacturers of foodstuffs.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48032953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0