Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B最新文献_第7页

Detection of Vibrio anguillarum by a sandwich enzyme-linked immunosorbent assay performed with monoclonal antibodies. 单克隆抗体夹心酶联免疫吸附法检测鳗弧菌。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00356.x

R Adone, G Piccininno, C Pistoia, R Bianchi, F Ciuchini

引用次数: 4

Evaluation of polymerase chain reaction (PCR) application in diagnosis of bovine leukaemia virus (BLV) infection in naturally infected cattle. 聚合酶链反应(PCR)在牛白血病病毒(BLV)自然感染诊断中的应用评价。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00361.x

H Fechner, A Kurg, L Geue, P Blankenstein, G Mewes, D Ebner, D Beier

{"title":"Evaluation of polymerase chain reaction (PCR) application in diagnosis of bovine leukaemia virus (BLV) infection in naturally infected cattle.","authors":"H Fechner, A Kurg, L Geue, P Blankenstein, G Mewes, D Ebner, D Beier","doi":"10.1111/j.1439-0450.1996.tb00361.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00361.x","url":null,"abstract":"The practical application of polymerase chain reaction (PCR) for the diagnosis of bovine leukaemia virus (BLV) infections in naturally infected cattle was evaluated. Compared to serological tests the PCR was definitely found to be a more sensitive method, yielding the highest number of positive results (10% more compared to enzyme-linked immunosorbent assay, (ELISA), and 17.7% more compared to agar-gel immunodiffusion, (AGID)). In testing cattle from herds with BLV incidence under 5%, out of 52 provirus positive cattle only 43 were correctly identified by ELISA. When compared to AGID only 37 of the 52 PCR positive animals were correctly identified. Of 18 cattle imported from the Slovak Republic and kept in a quarantine stable, four were found to be BLV provirus positive by PCR, while serological tests indicated one animal positive and three negative. Therefore, it is impossible to prevent the spread of the infection from one country to another by serological testing only. Moreover, it is feasible to identify animals with changing antibody titres correctly by PCR. Using PCR we were also able to distinguish BLV infected from uninfected calves that were serologically positive due to colostral antibodies. Higher sensitivity of BLV provirus detection by PCR was achieved using env gene rather than tax gene specific primers. Negative results by PCR in cases of positive serological reactions are still possible, as shown in case of one adult animal. These findings indicate that PCR is a highly sensitive method and might be successfully used and economically advantageous for different practical applications in detection of BLV infection in naturally infected cattle.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 10","pages":"621-30"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00361.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19970823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 65

Immunohistological study of IgA, IgG and IgM in endoscopic biopsies of dogs with plasmacytic-lymphocytic colitis. 浆细胞-淋巴细胞性结肠炎犬内镜活检中IgA、IgG和IgM的免疫组织学研究。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00360.x

I Mayoral, L Peña, F Rodriguez-Franco, A Sainz, M A Tesouro, E Ynaraja

引用次数: 6

An outbreak of Pacheco's parrot disease in psittacine birds recently imported to Campania, Italy: isolation of psittacid herpesvirus 2. 最近在意大利坎帕尼亚输入的鹦鹉中爆发帕切科鹦鹉病:分离出鹦鹉疱疹病毒2型。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00362.x

S Magnino, G Conzo, A Fioretti, L F Menna, T Rampin, G Sironi, M Fabbi, E F Kaleta

引用次数: 12

Environment, incidence, aetiology, epizootiology and immunoprophylaxis of soil-borne diseases in north-east Mexico. 墨西哥东北部土壤传播疾病的环境、发病率、病原学、流行病学和免疫预防。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00358.x

H S Seifert, K Bader, J Cyplik, J González Salinas, F Roth, J A Salinas Meléndez, U Sukop

{"title":"Environment, incidence, aetiology, epizootiology and immunoprophylaxis of soil-borne diseases in north-east Mexico.","authors":"H S Seifert, K Bader, J Cyplik, J González Salinas, F Roth, J A Salinas Meléndez, U Sukop","doi":"10.1111/j.1439-0450.1996.tb00358.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00358.x","url":null,"abstract":"Within the framework of an extensive research programme, the socio-economic and environmental conditions which influence the emergence of soil-borne diseases in north-eastern Mexico were analysed. Furthermore, specimens collected from carcasses in the field were bacteriologically examined and the causal organisms of soil-borne diseases differentiated by means of gas chromatographic analysis of their metabolic products and the long-chained fatty acids contained in the cell. With experimental clostridial vaccines prepared with the Goettingen Bioreactor Technique, trials to protect cattle and guinea-pigs against gas gangrene were carried out. It was found that the farm structure and the dry climate as well as the specific soil conditions and plant cover favour the emergence of soil-borne diseases. Causal organisms B. anthracis, C. perfringens, C. sordellii, C. haemolyticum, C. chauvoei/septicum, C. novyi A, C. botulinum and site-specific field strains of clostridia were detected. Experimental site-specific vaccines proved to be highly efficient in protecting cattle and guinea pigs.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 10","pages":"593-605"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00358.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19970820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Differentiation of Pasteurella multocida subspecies multocida isolates from the respiratory system of pigs by using polymerase chain reaction fingerprinting technique. 聚合酶链反应指纹图谱技术鉴别猪呼吸系统多杀性巴氏杆菌亚种多杀性分离株。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00357.x

B Zucker, M Krüger, F Horsch

引用次数: 12

Immunoreactive cytokines within primates. 灵长类动物体内的免疫反应细胞因子。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI: 10.1111/j.1439-0450.1996.tb00359.x

W Ahne, A Mayr, H Wiesner

引用次数: 4

Detection and genomic analysis of canine parvovirus by the polymerase chain reaction. 犬细小病毒的聚合酶链反应检测及基因组分析。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-11-01 DOI: 10.1111/j.1439-0450.1996.tb00352.x

T Hirasawa, K Yono, K Mikazuki

引用次数: 17

Diagnostic results in animal dermatophytoses. 动物皮癣的诊断结果。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-11-01 DOI: 10.1111/j.1439-0450.1996.tb00351.x

A Schmidt

{"title":"Diagnostic results in animal dermatophytoses.","authors":"A Schmidt","doi":"10.1111/j.1439-0450.1996.tb00351.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00351.x","url":null,"abstract":"Superficial mycoses caused by dermatophytes, as well as asymptomatic carriership of dermatophytes, have a high prevalence among domestic animals and pets. We examined 606 clinical specimens from skin lesions of animals with a significant tendency towards superficial mycosis due to their clinical features. Samples were obtained from horses, dogs, cats, small rodents, birds, and rabbits. The specimens were examined by microscopic and cultural techniques. Microscopically, there was no significant difference in the prevalence of structures which may develop fungal elements between the groups culturally positive or negative for dermatophytes and yeasts. Overall, 24.6% of the samples were microscopically positive. In specimens obtained from horses, a high contamination rate of 36%, mostly due to moulds, was found with a cycloheximide supplemented medium, making the examination of these cultures for the growth of dermatophytes impossible. The other animals showed a significantly lower contamination rate, 11% on average. In horses, Trichophyton equinum had the highest prevalence, in small rodents. Trichophyton mentagrophytes, and in cats Microsporum canis. Overall, 10% of the culturally examinable samples were positive for dermatophytal or yeast growth, though yeasts had only a very low isolation frequency.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 9","pages":"539-43"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00351.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19938293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

[The evaluation of tumor necrosis factor-alpha concentration in body fluids in local and systemic endotoxin exposure]. [局部和全身内毒素暴露时体液肿瘤坏死因子- α浓度的评价]。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-11-01

C Kretzschmar

引用次数: 0