Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B最新文献_第6页

Clinical and microbiological study of an otitis media outbreak in calves in a dairy herd. 奶牛群小牛中耳炎爆发的临床和微生物学研究。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1999-04-01 DOI: 10.1046/j.1439-0450.1999.00187.x

I Yeruham, D Elad, M Liberboim

{"title":"Clinical and microbiological study of an otitis media outbreak in calves in a dairy herd.","authors":"I Yeruham, D Elad, M Liberboim","doi":"10.1046/j.1439-0450.1999.00187.x","DOIUrl":"https://doi.org/10.1046/j.1439-0450.1999.00187.x","url":null,"abstract":"On a dairy farm, otitis media was diagnosed in 64 suckler calves (21.8%) during a study period of 2 years, and in 10 calves (3.4%) in the third year. The inflammation was unilateral in 63 and bilateral in 11 calves. The affected calves were dull, lacked appetite, were pyrexic and displayed drooping ear or ears and tilted heads with purulent discharge exuding from the external ear canal. Of the affected animals, 56 (87.5%) were aged between 3 and 8 weeks. Morbidity was higher during the calving season and during the autumn and winter months (October-December). Pasteurella haemolytica was isolated from 21 (32.8%), P. multocida from 20 (31.2%), Actinomyces pyogenes from 11 (17.2%) and Streptococcus pneumoniae from three (4.7%) of the clinically affected calves only during the first two study years. The exudate of the acute ear infections contained, in addition to Pasteurella spp., various bacteria and yeasts. Most of these bacteria were isolated from healthy ears as well, and are likely to be part of the normal ear flora. On the other hand most of the yeasts were isolated from otitic calves. After a short course of an appropriate treatment infections healed in all cases. Possible preventive measures are discussed.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 3","pages":"145-50"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1439-0450.1999.00187.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21205965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

An evaluation of immunofluorescence and PCR methods for detection of rabies in archival Carnoy-fixed, paraffin-embedded brain tissue. 免疫荧光和PCR检测存档石蜡包埋脑组织狂犬病的方法评价。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1999-04-01 DOI: 10.1046/j.1439-0450.1999.00200.x

K Kulonen, M Fekadu, S Whitfield, C K Warner

{"title":"An evaluation of immunofluorescence and PCR methods for detection of rabies in archival Carnoy-fixed, paraffin-embedded brain tissue.","authors":"K Kulonen, M Fekadu, S Whitfield, C K Warner","doi":"10.1046/j.1439-0450.1999.00200.x","DOIUrl":"https://doi.org/10.1046/j.1439-0450.1999.00200.x","url":null,"abstract":"Direct immunofluorescence and PCR detection methods were compared for sensitivity in evaluating the rabies status of archival specimens of Carnoy-fixed, paraffin-embedded brain tissue. The material consisted of 23 samples obtained during a rabies outbreak in Finland in 1988, and one sample isolated from a bat researcher who died of rabies in Finland in 1985. These results were compared with the original diagnoses performed on the fresh tissues. The immunofluorescence assay detected 100% (12/12) of the rabies-positive archival cases. A PCR assay designed to detect a 139-bp target near the 5' end of the rabies nucleoprotein gene also detected 100% (12/12) of the samples identified as positive in the fresh tissue specimens. A PCR assay designed to detect a 304-bp target spanning the 139-bp target of the first assay detected only 67% (8/12) of the original cases. No false positives were recorded. Both immunofluorescence detection of antigen and PCR detection of a short region of the nucleoprotein gene are useful in determining the rabies status of fixed, paraffin embedded (archival) material.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 3","pages":"151-5"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1439-0450.1999.00200.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21205966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Serological survey of viral antibodies in llamas (Lama glama) in Argentina. 阿根廷大羊驼病毒抗体血清学调查。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1999-04-01 DOI: 10.1046/j.1439-0450.1999.00215.x

M Puntel, N A Fondevila, J Blanco Viera, V K O'Donnell, J F Marcovecchio, B J Carrillo, A A Schudel

引用次数: 78

[Model investigations of the impedance effectiveness conerning bacterial relevant to food hygiene]. [食品卫生相关细菌阻抗有效性的模型研究]。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1998-11-01

J Schulenburg, T Bergann

{"title":"[Model investigations of the impedance effectiveness conerning bacterial relevant to food hygiene].","authors":"J Schulenburg, T Bergann","doi":"","DOIUrl":"","url":null,"abstract":"The impedance technique mostly meets today's requirements of microbiological rapid methods. At relatively high prime cost for the equipment the advantages are marked by low personnel and material costs as well as swiftness combined with highly flexible usage. The method is applicable for both quantitative and qualitative examinations but can fail occasionally in total count determination, especially if the sample material contains heterogeneous microbes. In model investigations with 53 strains of 17 different genera Enterobacteriaceae strains, Aeromonads and Enterococcus strains proved to be highly impedance effective. Lactobacillus strains and Pseudomonads as well as Staphylococcus aureus strains showed a low impedance effectiveness. Several strains, for example of the genera Micrococcus, Acinetobacter and Brochothrix, did not show any changes of the medium impedance under the chosen conditions. Criterion for characterization of impedance effectiveness was the impedance detection time starting with identical initial counts (10(3) cfu/ml). Impedance effectiveness of microbes was determined at highly varying degree by the parameters of generation time, lag-phase duration and relative activity. This can lead either to wrong negative (underestimations) or wrong positive (overestimations) results of bacterial count.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"45 9","pages":"551-9"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Migration and transposition of Oesophagostomum quadrispinulatum larvae from feces]. [四足足食道口幼虫从粪便中迁移和转位]。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1998-05-01

D Barutzki, R Gothe

引用次数: 0

[Presence of Listeria monocytogenes and Listeria sp. in fresh and cured sausages. Use of different conditions of time and temperature for incubation]. [在新鲜及腌制香肠中发现单核增生李斯特菌及李斯特菌属。]利用不同条件的时间和温度进行孵育。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1997-12-01

C Rota, J Yangüela, D Blanco, J J Carramiñana, A Herrera

引用次数: 0

[Comparative investigations of practice-oriented methods for the detection of viruses in food with Aujeszky infection in swine as an example]. [以猪奥耶斯基病毒感染为例的食品病毒实用化检测方法的比较研究]。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1997-11-01

A Mayr

{"title":"[Comparative investigations of practice-oriented methods for the detection of viruses in food with Aujeszky infection in swine as an example].","authors":"A Mayr","doi":"","DOIUrl":"","url":null,"abstract":"In order to detect contamination in foodstuffs originating from animals, three different diagnostic methods were tested in comparison: cultivation in permissive cell cultures, microparticle antigen capture system per FACS (MAS) and polymerase chain reaction (PCR). Assessment implied relevance for health, sensitivity and specificity. Aujeszky infection in swine served as a model. The blood and organs of healthy, but persistently infected as well as specifically diseased animals were examined. In addition, artificially Aujeszky-contaminated milk, black pudding and minced meat were included in the comparison of methods. Basically, all three methods of detecting contamination in raw foodstuffs originating from animals were useful. The virus detection in cell cultures as well as the efficacy of MAS were inhibited by meat products according to their preparation (e.g., virus protein denaturation). PCR turned out to be the only reliable method to confirm the contamination in foodstuffs. Using PCR, viral contamination in foodstuffs originating from healthy but persistently infected animals could be detected. Each of the three virus detection systems has various advantages and disadvantages. They are listed and discussed in detail. With regard to the relevance of health, virus detection in raw meat via cell culture remains the preferred method. Besides the detection of an individual virus, routine examinations of foodstuffs for unknown zoonotic virus contamination, sets based on permissive cell cultures, primer sets for the PCR as well as sets based on various monoclonal antibodies for MAS have to be developed and made available at the diagnostic laboratories.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"44 9","pages":"565-76"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20381123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The effect of optical radiation on the migration of third stage larvae of Oesophagostomum quadrispinulatum out of feces]. [光辐射对四足食道口第三期幼虫从粪便中迁移的影响]。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1997-09-01

D Barutzki, R Gothe

引用次数: 0

[A new Bordetella species in sheep?]. [绵羊中的博德特拉菌新种?]。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1997-06-01

M Mitsching, J Hönig, M Krüger

引用次数: 0

[Evaluation of methods for preparing chicken feces from the veterinary hygienic aspect]. 从兽医卫生角度评价鸡粪制备方法。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1997-04-01

W Dorn, G Schleiff

{"title":"[Evaluation of methods for preparing chicken feces from the veterinary hygienic aspect].","authors":"W Dorn, G Schleiff","doi":"","DOIUrl":"","url":null,"abstract":"Fresh and dried faeces of laying hens from battery fattening and faeces from complete confinement rearing were investigated with bacteriological and physico-chemical methods. The comparison shows that ventilated faeces from conveyor belts with significantly higher values of the autochthonous faecal flora (endogerms, coliform germs, faecal streptococci) are most unfavourable from an epidemiological-bacteriological point of view. Salmonellae occurred very frequently both in fresh faeces (in 76.9% of the samples) and in ventilated faeces from conveyor belts (in 83.9% of the samples), whereas this agent was only detectable in 1.9% of the samples of faeces from complete refinement rearing. Fifteen serovar were isolated, most frequently S. enteritidis (29.4%), but S. typhimurium only once (1.96%). The highest amount of salmonellae germs was found with 105 g in faeces from conveyor belts. There are no objections to the direct utilization of faeces as fertilizers from an epidemiological point of view. For epidemiological reasons, ventilated faeces from conveyor belts should not be directly sprayed over the soil. After air-drying in henhouses, these faeces should be stored and composted before they are used in agriculture. It was not possible to cultivate salmonellae and E. coli in summer and winter after the composting of dried hens' faeces. The salmonellae were no longer detectable from the 4th day onwards, native salmonellae from the 7th day (summer) and the 25th day (winter) onwards, and E. coli from the 88th day onwards. If all parameters, particularly the grain size, are observed, an epidemiologically perfect product comes into being after the fast drying of faeces.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"44 2","pages":"105-18"},"PeriodicalIF":0.0,"publicationDate":"1997-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20136305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0