Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B最新文献

{"title":"[Multiplication, interference, and interferon induction of avian herpesviruses. Contribution to the vaccination against Marek's disease. 2. Materials and methods, results, discussion and conclusion].","authors":"E. Kaleta","doi":"10.1111/J.1439-0450.1977.TB01015.X","DOIUrl":"https://doi.org/10.1111/J.1439-0450.1977.TB01015.X","url":null,"abstract":"Zusammenfassung \u0000 \u0000Die experimentellen Untersuchungsergebnisse beziehen sich auf drei Themenkreise: \u0000 \u0000 \u0000 \u0000a) Vermehrung des Puten-Herpesvirus in vitro: Durch Versuche in der Durchstromungskammer sowie durch Untersuchungen mit markierten Antikorpern konnte der Nachweis erbracht werden, das die Virusweitergabe von Zelle zu Zelle durch erregerhaltige Zytoplasmateile erfolgt, die von infizierten Zellen abgeschnurt und kurze Zeit spater von nicht infizierten Zellen aufgenommen werden. Unter Berucksichtigung dieses offenbar den zellassoziierten Herpesviren der Gruppe B eigenen Ubertragungsweges ist eine grosere Virusproduktion bei gleichzeitiger kurzerer Kultivierungszeit moglich. \u0000 \u0000 \u0000 \u0000b) Interferon: Wahrend drei Puten-Herpesvirusstamme sowie drei apathogene Huhner-Herpesvirusstamme in vitro kein nachweisbares Interferon induzierten, waren drei geprufte pathogene MKV-Stamme in der Lage, sowohl in Huhnerembryofibroblasten- als auch in Huhnerkukennierenzellkulturen die Bildung groser Mengen Interferon zu induzieren. Auf Grund der fehlenden Interferoninduktion durch die drei Puten- und die drei apathogenen Huhner-Herpesvirusstamme kann als sicher gelten, das der Interferonmechanismus nicht fur die Schutzwirkung dieser Stamme gegen das Entstehen der MK in Frage kommt. \u0000 \u0000 \u0000 \u0000c) Interferenz: Es konnten experimentelle Beweise fur das Vorkommen der homologen Interferenz in vitro erbracht werden. Durch Endpunktpassagen mit einem zeitlichen Abstand von 24 Stunden zwischen Infektion und Passage der Kulturen konnte die Bildung inkompletter interferierender Viruspartikel weitgehend vermieden werden. Inaktiviertes Herpesvirus vermag ebenfalls mit infektiosem Virus zu interferieren. \u0000 \u0000 \u0000 \u0000Die Infektion von Huhnerkukennierenzellkulturen mit Puten-Herpesvirus und die nachfolgende Infektion mit pathogenem Marekvirus vermochte die Vermehrung des MKV nachhaltig zu hemmen. Dieses in vitro beobachtete, als heterologe Interferenz bezeichnete Ereignis tritt Literaturberichten zufolge auch in vivo auf und wird als eines der Wirkungsprinzipien der Schutzimpfung von Eintagskuken gegen die MK angesehen. \u0000 \u0000 \u0000 \u0000Summary \u0000 \u0000Multiplication, interference and interferon induction of avian herpesviruses. Protection against Marek's Disease \u0000 \u0000 \u0000 \u00002. Materials and methods, results, discussion and conclusions \u0000 \u0000 \u0000 \u0000The results of experimental studies relate to three different themes. \u0000 \u0000 \u0000 \u0000a) Multiplication of turkey herpesvirus in vitro. Studies using the continuous culture chamber and experiments with labelled antibodies have shown that spread of virus from cell to cell takes place by virus-containing cytoplasmic particles which become detached from infected cells and are taken up by uninfected cells. Consideration of this method of transmission of herpesviruses of Group B suggests that a very large amount of virus can be produced in a very short period of culture. \u0000 \u0000 \u0000 \u0000b) Interferon. Whereas three turkey herpesvirus strains and three nonpathogenic chicken herpesvirus strains pr","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"84 1","pages":"429-75"},"PeriodicalIF":0.0,"publicationDate":"2010-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78719102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Behavior of Newcastle disease virus in the airborne state. 1. Experimental study of particle binding of the virus].","authors":"B. Lutz, K. Koch, W. Müller, D. Strauch","doi":"10.1111/J.1439-0450.1984.TB01309.X","DOIUrl":"https://doi.org/10.1111/J.1439-0450.1984.TB01309.X","url":null,"abstract":"","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"9 1","pages":"321-8"},"PeriodicalIF":0.0,"publicationDate":"2010-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81050754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Separation of serum proteins from various domestic mammals using gel electrophoresis. 1. Contribution to the technic of agar electrophoresis].","authors":"W. Matthaeus","doi":"10.1111/j.1439-0450.1965.tb01378.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1965.tb01378.x","url":null,"abstract":"","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"70 1","pages":"133-42"},"PeriodicalIF":0.0,"publicationDate":"2010-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76664986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental infection by Vibrio anguillarum in mice and guinea pigs.","authors":"F Ciuchini,&nbsp;R Adone,&nbsp;G Piccininno,&nbsp;G Antonucci,&nbsp;C Pistoia,&nbsp;F Mutinelli,&nbsp;G Giorgetti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The fish pathogen Vibrio anguillarum causes a lethal infection in farmed fish characterized by hemorrhagic septicemia. There are no reports of experimental laboratory infections in warm-blooded animals. We investigated the effects of an intraperitoneal infection with different doses of a V. anguillarum suspension in mice and guinea pigs. The infection caused a 95-100% of mortality in 24-48 h. Hemorrhagic septicemia was observed at necropsy and confirmed by histological and hematological examination. Immunohistochemically positive bacterial clumps were detected exclusively in vessel lumen in all examined organs, including brain, and V. anguillarum was reisolated in pure culture from all organs, particularly from the kidney. Blood analysis showed erythropenia and leukopenia with granulocytosis in mice, platelet reduction and leukopenia with lymphocytosis in guinea pigs.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"271-7"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the dot-immunobinding assay with the serum agglutination test, the rose bengal plate test and the milk ring test for the detection of Brucella antibodies in bovine sera and milk.","authors":"K Gürtürk,&nbsp;B Boynukara,&nbsp;Z Ilhan,&nbsp;I Hakki Ekin,&nbsp;T Gülhan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study, Brucella antibodies in bovine sera and milk were detected using the dot-immunobinding assay (DIA), the serum agglutination test (SAT), the Rose Bengal plate test (RBPT) and the milk ring test (MRT). For this purpose, a total of 116 paired blood and milk samples collected at the same time from 56 aborted and from 60 healthy dairy cows was examined. In DIA, a nitrocellulose membrane (NCM) was used as the solid phase. Antigen adsorbed on the NCM was extracted from Brucella abortus S99 by heat treatment. The results obtained by DIA were compared with those of SAT, RBPT and MRT. Of the 116 paired blood and milk samples, 24 were positive and 72 were negative by all tests used. Serum samples of six aborted cows were positive by DIA, SAT and RBPT but the milk samples were negative by DIA and MRT. Serum and milk samples of four aborted cows gave positive reaction only by DIA tests. The remaining six aborted cows were negative only by MRT and two of them were negative by both RBPT and MRT. Four sera of healthy cows were found to be positive only by SAT.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"279-85"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiologic, pathogenic and molecular analysis of recent encephalomyocarditis outbreaks in Belgium.","authors":"F Koenen,&nbsp;H Vanderhallen,&nbsp;F Castryck,&nbsp;C Miry","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In 1991 EMCV was isolated for the first time in Belgium from the offspring of a sow with reproductive failure. From August 1995 until December 1996, EMCV was diagnosed in 154 Belgian pig holdings in association with myocardial failure and sudden death in fatteners and suckling piglets or with reproductive failure in sows. To clarify some epidemiological aspects 3 EMCV isolates characteristic for the different clinical pictures and outbreaks were studied. Field observations and animal experiments indicated that the pathogenicity induced by each isolate is specific for one age category and that the spread of the virus is limited. The presented data also suggest that rodents may play a role in the transmission of EMCV but that pig-to-pig transmission is at least as important. Molecular analysis of two separate regions on the genomes of the respective EMCV isolates showed that the 1995-96 EMCV epizootic in Belgium was due to a new virus introduction. Furthermore, the VP1 coding gene is proposed as a marker of virulence.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"217-31"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental infections with Actinobacillus pleuropneumoniae in pigs--II. Comparison of antibiotics for oral strategic treatment.","authors":"P Wallgren,&nbsp;T Segall,&nbsp;A Pedersen Mörner,&nbsp;A Gunnarsson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study was aimed at scrutinizing the efficacy of oral antimicrobial treatments at experimental challenge using a strain of Actinobacillus pleuropneumoniae serotype 2 known to cause severe disease. SPF pigs aged 10 weeks were infected intranasally and the antimicrobial treatments were initiated 5 h prior to that exposure. Several antimicrobial drugs, as well as the length of the treatment period, were elucidated. The outcome of the challenge was monitored by registration of clinical symptoms, weight gains and the development of serum antibodies to A. pleuropneumoniae. At necropsy, the magnitude of pathological lesions in the respiratory tract and the rate of reisolation of the infective strain were recorded. Animals that became diseased displayed a decreased growth rate caused, to a large extent, by a reduced feed intake. The performance with respect to daily weight gain and feed conversion corresponded well with the clinical signs developed and serologic reactions, as well as with the findings made at necropsy. The results obtained among pigs treated with enrofloxacin, but also with florfenicol or chlortetracycline, were superior to those of pigs treated with penicillin, tiamulin or tilmicosin. A positive effect was obtained using a strategic in-feed medication against infection with A. pleuropneumoniae. Provided that the drug used is effective against the target microbe, initiating treatment prior to infection appeared to be more important than the length of the treatment. It should, however, be remembered that A. pleuropneumoniae was reisolated from all but one medicated group following an experimental challenge given after initiating the medication. Consequently medical treatment as described did not eradicate the microbe.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"261-9"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of buparvaquone treatment on the levels of some antioxidant vitamins, lipid peroxidation and glutathione peroxidase in cattle with theileriosis.","authors":"M Naziroğlu,&nbsp;C E Saki,&nbsp;M Sevgili","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasma levels of vitamins A, E, beta carotene, both plasma and erythrocyte glutathione peroxidase (GSHPx), lipid peroxidation (LPO) and reduced glutathione (GSH) were investigated in cattle naturally infected with Theileria annulata and treated with buparvaquone. There were two groups each containing 30 cattle. Naturally infected cattle were used in the second group. Buparvaquone (2.5 mg/kg body weight) was administered to animals in the second group. Blood samples were taken from control animals, and immediately before treatment, and from animals 10 days after the injection of buparvaquone. Detection of the infected animals was carried out by blood smears. Plasma vitamins A, E, beta carotene, both plasma and erythrocyte GSHPx, LPO and GSH levels were determined. The levels of LPO in plasma and erythrocyte samples were significantly (P < 0.05, P < 0.01) higher after treatment than in either control animals or before treatment. Plasma levels of antioxidant vitamins, vitamin E and beta carotene were significantly (P < 0.05, P < 0.01) lower after treatment than in either control animals or before treatment, while the vitamin E level was found to be higher before treatment than in either the control group or animals after treatment (P < 0.05, P < 0.01). The levels of vitamin A in plasma and the activity of GSHPx and GSH in both plasma and erythrocytes in control animals after and before treatment did not differ significantly. In conclusion, we observed that there was a decreased plasma level of vitamin E and beta carotene and an increased level of LPO in cattle treated with buparvaquone. Buparvaquone might function in the treatment of Theileria annulata by forming free radicals.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"233-9"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotypes of Pasteurella haemolytica and Pasteurella trehalosi isolated from farm animals in Hungary.","authors":"L Fodor,&nbsp;J Varga,&nbsp;I Hajtós,&nbsp;T Molnár","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The biochemical and serological characteristics of 486 P. haemolytica and 31 P. trehalosi strains (517 in total) isolated from different lesions of cattle, sheep, goats, pigs and poultry were examined. A total of 476 P. haemolytica strains (97.9%) showed the characteristics typical of the former biotype A of P. haemolytica, while 10 isolates (2.1%), all from poultry, could not be biotyped. A total of 481 strains (93.0%) could be assigned to one of the 17 serotypes of P. haemolytica-P. trehalosi and 36 strains (7.0%) could not. The majority (83.6%) of the cattle isolates were serotypes A1 and A2. Among strains isolated from sheep all serotypes of P. haemolytica could be identified with the exception of A14, but serotypes A1, A2, A6, A8 and A5 were the most frequent. The overwhelming majority (94%) of the caprine isolates were A2, other serotypes occurred only sporadically. The pig isolates, which could be isolated only very rarely, represented different serotypes, while none of the 10 strains isolated from poultry could be biotyped or serotyped.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"241-7"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental infections with Actinobacillus pleuropneumoniae in pigs--I. Comparison of five different parenteral antibiotic treatments.","authors":"P Wallgren,&nbsp;T Segall,&nbsp;A Pedersen Mörner,&nbsp;A Gunnarsson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>SPF pigs aged 10 weeks were infected intranasally with Actinobacillus pleuropneumoniae serotype 2. After the onset of clinical symptoms of respiratory disease, which occurred 20 h post-infection, parenteral treatment with ceftiofur, danofloxacin, enrofloxacin, penicillin or tiamulin was initiated (n = 8 per group). Untreated groups, of which one was infected, served as controls. The uninfected control group did not show any signs of disease, while the infected control group was severely affected by the infection and also expressed a decreased weight gain following the challenge. Based on clinical signs, the magnitude of pathological lesions in the respiratory tract found at necropsy performed 17 days post-infection and the number of reisolates of A. pleuropneumoniae made at necropsy, treatments with the quinolones (danofloxacin and enrofloxacin) and the cephalosporine (ceftiofur) were superior to those with penicillin and tiamulin. The latter groups also developed antibodies to A. pleuropneumoniae to a larger extent. Some of the pigs treated with ceftiofur and danofloxacin developed antibodies to A. pleuropneumoniae, and the microbe was reisolated from approximately 50% of these animals. In contrast, pigs treated with enrofloxacin did not develop antibodies to A. pleuropneumoniae, and the challenge strain was not found at necropsy. The performance with respect to daily weight gain and feed conversion corresponded well with the clinical signs developed and the findings made at necropsy. The decreased growth recorded during the acute phase of the disease was, to a large extent, caused by a reduced feed intake.</p>","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"46 4","pages":"249-60"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21246485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}