Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B最新文献_第10页

Investigations of the Japanese bovine tumour virus (BLV)--its ability to express structural and regulatory BLV proteins. 日本牛肿瘤病毒(BLV)的研究——其表达结构和调节BLV蛋白的能力。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00324.x

P Blankenstein, A Bondzio, A Büchel, E Kinder, D Ebner

引用次数: 6

Differentiation of Salmonella gallinarum and Salmonella pullorum by their whole-cell fatty acid methyl ester profiles. 鸡沙门氏菌和白痢沙门氏菌的全细胞脂肪酸甲酯谱鉴别。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00326.x

M Ryll, M Bisgaard, J P Christensen, K H Hinz

引用次数: 8

The efficacy of doramectin on arrested larvae of Ancylostoma caninum in early pregnancy of bitches. 多拉菌素对母狗妊娠早期犬钩虫捕获幼虫的影响。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00325.x

T Schnieder, M Lechler, C Epe, S Kuschfeldt, M Stoye

{"title":"The efficacy of doramectin on arrested larvae of Ancylostoma caninum in early pregnancy of bitches.","authors":"T Schnieder, M Lechler, C Epe, S Kuschfeldt, M Stoye","doi":"10.1111/j.1439-0450.1996.tb00325.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00325.x","url":null,"abstract":"The efficacy of doramectin treatment on arrested A. caninum larvae during early pregnancy of bitches was examined. Four bitches were percutaneously infected with 20,000 third-stage larvae of A. caninum on the day of conception and treated subcutaneously with 1 mg doramectin per kg body weight on day 30 of pregnancy. Four infected untreated pregnant bitches served as controls. A single application of dormectin substantially reduced the number of somatic larvae in bitches and the number of intestinal stages in bitches and puppies. However, it did not completely prevent lactogenic transmission of A. caninum larvae because five out of 23 puppies from three litters of the treated bitches harboured adult worms in their intestines, two of them shed eggs with the faeces. Although clinical disease did not occur in puppies from treated bitches the efficacy of the treatment was not satisfactory from an epidemiological point of view. Despite the treatment puppies with patent infections contaminated their environment with high numbers of eggs thus producing an intolerable infection risk for dogs and humans. No fetotoxic side-effects of the early treatment with doramectin were seen.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 6","pages":"351-6"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00325.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19765371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. 福尔马林固定和石蜡包埋禽组织衣原体的原位杂交检测。原位杂交与过氧化物酶-抗过氧化物酶标记的比较。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00327.x

D Theil, R Hoop, A J Herring, A Pospischil

{"title":"Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling.","authors":"D Theil, R Hoop, A J Herring, A Pospischil","doi":"10.1111/j.1439-0450.1996.tb00327.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00327.x","url":null,"abstract":"In situ hybridization, (ISH) using a digoxigenin-antisense RNA-probe to detect chlamydial rRNA was applied to post mortem tissue of birds. The technique was optimized and validated using tissue from experimentally-infected chicken embryos. Tissue sections were also tested by immunohistochemistry (peroxidase-antiperoxidase reaction, PAP) for the presence of chlamydial antigen using a genus specific monoclonal antibody. In the chicken embryo tissue, ISH and PAP were comparably sensitive and specific (100% and 100%, respectively). ISH and PAP in general were correlated to microscopic lesions. For further comparison, ISH with PAP was applied retrospectively to tissues of 82 birds from which Chlamydia had been isolated, or which were suggestive of chlamydiosis. Using in situ hybridization 47 of 82 birds were found to be positive, and as were 23 of 82 birds with PAP. None of the ISH-only positive cases were found to be strongly positive. On the other hand, cases which were found positive with the ISH were also positive with other methods (PAP and isolation of Chlamydiae from chicken embryos). There was no close correlation between the positive cells and histological lesions. In spite of the higher sensitivity and specificity of the ISH, this technique is not suitable for routine diagnostic investigations. ISH is expensive, laborious, and time consuming.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 6","pages":"365-70"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00327.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19765314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Experimental melioidosis in hens. 母鸡的实验性类鼻疽。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00328.x

A Vesselinova, H Najdenski, S Nikolova, V Kussovski

{"title":"Experimental melioidosis in hens.","authors":"A Vesselinova, H Najdenski, S Nikolova, V Kussovski","doi":"10.1111/j.1439-0450.1996.tb00328.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00328.x","url":null,"abstract":"Experimental intramuscular infection of hens with Pseudomonas pseudomallei, strain 2796 (1 x 10(9) CFU from a 24-h culture) was reproduced. Clinical, paraclinical and pathomorphological findings were followed from 1 to 30 days after challenge. Haemagglutinin titre, bacterial dissemination in the viscera, number of leucocytes, alveolar (aMa) and peritoneal (pMa) macrophages and their phagocytic activity in vitro were studied. During the course of infection a leucocytosis as well as an increased haemagglutinin titre (1:256) were established. The number of bacteria per gram tissue in the spleen and liver was highest at 1 day post-infection (p.i.). Melioidose bacteria from egg yolk were isolated at 15 and 30 days p.i. Leucocyte and pMa phagocytic activity was maximal at 3 days p.i. unlike the activity of aMa which increased gradually until the end of the study. Inflammatory-necrotic changes were found in the viscera and brain at 3 and 15 days p.i. The investigation of experimental melioidosis infection in hens showed that they are susceptible to P. pseudomallei and this disease takes a generalized subacute course.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 6","pages":"371-8"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00328.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19765315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive plaque assay and propagation of Chuzan (Kasba) virus, a Palyam serogroup orbivirus, in BHK-21 cells. 一种Palyam血清型眶病毒Chuzan (Kasba)病毒在BHK-21细胞中的敏感斑块测定和繁殖

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00323.x

N Hirano, T Tawara, R Nomura, A Imai, K Ono, R Yamaguchi

{"title":"Sensitive plaque assay and propagation of Chuzan (Kasba) virus, a Palyam serogroup orbivirus, in BHK-21 cells.","authors":"N Hirano, T Tawara, R Nomura, A Imai, K Ono, R Yamaguchi","doi":"10.1111/j.1439-0450.1996.tb00323.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00323.x","url":null,"abstract":"Various factors influencing plaque formation of Chuzan virus in BHK-21 cell monolayers were studied and a practical method for plaque assay was developed. On addition of trypsin (5 micrograms/ml) and/or diethylaminoethyl (DEAE)-dextran (50 micrograms/ml) to the virus diluent as the virus adsorption medium and agar overlay medium, the number of plaques increased. When 100 micrograms/ ml DEAE-dextran was added to the diluent and overlay medium, plaques were produced in about 10-fold higher numbers than without trypsin and DEAE-dextran. Based on these results, a practical plaque assay method for Chuzan virus was established. Using this method, one-step growth of Chuzan virus was performed at an input multiplicity of 25 plaque-forming units (PFU) per cell. Cytopathic effects were first observed at 7.5 h post-inoculation (p.i.), and were complete at 12 h p.i. The titre of cell-associated virus, after gradual decline during the first 3 h of incubation, showed a rise within 4.5 h p.i. and a rise to a plateau of 10(6.3)PFU/0.2 ml at 12 h p.i. By indirect immunofluorescence, virus-specific antigen was detected in the cytoplasm of the cells at 4.5 h p.i., and all the cells fluoresced at 6 h p.i. Haemagglutination activity was first detected in infected whole cultures at 7.5 h p.i. reaching a plateau of 1:64 at 15 h p.i. Plaque formation and haemagglutination by the virus were specifically inhibited by antisera against the original and the plaque-cloned virus.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 6","pages":"333-42"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00323.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19765368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bacterial causes of bovine mastitis in Wondogenet, Ethiopia. 埃塞俄比亚Wondogenet牛乳腺炎的细菌原因。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00329.x

M Abdella

引用次数: 13

Lymphoproliferation assays in cattle naturally infected with bovine leukaemia virus (BLV) and bovine immunodeficiency-like virus (BIV). 牛白血病病毒(BLV)和牛免疫缺陷样病毒(BIV)自然感染牛的淋巴细胞增殖测定。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-08-01 DOI: 10.1111/j.1439-0450.1996.tb00322.x

G Hidalgo, J A Bonilla

{"title":"Lymphoproliferation assays in cattle naturally infected with bovine leukaemia virus (BLV) and bovine immunodeficiency-like virus (BIV).","authors":"G Hidalgo, J A Bonilla","doi":"10.1111/j.1439-0450.1996.tb00322.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00322.x","url":null,"abstract":"Experiments were designed to evaluate the effect of BLV on mitogen-stimulated peripheral blood mononuclear cells (PBMC) from naturally infected cattle. BIV was also taken into consideration due to a recent report showing that in Costa Rica, most of the BLV-infected animals are also seropositive for BIV. The methodology was based on a non-radioactive technique to determine lymphoproliferation. A colorimetric assay using XTT (formazan salt) to measure cell multiplication was adapted for bovine PBMC. ELISA and Western blotting were used to determine the serologic status of the cattle. PCR was only available for BIV detection. Our results show clearly that, dually-infected cattle (BIV-BLV) have reduced lymphoproliferative responses to the mitogen Con A. Haematological abnormalities associated with viral infections were also observed, specially leukocytosis and lymphocytosis. Cows with lymphosarcomas are severely affected. The specific antibody response to different viral proteins could not be associated with the suppressive status of the animals. Due to the high rate of dual infections observed in Costa Rica, these results are not sufficient to clarify which virus is responsible for the suppressive activity, if one or both viruses are necessary, or if they act synergistically.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 6","pages":"325-32"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00322.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19765366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Commercially available antibodies to human interleukin-1 do not crossreact with porcine interleukin-1. 市售的人白细胞介素-1抗体不会与猪白细胞介素-1发生交叉反应。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-07-01 DOI: 10.1111/j.1439-0450.1996.tb00320.x

H Holst, U Magnusson

引用次数: 0

Immunohistochemical findings in eyes of cats serologically positive for feline immunodeficiency virus (FIV). 猫眼免疫组化血清学阳性猫免疫缺陷病毒(FIV)。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-07-01 DOI: 10.1111/j.1439-0450.1996.tb00318.x

G Loesenbeck, W Drommer, H F Egberink, H J Heider

{"title":"Immunohistochemical findings in eyes of cats serologically positive for feline immunodeficiency virus (FIV).","authors":"G Loesenbeck, W Drommer, H F Egberink, H J Heider","doi":"10.1111/j.1439-0450.1996.tb00318.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.1996.tb00318.x","url":null,"abstract":"In this study the eyes of 15 cats in the terminal stage of FIV infection were examined. The findings were compared to those in cats, which were euthanized because of other infectious diseases for for non-infectious reasons. Thirteen FIV-infected cats showed an anterior uveitis by means of light microscopy. No accumulation of retinal lesions were found in FIV-infected cats compared to the other cats examined. Additionally, there were no indications of lesions caused by opportunistic infections. In the posterior segments of the eyes, immunohistochemical examinations proved the plasma proteins C3 and IgG to be predominantly intravascular. The eyes of 11 serologically FIV-positive cats were available for immunohistochemical examination. In all 11 cats at least one of the plasma proteins C3 or IgG could be detected in the extravascular tissue of the anterior uvea. The extravascular presence of plasma proteins within the tissue seemed to be caused by an increased permeability of the vessels due to inflammation. Furthermore, the similar extravascular distribution pattern of IgG and complement component C3 in four cases indicated that immune complexes may play a role in the anterior uveitis of FIV-infected cats.","PeriodicalId":23829,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B","volume":"43 5","pages":"305-11"},"PeriodicalIF":0.0,"publicationDate":"1996-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.1996.tb00318.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19751644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8