单克隆抗体夹心酶联免疫吸附法检测鳗弧菌。

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-12-01 DOI:10.1111/j.1439-0450.1996.tb00356.x

R Adone, G Piccininno, C Pistoia, R Bianchi, F Ciuchini

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引用次数: 4

摘要

制备了抗鳗弧菌的单克隆抗体(mab)，并通过Western blotting分析、竞争结合试验和交叉反应性试验对其进行了鉴定。用夹心酶联免疫吸附试验(ELISA)测试了这些单克隆抗体作为捕获抗体或示踪抗体的不同组合，检测了它们在液体培养基中检测鳗弧菌的能力。选择一种组合作为最适合的诊断应用，显示出最高的灵敏度和特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Detection of Vibrio anguillarum by a sandwich enzyme-linked immunosorbent assay performed with monoclonal antibodies.

Monoclonal antibodies (Mabs) against V. anguillarum were produced and characterized by Western blotting analysis, competitive binding assays and cross-reactivity tests. Their ability to detect V. anguillarum in a liquid culture was tested in a sandwich enzyme-linked immunosorbent assay (ELISA) performed with different combinations of these Mabs used as capture or tracer antibodies. One combination was selected as the most suitable for diagnostic applications, showing the highest sensitivity and specificity.

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Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B

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