{"title":"[Experiences in the preparation of the secretory IgA and secretory component from human colostrum].","authors":"K H Vogt, A Groh, I Gruhn, M Hartmann, A Stelzner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>With the isolation of secretory IgA and secretory component from human colostrum following the route communicated by Kobayashi some differences revealed with respect to the preparative results. Mainly problems arising from lactoferrin and IgM encouraged us to offer some varied laboratory instructions.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"261 1","pages":"95-106"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14216861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Isolation and characterization of erythrogenic toxins. VIII. Purification of a biologically active protein of the molecular weight 10,000 (LMP-10k) from filtrates of Streptococcus pyogenes, strain NY-5. Relationship to erythrogenic toxin type A].","authors":"D Gerlach, J H Ozegowski, H Knöll, W Köhler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The \"classical\" method for purification of erythrogenic toxin type A results in two products: erythrogenic toxin type A and a low molecular weight protein, m.w. 10,000 (LMP-10k) with mitogenic activities. LMP-10k was purified from culture supernatants of S. pyogenes (group A) by CM-Sepharose CL-6B chromatography and Sephacryl S-200 and Sephadex G75 gel filtration to a high degree of purity with minimal amounts of residual erythrogenic toxin A. The isoelectric point of LMP-10k is the same as for erythrogenic toxin A: 5.2. The immunogenic activity is low, only one of two rabbits produced anti-LMP-10k-antibodies after a prolonged course of immunization. On the other hand it is possible to induce antierythrogenic toxin A-antibodies by immunization with LMP-10k preparations contaminated with small amounts of erythrogenic toxin A. Possibly the data given by some authors for the m.w. of erythrogenic toxin type A as 8 000 D are the results of a mix-up with co-purified LMP-10k.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"261 1","pages":"75-84"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14641689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Experimental ocular cryptococcosis under the influence of time-limited kidney failure--a pathohistological study].","authors":"G Grosse","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This animal experiment is characterized by a missing virulence of Cryptococcus neoformans strain A94 (isolated from bird manure) for the immunocompetent mouse after intraperitoneal injection and following hematogenous dissemination into all organs including the central nervous system (CNS) and by the induction of a renal failure of short duration by intramuscular injection of glycerine. In 50% of the group of 80 animals damaged in such a way ocular cryptococcosis was found in association with the selective involvement of the CNS. The involvement of the eyes was dependent on a time-limited interval between the date of infection and the date of induction of the renal failure. The partly extensive pathohistological findings in the eye, with a predominance of mycotic processes in the retina and with some cases of endophthalmitis, were found to be similar to those described in man. The animal experiment described is proposed as a model for experimental studies to resolve problems of the immunology and therapy of the ocular cryptococcosis which are of current interest.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 4","pages":"550-65"},"PeriodicalIF":0.0,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15031529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Morphology of new Listeria phages].","authors":"S Ortel, H W Ackermann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Seventeen Listeria phages have isometric heads and long, noncontractile tails. Twelve of them belong to three already known phage species. Five phages represent a new species, named H387.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 4","pages":"423-7"},"PeriodicalIF":0.0,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14951420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Selection of R plasmid-carrying E. coli pathogens in a mixed population by subinhibitory antibiotic concentrations].","authors":"R Egger, G Lebek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of subinhibitory concentrations of tetracyclin, chloramphenicol, gentamicin and ampicillin in a mixed culture of E. coli was investigated in a chemostat in order to determine minimal selection concentration of these drugs. The mixed culture consisted of three clones of E. coli K12 921. One of these clones was plasmid free, one harboured the F'lac plasmid and the third one an R-factor. The antibiotics tetracyclin, chloramphenicol and gentamicin selected the antibiotic resistant R-factor bearing strain at a concentration of 1/10 of the minimal inhibition concentration. Subinhibitory concentrations of ampicillin failed to select the R-factor bearing strain because this strain produced a secreted betalactamase.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 3","pages":"361-8"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15026073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Salmonella eschberg--report on the isolation of a new serovar].","authors":"H P Rohr","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The serological and biochemical characteristics of a so far unknown serovar of Salmonella subspecies I with the serological formula 9,12:d:1,7 is described. It has been named after a borough of Saarbrücken: Salmonella eschberg.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 3","pages":"339-41"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15202207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Determination of the number of pathogens in bacterial suspensions using laser nephelometry in comparison to the photometry and plate counting method].","authors":"R Sonak, H Thoholte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We determined the number of bacteria in suspensions of three bacterial species (S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853) in liquid medium using two optical methods - laser-nephelometry and photometry. These results were compared with the CFU/ml determined by the agar plate test in each suspension. Further we defined the applicable range of measurement of both optical methods. The calculated factor of correlation (r = 0.99) attested the very good conformity of the results obtained by both optical methods. The determination of the number of bacteria in suspensions by laser-nephelometry is a simple though very sensitive method. The results can well be reproduced. A further advantage of this method is the immediate information about the number of bacteria in suspension. All these facts recommend laser-nephelometry as a method useful in any bacteriological routine laboratory for determination of the number of bacteria in suspensions.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 3","pages":"329-38"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15053578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Biochemical characterization of species in the genus Listeria].","authors":"J Rocourt, B Catimel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Seventy Listeria strains, including L. monocytogenes (30 strains), L. ivanovii (9), L. innocua (11), L. welshimeri (6), L. seeligeri (10), L. grayi (2) and L. murrayi (2) were subjected to biochemical characterization. The biochemical tests selected comprised: Api 50CH, Api 20E and Api Zym strips, hydrolysis of DNA and tween 20, 40, 60, 80, egg yolk agar reaction, growth in the presence of 7,5 and 10% NaCl, growth at 42 degrees C and 45 degrees C, and susceptibility to antibiotics and trypaflavin. No further marker in addition to previously described ones, allowing to distinguish L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri and L. seeligeri could be detected. L. grayi and L. murrayi differed from the other Listeria species by gluconate fermentation, lack of phosphoamidase and acid phosphatase and susceptibility to trypaflavin.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 2","pages":"221-31"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15050225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Bacteriology and chemotherapy].","authors":"G Linzenmeier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In a farewell lecture the relevance of bacteriology for the education of medical students is described. In the times of Koch and Pasteur the bacteriological diagnostic of epidemic diseases was the main problems, to day it is the isolation and differentiation of the patient's own bacteria which give the background for resistance or sensitivity tests to chemotherapeutics. The choice of antimicrobial substances depends not only on the sensitivity of the individual bacterial strain or the toxicology of certain substances but also on the necessity to preserve the normal microecology of the gut and other biotopes. This normal bacterial colonisation is an important factor of colonial resistance against superinfection. Other points for the choice of antimicrobial substances are the epidemiological distribution of resistant strains and the possible interference between the immunological system and certain antibiotics.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 2","pages":"165-74"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15193883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Typing of Streptococcus faecalis and Streptococcus faecium strains with bacteriophages].","authors":"H Brandis, P Plećeas, L Andries","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>With a set of 17 bacteriophages specific for Streptococcus faecalis and a set of 7 bacteriophages specific for Streptococcus faecium 441 strains of S. faecalis and 53 strains of S. faecium were typed. The species identification was done by means of the API 20 STREP system. For S. faecalis 22 and for S. faecium 12 lysotypes were found. Among S. faecalis strains lysotype V6b was most common (27%), followed by the lysotypes XV (9,5%), XIII1 (7%), II1 (5,7%), and I1 (4,3%). These 5 lysotypes comprised 54% of all 441 strains. 21% of the strains of S. faecalis and 6 from 53 strains of S. faecium were untypable. Strains of S. faecium were most frequently isolated from faeces and in rare cases from urine. To the contrary 50% of the S. faecalis strains were isolated from urine. There was no correlation between special lysotypes and age of the patients or the source of specimen. The results of phage typing of several strains from one patient, and especially of strains from mothers and their newborns suggest the usefulness of the typing system to clarify epidemiological questions.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"260 2","pages":"206-15"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15051876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}