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Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology最新文献

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[A new ELISA micromethod for the determination of tetanus antibodies]. [一种新的ELISA微量法测定破伤风抗体]。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-12-01
F Ambrosch, G Wiedermann, H Müller
{"title":"[A new ELISA micromethod for the determination of tetanus antibodies].","authors":"F Ambrosch,&nbsp;G Wiedermann,&nbsp;H Müller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Due to its simplicity, accuracy and economy, the ELISA technique is a suitable method also for the determination of tetanus antibodies. By the method described, a titration curve is established from each serum to be investigated as well as from a standard serum. The horizontal distance between standard and unknown serum expressed in log2 titre steps is called titre difference. From this titre difference and the concentration of the standard serum the concentration of the unknown serum can be calculated. By means of an appropriate computer this evaluation can be done automatically. The variation coefficient with regard to concentration was 18.3%. A comparison with the toxin neutralisation test showed a correlation coefficient of 0.87. The application of this method to various problems is described.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"258 2-3","pages":"173-82"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17458317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Degradation of glutamic acid and proline in Clostridium sordellii under cadaveric bacteriological inspection]. [尸体细菌学检查下梭状芽孢杆菌对谷氨酸和脯氨酸的降解]。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-10-01
W Huckenbeck, T Daldrup
{"title":"[Degradation of glutamic acid and proline in Clostridium sordellii under cadaveric bacteriological inspection].","authors":"W Huckenbeck,&nbsp;T Daldrup","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Glutamic acid (GLU) is decarboxylated to gamma-aminobutyric acid (GABA) by Clostridium sordellii isolated from a decaying human brain. The dependence of this reaction on temperature, pH and substrate concentration has been established. The pH-optimum is in the range of 5.0 to 5.2. Near optimal pH the temperature optimum is greater than 28 degrees C. At higher pH-values (6 to 7) activity is relatively independent of temperature. The similarity to results in decaying human brains (10, 11, 12) shows a good correlation between postmortem bacterial flora and GLU-degradation. Furthermore it is shown that C. sordellii produces delta-aminovaleric acid (AVA) from proline (PRO).</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"258 1","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17218081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effect of iron on Pasteurella multocida]. [铁对多杀巴氏杆菌的影响]。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-10-01
K D Flossmann, G Müller, P Heilmann, H Rosner
{"title":"[Effect of iron on Pasteurella multocida].","authors":"K D Flossmann,&nbsp;G Müller,&nbsp;P Heilmann,&nbsp;H Rosner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Iron is an important factor for growth, virulence and immunogenicity of the species Pasteurella multocida. This has been demonstrated in numerous experiments with bacterial cultures in vitro and immunized and not immunized animals in vivo (mice, piglets, calves). Iron substrates or iron chelators affect in different manner the virulence of P. multocida in vivo, depending on chemical character of the given compounds, their dose, route and time of application, and also depending on the host. P. multocida has an up to time unknown iron transport system, which can acquire the essential iron from physiological substances, such as heme, ferritine, transferrine, lactoferrine etc. This conclusion results from in vitro experiments with growing cultures, with insertion of radioactive iron (Fe-59) from different sources, and with iron solubilization in neutral pH ranges. In the same way, the iron of iron dextran and low molecular iron compounds is available for P. multocida. Iron of unphysiological complexes, potassium ferrocyanide, and ferrocene is unavailable. On the other side such iron chelating agents as nitrilotriacetate, tirone, ferrocene, citrate, EDTA, and apotransferrine do not or only a little affect growth, and such chelators as alpha, alpha'-dipyridyle, phenanthroline and the microbial siderophores deferrioxamin B and enterobactin are inhibitory substances for multiplication of P. multocida. This substances also inhibit the insertion of Fe-59 into the bacterial cell. The conclusion is drawn that neither enterobactin nor deferrioxamine B as typical representatives of phenolate or hydroxamate siderophores take part in Fe-transport of P. multocida.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"258 1","pages":"80-93"},"PeriodicalIF":0.0,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17582992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Formation of alpha-aminobutyric acid in Clostridium sordellii]. [梭状芽孢杆菌中α -氨基丁酸的形成]。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-10-01
W Huckenbeck, T Daldrup
{"title":"[Formation of alpha-aminobutyric acid in Clostridium sordellii].","authors":"W Huckenbeck,&nbsp;T Daldrup","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After a modified growth C. sordellii is able to ferment threonine as mono-substrate. It could be demonstrated for the first time that alpha-aminobutyric acid is definitely formed from threonine by clostridia. The pH-optimum of this reaction is greater than 8, the temperature optimum is greater than 28 degrees C. Further fermenting products are: glycine and presumably acetaldehyde.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"258 1","pages":"62-8"},"PeriodicalIF":0.0,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17499341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Quality assurance in medical microbiology]. [医学微生物学的质量保证]。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-10-01
F Burkhardt
{"title":"[Quality assurance in medical microbiology].","authors":"F Burkhardt","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"258 1","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17455146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunohistochemical investigations of leptospira-infected golden hamsters in potency tests. 钩端螺旋体感染金仓鼠效力试验免疫组化研究。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-09-01
B Hein, H Freudenstein
{"title":"Immunohistochemical investigations of leptospira-infected golden hamsters in potency tests.","authors":"B Hein,&nbsp;H Freudenstein","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Golden hamsters taken from routine potency testing of leptospirosis vaccines were investigated for leptospirae after a challenge infection. Kidneys, livers, and spleens were examined immunohistochemically by the unlabelled antibody enzyme (PAP) method, and samples of these organs were additionally cultured for the evidence of living leptospirae. In non-vaccinated animals the recultivation method indicated leptospirae during the bacteraemic phase, beginning immediately after infection. Both methods resulted in positive reactions after organ manifestations. Intravascular evidence of leptospirae in the kidneys could be seen first on day 3 after infection. On day 7, there was intracellular and intratubular evidence as well, combined with tubulo-interstitial alterations. In vaccinated animals the bacteraemia after infection was reduced. Organ manifestations were seen rarely; there were less severe or no pathohistological alterations.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"257 4","pages":"521-5"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17451434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of an immuno-enzyme technique to titration of antibodies in leptospirosis: ELISA (enzyme-linked immunosorbent assay). 应用免疫酶技术测定钩端螺旋体病抗体:ELISA(酶联免疫吸附试验)。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-09-01
M Mailloux, J G Mazzonelli, Y Dufresne
{"title":"Application of an immuno-enzyme technique to titration of antibodies in leptospirosis: ELISA (enzyme-linked immunosorbent assay).","authors":"M Mailloux,&nbsp;J G Mazzonelli,&nbsp;Y Dufresne","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"257 4","pages":"511-3"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17562783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Survival of leptospires in commercial blood culture systems. 钩体在商业血液培养系统中的存活。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-09-01
M Palmer, S A Waitkins, W Zochowski
{"title":"Survival of leptospires in commercial blood culture systems.","authors":"M Palmer,&nbsp;S A Waitkins,&nbsp;W Zochowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Laboratory diagnosis of leptospirosis depends on the serological findings and, if possible, the isolation of the organisms. During the period 1981-1982 there were 40 blood cultures submitted from serologically positive patients and a successful isolation rate of only 12% was attained. Correct media, method of collection of suspect blood and laboratory technique all contribute to the successful isolation of the organism. Because of the low isolation rate an investigation was undertaken of various blood culturing systems used in Great Britain. Results obtained showed that certain blood culture media were able to maintain the organism better and therefore increase the isolation rates of leptospira. Factors affecting the isolation rate are presented.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"257 4","pages":"480-7"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17162411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differentiation of pathogenic and saprophytic leptospira strains. 致病性和腐生性钩端螺旋体的鉴别。
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-09-01 DOI: 10.1016/S0176-6724(84)80077-2
S. Bazovská, E. Kmety, J. Rak
{"title":"Differentiation of pathogenic and saprophytic leptospira strains.","authors":"S. Bazovská, E. Kmety, J. Rak","doi":"10.1016/S0176-6724(84)80077-2","DOIUrl":"https://doi.org/10.1016/S0176-6724(84)80077-2","url":null,"abstract":"","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"1 1","pages":"517-20"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76580425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
May human leptospirosis develop as a chronic infection? 人类钩端螺旋体病可以发展为慢性感染吗?
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Pub Date : 1984-09-01
M Nicolescu, N Andreescu
{"title":"May human leptospirosis develop as a chronic infection?","authors":"M Nicolescu,&nbsp;N Andreescu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In connection with some rather uncommon cases, the authors asked themselves whether leptospirosis may also develop as a chronic infection. To answer this question, we took into consideration four of the most representative investigated cases encountered during the last 22 years. In conclusion, it appears that in some circumstances, human leptospirosis could present itself as a chronic disease.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"257 4","pages":"531-4"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17562784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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