[Biochemical characterization of species in the genus Listeria].

J Rocourt, B Catimel
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引用次数: 0

Abstract

Seventy Listeria strains, including L. monocytogenes (30 strains), L. ivanovii (9), L. innocua (11), L. welshimeri (6), L. seeligeri (10), L. grayi (2) and L. murrayi (2) were subjected to biochemical characterization. The biochemical tests selected comprised: Api 50CH, Api 20E and Api Zym strips, hydrolysis of DNA and tween 20, 40, 60, 80, egg yolk agar reaction, growth in the presence of 7,5 and 10% NaCl, growth at 42 degrees C and 45 degrees C, and susceptibility to antibiotics and trypaflavin. No further marker in addition to previously described ones, allowing to distinguish L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri and L. seeligeri could be detected. L. grayi and L. murrayi differed from the other Listeria species by gluconate fermentation, lack of phosphoamidase and acid phosphatase and susceptibility to trypaflavin.

[李斯特菌属物种的生化特征]。
对70株李斯特菌进行了生化鉴定,包括单增李斯特菌(30株)、李斯特菌(9株)、李斯特菌(11株)、李斯特菌(6株)、李斯特菌(10株)、李斯特菌(2株)和李斯特菌(2株)。选择的生化试验包括:Api 50CH、Api 20E和Api Zym试条,DNA和吐温20、40、60、80的水解,蛋黄琼脂反应,7、5和10% NaCl条件下的生长,42℃和45℃条件下的生长,抗生素和锥黄素的敏感性。除了先前描述的标记外,没有进一步的标记可用于区分单核增生乳杆菌、伊万诺维奇乳杆菌、伊万诺维奇乳杆菌、韦氏乳杆菌和塞利格里乳杆菌。与其他李斯特菌不同的是,灰色李斯特菌和穆拉李斯特菌具有葡萄糖酸发酵、缺乏磷酸酰胺酶和酸性磷酸酶以及对胰黄素敏感的特点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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