World Journal of Microbiology and Biotechnology最新文献_第8页

Microbiological properties of Beejamrit, an ancient Indian traditional knowledge, uncover a dynamic plant beneficial microbial network. Beejamrit的微生物特性，一个古老的印度传统知识，揭示了一个动态的植物有益微生物网络。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-16 DOI: 10.1007/s11274-022-03296-3

Shibasis Mukherjee, Suchana Sain, Md Nasim Ali, Rupak Goswami, Argha Chakraborty, Krishnendu Ray, Rantim Bhattacharjee, Bhubaneswar Pradhan, Natesan Ravisankar, Gautam Chatterjee

{"title":"Microbiological properties of Beejamrit, an ancient Indian traditional knowledge, uncover a dynamic plant beneficial microbial network.","authors":"Shibasis Mukherjee, Suchana Sain, Md Nasim Ali, Rupak Goswami, Argha Chakraborty, Krishnendu Ray, Rantim Bhattacharjee, Bhubaneswar Pradhan, Natesan Ravisankar, Gautam Chatterjee","doi":"10.1007/s11274-022-03296-3","DOIUrl":"10.1007/s11274-022-03296-3","url":null,"abstract":"Beejamrit is an ancient organic formulation commonly used as a seed treatment in organic and natural farming in India. This low-cost formulation is primarily a product of dairy excreta (e.g., cow dung and cow urine) and forest soil, often supplemented with limestone. Growing data suggest that dairy excreta are the potential sources of enriched microbial niche, including several plant growth-promoting bacteria capable of synthesizing plant growth regulators. However, the microbiological properties of Beejamrit and their temporal changes after different incubation periods, delineating its application in seed treatment, remain largely unexplored. Here, we aimed to analyze the decomposition rate of Beejamrit over 7-consecutive days of incubation. This study further elucidates the microbial niche and their dynamics in Beejamrit, including the plant beneficial bacteria. We have shown that the population of plant beneficial bacteria, such as the free-living nitrogen fixers (FNFs) and the phosphate solubilizers (PSBs), proliferates progressively up to 4- and 5-days of incubation, respectively (p < 0.0001). This study also reports the total indolic content of Beejamrit, including indole 3-acetic acid (IAA), which further tends to oscillate in concentration based on the incubation periods incurred during the Beejamrit preparation. Our analyses, together, establish that Beejamrit provides a dynamic, microbe-based metabolic network and may, therefore, act as a plant biostimulant to crop plants. A plant-based bioassay finally demonstrates the role of Beejamrit in the seed treatment to improve seed germination, seedling survival rate, and shoot length trait in French beans (p < 0.01). In conclusion, this study highlights, for the first time, the scientific insights of Beejamrit as a potential seed priming agent in agriculture.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"56 1","pages":"111"},"PeriodicalIF":0.0,"publicationDate":"2022-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91334732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the biological treatability of pistachio processing industry wastewaters in a batch-operated aerobic bioreactor. 间歇式好氧生物反应器处理开心果工业废水的研究。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-12 DOI: 10.1007/s11274-022-03292-7

Sevtap Tırınk, Alper Nuhoğlu, Sinan Kul

{"title":"Investigation of the biological treatability of pistachio processing industry wastewaters in a batch-operated aerobic bioreactor.","authors":"Sevtap Tırınk, Alper Nuhoğlu, Sinan Kul","doi":"10.1007/s11274-022-03292-7","DOIUrl":"10.1007/s11274-022-03292-7","url":null,"abstract":"This study encompasses investigation of treatment of pistachio processing industry wastewaters in a batch reactor under aerobic conditions, calculation of kinetic parameters and comparison of different inhibition models. The mixed microorganism culture used in the study was adapted to pistachio processing industry wastewaters for nearly one month and then concentrations from 50 to 1000 mg L-1 of pistachio processing industry wastewaters were added to the medium and treatment was investigated in batch experiments. The Andrews, Han-Levenspiel, Luong and Aiba biokinetic equations were chosen for the correlations between the concentration of pistachio processing industry wastewaters and specific growth rates, and the kinetic parameters in these biokinetic equations were calculated. The maximum specific growh rate, semi-saturated constant and inhibition constant parameters, included in the Aiba biokinetic equation providing best fit among the other equations, had values calculated as 0.25 h-1, 19 mg L-1, and 516 mg L-1, respectively. The substrate value reaches maximum value and the specific growth rate at this concentration were calculated as 101.379 mg L-1 and 0.1827 h-1, respectively.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"6 1","pages":"109"},"PeriodicalIF":0.0,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79424817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-microbe hybrid synthesis provides new insights for the efficient use of Macleaya cordata. 植物-微生物杂交合成技术为高效利用麦草籽提供了新的思路。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-12 DOI: 10.1007/s11274-022-03295-4

Mengshan Sun, Xiaohong Zhong, Li Zhou, Zixuan Xu, Peng Huang, Jianguo Zeng

{"title":"Plant-microbe hybrid synthesis provides new insights for the efficient use of Macleaya cordata.","authors":"Mengshan Sun, Xiaohong Zhong, Li Zhou, Zixuan Xu, Peng Huang, Jianguo Zeng","doi":"10.1007/s11274-022-03295-4","DOIUrl":"10.1007/s11274-022-03295-4","url":null,"abstract":"Sanguinarine and chelerytrine have antibacterial and anti-inflammatory effects and is the main active ingredients of growth promoters in animals. Currently, Sanguinarine and chelerytrine were extracted from the capsules of the medicinal plant Macleaya cordata. However, the biomass of M. cordata nonmedicinal parts (leaves) accounted for a large proportion and contained a rich presentation of protopine and allocryptopine which are the precursor compounds of sanguinarine and chelerytrine. The aim of this study was to develop a new method for producing sanguinarine and chelerytrine through yeast transformation of protopine and allocryptopine in M. cordata leaves. First, we isolated different genes from Papaver somniferum (PsP6H, PsCPR, PsDBOX), Eschscholtzia californica (EcP6H), Cucumis sativus (CuCPR), Arabidopsis thaliana (AtCPR) and M. cordata (Mc11229, Mc11218, Mc6408, Mc6407, Mc19967, Mc13802). Additionally, some of the gene sequences were codon optimized. Then, we transformed these genes into yeast cells to compare the catalytic efficiency. Second, we used the most efficient strains to biotransform the leaves of M. cordata. Finally, we obtained 85.415 ± 11.887 ng mL-1 sanguinarine and 4.288 ± 1.395 ng mL-1 chelerytrine, which was more than 2-3 times the content in leaves of M. cordata. Overall, we using the nonmedicinal parts of M. cordata and successfully obtained sanguinarine and chelerytrine by the plant-microbial hybrid synthesis method.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"92 4 1","pages":"110"},"PeriodicalIF":0.0,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83672117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of technical hexachlorocyclohexane by Cupriavidus malaysiensis. 大马铜霉降解工业用六氯环己烷的研究。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-09 DOI: 10.1007/s11274-022-03284-7

Vartika Srivastava, Shekhar Dhuliya, M Suresh Kumar

{"title":"Biodegradation of technical hexachlorocyclohexane by Cupriavidus malaysiensis.","authors":"Vartika Srivastava, Shekhar Dhuliya, M Suresh Kumar","doi":"10.1007/s11274-022-03284-7","DOIUrl":"10.1007/s11274-022-03284-7","url":null,"abstract":"Lindane (γ-Hexachlorocyclohexane) has been used extensively as a pesticide all over the world. The production of Lindane entails the formation of four major Hexachlorocyclohexane (HCH) isomers, that is, alpha, beta, gamma, and delta as muck. These have been used as Technical HCH in developing countries as an inexpensive alternate source. However, HCH isomers pose a severe environmental hazard due to their highly persistent nature and toxicity. In this study, the effect of HCH application on the soil microbial diversity was studied. The species which could persist even after prolonged exposure at high HCH concentration, was isolated, screened, and enriched as potential t-HCH degraders. The selected isolate could degrade 88.05%, 92.19%, 91.54%, and 82.85% of the alpha, gamma, beta, and delta isomers, respectively at 100 mg/L HCH concentration. Identification of the isolate by 16s rRNA sequencing was similar to Cupriavidus malaysiensis. To the best of the authors' knowledge, this is the first study to observe this particular strain's ability to simultaneously degrade the four isomers, especially the most recalcitrant beta isomer. Therefore, the degradative capability of this strain, as a sole carbon source at higher HCH concentration (100 mg/l), can be exploited for bioremediation of HCH contaminated sites.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"1 1","pages":"108"},"PeriodicalIF":0.0,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73465177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liamocin overproduction by the mutants of Aureobasidium melanogenum 9-1 for effectively killing spores of the pathogenic fungi from diseased human skin by Massoia lactone. 黑毛小孢子菌9-1突变体过量产生Liamocin以有效杀死马尾内酯致病菌孢子。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-04 DOI: 10.1007/s11274-022-03290-9

Zhu Wang, Mei Zhang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi

引用次数: 0

Directed evolution of the PobR allosteric transcription factor to generate a biosensor for 4-hydroxymandelic acid. 定向进化生成4-羟基扁桃酸生物传感器的PobR变构转录因子。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-03 DOI: 10.1007/s11274-022-03286-5

YaoYao Liang, Juan Luo, Chenhao Yang, Shuning Guo, Bowen Zhang, Fengqianrui Chen, Kairui Su, Yulong Zhang, Yi Dong, Zhihao Wang, Hongda Fu, Guangchao Sui, Pengchao Wang

引用次数: 0

Actinobacteria isolated from wastewater treatment plants located in the east-north of Algeria able to degrade pesticides. 从位于阿尔及利亚东北方的污水处理厂分离出的放线菌能够降解农药。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-03 DOI: 10.1007/s11274-022-03282-9

Oumeima Boufercha, Irina S Moreira, Paula M L Castro, Allaoueddine Boudemagh

{"title":"Actinobacteria isolated from wastewater treatment plants located in the east-north of Algeria able to degrade pesticides.","authors":"Oumeima Boufercha, Irina S Moreira, Paula M L Castro, Allaoueddine Boudemagh","doi":"10.1007/s11274-022-03282-9","DOIUrl":"10.1007/s11274-022-03282-9","url":null,"abstract":"The pollution of water resources by pesticides poses serious problems for public health and the environment. In this study, Actinobacteria strains were isolated from three wastewater treatment plants (WWTPs) and were screened for their ability to degrade 17 pesticide compounds. Preliminary screening of 13 of the isolates of Actinobacteria allowed the selection of 12 strains with potential for the degradation of nine different pesticides as sole carbon source, including aliette, for which there are no previous reports of biodegradation. Evaluation of the bacterial growth and degradation kinetics of the pesticides 2,4-dichlorophenol (2,4-DCP) and thiamethoxam (tiam) by selected Actinobacteria strains was performed in liquid media. Strains Streptomyces sp. ML and Streptomyces sp. OV were able to degrade 45% of 2,4-DCP (50 mg/l) as the sole carbon source in 30 days and 84% of thiamethoxam (35 mg/l) in the presence of 10 mM of glucose in 18 days. The biodegradation of thiamethoxam by Actinobacteria strains was reported for the first time in this study. These strains are promising for use in bioremediation of ecosystems polluted by this type of pesticides.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"1 1","pages":"105"},"PeriodicalIF":0.0,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91111391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial efficacy of in vitro and ex vivo photodynamic therapy using porphyrins against Moraxella spp. isolated from bovine keratoconjunctivitis. 体外和体外光动力治疗卟啉对牛角膜结膜炎分离的莫拉菌的抑菌效果。

World Journal of Microbiology and Biotechnology Pub Date : 2022-05-03 DOI: 10.1007/s11274-022-03291-8

M G Seeger, C S Machado, B A Iglesias, F S F Vogel, J F Cargnelutti

{"title":"Antimicrobial efficacy of in vitro and ex vivo photodynamic therapy using porphyrins against Moraxella spp. isolated from bovine keratoconjunctivitis.","authors":"M G Seeger, C S Machado, B A Iglesias, F S F Vogel, J F Cargnelutti","doi":"10.1007/s11274-022-03291-8","DOIUrl":"10.1007/s11274-022-03291-8","url":null,"abstract":"Infectious bovine keratoconjunctivitis (IBK) is an ocular disease affecting bovine herds worldwide, and it causes significant economic loss. The etiologic agent of IBK is considered to be Moraxella bovis, but M. ovis and M. bovoculi are frequently recovered of animals presenting clinical signs of IBK. The therapeutic measures available for its control have limited efficacy. Antimicrobial photodynamic therapy (aPDT) using porphyrins as photosensitizing molecules is an alternative method that can be used to reduce microbial growth. We evaluated the antibacterial activity of aPDT using two water-soluble tetra-cationic porphyrins (H2TMeP and ZnTMeP) against 22 clinical isolates and standard strains of Moraxella spp. in vitro and in an ex vivo model. For the in vitro assay, 4.0 µM of porphyrin was incubated with approximately 1.0 × 104 CFU/mL of each Moraxella sp. isolate and exposed to artificial light for 0, 2.5, 5, and 7.5 min. Next, 50 µL of this solution was plated and incubated for 24 h until CFU measurement. For the ex vivo assay, corneas excised from the eyeballs of slaughtered cattle were irrigated with Moraxella spp. culture, followed by the addition of zinc(II) porphyrin ZnTMeP (4.0 μM). The corneal samples were irradiated for 0, 7.5, and 30 min, followed by swab collection, plating, and CFU count. The results demonstrated the in vitro inactivation of the strains and clinical isolates of Moraxella spp. after 2.5 min of irradiation using ZnTMeP, reaching complete inactivation until 7.5 min. In the ex vivo experiment, the use of ZnTMeP resulted in the most significant reduction in bacterial concentration after 30 min of irradiation. These results encourage future in vivo experiments to investigate the role of metalloporphyrin ZnTMeP in the inactivation of Moraxella spp. isolates causing IBK.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"92 1","pages":"103"},"PeriodicalIF":0.0,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80405737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanobacterial secondary metabolites towards improved commercial significance through multiomics approaches. 通过多组学方法提高蓝藻次级代谢物的商业意义。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-29 DOI: 10.1007/s11274-022-03285-6

Shaloo Verma, Shobit Thapa, Nahid Siddiqui, Hillol Chakdar

{"title":"Cyanobacterial secondary metabolites towards improved commercial significance through multiomics approaches.","authors":"Shaloo Verma, Shobit Thapa, Nahid Siddiqui, Hillol Chakdar","doi":"10.1007/s11274-022-03285-6","DOIUrl":"10.1007/s11274-022-03285-6","url":null,"abstract":"Cyanobacteria are ubiquitous photosynthetic prokaryotes responsible for the oxygenation of the earth's reducing atmosphere. Apart from oxygen they are producers of a myriad of bioactive metabolites with diverse complex chemical structures and robust biological activities. These secondary metabolites are known to have a variety of medicinal and therapeutic applications ranging from anti-microbial, anti-viral, anti-inflammatory, anti-cancer, and immunomodulating properties. The present review discusses various aspects of secondary metabolites viz. biosynthesis, types and applications, which highlights the repertoire of bioactive constituents they harbor. Majority of these products have been produced from only a handful of genera. Moreover, with the onset of various OMICS approaches, cyanobacteria have become an attractive chassis for improved secondary metabolites production. Also the intervention of synthetic biology tools such as gene editing technologies and a variety of metabolomics and fluxomics approaches, used for engineering cyanobacteria, have significantly enhanced the production of secondary metabolites.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"1 1","pages":"100"},"PeriodicalIF":0.0,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89750329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for assessing the quality of AM fungal bio-fertilizer: Retrospect and future directions. AM真菌生物肥料质量评价方法回顾与展望。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-28 DOI: 10.1007/s11274-022-03288-3

R Agnihotri, M P Sharma, H Bucking, J F Dames, D J Bagyaraj

{"title":"Methods for assessing the quality of AM fungal bio-fertilizer: Retrospect and future directions.","authors":"R Agnihotri, M P Sharma, H Bucking, J F Dames, D J Bagyaraj","doi":"10.1007/s11274-022-03288-3","DOIUrl":"10.1007/s11274-022-03288-3","url":null,"abstract":"In the recent past, the mass production of arbuscular mycorrhizal (AM) fungi has bloomed into a large biofertilizer industry. Due to their obligate symbiotic nature, these fungi are propagated on living roots in substrate-based pot cultures and RiTDNA in in vitro or root organ culture systems. The quality assessment of AM inocula remains critical for the production and efficacy evaluation of AM fungi. The vigour of AM inocula are assessed through microscopic methods such as inoculum potential, infectivity potential/infection units, most probable number (MPN) and spore density. These methods marginally depend on the researcher's skill. The signature lipids specific to AM fungi, e.g. 16:1ω5cis ester-linked, phospholipid, and neutral lipid fatty acids provide more robustness and reproducibility. The quantitative real-time PCR of AM fungal taxa specific primers and probes analyzing gene copy number is also increasingly used. This article intends to sensitize AM fungal researchers and inoculum manufacturers to various methods of assessing the quality of AM inocula addressing their merits and demerits. This will help AM producers to fulfil the regulatory requirements ensuring the supply of high-quality AM inocula to end-users, and tap a new dimension of AM research in the commercial production of AM fungi and its application in sustainable plant production systems.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"29 1","pages":"97"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77924542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0