World Journal of Microbiology and Biotechnology最新文献

{"title":"An efficient approach for overproduction of DNA polymerase from Pyrococcus furiosus using an optimized autoinduction system in Escherichia coli","authors":"Moch Irfan Hadi, Fina Amreta Laksmi, Helbert, Arfena Rizqi Amalia, Azriel Dafa Muhammad, Wiga Alif Violando","doi":"10.1007/s11274-024-04127-3","DOIUrl":"https://doi.org/10.1007/s11274-024-04127-3","url":null,"abstract":"<p>High fidelity DNA polymerase from <i>Pyrococcus furiosus</i> (Pfupol) is an attractive alternative to the highly popular DNA polymerase from <i>Thermus aquaticus</i>. Because this enzyme is in great demand for biotechnological applications, optimizing Pfupol production is essential to supplying the industry’s expanding demand. T7-induced promoter expression in <i>Escherichia coli</i> expression systems is used to express recombinant Pfupol; however, this method is not cost-effective. Here, we have effectively developed an optimized process for the autoinduction approach of Pfupol expression in a defined medium. To better examine Pfupol’s activities, its purified fraction was used. A 71 mg/L of pure Pfupol was effectively produced, resulting in a 2.6-fold increase in protein yield when glucose, glycerol, and lactose were added in a defined medium at concentrations of 0.05%, 1%, and 0.6%, respectively, and the condition for production in a 5 L bioreactor was as follow: 200 rpm, 3 vvm, and 10% inoculant. Furthermore, the protein exhibited 1445 U/mg of specific activity when synthesized in its active state. This work presents a high level of Pfupol production, which makes it an economically viable and practically useful approach.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different trophic conditions on total fatty acids, amino acids, pigment and gene expression profiles in Euglena gracilis","authors":"Wen Hui Zhang, Jin Wei Gao, Cher Chien Lau, Zhi Fei Jiang, Yik Sung Yeong, Wen Jye Mok, Wenli Zhou","doi":"10.1007/s11274-024-04130-8","DOIUrl":"https://doi.org/10.1007/s11274-024-04130-8","url":null,"abstract":"<p><i>Euglena gracilis</i> is a unique microalga that lacks a cell wall and is able to grow under different trophic culture conditions. In this study, cell growth, biomass production, and changes in the ultrastructure of <i>E. gracilis</i> cells cultivated photoautotrophically, mixotrophically, and under sequential-heterotrophy-photoinduction (SHP) were assessed. Mixotrophy induced the highest cell growth and biomass productivity (6.27 ± 0.59 mg/L/d) in <i>E. gracilis</i>, while the highest content of fatty acids, 2.69 ± 0.04% of dry cell weight (DCW) and amino acids, 38.16 ± 0.08% of DCW was obtained under SHP condition. <i>E. gracilis</i> also accumulated significantly higher saturated fatty acids and lower unsaturated fatty acids when cultivated under SHP condition. Transcriptomic analysis showed that the expression of photosynthetic genes (<i>PsbA</i>,<i> PsbC</i>, F-type ATPase alpha and beta) was lower, carbohydrate and protein synthetic genes (<i>glnA</i>,<i> alg14</i> and <i>fba</i>) were expressed higher in SHP-culture cells when compared to other groups. Different trophic conditions also induced changes in the cell ultrastructure, where paramylon and starch granules were more abundant in SHP-cultured cells. The findings generated in this study illustrated that aerobic SHP cultivation of <i>E. gracilis</i> possesses great potential in human and animal feed applications.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The divergence of DHN-derived melanin pathways in Metarhizium robertsii","authors":"Linan Xie, Yang Liu, Yujie Zhang, Kang Chen, Qun Yue, Chen Wang, Baoqing Dun, Yuquan Xu, Liwen Zhang","doi":"10.1007/s11274-024-04134-4","DOIUrl":"https://doi.org/10.1007/s11274-024-04134-4","url":null,"abstract":"<p>The important role of dihydroxynaphthalene-(DHN) melanin in enhancing fungal stress resistance and its importance in fungal development and pathogenicity are well-established. This melanin also aids biocontrol fungi in surviving in the environment and effectively infecting insects. However, the biosynthetic origin of melanin in the biocontrol agents, <i>Metarhizium</i> spp., has remained elusive due to the complexity resulting from the divergence of two DHN-like biosynthetic pathways. Through the heterologous expression of biosynthetic enzymes from these two pathways in baker’s yeast <i>Saccharomyces cerevisiae</i>, we have confirmed the presence of DHN biosynthesis in <i>M. roberstii</i>, and discovered a novel naphthopyrone intermediate, <b>8</b>, that can produce a different type of pigment. These two pigment biosynthetic pathways differ in terms of polyketide intermediate structures and subsequent modification steps. Stress resistance studies using recombinant yeast cells have demonstrated that both DHN and its intermediates confer resistance against UV light prior to polymerization; a similar result was observed for its naphthopyrone counterpart. This study contributes to the understanding of the intricate and diverse biosynthetic mechanisms of fungal melanin and has the potential to enhance the application efficiency of biocontrol fungi such as <i>Metarhizium</i> spp. in agriculture.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive physiological and metabolic alterations in Staphylococcus aureus evolution under vancomycin exposure","authors":"Xin Cheng, Yue Shi, Yadong Liu, Yibin Xu, Jingxin Ma, Liyan Ma, Zerui Wang, Shuilong Guo, Jianrong Su","doi":"10.1007/s11274-024-04128-2","DOIUrl":"https://doi.org/10.1007/s11274-024-04128-2","url":null,"abstract":"<p><i>Staphylococcus aureus</i> can develop antibiotic resistance and evade immune responses, causing infections in different body sites. However, the metabolic changes underlying this process are poorly understood. A variant strain, C1V, was derived from the parental strain C1 by exposing it to increasing concentrations of vancomycin in vitro. C1V exhibited a vancomycin-intermediate phenotype and physiological changes compared to C1. It showed higher survival rates than C1 when phagocytosed by Raw264.7 cells. Metabolomics analysis identified significant metabolic differences pre- and post-induction (C1 + SC1 vs. C1V + SC1V: 201 metabolites) as well as pre- and post-phagocytosis (C1 vs. SC1: 50 metabolites; C1V vs. SC1V: 95 metabolites). The variant strain had distinct morphological characteristics, decreased adhesion ability, impaired virulence, and enhanced resistance to phagocytosis compared to the parental strain. Differential metabolites may contribute to <i>S. aureus</i> ‘ resistance to antibiotics and phagocytosis, offering insights into potential strategies for altering vancomycin nonsusceptibility and enhancing phagocyte killing by manipulating bacterial metabolism.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of laccase on mesoporous metal organic frameworks for efficient cross-coupling of ethyl ferulate","authors":"Xinqi Xu, Feng Shen, Gan Lv, Juan Lin","doi":"10.1007/s11274-024-04125-5","DOIUrl":"https://doi.org/10.1007/s11274-024-04125-5","url":null,"abstract":"<p>Laccases act as green catalysts for oxidative cross-coupling of phenolic antioxidnt compounds, but low stability and non-recyclability limit its application. To address that, metal–organic frameworks Cu-BTC and Cr-MOF were synthesized as supports to immobilize the efficient laccase from <i>Cerrena</i> sp. HYB07. The Brunauer–Emmett–Teller surface area of Cu-BTC and Cr-MOF were 1213.2 and 907.1 m²/g, respectively. The two carriers respectively presented pore diameters of 1.2–10 nm and 1.4–12 nm as octahedron, indicating nano-scale mesoporosity. These Cu-BTC and Cr-MOF carriers could adsorb laccase with enzyme loading of 1933.2 and 1564.4 U/g carrier, respectively. The stability and organic solvent tolerance of Cu-BTC-laccase and Cr-MOF-laccase were both obviously improved compared to free laccase. Thermal inactivation kinetics showed that both the two immobilized laccases displayed lower thermal inactivation rate constants. Importantly, the Cu-BTC-laccase and Cr-MOF-laccase both showed much higher activity for cross-coupling of ethyl ferulate than free laccase, which had 2.5-fold higher cross-coupling efficiency than that by free laccase. The ethyl ferulate coupling product was also analyzed by mass spectroscopy and the synthesis pathway of ethyl ferulate dimer was proposed. The cross coupling of ethyl ferulate required the formation of radical intermediates of ethyl ferulate generated by laccase mediated oxidation. This work paved the way for MOFs immobilized laccase for cross coupling of antioxidant phenols.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding microbial biomineralization at the molecular level: recent advances","authors":"Ankita Debnath, Sayak Mitra, Supratit Ghosh, Ramkrishna Sen","doi":"10.1007/s11274-024-04132-6","DOIUrl":"https://doi.org/10.1007/s11274-024-04132-6","url":null,"abstract":"<p>Microbial biomineralization is a phenomenon involving deposition of inorganic minerals inside or around microbial cells as a direct consequence of biogeochemical cycling. The microbial metabolic processes often create environmental conditions conducive for the precipitation of silicate, carbonate or phosphate, ferrate forms of ubiquitous inorganic ions. Till date the fundamental mechanisms underpinning two of the major types of microbial biomineralization such as, microbially controlled and microbially induced remains poorly understood. While microbially-controlled mineralization (MCM) depends entirely on the genetic makeup of the cell, microbially-induced mineralization (MIM) is dependent on factors such as cell morphology, cell surface structures and extracellular polymeric substances (EPS). In recent years, the organic template-mediated nucleation of inorganic minerals has been considered as an underlying mechanism based on the principles of solid-state bioinorganic chemistry. The present review thus attempts to provide a comprehensive and critical overview on the recent progress in holistic understanding of both MCM and MIM, which involves, organic–inorganic biomolecular interactions that lead to template formation, biomineral nucleation and crystallization. Also, the operation of specific metabolic pathways and molecular operons in directing microbial biomineralization have been discussed. Unravelling these molecular mechanisms of biomineralization can help in the biomimetic synthesis of minerals for potential therapeutic applications, and facilitating the engineering of microorganisms for commercial production of biominerals.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yarrowia lipolytica growth, lipids, and protease production in medium with higher alkanes and alkenes","authors":"Sílvia M. Miranda, Isabel Belo, Marlene Lopes","doi":"10.1007/s11274-024-04123-7","DOIUrl":"https://doi.org/10.1007/s11274-024-04123-7","url":null,"abstract":"<p>Two strains of <i>Yarrowia lipolytica</i> (CBS 2075 and DSM 8218) were first studied in bioreactor batch cultures, under different controlled dissolved oxygen concentrations (DOC), to assess their ability to assimilate aliphatic hydrocarbons (HC) as a carbon source in a mixture containing 2 g·L⁻¹ of each alkane (dodecane and hexadecane), and 2 g·L⁻¹ hexadecene. Both strains grew in the HC mixture without a lag phase, and for both strains, 30 % DOC was sufficient to reach the maximum values of biomass and lipids. To enhance lipid-rich biomass and enzyme production, a pulse fed-batch strategy was tested, for the first time, with the addition of one or three pulses of concentrated HC medium. The addition of three pulses of the HC mixture (total of 24 g·L⁻¹ HC) did not hinder cell proliferation, and high protease (&gt; 3000 U·L⁻¹) and lipids concentrations of 3.4 g·L⁻¹ and 4.3 g·L⁻¹ were achieved in <i>Y. lipolytica</i> CBS 2075 and DSM 8218 cultures, respectively. Lipids from the CBS 2075 strain are rich in C16:0 and C18:1, resembling the composition of palm oil, considered suitable for the biodiesel industry. Lipids from the DSM 8218 strain were predominantly composed of C16:0 and C16:1, the latter being a valuable monounsaturated fatty acid used in the pharmaceutical industry. <i>Y. lipolytica</i> cells exhibited high intrinsic surface hydrophobicity (&gt; 69 %), which increased in the presence of HC. A reduction in surface tension was observed in both <i>Y. lipolytica</i> cultures, suggesting the production of extracellular biosurfactants, even at low amounts. This study marks a significant advancement in the valorization of HC for producing high-value products by exploring the hydrophobic compounds metabolism of <i>Y. lipolytica</i>.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142221285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced production of health-promoting phenolic compounds using a novel endophytic fungus Talaromyces neorugulosus R-209 isolated from pigeon pea in a natural habitat by l-phenylalanine feeding","authors":"Jin-Xian Fu, Jiao Jiao, Qing-Yan Gai, Yu-Jie Fu, Jie Gao, Zi-Yi Zhang, Yuan Wang, Xiao-Qing Wang","doi":"10.1007/s11274-024-04122-8","DOIUrl":"https://doi.org/10.1007/s11274-024-04122-8","url":null,"abstract":"<p>In this study, nine endophytic fungi capable of producing multiple phenolic compounds were screened and identified from 152 fungi isolated from pigeon pea in a natural habitat (Honghe, Yunnan Province, China). <i>Talaromyces neorugulosus</i> R-209 exhibited the highest potential for phenolic compound production. L-phenylalanine feeding was used to enhance phenolic compound production in <i>T. neorugulosus</i> R-209 cultures. Under the optimal feeding conditions (l-phenylalanine dose of 0.16 g/L and feeding phase of 6 days), the yields of genistein, apigenin, biochanin A, and cajaninstilbene acid increased by 15.59-fold, 7.20-fold, 25.93-fold, and 10.30-fold over control, respectively. <i>T. neorugulosus</i> R-209 fed with l-phenylalanine was found to be stable in the production of phenolic compounds during ten successive subcultures. Moreover, bioactivities of extracts of <i>T. neorugulosus</i> R-209 cultures were significantly increased by l-phenylalanine feeding. Overall, l-phenylalanine feeding strategy made <i>T. neorugulosus</i> R-209 more attractive as a promising alternative source for the production of health-beneficial phenolic compounds in the nutraceutical/medicinal industries.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"183 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142221283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nisin-inducible chromosomal gene expression system based on ICE Tn5253 of Streptococcus pneumoniae, transferable among streptococci and enterococci","authors":"Mariana Tirziu, Lorenzo Colombini, Maria Alfreda Stincarelli, Anna Maria Cuppone, Elisa Lazzeri, Francesco Santoro, Gianni Pozzi, Francesco lannelli","doi":"10.1007/s11274-024-04124-6","DOIUrl":"https://doi.org/10.1007/s11274-024-04124-6","url":null,"abstract":"<p>The present work reports the development and validation of a chromosomal expression system in <i>Streptococcus pneumoniae</i> which permits gene expression under the control of <i>Lactococcus lactis</i> lantibiotic nisin. The system is based on the integrative and conjugative element (ICE) Tn<i>5253</i> of <i>S. pneumoniae</i> capable of site-specific chromosomal integration and conjugal transfer to a variety of bacterial species. We constructed an insertion vector that integrates in Tn<i>5251</i>, an ICE contained in Tn<i>5253</i>, which carries the tetracycline resistance <i>tet</i>(M) gene. The vector contains the <i>nisRK</i> regulatory system operon, the <i>L. lactis</i> nisin inducible promoter P<i>nisA</i> upstream of a multiple cloning site for target DNA insertion, and is flanked by two DNA regions of Tn<i>5251</i> which drive homologous recombination in ICE Tn<i>5253</i>. For system evaluation, the <i>emm6.1::ha1</i> fusion gene was cloned and integrated into the chromosome of the Tn<i>5253</i>-carrying pneumococcal strain FR24 by transformation. This gene encodes a fusion protein containing the signal peptide, the 122 N-terminal and the 140 C-terminal aa of the <i>Streptococcus pyogenes</i> M6 surface protein joined to the HA1 subunit of the influenza virus A hemagglutinin. Quantitative RT-PCR analysis carried out on total RNA purified from nisin treated and untreated cultures showed an increase in <i>emm6.1::ha1</i> transcript copy number with growing nisin concentration. The expression of M6-HA1 protein was detected by Western blot and quantified by Dot blot, while Flow cytometry analysis confirmed the presence on the pneumococcal surface. Recombinant ICE Tn<i>5253</i>::[<i>nisRK</i>]-[<i>emm6.1::ha1</i>] containing the nisin-inducible expression system was successfully transferred by conjugation in different streptococcal species including <i>Streptococcus gordonii</i>, <i>S. pyogenes</i>,<i> Streptococcus agalactiae</i> and <i>Enterococcus faecalis</i>. As for <i>S. pneumoniae</i>, the <i>emm6.1::ha1</i> transcript copy number and the amount of M6-HA1 protein produced correlated with the nisin concentration used for induction in all investigated bacterial hosts. We demonstrated that this host-vector expression system is stably integrated as a single copy within the bacterial chromosome, is transferable to both transformable and non transformable bacterial species, and allows fine tuning of protein expression modulated by nisin concentration. These characteristics make our system suitable for a wide range of applications including complementation assays, physiological studies, host-pathogen interaction studies.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142221286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin-coated zinc oxide nanoparticles: a promising antivirulence formulation against pathogenic bacteria","authors":"Fatemeh Azizi Alidoust, Behnam Rasti, Hojjatolah Zamani, Mirsasan Mirpour, Amir Mirzaie","doi":"10.1007/s11274-024-03984-2","DOIUrl":"https://doi.org/10.1007/s11274-024-03984-2","url":null,"abstract":"<p>The use of engineered nanoparticles against pathogenic bacteria has gained attention. In this study, zinc oxide nanoparticles conjugated with rutin were synthesized and their antivirulence properties against <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>. The physicochemical characteristics of ZnO-Rutin NPs were investigated using SEM, FT-IR, XRD, DLS, EDS, and zeta potential analyses. Antimicrobial properties were evaluated by well diffusion, microdilution, growth curve, and hemolytic activity assays. The expression of quorum sensing (QS) genes including the <i>lasI</i> and <i>rhlI</i> in <i>P. aeruginosa</i> and <i>agrA</i> in <i>S. aureus</i> was assessed using real-time PCR. Swimming, swarming, twitching, and pyocyanin production by <i>P. aeruginosa</i> were evaluated. The NPs were amorphous, 14–100 nm in diameter, surface charge of -34.3 mV, and an average hydrodynamic size of 161.7 nm. Regarding the antibacterial activity, ZnO-Rutin NPs were more potent than ZnO NPs and rutin, and stronger inhibitory effects were observed on <i>S. aureus</i> than on <i>P. aeruginosa</i>. ZnO-Rutin NPs inhibited the hemolytic activity of <i>P. aeruginosa</i> and <i>S. aureus</i> by 93.4 and 92.2%, respectively, which was more efficient than bare ZnO NPs and rutin. ZnO-Rutin NPs reduced the expression of the <i>lasI</i> and <i>rhlI</i> in <i>P. aeruginosa</i> by 0.17–0.43 and 0.37–0.70 folds, respectively while the expression of the <i>agrA</i> gene in <i>S. aureus</i> was decreased by 0.46–0.56 folds. Furthermore, ZnO-Rutin NPs significantly reduced the swimming and twitching motility and pyocyanin production of <i>P. aeruginosa</i>. This study demonstrates the antivirulence features of ZnO-Rutin NPs against pathogenic bacteria which can be associated with their QS inhibitory effects.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}