World Journal of Microbiology and Biotechnology最新文献_第10页

Entomopathogenic fungi tested in planta on pepper and in field on sorghum, to control commercially important species of aphids. 昆虫病原真菌在辣椒和高粱上的植物试验，以控制商业上重要的蚜虫种类。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-05 DOI: 10.1007/s11274-022-03268-7

Spiridon Mantzoukas, Patricia Tamez-Guerra, Francisco Zavala-Garcia, Ioannis Lagogiannis, Maria Julissa Ek-Ramos

{"title":"Entomopathogenic fungi tested in planta on pepper and in field on sorghum, to control commercially important species of aphids.","authors":"Spiridon Mantzoukas, Patricia Tamez-Guerra, Francisco Zavala-Garcia, Ioannis Lagogiannis, Maria Julissa Ek-Ramos","doi":"10.1007/s11274-022-03268-7","DOIUrl":"10.1007/s11274-022-03268-7","url":null,"abstract":"Given the aphids high reproductive capacity, assessing their biocontrol by using entomopathogenic fungi is crucial; to determine their potential, fungi were tested in planta and in field conditions. Significant decrease of Myzus persicae (Sulzer) population was observed in planta after applying Beauveria bassiana (strain 7R), Trichoderma gamsii (strain Z) or Metarhizium brunneum (strain Meta Br1) at 1 × 107 or 1 × 108 conidia/mL on pepper plants. Significant differences of aphids' populations were detected between fungus concentration and control (F = 68.743, df = 6.980, P < 0.001), where M. brunneum at 1 × 108 conidia/mL reduced aphids population close to zero. At 20 °C, dead aphids' mycosis by B. bassiana and T. gamsii was 78% and 84%; at 25 °C was 83% and 88%; and at 30 °C was 75% and 79%, respectively. In field conditions, Mexican PTG4 and commercial GHA B. bassiana strains were tested [(1 × 106 conidia/mL + corn starch) seed treatments] against the Melanaphis sacchari (Zehntner) aphid populations, on naturally infested sorghum plants. Results showed that plant germination and emergence were not affected, whereas yield (grams of sugar/plant) was significantly higher among treated compared with untreated plants. The aphid population decreased in plants from PTG4 treated seeds; indeed, this treatment had a significant positive effect on the flowering index, whereas the stem fresh weight and juice volume was significantly increased among plants from GHA treated seeds. Taken together, tested strains can be used as a tool to control aphids' population on several crops such as pepper and even increase the yield in sorghum.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"2 1","pages":"84"},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76241535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron transfer in Gram-positive bacteria: enhancement strategies for bioelectrochemical applications. 革兰氏阳性细菌中的电子转移：生物电化学应用的增强策略。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-30 DOI: 10.1007/s11274-022-03255-y

Ola M Gomaa, Nazua L Costa, Catarina M Paquete

{"title":"Electron transfer in Gram-positive bacteria: enhancement strategies for bioelectrochemical applications.","authors":"Ola M Gomaa, Nazua L Costa, Catarina M Paquete","doi":"10.1007/s11274-022-03255-y","DOIUrl":"10.1007/s11274-022-03255-y","url":null,"abstract":"To this day, bioelectrochemical systems are still perceived as one of the rising technologies due to their versatile applications in electricity production, bioremediation, biosensors, and production of value-added products. While the majority of bioelectrochemical applications utilize Gram-negative bacteria, Gram-positive bacteria has not received sufficient attention. The lack of adequate knowledge about their electron transfer pathways along with the presence of a thick non-conductive cell wall are among the reasons behind their limited use. In this review, the electroactivity of Gram-positive bacteria will be covered describing the different pathways of electron transfer among different electroactive Gram-positive strains. Special emphasis will be given to the role of multiheme cytochromes, quorum sensing molecules, peptide-based signalling, and pili in the extracellular electron transfer. This review will also provide an overview of possible approaches for enhancement strategies of electron transfer such as enhancing biofilm formation, biocomposites and cell perforation. Understanding the fundamentals is critical for improving the use of Gram-positive bacteria in bioelectrochemical systems and may lead to the discovery of new applications.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"5 1","pages":"83"},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78743517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production. 阻断肺炎克雷伯菌2,3-丁二醇合成途径导致l -缬氨酸的产生。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-29 DOI: 10.1007/s11274-022-03266-9

Qinghui Wang, Jinjie Gu, Lin Shu, Weiyan Jiang, Ljiljana Mojovic, Zorica Knezevic-Jugovic, Jiping Shi, Frank Baganz, Gary J Lye, Wensheng Xiang, Jian Hao

{"title":"Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production.","authors":"Qinghui Wang, Jinjie Gu, Lin Shu, Weiyan Jiang, Ljiljana Mojovic, Zorica Knezevic-Jugovic, Jiping Shi, Frank Baganz, Gary J Lye, Wensheng Xiang, Jian Hao","doi":"10.1007/s11274-022-03266-9","DOIUrl":"10.1007/s11274-022-03266-9","url":null,"abstract":"Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of L-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the L-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the L-valine synthesis. 2-Ketoisovalerate is the precursor of L-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of L-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of L-valine production. The brnQ encodes a branched-chain amino acid transporter, and L-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor L-valine production. Based on these findings, L-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of L-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"49 1","pages":"81"},"PeriodicalIF":0.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84956536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetotactic bacteria in vertical sediments of volcanic lakes in NE China appear Alphaproteobacteria dominated distribution regardless of waterbody types. 东北地区火山湖垂直沉积物中趋磁细菌不论水体类型均以α变形杆菌为主。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-19 DOI: 10.1007/s11274-022-03262-z

Tao Liu, Huiyun Da, Shuang Zhang, Weidong Wang, Hong Pan, Lei Yan

{"title":"Magnetotactic bacteria in vertical sediments of volcanic lakes in NE China appear Alphaproteobacteria dominated distribution regardless of waterbody types.","authors":"Tao Liu, Huiyun Da, Shuang Zhang, Weidong Wang, Hong Pan, Lei Yan","doi":"10.1007/s11274-022-03262-z","DOIUrl":"10.1007/s11274-022-03262-z","url":null,"abstract":"Magnetotactic bacteria (MTB) distribute widely in sediment habitats and play critical roles in iron cycling. Here, the vertical distribution of morphology and phylogenetic diversity of MTB in sediments (0-15 cm) of three lakes (open waterbody, Bailonghu, BL; semi-enclosed waterbody, Yaoquanhu, YQ; enclosed waterbody, Yueyapao, YY) in Wudalianchi volcanic field (China) were investigated. TEM showed the appearance of coccoid, rod-shaped, oval-shaped, and arc-shaped MTB. With the increase of BL sediment depth, the number of rod-shaped and spherical MTB decreased and increased, respectively. High-throughput sequencing indicated that Alphaproteobacterial MTB dominantly thrived in these lakes regardless of waterbody types. In BL and YY, the dominant genus was Magnetospirillum (44.99-70.80%) which showed a peak in the middle layer. In YQ, the genus Magnetospira was dominant in the upper (52.36%) and middle (66.56%) layer and Magnetococcus (69.63%) existed dominantly in the bottom layer. The vertical distribution of MTB in sediments of these lakes decreased first and then increased. Functional analysis showed that ABC transporter and two-component system of MTB changed significantly with the sediment depth. RDA indicated that the distribution of Magnetospirillum was positively associated with sulfide, pH, and TC. These findings will expand our knowledge of the vertical distribution of MTB in volcanic lakes.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"9 1","pages":"76"},"PeriodicalIF":0.0,"publicationDate":"2022-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89851701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms. 香菇担子果结构：一种抗食源性病原体和食品腐败微生物的抗菌来源。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-17 DOI: 10.1007/s11274-022-03257-w

Míria Benetati Delgado Bertéli, Mariana Martins Magalhães de Souza, Lillian Barros, Isabel C F R Ferreira, Jasmina Glamočlija, Marina Soković, Douglas Cardoso Dragunski, Juliana Silveira do Valle, Ederlan de Souza Ferreira, Laise Cedraz Pinto, Carolina Oliveira de Souza, Suelen Pereira Ruiz, Giani Andrea Linde, Nelson Barros Colauto

{"title":"Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms.","authors":"Míria Benetati Delgado Bertéli, Mariana Martins Magalhães de Souza, Lillian Barros, Isabel C F R Ferreira, Jasmina Glamočlija, Marina Soković, Douglas Cardoso Dragunski, Juliana Silveira do Valle, Ederlan de Souza Ferreira, Laise Cedraz Pinto, Carolina Oliveira de Souza, Suelen Pereira Ruiz, Giani Andrea Linde, Nelson Barros Colauto","doi":"10.1007/s11274-022-03257-w","DOIUrl":"10.1007/s11274-022-03257-w","url":null,"abstract":"Lentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL- 1, for streptomycin from 0.10 to 0.50 mg mL- 1, and for ampicillin from 0.40 to 1.20 mg mL- 1. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL- 1, for bifonazole from 0.20 to 0.25 mg mL- 1, and for ketoconazole from 0.30 to 3.50 mg mL- 1. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"21 1","pages":"74"},"PeriodicalIF":0.0,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89853202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and mutagenesis of a novel Mycobacterium smegmatis-derived glutamate decarboxylase active at neutral pH. 中性pH下活性的新型耻垢分枝杆菌衍生谷氨酸脱羧酶的鉴定和诱变。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-17 DOI: 10.1007/s11274-022-03252-1

Yudi Li, Guiying Chen, Fanglan Ge, Ting Dang, Yao Ren, Bei Zeng, Wei Li

{"title":"Characterization and mutagenesis of a novel Mycobacterium smegmatis-derived glutamate decarboxylase active at neutral pH.","authors":"Yudi Li, Guiying Chen, Fanglan Ge, Ting Dang, Yao Ren, Bei Zeng, Wei Li","doi":"10.1007/s11274-022-03252-1","DOIUrl":"10.1007/s11274-022-03252-1","url":null,"abstract":"γ-aminobutyric acid (GABA) has various physiological functions and is widely used in medicine, food, and other fields. Glutamate decarboxylase (GAD) is a key enzyme that catalyzes the decarboxylation of L-glutamate to synthesize GABA. However, the industrial application of microorganism-derived GAD is limited by its rapid loss of enzymatic activity with pH approaching neutrality. In this study, a novel glutamate decarboxylase, GADMSM, from Mycobacterium smegmatis was overexpressed and purified. On the basis of homologous modeling and substrate molecular docking, several GADMSM mutants were constructed, and their enzymatic properties were analyzed. The results showed that the optimal pH of wild-type GADMSM is 5.4; at pH 6.2, 22.8% enzymatic activity was retained. The T211I replacement in GAD and C-terminal deletion mutant GADMSMΔC showed relatively high catalytic activity in a pH range of 5.0-7.0. The Vmax and Km values of GADMSMΔC were 14.69 and 5.70, respectively, at pH 5.5, and 9.87 and 6.17, respectively, at pH 7.0. Compared with the wild-type GAD, GADMSMΔC maintained higher affinity and enzymatic activity of the substrate, maintaining 78.5% of the highest enzymatic activity even at pH 7.0, which is the highest reported activity retention for GAD under neutral pH condition. Therefore, GADMSMΔC can be used for the transformation of high-yielding strains and industrial production of GABA.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"18 1","pages":"75"},"PeriodicalIF":0.0,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90182973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-grafted-phenolic acid copolymers against Shewanella putrefaciens by disrupting the permeability of cell membrane. 壳聚糖接枝酚醛酸共聚物通过破坏细胞膜的通透性来抵抗腐烂希瓦氏菌。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-15 DOI: 10.1007/s11274-022-03261-0

Weiqing Lan, Jiaxin Zhao, Yuqing Sun, Jiali Liu, Jing Xie

{"title":"Chitosan-grafted-phenolic acid copolymers against Shewanella putrefaciens by disrupting the permeability of cell membrane.","authors":"Weiqing Lan, Jiaxin Zhao, Yuqing Sun, Jiali Liu, Jing Xie","doi":"10.1007/s11274-022-03261-0","DOIUrl":"10.1007/s11274-022-03261-0","url":null,"abstract":"Chitosan (CS) is a kind of high molecular polymer with antibacterial properties. A copolymer with high bacteriostatic activity can be formed by grafting phenolic acid compounds into the chitosan molecular chain, which can inhibit the growth of dominant spoilage bacteria in aquatic products. The study aimed to investigate the antibacterial effect and mechanism of chitosan-grafted-phenolic acid copolymers on Shewanella putrefaciens (S. putrefaciens). CS-grafted-protocatechuic acid (CS-g-PA) and CS-grafted-gallic acid (CS-g-GA) were attained by EDC/NHS coupling reaction. The antibacterial tests indicated that CS-g-PA and CS-g-GA had the same minimum inhibitory concentration (MIC) (1.25 mg/mL) and minimum bactericidal concentration (MBC) (5.0 mg/mL) against S. putrefaciens. According to the change trend of growth curve, the growth of S. putrefaciens was significantly restrained under 2MIC graft copolymers (P < 0.05). Moreover, the increment of alkaline phosphatase (AKPase) activity and electrical conductivity demonstrated that the cell wall and membrane permeability of S. putrefaciens were damaged respectively. In addition, the increase of lactate dehydrogenase (LDHase) activity, protein and nucleic acid absorbance and the decrease of adenosine triphosphatase (ATPase) activity suggested that the cell membrane was incomplete and poor fluidity. The irregular shape of bacteria and the outflow of intercellular contents were also observed from scanning electron microscope (SEM). The above results manifested a great potential of CS-g-PA and CS-g-GA for use as food preservatives to aquatic products.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"49 1","pages":"73"},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73775258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique bacterial communities associated with components of an artificial aquarium ecosystem and their possible contributions to nutrient cycling in this microecosystem. 与人工水族生态系统组成部分相关的独特细菌群落及其对该微生态系统中营养循环的可能贡献。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-12 DOI: 10.1007/s11274-022-03258-9

Jun Shi Zhou, Jian Fei Cheng, Xue Dong Li, Yan Hong Li

{"title":"Unique bacterial communities associated with components of an artificial aquarium ecosystem and their possible contributions to nutrient cycling in this microecosystem.","authors":"Jun Shi Zhou, Jian Fei Cheng, Xue Dong Li, Yan Hong Li","doi":"10.1007/s11274-022-03258-9","DOIUrl":"10.1007/s11274-022-03258-9","url":null,"abstract":"In order to better understand the bacterial distribution characteristics in a whole microecosystem, the bacterial communities in different components of an artificial aquarium (i.e., plants, fishes, sand and water) were characterized using high throughput sequencing of bacterial 16S rRNA genes. Across all samples, 2873 operational taxonomic units were identified and assigned to 771 genera in 36 phyla. In a principle coordinate analysis, samples clustered according to their origin, indicating that bacterial communities from the same component were most similar. Further taxonomic analysis revealed that most dominant genera, even those with the similar functions, were biased to one component: Nitrospira and Rhodobacter were mainly abundant in plant samples; Rhodococcus, Serratia, Ralstonia, Sphingobacterium and Pseudomonas were most common in sand samples; Cetobacterium and Aeromonas dominated fish samples; and Flavobacterium, Alpinimonas and Limnobacter were especially common in water samples. Functional predictions performed by PICRUSt and the dominant genera exhibited that bacteria detected in each component could participate in all nutrient cycles in the aquarium. However, those involved in carbon and nitrogen cycling were most common in plant and fish samples, while phosphate metabolism-related pathways were more abundant in sand and water samples. Moreover, the aquarium plants, in association with their bacterial communities might be the most important component in the aquarium, as indicated by their highest bacterial richness and diversity. This study adds to our understanding on the differences in the microbiome of different components and their possible contributions to nutrient cycling in a self-sustaining aquarium.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"2 1","pages":"72"},"PeriodicalIF":0.0,"publicationDate":"2022-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91367527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bright as day and dark as night: light-dependant energy for lipid biosynthesis and production in microalgae. 亮如昼暗如夜：微藻脂质合成和生产的光依赖性能量。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-08 DOI: 10.1007/s11274-022-03245-0

Cher Chien Lau, Kit Yinn Teh, C L Wan Afifudeen, Willy Yee, Ahmad Aziz, Thye San Cha

{"title":"Bright as day and dark as night: light-dependant energy for lipid biosynthesis and production in microalgae.","authors":"Cher Chien Lau, Kit Yinn Teh, C L Wan Afifudeen, Willy Yee, Ahmad Aziz, Thye San Cha","doi":"10.1007/s11274-022-03245-0","DOIUrl":"10.1007/s11274-022-03245-0","url":null,"abstract":"Microalgae are photosynthetic organisms functioning as the green bio-factories for various pharmaceutical and biofuel products. To date, numerous attempts have been carried out to manipulate culture conditions to maximize the production of the desired metabolites. Because light is the energy source of microalgae for their growth and metabolites biosynthesis, it has been one of the most investigated variables emphasized on the deep understanding of how microalgae respond towards light changes as an external stimulus. This review discusses the effects of different light sources, light intensities, light wavelengths and length of photoperiod on various microalgae species, especially in terms of biomass and lipid productivity. Additionally, the relationship between photoregulation processes and lipid productivity of microalgae are also deliberated. The current available approaches of microalgae mass cultivation, including different types of open and closed systems are recapitulated with the intention to highlight the significant insights for the design of future photoreactors.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"38 1","pages":"70"},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72946029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of transcription activator-like effectors and pathogenicity in strains of Xanthomonas oryzae pv. oryzicola from Yunnan. 水稻黄单胞菌转录激活因子样效应物的多样性及其致病性。来自云南的米紫甘蓝。

World Journal of Microbiology and Biotechnology Pub Date : 2022-03-08 DOI: 10.1007/s11274-022-03230-7

Jun Yang, Xiaofang Zhang, Shahzad Munir, Xinli Miao, Lihong Zhou, Qi Liu, Jinhao Zhang, Xiaoyan Wan, Lanfang Wei, Guanghai Ji

{"title":"Diversity of transcription activator-like effectors and pathogenicity in strains of Xanthomonas oryzae pv. oryzicola from Yunnan.","authors":"Jun Yang, Xiaofang Zhang, Shahzad Munir, Xinli Miao, Lihong Zhou, Qi Liu, Jinhao Zhang, Xiaoyan Wan, Lanfang Wei, Guanghai Ji","doi":"10.1007/s11274-022-03230-7","DOIUrl":"10.1007/s11274-022-03230-7","url":null,"abstract":"The present study aimed to evaluate transcriptional activator-like effector (TALE) genes in 86 Xanthomonas oryzae pv. oryzicola strains collected from 8 rice-growing regions in Yunnan, and to examine the relationship between TALE genotypes and virulence in 6 differential rice lines. Besides, the geographical areas, distribution of these genotypes were studied in detail. Genetic diversity was analyzed through the number and size of putative TALE genes based on TALE gene avrXa3 as a probe. We found that X. oryzae pv. oryzicola strains consist of variable number (13-27) of avrXa3-hybridizing fragments (putative TALE genes). Test strains were classified into 8 genotypes (G1-G8) with major genotypes G3 and G7 widely distributed in Yunnan. Pathogenicity of X. oryzae pv. oryzicola was evaluated by inoculating 6 differential rice lines with a single resistance gene into 9 pathotypes clusters (I-IX), the dominant Genotypes G3 and G7 consist of pathotypes I, II, and IV. Furthermore, we also detected the known TALE target genes expression in susceptible rice cultivar (cv. nipponbare) after inoculating 8 genotypes-representative X. oryzae pv. oryzicola strain. Correlation between the numbers of putative TALE genes of X. oryzae pv. oryzicola and relevant target genes in nipponbare confirmed up-regulation. Altogether, this study has given insights into the population structure of X. oryzae pv. oryzicola that may inform strategies to control BLS in rice.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"34 2 1","pages":"71"},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77979609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0