World Journal of Microbiology and Biotechnology最新文献_第9页

Nutritional influences on biomass behaviour and metabolic products by Chlamydomonas reinhardtii. 营养对莱茵衣藻生物量行为和代谢产物的影响。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-23 DOI: 10.1007/s11274-022-03277-6

Letícia de M Sousa, Juliana de S Ferreira, Vicelma L Cardoso, Fabiana R X Batista

引用次数: 0

Endophytic fungi of Brunfelsia uniflora: isolation, cryopreservation, and determination of enzymatic and antioxidant activity. 独花褐藻内生真菌的分离、低温保存及酶和抗氧化活性的测定。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-20 DOI: 10.1007/s11274-022-03278-5

Sara Jane Marsola, Lais Freitas Jorge, Adriano Borges Meniqueti, Miria Benetati Delgado Bertéli, Thaís Emanuelle Feijó de Lima, José Luiz Bezerra, Ana Daniela Lopes, Zilda Cristiani Gazim, Juliana Silveira do Valle, Nelson Barros Colauto, Giani Andrea Linde

{"title":"Endophytic fungi of Brunfelsia uniflora: isolation, cryopreservation, and determination of enzymatic and antioxidant activity.","authors":"Sara Jane Marsola, Lais Freitas Jorge, Adriano Borges Meniqueti, Miria Benetati Delgado Bertéli, Thaís Emanuelle Feijó de Lima, José Luiz Bezerra, Ana Daniela Lopes, Zilda Cristiani Gazim, Juliana Silveira do Valle, Nelson Barros Colauto, Giani Andrea Linde","doi":"10.1007/s11274-022-03278-5","DOIUrl":"10.1007/s11274-022-03278-5","url":null,"abstract":"Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro, is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic, depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of Brunfelsia; however, few have focused on the diversity of endophytic microorganisms that colonize members of the genus. This study aimed to isolate and cryopreserve endophytic fungi from B. uniflora and determine their cellulase, laccase, and antioxidant activities. Endophytic fungi were isolated from B. uniflora stems, cultured on wheat grains, immersed in a 150 g L-1 aqueous sucrose solution, and cryopreserved at - 80 °C for 1 and 6 months. Cellulase activity was determined by a qualitative test using carboxymethylcellulose medium and laccase activity by a quantitative test based on the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Prior to antioxidant activity assays, fungi were grown in malt extract broth for production of mycelial biomass. A methanolic extract was prepared for evaluation of DPPH· scavenging activity, FRAP activity, and total phenolic content. A total of 46 endophytic fungal isolates were obtained from B. uniflora stems and classified into 24 groups according to morphological similarities. B. uniflora was shown to harbor different genera of ascomycete fungi as endophytic organisms. Mycelial viability was observed after 1 and 6 months of cryopreservation at - 80 °C. Fungi exhibited cellulase and laccase activities. Isolate CE23 had the highest laccase activity after 7 days of cultivation. Twelve isolates were found to have low total phenolic contents and DPPH· and FRAP activities.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"02 1","pages":"94"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80103638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in strategies for steroid production in yeasts. 酵母中类固醇生产策略的最新进展。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-20 DOI: 10.1007/s11274-022-03276-7

Yi-Qi Jiang, Jian-Ping Lin

引用次数: 0

Protein potential of Desmodesmus asymmetricus grown in greenhouse as an alternative food source for aquaculture. 温室非对称桥丝霉作为水产养殖替代食物来源的蛋白质潜力。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-16 DOI: 10.1007/s11274-022-03275-8

Alberto Oscanoa, Miguel Cervantes, Leenin Flores, Anthony Ruiz

{"title":"Protein potential of Desmodesmus asymmetricus grown in greenhouse as an alternative food source for aquaculture.","authors":"Alberto Oscanoa, Miguel Cervantes, Leenin Flores, Anthony Ruiz","doi":"10.1007/s11274-022-03275-8","DOIUrl":"10.1007/s11274-022-03275-8","url":null,"abstract":"During ten months, batch culture of Desmodesmus asymmetricus microalgae was carried out under greenhouse conditions. The inoculation ratio was 1:1 (inoculum:treated water). The cultures were maintained for 5 days with natural light and constant aeration mixed with carbon dioxide. The biomass was concentrated by centrifugation and dried by lyophilization; subsequently, total proteins and amino acid concentration were quantified. A relationship between biomass production and seasonal variation was observed, the lowest dry biomass production was recorded in June (38.8 ± 1.0 mg L-1 day-1) and July (43.3 ± 0.1 mg L-1 day-1); while the highest values were greater than 70 mg L-1 day-1 in March. There was a high positive correlation between wet and dry biomass (r = 0.97, p < 0.001) with a mean conversion of 26%. The mean percentage of protein was 26.1 ± 2.6%, the highest percentage was registered in March (31.03 ± 1.48%) as well as the concentration of amino acids. Regarding amino acids, arginine obtained the highest concentration (4.08 ± 0.43 g 100 g-1), followed by aspartic acid (3.36 ± 0.23 g 100 g-1), while the lowest values were for methionine (0.55 ± 0.21 g 100 g-1), histidine (0.77 ± 0.07 g 100 g-1) and tyrosine (1.01 ± 0.17 g 100 g-1). Finally, according to the essential amino acid index (in fish ≥ 0.90, in crustaceans > 0.80), the biomass of D. asymmetricus has potential as a food supplement for the production of feed in aquaculture.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"74 1","pages":"92"},"PeriodicalIF":0.0,"publicationDate":"2022-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86161769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial degradation and valorization of poly(ethylene terephthalate) (PET) monomers. 聚对苯二甲酸乙酯（PET）单体的微生物降解和增值。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-15 DOI: 10.1007/s11274-022-03270-z

Rui Gao, Haojie Pan, Lei Kai, Kun Han, Jiazhang Lian

{"title":"Microbial degradation and valorization of poly(ethylene terephthalate) (PET) monomers.","authors":"Rui Gao, Haojie Pan, Lei Kai, Kun Han, Jiazhang Lian","doi":"10.1007/s11274-022-03270-z","DOIUrl":"10.1007/s11274-022-03270-z","url":null,"abstract":"The polyethylene terephthalate (PET) is one of the major plastics with a huge annual production. Alongside with its mass production and wide applications, PET pollution is threatening and damaging the environment and human health. Although mechanical or chemical methods can deal with PET, the process suffers from high cost and the hydrolyzed monomers will cause secondary pollution. Discovery of plastic-degrading microbes and the corresponding enzymes emerges new hope to cope with this issue. Combined with synthetic biology and metabolic engineering, microbial cell factories not only provide a promising approach to degrade PET, but also enable the conversion of its monomers, ethylene glycol (EG) and terephthalic acid (TPA), into value-added compounds. In this way, PET wastes can be handled in environment-friendly and more potentially cost-effective processes. While PET hydrolases have been extensively reviewed, this review focuses on the microbes and metabolic pathways for the degradation of PET monomers. In addition, recent advances in the biotransformation of TPA and EG into value-added compounds are discussed in detail.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"91 26","pages":"89"},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91406958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of extracellular electron transfer in Pseudomonas aeruginosa by co-expression of intermediate genes in NAD synthetase production pathway. 利用NAD合成酶合成途径中间基因的共表达评价铜绿假单胞菌胞外电子转移。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-15 DOI: 10.1007/s11274-022-03274-9

Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Mrinalini Nair, Enrico Marsili, Abiodun Anthony Onilude

{"title":"Evaluation of extracellular electron transfer in Pseudomonas aeruginosa by co-expression of intermediate genes in NAD synthetase production pathway.","authors":"Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Mrinalini Nair, Enrico Marsili, Abiodun Anthony Onilude","doi":"10.1007/s11274-022-03274-9","DOIUrl":"10.1007/s11274-022-03274-9","url":null,"abstract":"Pseudomonas aeruginosa (PA) is an electrogenic bacterium, in which extracellular electron transfer (EET) is mediated by microbially-produced phenazines, especially pyocyanin. Increasing EET rate in electrogenic bacteria is key for the development of biosensors and bioelectrofermentation processes. In this work, the production of pyocyanin, Nicotinamide Adenine Dinucleotide (NAD) and NAD synthetase by the electrogenic strain PA-A4 is determined using a Microbial Fuel Cell (MFC). Effects of metabolic inhibition and enhancement of pyocyanin and NAD synthetase on NAD/NADH levels and electrogenicity was demonstrated by short chronoamperometry measurements (0-48 h). Combined overexpression of two intermediate NAD synthetase production genes-nicotinic acid mononucleotide adenyltransferase (nadD) and quinolic acid phosphoribosyltransferase (nadC) genes, which are distant on the PA genomic map, enabled co-transcription and increased NAD synthetase activity. The resulting PA-A4 nadD + nadC shows increases in pyocyanin concentration, NAD synthetase activity, NAD/NADH levels, and MFC potential, all significantly higher than its wild type. Extracellular respiratory mechanisms in PA are linked with NAD metabolism, and targeted increased yield of NAD could directly lead to enhanced EET. A previous attempt at enhancing NAD synthetase for electrogenicity by targeting the terminal NAD synthetase gene (nadE) in standard P. aeruginosa PA01 had earlier been reported. Our work however, poses another route to electrogenicity enhancement in PA using; a combination of nadD and nadC. Further experiments are needed to understand specific intracellular mechanisms governing how over-expression of nadD and nadC induced activity of NadE protein. These findings significantly advance the knowledge of the versatility of NAD biosynthetic genes in PA electrogenicity.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"57 1","pages":"90"},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74506434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol potential of endophytic Trichoderma citrinoviride HT-1 against root rot of Rheum palmatum through both antagonistic effects and induced systemic resistance. 内生真菌黄绿木霉HT-1对掌叶大黄根腐病的拮抗和诱导全身抗性的生物防治潜力。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-13 DOI: 10.1007/s11274-022-03272-x

DaWei Chen, QinZheng Hou, BaoLi Fan, Hui Zhang, LingYun Jia, Kun Sun

{"title":"Biocontrol potential of endophytic Trichoderma citrinoviride HT-1 against root rot of Rheum palmatum through both antagonistic effects and induced systemic resistance.","authors":"DaWei Chen, QinZheng Hou, BaoLi Fan, Hui Zhang, LingYun Jia, Kun Sun","doi":"10.1007/s11274-022-03272-x","DOIUrl":"10.1007/s11274-022-03272-x","url":null,"abstract":"Some Trichoderma species have been used as biocontrol agents in agriculture. The effectiveness of T. citrinoviride HT-1, a beneficial endophyte isolated from Rheum palmatum root was explored for control of root rot and its mechanisms of induced systemic resistance. The results showed that the inhibition rate of F. oxysporum was 71.85% in dual culture. The fermentation metabolites (FM) of the T. citrinoviride HT-1 strain suppressed mycelial growth of F. oxysporum, recording an inhibition rate of 79.07%. Conidial suspensions of T. citrinoviride HT-1(1 × 107 spores/mL) can suppress the root rot of R. palmatum caused by F. oxysporum to a low disease index (17.60) and had significant control effects on root rot (72.53%). The activities of induced defense-related enzymes in R. palmatum plants were significantly increased following T. citrinoviride HT-1 treatment. The RT-PCR analysis of the defense-related genes showed that T. citrinoviride HT-1 can increase the defense response-related gene expression. This study has contributed to our understanding of the biocontrol potential of T. citrinoviride HT-1 and provided a theoretical basis for the application as a bio-fungicide.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"29 1","pages":"88"},"PeriodicalIF":0.0,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81285672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial cellulose: recent progress in production and industrial applications. 细菌纤维素：生产和工业应用的最新进展。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-10 DOI: 10.1007/s11274-022-03271-y

Nermin Hande Avcioglu

{"title":"Bacterial cellulose: recent progress in production and industrial applications.","authors":"Nermin Hande Avcioglu","doi":"10.1007/s11274-022-03271-y","DOIUrl":"10.1007/s11274-022-03271-y","url":null,"abstract":"Bacterial cellulose (BC) is synthesized as a valuable extracellular biopolymer by several bacteria belonging to the genera of Acetobacter, Achromobacter, Komagataeibacter, Agrobacterium, Bacillus, Azotobacter, Sarcinia, Lactobacillus and Gluconacetobacter. Unlike plant cellulose, since BC does not contain lignin, hemicellulose, pectin, arabinose and other plant-derived contaminants, it can be obtained purely from the culture media without any purification processes. BC exhibits excellent physicochemical and mechanical properties such as purity, high crystallinity, transparency, porosity, high water holding capacity, ultrafine nanoscale fiber network, tensile strength, high degree of polymerization, high surface area, chemical stability and proton conductivity. In addition, BC has become an essential nanomaterial in many industrial processes as it is biocompatible, biodegradable and renewable. In this respect, researchers are focused on the production of BC using low-cost substrates, investigation of potential BC producers, optimization of cultivation conditions, and modification of BC pellicles with different procedures. Based on these researches, this review of recent progress in bacterial cellulose production, both in vivo and in vitro modifications of surface properties of BC and its industrial applications in different areas are discussed in this review.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"13 1","pages":"86"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88410605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression and characterization of two novel glucanases derived from sheep rumen microbiota. 来源于绵羊瘤胃微生物群的两种新型葡聚糖酶的异源表达和特性研究。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-10 DOI: 10.1007/s11274-022-03269-6

De-Ying Gao, Xiao-Bao Sun, Ying Fang, Bo He, Jun-Hong Wang, Jian-Xin Liu, Jia-Kun Wang, Qian Wang

{"title":"Heterologous expression and characterization of two novel glucanases derived from sheep rumen microbiota.","authors":"De-Ying Gao, Xiao-Bao Sun, Ying Fang, Bo He, Jun-Hong Wang, Jian-Xin Liu, Jia-Kun Wang, Qian Wang","doi":"10.1007/s11274-022-03269-6","DOIUrl":"10.1007/s11274-022-03269-6","url":null,"abstract":"β-Glucanases are a suite of glycoside hydrolases that depolymerize β-glucan into cellooligosaccharides and/or monosaccharides and have been widely used as feed additives in livestock. In this study, two novel glucanase genes, IDSGluc5-26 and IDSGluc5-37, derived from sheep rumen microbiota, were expressed and functionally characterized. The optimal temperatures/pH of recombinant IDSGLUC5-26 and IDSGLUC5-37 were 50 °C/5.0 and 40 °C/6.0, respectively. Notably, IDSGLUC5-26 showed considerable stability under acidic conditions. Both IDSGLUC5-26 and IDSGLUC5-37 showed the highest activities toward barley β-glucan, with Vmax values of 89.96 ± 9.19 µmol/min/mg and 459.50 ± 25.02 µmol/min/mg, respectively. Additionally, these two glucanases demonstrated hydrolysis of Icelandic moss lichenan and konjac gum, IDSGLUC5-26 releasing cellobiose (G2; occupying 17.37% of total reducing sugars), cellotriose (G3; 23.97%), and cellotetraose (G4; 30.93%) from barley β-glucan and Icelandic moss lichenan after 10 min and suggestive of a typical endo-β-1,4-glucanase (EC.3.2.1.4). In contrast, IDSGLUC5-37 was capable of liberating dominant G3 (64.11% or 67.55%) from barley β-glucan or Icelandic moss lichenan, suggesting that the enzyme was likely an endo-β-1,3 - 1,4-glucanases/lichenase (EC3.2.1.73). These findings describe the expression and characterization of two novel glucanase genes from sheep rumen microbiota. The two recombinant enzymes, particularly the acid-stable IDSGLUC5-26, will be of interest for potential application in food-/feed-additive development.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"39 1","pages":"87"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91388111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal-bacterial biofilm mediated heavy metal rhizo-remediation. 真菌-细菌生物膜介导的重金属根茎修复。

World Journal of Microbiology and Biotechnology Pub Date : 2022-04-05 DOI: 10.1007/s11274-022-03267-8

A P Henagamage, C M Peries, G Seneviratne

{"title":"Fungal-bacterial biofilm mediated heavy metal rhizo-remediation.","authors":"A P Henagamage, C M Peries, G Seneviratne","doi":"10.1007/s11274-022-03267-8","DOIUrl":"10.1007/s11274-022-03267-8","url":null,"abstract":"Heavy metal pollution due to excessive use of chemical fertilizers (CF) causes major damage to the environment. Microbial biofilms, closely associated with the rhizosphere can remediate heavy metal-contaminated soil by reducing plant toxicity. Thus, this study was undertaken to examine the remedial effects of microbial biofilms against contaminated heavy metals. Fungi and bacteria isolated from soil were screened for their tolerance against Cd2+, Pb2+, and Zn2+. Three bacterial and two fungal isolates were selected upon the tolerance index (TI) percentage. Fungal-bacterial biofilms (FBBs) were developed with the most tolerant isolates and were further screened for their bioremediation capabilities against heavy metals. The best biofilm was evaluated for its rhizoremediation capability with different CF combinations using a pot experiment conducted under greenhouse conditions with potatoes. Significantly (P < 0.05), the highest metal removal percentage was observed in Trichoderma harzianum and Bacillus subtilis biofilm under in situ conditions. When compared to the 100% recommended CF, the biofilm with 50% of the recommended CF (50CB) significantly (P < 0.05) reduced soil available Pb2+ by 77%, Cd2+ by 78% and Zn2+ by 62%. In comparison to initial soil, it was 73%, 76%, and 57% lower of Pb2+, Cd2+, and Zn2+, respectively. In addition, 50CB treatment significantly (P < 0.05) reduced the metal penetration into the tuber tissues in comparison with 100 C. Thus, the function of the developed FBB with T. harzianum-B. subtilis can be used as a potential solution to remediate soil polluted with Pb2+ Cd2+ and Zn2+ metal contaminants.","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"25 1","pages":"85"},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83074435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0