Wellcome Open Research最新文献

{"title":"The genome sequence of the Vestal Cuckoo Bee, <i>Bombus vestalis</i> (Geoffroy, 1785).","authors":"Liam M Crowley","doi":"10.12688/wellcomeopenres.24004.1","DOIUrl":"10.12688/wellcomeopenres.24004.1","url":null,"abstract":"<p><p>We present a genome assembly from a haploid male specimen of <i>Bombus vestalis</i> (Vestal Cuckoo Bee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence has a total length of 280.01 megabases. Most of the assembly (91.96%) is scaffolded into 25 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 24.37 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,600 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"218"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chromosome-level genome sequences of the freshwater sponge, <i>Spongilla lacustris</i> (Linnaeus, 1759) and the chlorophyte cobiont <i>Choricystis</i> sp., and the associated microbial metagenome sequences.","authors":"Sally P Leys, Ute Hentschel, Graeme Oatley, Elizabeth Sinclair, Eerik Aunin, Noah Gettle, Camilla Santos, Michael Paulini, Dirk Erpenbeck, Haoyu Niu, Victoria McKenna","doi":"10.12688/wellcomeopenres.23988.1","DOIUrl":"10.12688/wellcomeopenres.23988.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual <i>Spongilla lacustris</i> (freshwater sponge; Porifera; Demospongiae; Spongillida; Spongillidae). The genome sequence is 248.7 megabases in span. Most of the assembly is scaffolded into 23 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 28.04 kilobases in length. A 14.6-megabase genome assembly of the green algal cobiont <i>Choricystis</i> sp. (Chlorophyta; Trebouxiophyceae) was scaffolded into 16 chromosomal pseudomolecules. Additionally, three bacterial metagenome bins were recovered from the same sample. Gene annotation of this assembly at Ensembl identified 30,435 protein coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"222"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening respectful communication with patients and colleagues in neonatal units - developing and evaluating a communication and emotional competence training for nurse managers in Kenya.","authors":"Peris Musitia, Mwanamvua Boga, Dorothy Oluoch, Ane Haaland, Jacinta Nzinga, Mike English, Sassy Molyneux","doi":"10.12688/wellcomeopenres.18006.1","DOIUrl":"10.12688/wellcomeopenres.18006.1","url":null,"abstract":"<p><strong>Introduction: </strong>Effective communication is essential to delivering compassionate, high-quality nursing care. The intensive, stressful and technical environment of a new-born unit (NBU) in a low-resource setting presents communication-related challenges for nurses, with negative implications for nurse well-being, team relationships and patient care. We adapted a pre-existing communication and emotional competence course with NBU nurse managers working in Kenya, explored its' value to participants and developed a theory of change to evaluate its' potential impact.</p><p><strong>Methods: </strong>18 neonatal nurse managers from 14 county referral hospitals helped adapt and participated in a nine-month participatory training process. Training involved guided 'on the job' self-observation and reflection to build self-awareness, and two face-to-face skills-building workshops. The course and potential for future scale up was assessed using written responses from participant nurses (baseline questionnaires, reflective assignments, pre and post workshop questionnaires), workshop observation notes, two group discussions and nine individual in-depth interviews.</p><p><strong>Results: </strong>Participants were extremely positive about the course, with many emphasizing its direct relevance and applicability to their daily work. Increased self-awareness and ability to recognize their own, colleagues' and patients' emotional triggers, together with new knowledge and practical skills, reportedly inspired nurses to change; in turn influencing their ability to provide respectful care, improving their confidence and relationships and giving them a stronger sense of professional identity.</p><p><strong>Conclusion: </strong>Providing respectful care is a major challenge in low-resource, high-pressure clinical settings but there are few strategies to address this problem. The participatory training process examined addresses this challenge and has potential for positive impacts for families, individual workers and teams, including worker well-being. We present an initial theory of change to support future evaluations aimed at exploring if and how positive gains can be sustained and spread within the wider system.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":" ","pages":"223"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11066535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43138817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of Erhard's wall lizard, <i>Podarcis erhardii</i> (Bedriaga, 1882).","authors":"Nathalie Feiner, Tobias Uller, Kinsey M Brock, Panayiotis Pafilis, Joana Meier","doi":"10.12688/wellcomeopenres.24041.1","DOIUrl":"10.12688/wellcomeopenres.24041.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Podarcis erhardii</i> (Erhard's wall lizard; Chordata; Lepidosauria; Squamata; Lacertidae). The assembly contains two haplotypes with total lengths of 1,495.98 megabases and 1,477.75 megabases. Most of haplotype 1 (99.28%) is scaffolded into 20 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 17.14 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"212"},"PeriodicalIF":0.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of Lathbury's Nomad Bee, <i>Nomada lathburiana</i> (Kirby, 1802).","authors":"Liam M Crowley, Steven Falk","doi":"10.12688/wellcomeopenres.24013.1","DOIUrl":"10.12688/wellcomeopenres.24013.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Nomada lathburiana</i> (Lathbury's Nomad Bee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence has a total length of 269.68 megabases. Most of the assembly (90.08%) is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 29.04 kilobases. Gene annotation of this assembly by Ensembl identified 11,607 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"211"},"PeriodicalIF":0.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12177452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the common hop, <i>Humulus lupulus</i> L.","authors":"Maarten J M Christenhusz, Lawrence Percival-Alwyn, Klara Hajdu, John Connell, Ilia J Leitch","doi":"10.12688/wellcomeopenres.24025.1","DOIUrl":"10.12688/wellcomeopenres.24025.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Humulus lupulus</i> plant (Common hop, Hops; Streptophyta; Magnoliopsida; Rosales; Cannabaceae). The genome sequence has a total length of 2,488.10 megabases. Most of the assembly (99.39%) is scaffolded into 10 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial and plastid genome assemblies have lengths of 400.02 kilobases and 153.77 kilobases, respectively. Gene annotation of this assembly by Ensembl identified 32,487 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"210"},"PeriodicalIF":0.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12171724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the common water plantain, <i>Alisma plantago-aquatica</i> L. (Alismataceae).","authors":"Maarten J M Christenhusz, Sahr Mian, Ilia J Leitch","doi":"10.12688/wellcomeopenres.24005.1","DOIUrl":"10.12688/wellcomeopenres.24005.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Alisma plantago-aquatica</i> (common water plantain; Streptophyta; Magnoliopsida; Alismatales; Alismataceae). The genome sequence has a total length of 9,377.97 megabases. Most of the assembly (99.53%) is scaffolded into 7 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 250.4 kilobases and 159.88 kilobases, respectively.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"209"},"PeriodicalIF":0.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic drug monitoring for antimicrobial agents for people living with HIV (TAP).","authors":"Christine Sekaggya-Wiltshire, Eva Agnes Laker Odongpiny, Francis Williams Ojara, Isabella Kyohairwe, Reuben Kiggundu, Hope Mackline, Catriona Waitt, Aida N Kawuma, Allan Kengo, Allan Buzibye, Noela Owarwo, Francis Kakooza, Andrew Kambugu","doi":"10.12688/wellcomeopenres.22707.2","DOIUrl":"10.12688/wellcomeopenres.22707.2","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance (AMR) is a growing health concern, particularly in Africa, and is predicted to become the leading cause of death after cancer by 2050. Factors like overuse or inappropriate use of antibiotics contribute to this crisis. People living with HIV (PLWH) are particularly vulnerable to AMR with potential drug-drug interactions between antiretroviral and antimicrobial agents against common organisms like <i>Mycobacterium tuberculosis.</i> There is limited data on the concentrations of commonly used antimicrobial agents in people living with HIV in resource-limited settings. Therapeutic Drug Monitoring (TDM) offers a promising approach to optimize antibiotic dosing and improve treatment outcomes for those with sub-optimal drug concentrations. TDM has been recommended for PLWH on anti-tuberculosis treatment due to sub-optimal drug concentrations found in a significant proportion of those with TB.</p><p><strong>Objectives: </strong>The main objectives of this study are to determine the concentrations of selected antimicrobial agents in people living with HIV requiring antimicrobial therapy and to assess the utility of therapeutic drug monitoring in achieving therapeutic targets for PLWH receiving rifampicin and isoniazid for the treatment of tuberculosis.</p><p><strong>Methods: </strong>This prospective observational study will enroll adult PLWH receiving amoxicillin, azithromycin, ciprofloxacin, rifampicin, isoniazid, or ceftriaxone. Concentrations of these antibiotics will be measured locally using validated liquid chromatography mass spectrometry methods and high-performance liquid chromatography with ultraviolet detection. TDM with dose adjustment will be performed in a subset of participants on TB treatment. Pharmacokinetic parameters will be estimated using non-linear mixed effects models.</p><p><strong>Results: </strong>This study was reviewed and approved by the research and ethics committee in February 2024. Participant enrolment began in September 2024.</p><p><strong>Conclusions: </strong>We anticipate that the findings from this research will characterize pharmacokinetic and pharmacodynamics relationships to predict treatment response for optimal antimicrobial therapeutic and anti-tuberculosis dosing among people living with HIV (PLWH).</p><p><strong>Clinical registration: </strong>The study is registered with Pan African Clinical Trials Registry, registration number PACTR202409710100607, registration date 07 August 2024, pactr.samrc.ac.za/TrialDisplay.aspx?TrialID=31764.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"694"},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Uncertain moth, <i>Hoplodrina octogenaria</i> (Goeze, 1781).","authors":"Douglas Boyes, Liam M Crowley, Clare Boyes","doi":"10.12688/wellcomeopenres.23983.1","DOIUrl":"10.12688/wellcomeopenres.23983.1","url":null,"abstract":"<p><p>We present a genome assembly from a male specimen of <i>Hoplodrina octogenaria</i> (Uncertain moth; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence has a total length of 476.65 megabases. Most of the assembly (99.98%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled, with a length of 15.93 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"201"},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of a small dung beetle,  <i>Volinus sticticus</i> (Panzer, 1798), formerly known as <i>Aphodius sticticus</i>.","authors":"František Sládeček, Liam M Crowley","doi":"10.12688/wellcomeopenres.23692.1","DOIUrl":"10.12688/wellcomeopenres.23692.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual male specimen of the small dung beetle, <i>Aphodius sticticus</i> (Arthropoda; Insecta; Coleoptera; Scarabaeidae). The genome sequence has a total length of 628.40 megabases. Most of the assembly (98.96%) is scaffolded into 11 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 20.8 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"195"},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}