Wellcome Open Research最新文献

{"title":"The genome sequence of the Queen of Spain Fritillary, <i>Issoria lathonia</i> (Linnaeus, 1758) (Lepidoptera: Nymphalidae).","authors":"Yannick Chittaro, Andreas Sanchez, Camille Cornet, Kay Lucek, Charlotte J Wright, Joana I Meier, Mark L Blaxter","doi":"10.12688/wellcomeopenres.24702.1","DOIUrl":"10.12688/wellcomeopenres.24702.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Issoria lathonia</i> (Queen of Spain Fritillary; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 319.19 megabases and 283.37 megabases. Most of haplotype 1 (99.92%) is scaffolded into 31 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.18 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"427"},"PeriodicalIF":0.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Alpine Zephyr Blue, <i>Kretania trappi</i> (Verity, 1927) (Lepidoptera: Lycaenidae).","authors":"Yannick Chittaro, Kay Lucek, Charlotte J Wright, Joana I Meier, Mark L Blaxter","doi":"10.12688/wellcomeopenres.24703.1","DOIUrl":"10.12688/wellcomeopenres.24703.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Kretania trappi</i> (Alpine Zephyr Blue; Arthropoda; Insecta; Lepidoptera; Lycaenidae). The assembly contains two haplotypes with total lengths of 683.81 megabases and 597.58 megabases. Most of haplotype 1 (98.36%) is scaffolded into 20 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.35 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"432"},"PeriodicalIF":0.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12464535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fediša Modikologo: breaking the intergenerational cycle of violence against women and children. Theoretical framework and protocol for a prospective cohort study.","authors":"Rachel Jewkes, Leane Ramsoomar, Jani Nothling, Samantha Willan, Venice Mbowane, Esnat Chirwa, Shibe Mhlongo, Maureen Phakoe, Desiree Pass, Amanda Zembe, Louis Sibiya, Ishen Seocharan, Charntel Paile, Laura Washington, Nataly Woollett, Bianca Dekel, Nwabisa Jama-Shai, Mercilene Machisa, Pinky Mahlangu, Boitumelo Seepamore, Nicola Christofides, Tracy Glass, Darshini Govindasamy, Stanley Carries, Asiphe Ketelo, Naeemah Abrahams","doi":"10.12688/wellcomeopenres.23513.2","DOIUrl":"10.12688/wellcomeopenres.23513.2","url":null,"abstract":"<p><p>In South Africa, after two decades of national femicide surveillance, we know comparatively little about what places women who experience intimate partner violence (IPV) at risk of intimate partner femicide. Further we have not mapped the multi-generational health, social and economic impact of severe IPV on women subjected to it, and their children, nor the consequences of help-seeking, nor described what helps, STET recovery trajectories. This study aims to deepen understanding of risk factors for femicide and the health, social and economic impacts of severe IPV on women and their families, including understanding risk and resilience to intergenerational cycling of violence. It further aims to describe how statutory and community measures operate to enable recovery and safety. Following pilot research, we developed a prospective questionnaire-based cohort study with three components, and plan for nested qualitative research. The primary cohort will enrol 12,000 women experiencing severe IPV, recruited using non-probabilistic methods (mostly referral from services and community members, and chain-recruitment). Following a baseline interview, participants will complete annual on-line surveys to track key outcomes for five years. The main questionnaire will measure exposure to range of different forms of IPV in the past year, lifetime trauma exposure history, childhood background, health, social and economic circumstances and help-seeking practices. A sub-cohort of the women (a 20% sub-sample), will be followed more intensively over 3 years. Among these, the children aged 6 years and over, of consenting mothers, will also be followed for three years. Deaths in the cohorts will be tracked through the National Population Register through participants' national identity numbers. Mixed-methods verbal autopsies will be conducted with friends or family members of deceased participants. Results will guide femicide prevention nationally, and will build understanding of what is needed to prevent intergenerational cycling of violence and enable recovery of exposed women and children.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"126"},"PeriodicalIF":0.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol to produce a systematic Arenavirus and Hantavirus host-pathogen database: Project ArHa.","authors":"David Simons, Ricardo Rivero, Ana Martinez-Checa Guiote, Harry Luke Mackenzie Gordon, Gregory C Milne, Grant Rickard, David W Redding, Stephanie N Seifert","doi":"10.12688/wellcomeopenres.24037.2","DOIUrl":"10.12688/wellcomeopenres.24037.2","url":null,"abstract":"<p><p>Arenaviruses and Hantaviruses, primarily hosted by rodents and shrews, represent significant public health threats due to their potential for zoonotic spillover into human populations. Despite their global distribution, the full impact of these viruses on human health remains poorly understood, particularly in regions like Africa, where data is sparse. Both virus families continue to emerge, with pathogen evolution and spillover driven by anthropogenic factors such as land use change, climate change, and biodiversity loss. Recent research highlights the complex interactions between ecological dynamics, host species, and environmental factors in shaping the risk of pathogen transmission and spillover. This underscores the need for integrated ecological and genomic approaches to better understand these zoonotic diseases. A comprehensive, spatially, and temporally explicit dataset, incorporating host-pathogen dynamics and human disease data, is crucial for improving risk assessments, enhancing disease surveillance, and guiding public health interventions. Such a dataset (ArHa) would also support predictive modelling efforts aimed at mitigating future spillover events. This paper proposes the development of this unified database for small-mammal hosts of Arenaviruses and Hantaviruses, identifying gaps in current research and promoting a more comprehensive understanding of pathogen prevalence, spillover risk, and viral evolution.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"227"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"States, law, and the regulation of controversial health-related claims: consolidating a research agenda between disciplines and contexts.","authors":"Emilie Cloatre, Martyn Pickersgill, Caesar A Atuire, Mairead Enright, Phoebe Friesen, Patricia Kingori, Tidiane Ndoye, Nayeli Urquiza-Haas","doi":"10.12688/wellcomeopenres.24597.1","DOIUrl":"10.12688/wellcomeopenres.24597.1","url":null,"abstract":"<p><p>Stories of unproven, disproven, or misleading health-related claims, and their impact on individual and public health, are commonplace around the world. Disquiet about such claims is ubiquitous and growing within public, clinical, scientific, and policy discourse, with law commonly presented as having an important role to play in addressing concerns. Action, though, requires regulators to account for competing considerations, including fundamental freedoms, cultural diversity, and the potential for law to exacerbate inequalities. The latter is particularly significant when assessing the veracity of marginalised beliefs. In practice, legal decision-makers walk a fine line between everyday tolerance and occasional intervention. Yet, legal research pertinent to these issues is surprisingly limited. Here, we argue that new knowledge, methods, and collaborations are needed to better understand how regulatory interventions relevant to contested claims are constituted; how they operate in practice; and how they relate to different political and social processes - including acts of public resistance (like campaigns and protests). Only once we are collectively equipped with such critical knowledge of the current nature and possibilities of regulatory relations will it be possible to collectively design more imaginative and inclusive legal responses.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"414"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Piedmont Ringlet, <i>Erebia meolans</i> von Prunner, 1798 (Lepidoptera: Nymphalidae).","authors":"Marta Vila, Kay Lucek, Charlotte J Wright, Joana I Meier, Mark L Blaxter","doi":"10.12688/wellcomeopenres.24665.1","DOIUrl":"10.12688/wellcomeopenres.24665.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Erebia meolans</i> (Piedmont Ringlet; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 512.88 megabases and 439.35 megabases. Most of haplotype 1 (95.0%) is scaffolded into 15 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.24 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"408"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Map, <i>Araschnia levana</i> (Linnaeus, 1758) (Lepidoptera: Nymphalidae).","authors":"Mathieu Joron, Kay Lucek, Charlotte J Wright, Joana I Meier, Mark L Blaxter","doi":"10.12688/wellcomeopenres.24633.1","DOIUrl":"10.12688/wellcomeopenres.24633.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Araschnia levana</i> (Map; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 362.54 megabases and 332.31 megabases. Most of haplotype 1 (99.09%) is scaffolded into 32 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.96 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"398"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Dusky Meadow Brown, <i>Cercyonis lycaon</i> (Lepidoptera: Nymphalidae).","authors":"Yannick Chittaro, Eric Toro-Delgado, Kay Lucek, Charlotte J Wright, Joana I Meier, Mark L Blaxter","doi":"10.12688/wellcomeopenres.24656.1","DOIUrl":"10.12688/wellcomeopenres.24656.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Cercyonis lycaon</i> (Dusky Meadow Brown; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 601.00 megabases and 548.79 megabases. Most of haplotype 1 (94.99%) is scaffolded into 30 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.2 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"395"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Silver-washed Fritillary, <i>Argynnis paphia</i> (Linnaeus, 1758) (Lepidoptera: Nymphalidae).","authors":"Yannick Chittaro, Andreas Sanchez, Kay Lucek, Charlotte J Wright, Joana I Meier, Mark L Blaxter","doi":"10.12688/wellcomeopenres.24635.1","DOIUrl":"10.12688/wellcomeopenres.24635.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Argynnis paphia</i> (Silver-washed Fritillary; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 520.88 megabases and 444.74 megabases. Most of haplotype 1 (97.96%) is scaffolded into 30 chromosomal pseudomolecules, including the W, Z ₁, and Z ₂ sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.22 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"399"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Genetic evolution of <i>Burkholderia pseudomallei</i> during treatment leading to antibiotic resistance and disease relapse.","authors":"Terry John Evans, Chantisa Keeratipusana, Anousone Douangnouvong, Vilayouth Phimolsarnnousith, Davanh Sengdatka, Ko Chang, Koukeo Phommasone, Claire Chewapreecha, Elizabeth A Ashley, Elizabeth M Batty","doi":"10.12688/wellcomeopenres.24138.2","DOIUrl":"10.12688/wellcomeopenres.24138.2","url":null,"abstract":"<p><strong>Background: </strong>Melioidosis is a significant yet neglected cause of sepsis in tropical regions, particularly in southeast Asia, with poor clinical outcomes. It is a growing threat with an expanding global footprint. The causative organism, <i>Burkholderia pseudomallei</i>, is intrinsically resistant to most first-line empiric antibiotic regimens, but acquired resistance to recommended antibiotics for this infection is uncommon. Nonetheless, the genetic determinants of resistance in this species remain poorly elucidated.</p><p><strong>Case presentation: </strong>A 60-year-old farmer presented in septic shock to a hospital in Laos, and <i>B. pseudomallei</i> was grown from blood cultures. Following initial antibiotic treatment with meropenem and co-trimoxazole, his infection relapsed. Several subsequent <i>B. pseudomallei</i> isolates from the patient were resistant to multiple antibiotics, and whole genome sequencing demonstrated that this phenotype was associated with a novel 54-kb genomic deletion. This deletion, on chromosome 1, includes the 5' end of <i>amrR</i> - which encodes a regulator of an efflux pump known to be important in conferring meropenem resistance - as well as 46 other genes, some of which have not been characterised. Treatment was targeted to the new antibiogram, requiring a further prolonged intravenous course and second-line oral eradication therapy. The patient made a full recovery.</p><p><strong>Conclusions: </strong>Mutations in <i>Burkholderia pseudomallei</i> lead to increased virulence and drug resistance. Repeat microbiological sampling of patients who do not make clinical improvement as anticipated is essential, with repeat full antimicrobial susceptibility testing on subsequent isolates. Characterisation of drug-resistant mutants is required to understand mechanisms of resistance and to predict phenotypes from whole genome sequencing.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"281"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}