Wellcome Open Research最新文献

{"title":"Mechanisms through which exercise reduces symptom severity and/or functional impairment in posttraumatic stress disorder (PTSD): Protocol for a living systematic review of human and non-human studies.","authors":"Simonne Wright, Toshi A Furukawa, Malcolm Macleod, Ouma Simple, Olufisayo Elugbadebo, Virginia Chiocchia, Claire Friedrich, Edoardo G Ostinelli, Jennifer Potts, Fiona J Ramage, Spyridon Siafis, Claire Stainsfield, Francesca Tinsdeall, James Thomas, Andrea Cipriani, Georgia Salanti, Soraya Seedat","doi":"10.12688/wellcomeopenres.19903.3","DOIUrl":"10.12688/wellcomeopenres.19903.3","url":null,"abstract":"<p><strong>Background: </strong>Exercise can play an important role in reducing symptom severity and improving functional impairment in patients with posttraumatic stress disorder (PTSD). However, the precise mechanisms underpinning the effect of exercise in PTSD management are not fully understood. This living systematic review aims to synthesize and triangulate the evidence from non-human and human studies to gain insight into the biopsychosocial mechanisms through which exercise reduces symptom severity and functional impairment.</p><p><strong>Methods: </strong>Independent searches will be conducted in electronic databases to identify eligible studies. Two reviewers will independently conduct the study selection, data extraction, and risk of bias assessment. We will extract outcome data and variables that can act as effect modifiers or as mediators of the effect of exercise. For the non-human studies, outcome data will include the non-human equivalents of PTSD symptom clusters. For human studies, the primary outcome will be PTSD symptom severity. The secondary outcomes will be avoidance symptom severity, reexperiencing symptom severity, hyperarousal symptom severity, negative cognitions and mood severity, functional impairment, loss of PTSD diagnosis, and dropout rates.To explain the biopsychosocial mechanisms through which exercise affects the outcome of interest, we will extract effects that relate to the impact of exercise on potential mediating variables and the effect of the later outcomes. Comparison of within-study direct and indirect effects obtained from mediation analysis, when reported, will provide insight into the importance of the examined mediator.If appropriate, we will synthesize study results using meta-analyses. We will examine potential effect modifiers of the total exercise effect to understand better the impact of exercise on PTSD symptoms and function impairment (when possible). The evidence about the potential mediators of the association between exercise and PTSD-related outcomes will be considered in a consensus meeting when sufficient evidence is available.</p><p><strong>Protocol registration: </strong>PROSPERO-ID: 453615.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"8 ","pages":"494"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of a cluster fly, <i>Pollenia pediculata</i> Macquart, 1834.","authors":"Steven Falk, Liam M Crowley, Olga Sivell","doi":"10.12688/wellcomeopenres.23961.1","DOIUrl":"10.12688/wellcomeopenres.23961.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Pollenia pediculata</i> (cluster fly; Arthropoda; Insecta; Diptera; Polleniidae). The assembly contains two haplotypes with total lengths of 1,156.86 megabases and 1,222.48 megabases. Most of haplotype 1 (99.79%) is scaffolded into 6 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.82 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"190"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZC3HC1 has functions distinct from TPR and is dispensable for TPR localisation to the nuclear basket.","authors":"Bethany M Bartlett, Juan Carlos Acosta, Wendy A Bickmore","doi":"10.12688/wellcomeopenres.23711.1","DOIUrl":"10.12688/wellcomeopenres.23711.1","url":null,"abstract":"<p><strong>Background: </strong>The nuclear basket is a 'fishtrap'-like structure on the nucleoplasmic face of the nuclear pore complex which has been implicated in diverse functions including RNA export, heterochromatin organisation, and mitosis. Recently, a novel component of the nuclear basket, ZC3HC1, has been described. The localisation of ZC3HC1 to nuclear pores has been reported to occur reciprocally with TPR, a major structural component of the nuclear basket.</p><p><strong>Methods: </strong>Using siRNA-mediated knock down, immunofluorescence and RNA sequencing we compare the consequences of depleting two proteins of the nuclear pore basket - TPR and ZC3HC1.</p><p><strong>Results: </strong>We show that in human fibroblasts, although ZC3HC1 localisation to nuclear pores is TPR-dependent, TPR localises to pores regardless of the presence of ZC3HC1. We demonstrate that knockdown of TPR and ZC3HC1 produce distinct transcriptional profiles.</p><p><strong>Conclusions: </strong>Our results suggest that there is little overlap in function between these two nuclear basket proteins in human diploid fibroblasts.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"188"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the short-beaked common dolphin, <i>Delphinus delphis</i> Linnaeus, 1758.","authors":"Nicholas J Davison, Phillip A Morin","doi":"10.12688/wellcomeopenres.23918.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23918.1","url":null,"abstract":"<p><p>We present a genome assembly from a male specimen of <i>Delphinus delphis</i> (short-beaked common dolphin; Chordata; Mammalia; Artiodactyla; Delphinidae). The genome sequence has a total length of 2,663.52 megabases. Most of the assembly (88.76%) is scaffolded into 23 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled, with a length of 16.39 kilobases. Gene annotation of this assembly at Ensembl identified 17,797 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"178"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the harbour porpoise, <i>Phocoena phocoena</i> (Linnaeus, 1758).","authors":"Nicholas J Davison, Phillip A Morin","doi":"10.12688/wellcomeopenres.24011.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.24011.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Phocoena phocoena</i> (harbour porpoise; Chordata; Mammalia; Artiodactyla; Phocoenidae). The genome sequence has a total length of 2,512.71 megabases. Most of the assembly (94.41%) is scaffolded into 22 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 16.38 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"181"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of a snail-killing fly, <i>Dichetophora obliterata</i> (Fabricius, 1805).","authors":"Liam M Crowley, Olga Sivell, Ryan Mitchell, Duncan Sivell","doi":"10.12688/wellcomeopenres.23993.1","DOIUrl":"10.12688/wellcomeopenres.23993.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Dichetophora obliterata</i> (snail-killing fly; Arthropoda; Insecta; Diptera; Sciomyzidae). The genome sequence has a total length of 1,312.79 megabases. Most of the assembly (99.78%) is scaffolded into 6 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 21.36 kilobases. Gene annotation of this assembly on Ensembl identified 15,139 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"176"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of long-finned pilot whale, <i>Globicephala melas</i> (Traill, 1809).","authors":"Nicholas J Davison, Phillip A Morin","doi":"10.12688/wellcomeopenres.23919.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23919.1","url":null,"abstract":"<p><p>We present a genome assembly from a male specimen of <i>Globicephala melas</i> (long-finned pilot whale; Chordata; Mammalia; Artiodactyla; Delphinidae). The genome sequence has a total length of 2,651.28 megabases. Most of the assembly (89.15%) is scaffolded into 23 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled, with a length of 16.39 kilobases. Gene annotation of this assembly on Ensembl identified 17,911 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"180"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12009481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the European ground squirrel, <i>Spermophilus citellus</i> (Linnaeus, 1766).","authors":"Dimitra-Lida Rammou, Dionisios Youlatos, Alexandros Triantafyllidis","doi":"10.12688/wellcomeopenres.23974.1","DOIUrl":"10.12688/wellcomeopenres.23974.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Spermophilus citellus</i> (European ground squirrel; Chordata; Mammalia; Rodentia; Sciuridae). The genome sequence has a total length of 3,090.03 megabases. Most of the assembly (95.47%) is scaffolded into 20 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 16.45 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"184"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of <i>Glossophaga mutica</i> (Chiroptera, Phyllostomidae, Glossophaginae; Merriam, 1898).","authors":"Nancy B Simmons, Melissa R Ingala, Brian P O'Toole, Linelle Abueg, Kirsty McCaffrey, Bonhwang Koo, Giulio Formenti, Erich D Jarvis, Myrtani Pieri, Meike Mai, Larry N Singh, Philge Philip, Laramie L Lindsey, Ning Zhang, Jonathan L Gray, Emma C Teeling, Sonja C Vernes","doi":"10.12688/wellcomeopenres.23611.1","DOIUrl":"10.12688/wellcomeopenres.23611.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual female <i>Glossophaga mutica</i> (Chordata; Mammalia; Chiroptera; Phyllostomidae). The genome sequence is 2.13 in span. The majority of the assembly is scaffolded into 32 chromosomal pseudomolecules, with the X sex chromosome assembled.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"174"},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of coxsackievirus A24 variant during an acute hemorrhagic conjunctivitis outbreak in coastal Kenya, 2024.","authors":"Arnold W Lambisia, John Mwita Morobe, Edidah Moraa, Salim Mwarumba, Fredrick K N Korir, Raila Seif Athman, Rebecca Kiptui, Micheal Mbee, Nelly Mugo, Patrick Amoth, Penny Muange, Charlotte J Houldcroft, Edwine Barasa, Joseph Mwangangi, George Githinji, Edward C Holmes, Lynette Isabella Ochola-Oyier, Charles N Agoti","doi":"10.12688/wellcomeopenres.23522.2","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23522.2","url":null,"abstract":"<p><strong>Background: </strong>In early 2024, a surge in acute hemorrhagic conjunctivitis (AHC), also referred as \"red eye\" disease, was observed in coastal Kenya, prompting the Ministry of Health to issue an outbreak alert. Herein, we investigated the etiology of this outbreak.</p><p><strong>Methods: </strong>Ocular swabs were obtained from 13 individuals presenting with AHC at a Mombasa clinic in early February 2024. Ten of these were analyzed using bacterial cultures, and all 13 using a pan-adenovirus quantitative PCR (qPCR) and metagenomic sequencing. Potential viral etiology was confirmed by a specific qPCR, amplicon sequencing and phylogenetic analysis.</p><p><strong>Results: </strong>Bacterial cultures yielded no growth except in three samples where non-pathogenic bacteria were detected. All 13 samples were adenovirus qPCR negative. Metagenomic sequencing detected coxsackievirus A24 variant (CA24v) in three of the 13 samples. CV-A24v detections were confirmed by both CV-A24v specific qPCR and amplicon sequencing of an approximately 450 nucleotide long VP4/2 junction genomic region. Phylogenetic analysis of the VP4/2 sequences showed that they were closely related to CV-A24v genotype IV.</p><p><strong>Conclusion: </strong>The AHC epidemic in coastal Kenya in early 2024 was likely caused by CA24v. Metagenomic sequencing is a powerful tool for identifying potential causative agents of new disease outbreaks.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"28"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}