Voprosy virusologii最新文献

{"title":"[Influenza virus infection affects the efficacy of the treatment of dyslipidemia in mice with a polyisoprenoid-based drug].","authors":"E A Makiev, T N Kozhevnikova, A V Pronin, A N Narovlyansky, A V Sanin, I V Ganshina, M M Shmarov","doi":"10.36233/0507-4088-343","DOIUrl":"10.36233/0507-4088-343","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate how influenza infection affects the hypolipidemic effect of a novel combined drug - Prenophytol based on polyisoprenoids (sodium polyprenyl phosphate and beta-sitosterol) in mice with experimental dyslipidemia.</p><p><strong>Materials and methods: </strong>In BALB/c mice with experimentally induced dyslipidemia, the impact of Prenophytol on lipid metabolism parameters was assessed. Currently the drug is at the stage of clinical trials (Roszdravnadzor's permission to conduct clinical trials No. 362 dated 08/14/2025). A subset of animals was infected with influenza A/California/07/2009 (H1N1) virus.</p><p><strong>Results: </strong>Administration of the drug to dyslipidemia mice resulted in a significant reduction in total cholesterol, triglycerides, and LDL levels, along with a significant increase in HDL. However, in the presence of influenza infection, the hypolipidemic effect of Prenophytol was markedly attenuated: only cholesterol levels decreased significantly, whereas changes in triglycerides, LDL, and HDL did not reach statistical significance.</p><p><strong>Conclusions: </strong>Influenza infection diminishes the therapeutic efficacy of the Prenophytol in the treatment of dyslipidemia. These findings indicate that concomitant viral infections must be taken into account when designing dyslipidemia treatment regimens and conducting clinical trials of this drug.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 2","pages":"139-149"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148035711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular characterization of a rare rotavirus (<i>Sedoreoviridae</i>: <i>Rotavirus</i>) strain genotype G2P[9] isolated from a child with acute gastroenteritis].","authors":"E I Velikzhanina, T A Sashina, A Y Kashnikov, N V Epifanova, N A Novikova","doi":"10.36233/0507-4088-369","DOIUrl":"10.36233/0507-4088-369","url":null,"abstract":"<p><strong>Introduction: </strong>Rotaviruses exhibit a high degree of variability, which is ensured by the process of reassortment and antigenic drift. This leads to significant genetic diversity, including the emergence of strains with rare and unusual genotypes. This study, for the first time, provides a molecular genetic characterization of a rare RVA strain with the genotype G2P[9] based on all genome segments.</p><p><strong>Aim of the study: </strong>To conduct a molecular genetic characterization of a rare strain of RVA genotype G2P[9] based on all genome segments, as well as to compare the strain with previously characterized representatives of subgroup BA222 from Nizhny Novgorod.</p><p><strong>Materials and methods: </strong>Rotavirus-positive stool samples from children were analyzed using PCR-genotyping and PAGE. For the isolate under study, cDNA fragments of each of the 11 genes (<i>VP1-VP4</i>, <i>VP6</i>, <i>VP7</i>, <i>NSP1-NSP5</i>) were sequenced. Nucleotide and amino acid sequence analysis and phylogenetic tree construction were performed using MEGA X.</p><p><strong>Results: </strong>In the period 2022-2023, a single strain of the unique genotype G2P[9] was identified in Nizhny Novgorod, characterized by a \"broad\" RNA electrophoretype (G2-P[9]-I2-R2-C2-M2-A3-N2-T3-E3-H3). The study showed that the origin of this strain is associated with reassortment processes between Nizhny Novgorod Wa- and BA222-like RVA, as well as isolates of the G3P[9] genotype from China. The studied strain, along with rotaviruses from China, differed from the Nizhny Novgorod BA222-like strains by the presence of 43 nucleotide substitutions in nine genes. These differences resulted in ten amino acid substitutions, eight of which were radical. All radical substitutions were located in the functionally active regions of the VP1, VP3, and VP4 proteins, which play a key role in the replicative cycle of rotaviruses.</p><p><strong>Discussion: </strong>We characterized a strain with the rare G2P[9] genotype based on its complete genotype for the first time in Russia and globally. These results expand our understanding of the diversity of reassortant rotaviruses and complement our knowledge of the genotypic structure of the rotavirus population in Nizhny Novgorod.</p><p><strong>Conclusion: </strong>The wide genetic diversity of rotaviruses, maintained by reassortment and antigenic drift, may facilitate rotaviruses' ability to overcome immunological pressure. Therefore, continuous molecular monitoring of circulating rotavirus variants is necessary to monitor the emergence of new variants and assess changes in rotavirus virulence following reassortment processes.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 2","pages":"182-194"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148035754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Test systems for the laboratory detection and differentiation of Powassan virus from tick-borne encephalitis virus (<i>Flaviviridae</i>: <i>Orthoflavivirus</i>) using RT-PCR and RT-qPCR].","authors":"M P Fedina, R I Ivannikov, G G Karganova, A G Litov","doi":"10.36233/0507-4088-364","DOIUrl":"10.36233/0507-4088-364","url":null,"abstract":"<p><strong>Introduction: </strong>Powassan virus (POWV) is a tick-borne orthoflavivirus capable of causing neurological diseases. In the Russian Far East, the area affected by POWV overlaps with that of another neurotropic orthoflavivirus, tick-borne encephalitis virus (TBEV). Currently, there are no differentiated test systems for specific detection of POWV in the presence of TBEV. The aim of this work is to create differentiating test systems for the detection of POWV in a mixture with TBEV and other orthoflaviviruses.</p><p><strong>Materials and methods: </strong>We constructed a genome alignment of eight POWV and five TBEV strains to select differentiating primers using MEGA X. To assess the specificity of the assay, we used POWV, several TBEV strains, and other orthoflaviviruses. PCR and qPCR were performed with the selected oligonucleotides, and the specificity of amplification was verified by electrophoresis in agarose gel and Sanger sequencing. cDNA was obtained from virus-containing material by isolating RNA and performing a reverse transcription reaction.</p><p><strong>Results: </strong>The selected oligonucleotides for the qPCR-based differentiation system ensured specific detection of POWV without detecting signals from other orthoflaviviruses. The assay's limit of detection was 10² copies/sample. Analysis of mixed samples containing POWV and TBEV demonstrated no distortion of the measurement results. Furthermore, differentiating oligonucleotides were selected and tested to amplify extended regions of the POWV and TBEV genomes for subsequent sequencing and phylogenetic analysis.</p><p><strong>Conclusion: </strong>A laboratory technique based on PCR has been developed that allows to detect the POWV in samples containing other orthoflaviviruses.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 2","pages":"162-174"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148035767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Serological tests based on viral glycoproteins for detecting neutralizing antibodies to measles, mumps and rubella viruses].","authors":"F G Nagieva, E P Barkova, O S Kharchenko, A V Sidorov, N N Vlasova, O A Trubacheva, Y N Tarakanova, V V Semerikov, A K Tagirova, D A Yakovleva, E A Pashkov, E S Osipova, A M Polisadova, O A Svitich, V V Zverev","doi":"10.36233/0507-4088-354","DOIUrl":"10.36233/0507-4088-354","url":null,"abstract":"<p><strong>Introduction: </strong>Measles, mumps and rubella viruses are highly contagious viruses that can cause serious clinical outcomes, complications and death. Neutralizing antibodies against measles, mumps and rubella viruses are a good indicator of clinical protection against these infections, but they are difficult to measure.</p><p><strong>The aim: </strong>To develop a serological glycoprotein-based passive hemagglutination reaction (gpPHAR) tests for the detection of neutralizing antibodies to measles, mumps and rubella viruses.</p><p><strong>Materials and methods: </strong>Human and animal cell cultures from the collection of the I.I. Mechnikov Research Institute of Vaccines and Sera were used; vaccine strains of measles, mumps and rubella viruses; monoclonal antibodies to measles and rubella virus glycoproteins, human and animal immune sera; formalized sheep erythrocytes sensitized with viral glycoproteins; virus neuralization test (VNT), gpPHAR and gpELISA.</p><p><strong>Results: </strong>Viral glycoproteins of measles, mumps, and rubella were extracted from infected cell cultures. Comparative titrations of human and animal immune sera to measles, mumps, and rubella viruses in VNT, gpPHAR, and gpELISA have been performed. Unambiguous titers of neutralizing antibodies have been established. Titration of homologous and heterologous immune sera to three viruses was performed in the gpPHAR. The absence of cross-immunoreactivity to other viral agents has been established. The titers of the immune sera of patients with measles, mumps, and rubella infections were determined in the gpPHAR and gpELISA assays. Identical titers of neutralizing antibodies were found in both serological tests.</p><p><strong>Conclusion: </strong>The gpPHAR serological tests has been developed to evaluate neutralizing virus-specific antibodies to measles, mumps and rubella in patients.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 2","pages":"127-138"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148035746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Rapid differentiation of genotype I and new recombinant variant of African swine fever virus (<i>Asfarviridae</i>: <i>Asfivirus</i>) using real-time PCR].","authors":"R S Chernyshev, E O Morozova, A S Sadchikova, D S Moiseenko, A S Igolkin","doi":"10.36233/0507-4088-361","DOIUrl":"10.36233/0507-4088-361","url":null,"abstract":"<p><strong>Introduction: </strong>The identification of I/II genotype recombinant African swine fever virus (ASFV) in China (2021) and its subsequent introduction to Primorsky region of Russia (2023) is a new challenge in control against ASF. The potential recombinant virus circulation may negate efforts to develop vaccines against ASF, vaccines that are based on genotype II strains) do not provide protection against recombinant virus. The aim of the study was to develop qPCR for differentiation of genotype I and new recombinant ASFV variant in clinical samples from infected domestic pigs and wild boars.</p><p><strong>Materials and methods: </strong>Samples of viral culture suspensions and biological material from pigs, ASF- free and infected with the recombinant ASFV strain Primorsky 2023/DP-4560 or genotype II strains were used. Positive control sample and standard plasmid were obtained using complex molecular cloning techniques to construct circular and linearized forms of plasmid pJET1.2_IREC.</p><p><strong>Results: </strong>Primers and a TaqMan probe (modified with LNA, locked linear nucleic acid) were designed to the fragment of <i>MGF 110-1L</i> gene (93 bp). The specificity of RT-PCR was enhanced by increasing the annealing temperature to 65°C. Developed method had precision (repeatability (CV = 0.7-1.3%) and reproducibility (CV = 2.76-5.69%), 100% diagnostic specificity, 96% clinical sensitivity, and high linear dynamic range (R²= 99.62) and efficiency (E = 95%). The limit of detection was 7 copies/µL (95% CI: 4-10 copies/µL) or 70 copies/per reaction.</p><p><strong>Conclusion: </strong>The monoplex RT-PCR has been developed to study the extent of circulation in Russia of atypical variants originating from East Asia.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 2","pages":"150-161"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148035757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of candidate vaccines against Tick-borne encephalitis based on mRNA, simian adenovirus serotype 25 and a chimeric replication-competent yellow fever vaccine strain].","authors":"A E Siniavin, V A Gushchin, N A Kuznetsova, L I Russu, E V Marchuk, D A Kleymenov, M A Nikiforova, O V Zubkova, O D Popova, I V Vavilova, T A Ozharovskaia, E P Mazunina, E N Bykonia, E V Shidlovskaya, E V Usachev, O V Usacheva, A A Pochtovyi, V I Zlobin, D Y Logunov, A L Gintsburg","doi":"10.36233/0507-4088-353","DOIUrl":"10.36233/0507-4088-353","url":null,"abstract":"<p><strong>Introduction: </strong>The spread of tick-borne encephalitis virus (<i>Orthoflavivirus encephalitidis</i>) continues. Existing vaccines have several limitations. Research new vaccine platforms will enable the development of more effective strategies of disease prevention.</p><p><strong>Materials and methods: </strong>The constructs for producing vaccine preparations were obtained using the methods described earlier. Study of the effectiveness of candidate vaccines based on chimeric yellow fever virus, adenovirus serotype 25 and mRNA encoding a fragment of the tick-borne encephalitis viral polyprotein corresponding to the PrM and E regions, immunogenicity and protective efficacy was performed using BALB/c mice. The neutralizing activity assessment of animal sera and mice challenge were performed using the tick-borne encephalitis virus strain Sofjin-Chumakov.</p><p><strong>Results: </strong>It was established that immunization of animals with mRNA encoding the PrM and E regions of the viral polyprotein of tick-borne encephalitis virus resulted in the induction of a higher level of neutralizing antibodies compared to the other vaccine platforms studied in the immunization schedule used. In addition, this platform provided 100% protection of animals from the lethal infection caused by the tick-borne encephalitis virus.</p><p><strong>Conclusion: </strong>The study demonstrated that delivery of the nucleotide sequence encoding the PrM and E regions of the tick-borne encephalitis virus polyprotein within mRNA elicits a pronounced humoral immune response during animal immunization. These results demonstrate the fundamental feasibility of effectively expressing the same tick-borne encephalitis virus antigen in various vaccine platforms, each with its own advantages and limitations.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 2","pages":"109-126"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148035758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Georgy Artemyevich Galegov (1931-2026).","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 1","pages":"83-84"},"PeriodicalIF":0.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of the interferon inducer tilorone and its combination with antibacterial drugs in a murine model of secondary bacterial pneumonia caused by <i>S. aureus</i> after influenza infection.","authors":"I N Falynskova, A A Poromov, N A Mikhailova, N P Kartashova, E A Glubokova, A V Ivanina, N V Gudova, I A Leneva","doi":"10.36233/0507-4088-363","DOIUrl":"10.36233/0507-4088-363","url":null,"abstract":"<p><strong>Introduction: </strong>Secondary bacterial pneumonias are complications responsible for most fatalities following influenza infection, and antiviral drugs are used to prevent their development. The aim of the study is to evaluate the efficacy of tilorone and its combination with antibacterial agents in a murine model of secondary bacterial pneumonia caused by <i>Staphylococcus aureus</i> after influenza infection.</p><p><strong>Materials and methods: </strong>BALB/c mice were infected with influenza virus A/California/04/2009 (pdm H1N1 2009) virus, followed by <i>S.</i> <i>aureus</i> infection four days later. Treatments included the interferon inducer tilorone, comparator oseltamivir, antibacterial agents cefuroxime and amoxicillin, or combinations of antivirals with antibiotics. Efficacy was assessed by increased survival, reduced weight loss, and inhibition of pathogen proliferation in the lungs.</p><p><strong>Results: </strong><i> </i>The efficacy of tilorone, as indicated by increased survival, reduced weight loss, and decreased pathogen load in the lungs, in a mouse model of secondary bacterial pneumonia following influenza infection, increased with reducing viral dose, and intraperitoneal administration of the drug was more effective than oral administration. The combination of tilorone with the antibiotic cefuroxime, to which <i>S. aureus</i> was sensitive, was more effective than either agent alone and completely protected animals from death and from viral and bacterial proliferation in the lungs. In contrast, the combination with amoxicillin, to which <i>S. aureus</i> was resistant, had no effect on disease outcome.</p><p><strong>Conclusion: </strong>The efficacy of tilorone in this model depended on viral dose and drug administration route. Combining tilorone with cefuroxime was more effective than monotherapy.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 1","pages":"73-82"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Results of a blood serum examination of residents of Moscow and the Moscow region after the end of the West Nile fever outbreak in 2021.","authors":"A M Butenko, I S Kholodilov, I V Dailak, M S Baranets, G G Karganova, M V Bazarova, I V Ivanova, N A Barsukova, P O Bogomolov","doi":"10.36233/0507-4088-327","DOIUrl":"10.36233/0507-4088-327","url":null,"abstract":"<p><p>The aim of this study was to determine the level of humoral immunity to the West Nile virus (WNV) in the Moscow region population after the end of the outbreak in October 2021, as well as to confirm the specificity of antibodies to WNV by comparatively testing patient sera for antibodies to the antigenically related endemic tick-borne encephalitis orthoflavivirus (TBEV) using ELISA-IgM, ELISA-IgG, and 50% plaque reduction neutralization test.</p><p><strong>Materials and methods: </strong>We analyzed 1,594 sera from outpatients-residents of Moscow and the surrounding region-who underwent outpatient examination in the winter of 2021 at Infectious Diseases Clinical Hospital No. 1 in Moscow (IKB No. 1) and medical institutions of the Moscow Regional Research Institute named after M.F. Vladimirsky Regional Research Institute (MONIKI) conducted a study without any connection to WNV infection in the summer-autumn of 2021.</p><p><strong>Results: </strong>All samples were negative in ELISA-IgM test with WNV and TBEV antigens. In an ELISA-IgG test with the WNV antigen, antibodies were detected in 64 samples (4.0%). All samples were tested for IgG antibodies to TBEV in an ELISA and neutralizing antibodies to WNV and TBEV in a 50% plaque reduction neutralization test. Specific antibodies to WNV were detected in 44 samples (68.8%), to TBEV in eleven (17.2%), and group-specific antibodies in nine (14.0%). According to the total data from the test of 1,594 sera from residents of the Moscow region, specific antibodies to WNV were detected in 2.8% of cases, to TBEV in 0.7%, and group-specific antibodies in 0.6%. Ten of the 11 individuals with specific IgG antibodies to TBEV were undergoing outpatient examination at Moscow's Infectious Diseases Clinical Hospital No. 1 for a history of tick-borne encephalitis or for post-vaccination immunity testing following vaccination against this infection. The detection rate of specific antibodies to WNV in similar studies conducted in the same region in 2013 was 0.2%, while in 2021 it was 2.8%. The difference between these rates is statistically significant (<i>p </i>< 0.01).</p><p><strong>Conclusion: </strong>Based on these data, it can be concluded that sporadic undiagnosed cases of WNV infection occurred in Moscow and the surrounding region between 2013 and 2021.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 1","pages":"32-41"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Yezo virus (<i>Nairoviridae, Orthonairovirus, Orthonairovirus yezoense</i>) circulation in <i>Ixodes persulcatus</i> ticks in the Amur Region, Russia.","authors":"M Y Kartashov, V Y Kurushina, A S Zheleznova, K A Svirin, E I Materikina, V A Ternovoi","doi":"10.36233/0507-4088-358","DOIUrl":"10.36233/0507-4088-358","url":null,"abstract":"<p><strong>Introduction: </strong>The unfavorable epidemiological situation with viral tick-borne infections can be characterized not only by an increase in the incidence of «classical», well-known diseases, but also by the discovery of new pathogens, the role of which remains poorly understood. <i>Yezo virus</i> (YEZV) belongs to the group of recently discovered tick-borne nairoviruses. YEZV circulation is characteristic of Japan, China, and a number of regions in Russia (Primorsky, Khabarovsk, and Zabaykalsky Krais; Tomsk Oblast). The aim of the study was to screen and characterize the molecular genetics of YEZV isolated from ticks in the Amur region.</p><p><strong>Materials and methods: </strong>In the study, 704 individual tick samples (463 <i>I. persulcatus</i> ticks and 241 <i>H. concinna</i> ticks) collected from vegetation in 15 locations in 5 districts of the Amur Region were analyzed for the presence of YEZV RNA. Full-length genomic sequencing of the identified YEZV genetic variant was performed by preliminary enrichment of YEZV cDNA using multiplex PCR with a primer panel developed by us.</p><p><strong>Results: </strong>The YEZV infection rate in the studied sample of <i>I. persulcatus</i> ticks was 0.2% (1/463; 95% CI: 0.1-1.2).</p><p><strong>Conclusion: </strong>The results of this study expand our understanding of the spread of YEZV in Russia. Up-to-date information on the circulation and genetic diversity of nairoviruses that pose a potential threat to humans and animals contributes to the regulation and adjustment of preventive and anti-epidemic measures, as well as the implementation of adequate region-oriented laboratory diagnostics of IPK in the Far East, and especially in areas bordering China.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 1","pages":"53-61"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}