Results of a blood serum examination of residents of Moscow and the Moscow region after the end of the West Nile fever outbreak in 2021.

Q3 Medicine

Voprosy virusologii Pub Date : 2026-02-28 DOI:10.36233/0507-4088-327

A M Butenko, I S Kholodilov, I V Dailak, M S Baranets, G G Karganova, M V Bazarova, I V Ivanova, N A Barsukova, P O Bogomolov

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引用次数: 0

Abstract

The aim of this study was to determine the level of humoral immunity to the West Nile virus (WNV) in the Moscow region population after the end of the outbreak in October 2021, as well as to confirm the specificity of antibodies to WNV by comparatively testing patient sera for antibodies to the antigenically related endemic tick-borne encephalitis orthoflavivirus (TBEV) using ELISA-IgM, ELISA-IgG, and 50% plaque reduction neutralization test.

Materials and methods: We analyzed 1,594 sera from outpatients-residents of Moscow and the surrounding region-who underwent outpatient examination in the winter of 2021 at Infectious Diseases Clinical Hospital No. 1 in Moscow (IKB No. 1) and medical institutions of the Moscow Regional Research Institute named after M.F. Vladimirsky Regional Research Institute (MONIKI) conducted a study without any connection to WNV infection in the summer-autumn of 2021.

Results: All samples were negative in ELISA-IgM test with WNV and TBEV antigens. In an ELISA-IgG test with the WNV antigen, antibodies were detected in 64 samples (4.0%). All samples were tested for IgG antibodies to TBEV in an ELISA and neutralizing antibodies to WNV and TBEV in a 50% plaque reduction neutralization test. Specific antibodies to WNV were detected in 44 samples (68.8%), to TBEV in eleven (17.2%), and group-specific antibodies in nine (14.0%). According to the total data from the test of 1,594 sera from residents of the Moscow region, specific antibodies to WNV were detected in 2.8% of cases, to TBEV in 0.7%, and group-specific antibodies in 0.6%. Ten of the 11 individuals with specific IgG antibodies to TBEV were undergoing outpatient examination at Moscow's Infectious Diseases Clinical Hospital No. 1 for a history of tick-borne encephalitis or for post-vaccination immunity testing following vaccination against this infection. The detection rate of specific antibodies to WNV in similar studies conducted in the same region in 2013 was 0.2%, while in 2021 it was 2.8%. The difference between these rates is statistically significant (p < 0.01).

Conclusion: Based on these data, it can be concluded that sporadic undiagnosed cases of WNV infection occurred in Moscow and the surrounding region between 2013 and 2021.

查看原文本刊更多论文

2021年西尼罗热疫情结束后对莫斯科和莫斯科地区居民的血清检查结果。

本研究的目的是在2021年10月疫情结束后，确定莫斯科地区人群对西尼罗病毒（WNV）的体液免疫水平，并通过使用ELISA-IgM、ELISA-IgG和50%斑块减少中和试验对患者血清中抗原性相关的地方性蜱传脑炎正黄病毒（TBEV）抗体进行比较检测，确认西尼罗病毒抗体的特异性。材料和方法：我们分析了2021年冬季在莫斯科第一传染病临床医院（IKB No. 1）和莫斯科地区研究所的医疗机构进行门诊检查的莫斯科及周边地区门诊患者的1594份血清，这些患者在2021年夏秋进行了一项与WNV感染无关的研究。结果：所有样本的WNV和TBEV抗原ELISA-IgM试验均为阴性。在WNV抗原的ELISA-IgG检测中，64份样本（4.0%）检测到抗体。所有样本均在ELISA中检测TBEV IgG抗体，并在50%斑块减少中和试验中检测WNV和TBEV的中和抗体。检测到WNV特异性抗体44例（68.8%），TBEV特异性抗体11例（17.2%），群体特异性抗体9例（14.0%）。根据莫斯科地区1594份居民血清检测的总数据，在2.8%的病例中检测到西尼罗河病毒特异性抗体，在0.7%的病例中检测到TBEV抗体，在0.6%的病例中检测到群体特异性抗体。在11名携带TBEV特异性IgG抗体的患者中，有10人在莫斯科第一传染病临床医院接受门诊检查，以确定是否有蜱传脑炎病史，或在接种疫苗后进行免疫检测。2013年在同一地区开展的类似研究中，西尼罗河病毒特异性抗体的检出率为0.2%，而2021年为2.8%。两组间差异有统计学意义（p < 0.01）。结论：2013 - 2021年，莫斯科及周边地区出现了散发的未确诊西尼罗河病毒感染病例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Voprosy virusologii Medicine-Infectious Diseases

CiteScore

2.00

自引率

0.00%

发文量

期刊介绍： The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.