Detection of Yezo virus (Nairoviridae, Orthonairovirus, Orthonairovirus yezoense) circulation in Ixodes persulcatus ticks in the Amur Region, Russia.

Q3 Medicine

Voprosy virusologii Pub Date : 2026-02-28 DOI:10.36233/0507-4088-358

M Y Kartashov, V Y Kurushina, A S Zheleznova, K A Svirin, E I Materikina, V A Ternovoi

{"title":"Detection of Yezo virus (Nairoviridae, Orthonairovirus, Orthonairovirus yezoense) circulation in Ixodes persulcatus ticks in the Amur Region, Russia.","authors":"M Y Kartashov, V Y Kurushina, A S Zheleznova, K A Svirin, E I Materikina, V A Ternovoi","doi":"10.36233/0507-4088-358","DOIUrl":null,"url":null,"abstract":"Introduction: The unfavorable epidemiological situation with viral tick-borne infections can be characterized not only by an increase in the incidence of «classical», well-known diseases, but also by the discovery of new pathogens, the role of which remains poorly understood. Yezo virus (YEZV) belongs to the group of recently discovered tick-borne nairoviruses. YEZV circulation is characteristic of Japan, China, and a number of regions in Russia (Primorsky, Khabarovsk, and Zabaykalsky Krais; Tomsk Oblast). The aim of the study was to screen and characterize the molecular genetics of YEZV isolated from ticks in the Amur region.Materials and methods: In the study, 704 individual tick samples (463 I. persulcatus ticks and 241 H. concinna ticks) collected from vegetation in 15 locations in 5 districts of the Amur Region were analyzed for the presence of YEZV RNA. Full-length genomic sequencing of the identified YEZV genetic variant was performed by preliminary enrichment of YEZV cDNA using multiplex PCR with a primer panel developed by us.Results: The YEZV infection rate in the studied sample of I. persulcatus ticks was 0.2% (1/463; 95% CI: 0.1-1.2).Conclusion: The results of this study expand our understanding of the spread of YEZV in Russia. Up-to-date information on the circulation and genetic diversity of nairoviruses that pose a potential threat to humans and animals contributes to the regulation and adjustment of preventive and anti-epidemic measures, as well as the implementation of adequate region-oriented laboratory diagnostics of IPK in the Far East, and especially in areas bordering China.","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"71 1","pages":"53-61"},"PeriodicalIF":0.0000,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Voprosy virusologii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36233/0507-4088-358","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: The unfavorable epidemiological situation with viral tick-borne infections can be characterized not only by an increase in the incidence of «classical», well-known diseases, but also by the discovery of new pathogens, the role of which remains poorly understood. Yezo virus (YEZV) belongs to the group of recently discovered tick-borne nairoviruses. YEZV circulation is characteristic of Japan, China, and a number of regions in Russia (Primorsky, Khabarovsk, and Zabaykalsky Krais; Tomsk Oblast). The aim of the study was to screen and characterize the molecular genetics of YEZV isolated from ticks in the Amur region.

Materials and methods: In the study, 704 individual tick samples (463 I. persulcatus ticks and 241 H. concinna ticks) collected from vegetation in 15 locations in 5 districts of the Amur Region were analyzed for the presence of YEZV RNA. Full-length genomic sequencing of the identified YEZV genetic variant was performed by preliminary enrichment of YEZV cDNA using multiplex PCR with a primer panel developed by us.

Results: The YEZV infection rate in the studied sample of I. persulcatus ticks was 0.2% (1/463; 95% CI: 0.1-1.2).

Conclusion: The results of this study expand our understanding of the spread of YEZV in Russia. Up-to-date information on the circulation and genetic diversity of nairoviruses that pose a potential threat to humans and animals contributes to the regulation and adjustment of preventive and anti-epidemic measures, as well as the implementation of adequate region-oriented laboratory diagnostics of IPK in the Far East, and especially in areas bordering China.

查看原文本刊更多论文

俄罗斯阿穆尔河地区过溶性硬蜱中叶虫病毒（奈罗病毒科、正虫病毒、正虫病毒叶虫属）传播的检测。

导言：病毒性蜱传感染的不利流行病学形势不仅表现为“经典”、众所周知的疾病发病率的增加，而且还表现为新病原体的发现，这些病原体的作用仍然知之甚少。叶佐病毒（YEZV）属于最近发现的蜱传奈罗病毒。YEZV环流是日本、中国和俄罗斯一些地区（滨海、哈巴罗夫斯克和扎巴卡尔斯基边疆区、托木斯克州）的特征。本研究的目的是筛选和鉴定阿穆尔河地区蜱分离的叶氏病毒的分子遗传学特征。材料与方法：本研究在黑龙江地区5个区15个地点的植被上采集704份蜱类标本（过狭蜱463份、细蜱241份），分析其YEZV RNA的存在情况。利用本研究开发的引物面板，利用多重PCR技术对鉴定的YEZV基因变异进行了初步富集，并对其进行了全基因组测序。结果：研究样本中过硫蜱YEZV感染率为0.2% （1/463,95% CI: 0.1 ~ 1.2）。结论：本研究结果扩大了我们对叶兹病毒在俄罗斯传播的认识。关于对人类和动物构成潜在威胁的奈罗病毒的传播和遗传多样性的最新信息有助于管理和调整预防和抗流行病措施，以及在远东，特别是在与中国接壤的地区，对IPK实施适当的面向区域的实验室诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Voprosy virusologii Medicine-Infectious Diseases

CiteScore

2.00

自引率

0.00%

发文量

期刊介绍： The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.