Virology最新文献_第9页

Complex interplay: The interactions between citrus tristeza virus and its host 复杂相互作用：柑橘tristeza病毒与其宿主之间的相互作用。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110388

Maryam Khalilzadeh , Dirk Jacobus Aldrich , Hans Jacob Maree , Amit Levy

引用次数: 0

Expression and purification of PNGase F protein in yeast and its anti-PRV activity PNGase F蛋白在酵母中的表达纯化及其抗prv活性研究。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2025.110393

Haonan Zhang , Yu Jiang , Gang Ding , Jingyu Chen , Yuda Liu , Furong Wang , Xiaolan Yu

{"title":"Expression and purification of PNGase F protein in yeast and its anti-PRV activity","authors":"Haonan Zhang , Yu Jiang , Gang Ding , Jingyu Chen , Yuda Liu , Furong Wang , Xiaolan Yu","doi":"10.1016/j.virol.2025.110393","DOIUrl":"10.1016/j.virol.2025.110393","url":null,"abstract":"<div><div>Pseudorabies virus (Pseudorabiesvirus, PRV) has caused huge economic losses to the global pig industry. In recent years, it has been reported that there are PRV mutants, but the traditional vaccine can not completely prevent or control the infection of PRV, so there is an urgent need to develop new broad-spectrum anti-disease drugs for prevention and treatment. PNGase F from bacteria can catalyze the hydrolysis of oligosaccharides linked to asparagine residues on peptides, so we speculate that PNGase F can inhibit virus infection by removing the glycosylation of virus membrane glycoproteins. In this study, PNGase F protein was highly expressed and purified in Pichia pastoris, and the deglycosylation activity of PNGase F expressed in Pichia pastoris was verified. In vitro, 15 μM could significantly inhibit the proliferation of virus in cells. The results of cytotoxicity test showed that PNGase F was not toxic to many cells. To further evaluate the effect of PNGase F in different stages of virus infection, it was found that PNGase F had significant inhibitory effect on virus adsorption and invasion. In vivo experiments in mice, PNGase F could significantly inhibit the replication of PRV Ea strain in mice and inhibit PRV, reduced brain lesions. Our experiments show that PNGase F expressed by yeast can inhibit PRV infection in vitro and in vitro, and its inhibitory mechanism is preliminarily discussed, which can provide a new reference for the development of broad-spectrum antiviral drugs based on PNGase F.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110393"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STAT1 and herpesviruses: Making lemonade from lemons

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110364

Erika R. Johansen, Vera L. Tarakanova

引用次数: 0

Deciphering the hepatitis E virus ORF1: Functional domains, protein processing, and patient-derived mutations 解密戊型肝炎病毒 ORF1：功能域、蛋白质加工和源自患者的突变。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110350

Fei Zhang , Ling-Dong Xu , Shiying Wu , Bin Wang , Pinglong Xu , Yao-Wei Huang

引用次数: 0

Intra-host variability of SARS-CoV-2: Patterns, causes and impact on COVID-19 SARS-CoV-2 的宿主内变异：模式、原因和对 COVID-19 的影响。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110366

Leandro R. Jones

引用次数: 0

LEF3 phosphorylation attenuates the replication of Bombyx mori nucleopolyhedrovirus by suppressing its interaction with alkaline nuclease LEF3磷酸化通过抑制家蚕核多角体病毒与碱性核酸酶的相互作用而减弱其复制。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110369

Chaoguang Gu , Yuqian Mo , Jiaqi Li , Xizhen Zhang , Siqi Xu , Meng Miao , Yanping Quan , Wei Yu

{"title":"LEF3 phosphorylation attenuates the replication of Bombyx mori nucleopolyhedrovirus by suppressing its interaction with alkaline nuclease","authors":"Chaoguang Gu , Yuqian Mo , Jiaqi Li , Xizhen Zhang , Siqi Xu , Meng Miao , Yanping Quan , Wei Yu","doi":"10.1016/j.virol.2024.110369","DOIUrl":"10.1016/j.virol.2024.110369","url":null,"abstract":"<div><div>Late expression factor 3 (LEF3), a multifunctional single-stranded DNA binding protein encoded by baculoviruses, is indispensable for viral DNA replication and plays a pivotal role in viral infection. Our previous quantitative analysis of phosphorylomics revealed that the phosphorylation levels of two serine residues (S8 and S25) located in LEF3 nuclear localization sequence were significantly up-regulated after <em>Bombyx mori</em> nucleopolyhedrovirus (BmNPV) infection, but the underlying mechanism remained unknown. To investigate the impact of phosphorylation on BmNPV infection, site-direct mutagenesis was performed on LEF3 to obtain phosphorylated mimic (S/D) or dephosphorylated mimic (S/A) mutants. The results demonstrated that the viral replication and proliferation were inhibited by phosphorylation of S8 or S25. Furthermore, we found that the N-terminal 125 amino acids region was responsible for interacting with virus-encoded alkaline nuclease, but this interaction could be suppressed by the phosphorylation. Our findings indicated that phosphorylation may serve as an antiviral strategy for host.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110369"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UL24 herpesvirus determinants of pathogenesis: Roles in virus-host interactions

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110376

Angela Pearson, Amel Bouhamar

引用次数: 0

(R)evolution of Viruses: Introduction to biothermodynamics of viruses (R)病毒的进化：病毒生物热力学导论。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110319

Marko E. Popović , Vojin Tadić , Marta Popović

引用次数: 0

Serologic differentiation between wild-type and cell-adapted African swine fever virus infections: A novel DIVA strategy using the MGF100-1L protein 野生型和细胞适应型非洲猪瘟病毒感染的血清学分化：使用MGF100-1L蛋白的一种新的DIVA策略

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110349

Theeradej Thaweerattanasinp, Janya Saenboonrueng, Asawin Wanitchang, Kanjana Srisutthisamphan, Nathiphat Tanwattana, Ratchanont Viriyakitkosol, Challika Kaewborisuth, Anan Jongkaewwattana

{"title":"Serologic differentiation between wild-type and cell-adapted African swine fever virus infections: A novel DIVA strategy using the MGF100-1L protein","authors":"Theeradej Thaweerattanasinp, Janya Saenboonrueng, Asawin Wanitchang, Kanjana Srisutthisamphan, Nathiphat Tanwattana, Ratchanont Viriyakitkosol, Challika Kaewborisuth, Anan Jongkaewwattana","doi":"10.1016/j.virol.2024.110349","DOIUrl":"10.1016/j.virol.2024.110349","url":null,"abstract":"<div><div>African swine fever virus (ASFV) poses a significant threat to the global swine industry and requires improved control strategies. Here, we developed a Differentiating Infected from Vaccinated Animals (DIVA) assay based on the MGF100-1L protein, which is absent in a cell-adapted ASFV strain lacking several multigene family (MGF) genes. We analyzed seven deleted genes, including MGF genes, from the right variable region of the ASFV genome against sera from convalescent pigs. MGF100-1L showed significant reactivity and was produced as a recombinant protein for use in an enzyme-linked immunosorbent assay (ELISA). The assay, with a cut-off value of 0.284, successfully differentiated between naive and infected pigs with 100% accuracy. More importantly, pigs infected with the cell-adapted ASFV showed no significant change in ELISA readouts after 27 days post-infection. However, when these pigs were subsequently challenged with wild-type virus, MGF100-1L reactivity increased significantly by 21 days post-challenge. This study demonstrates the potential of MGF100-1L as a DIVA marker for ASFV, which offers a promising tool to distinguish between infections with wild-type ASFV and those with cell-adapted variants lacking specific MGF genes, thereby improving ASFV surveillance and control strategies.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110349"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and molecular characterization of an enteric isolate of the genotype-Ia bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein 基因型-Ia 牛冠状病毒肠道分离株的分离和分子鉴定，该分离株在尖峰糖蛋白的受体结合域有显著突变。

IF 2.8 3区医学

Virology Pub Date : 2025-02-01 DOI: 10.1016/j.virol.2024.110313

Abid Ullah Shah , Phillip Gauger , Maged Gomaa Hemida

{"title":"Isolation and molecular characterization of an enteric isolate of the genotype-Ia bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein","authors":"Abid Ullah Shah , Phillip Gauger , Maged Gomaa Hemida","doi":"10.1016/j.virol.2024.110313","DOIUrl":"10.1016/j.virol.2024.110313","url":null,"abstract":"<div><div>BCoV new isolate was plaque purified, isolated, and propagated <em>in vitro</em> using MDBK and HRT-18. The full-length genome sequencing of this new BCoV isolate (31 Kbs) was drafted and deported in the GenBank. The genome organization is (5′-UTR-Gene-1-32kDa-HE-S-4.9 kDa-4.8 kDa-12.7 kDa-E-M-N-UTR-3′). Phylogenetic analysis based on the sequences of (the full-length genome, S, HE, and N) showed that the BCoV-13 clustered with other North American BCoV genotype I members. The sequence analysis shows several synonymous mutations among various domains of the S glycoprotein, especially the receptor binding domain. We found nine notable nucleotide deletions immediately downstream of the RNA binding domain of the nucleocapsid gene. Further gene function studies are encouraged to study the function of these mutations on the BCoV molecular pathogenesis and immune regulation. This research enhances our understanding of BCoV genomics and contributes to improved diagnostic and control measures for BCoV infections in cattle.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110313"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0