Veterinary Research最新文献_第4页

Carp edema virus surveillance in the koi trade: early detection through shipping environment sampling and longitudinal monitoring of CEV outbreaks in a wholesaler facility.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-03-04 DOI: 10.1186/s13567-025-01476-1

Laetitia Montacq, Marine Baud, Hélène Giummarra, Doriana Flores, Laurane Pallandre, Cécile Caubet, Sokunthea Top, Timothée Vergne, Laurent Bigarré, Stéphane Bertagnoli

{"title":"Carp edema virus surveillance in the koi trade: early detection through shipping environment sampling and longitudinal monitoring of CEV outbreaks in a wholesaler facility.","authors":"Laetitia Montacq, Marine Baud, Hélène Giummarra, Doriana Flores, Laurane Pallandre, Cécile Caubet, Sokunthea Top, Timothée Vergne, Laurent Bigarré, Stéphane Bertagnoli","doi":"10.1186/s13567-025-01476-1","DOIUrl":"10.1186/s13567-025-01476-1","url":null,"abstract":"Carp edema virus (CEV), a member of the Poxviridae family, has been a significant pathogen in koi and common carp since its initial identification in Japan during the 1970s. CEV, the causative agent of Koi Sleepy Disease (KSD), can cause high mortality rates and has been reported in many countries and is often linked to the fish trade. The virus is typically detected through DNA analysis of gill tissues, where the highest viral loads are found. However, traditional sampling methods, such as gill sampling, are lethal, complicating routine surveillance, particularly in asymptomatic or high-value koi. This study aimed to evaluate nonlethal sampling methods for CEV surveillance in the koi trade. We analysed various shipping environment samples, such as shipping water and fish bag swabs, alongside gill swabs from anaesthetised fish and gills from naturally deceased fish. Using qPCR, we found that the sensitivity of environmental samples, particularly shipping water, was greater than that of direct fish samples. Latent class modelling estimated that the sensitivity associated with 1.5 mL shipping water samples was greater than 89%, making them a reliable alternative for early detection. All detected variants belonged to genogroup II. Some post-import outbreaks shared variants with earlier outbreaks or shipping environment samples, suggesting that the detected DNA generally reflected infectious particles rather than just free environmental DNA and indicating that CEV can go unnoticed for several months after importation. These findings highlight the utility of environmental samples for effective, non-invasive surveillance and improved biosecurity management in the koi trade.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"48"},"PeriodicalIF":3.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piggybacking on nature: exploring the multifaceted world of porcine β-defensins.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-03-03 DOI: 10.1186/s13567-025-01465-4

Arthur Nery Finatto, François Meurens, Matheus de Oliveira Costa

引用次数: 0

The Seneca Valley virus 3C protease cleaves DCP1A to attenuate its antiviral effects.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-28 DOI: 10.1186/s13567-025-01477-0

Jingjing Yang, Zijian Li, Ruiyi Ma, Shijie Xie, Dan Wang, Rong Quan, Xuexia Wen, Jiangwei Song

{"title":"The Seneca Valley virus 3C protease cleaves DCP1A to attenuate its antiviral effects.","authors":"Jingjing Yang, Zijian Li, Ruiyi Ma, Shijie Xie, Dan Wang, Rong Quan, Xuexia Wen, Jiangwei Song","doi":"10.1186/s13567-025-01477-0","DOIUrl":"10.1186/s13567-025-01477-0","url":null,"abstract":"Seneca Valley virus (SVV), a new member of Picornaviridae, causes idiopathic vesicular symptoms in pregnant sows and acute death in neonatal piglets, considerably damaging the swine industry. The viral protease 3C (3Cpro) cleaves host immune-related molecules to create a favorable environment for viral replication. In this study, we found that mRNA decapping enzyme 1A (DCP1A) is a novel antiviral effector against SVV infection that targets 3D viral RNA-dependent RNA polymerase for OPTN-mediated autophagic degradation. To counteract this effect, SVV 3Cpro targets DCP1A for cleavage at glutamine 343 (Q343), resulting in the cleaved products DCP1A (1-343) and DCP1A (344-580), which lose the ability to restrict SVV replication. In contrast, the 3C cleavage-resistant DCP1A-Q343A mutant exhibited stronger antiviral effects than the wild-type DCP1A. Additionally, the degradation of the viral 3D protein targeted by DCP1A was abolished after its cleavage by SVV 3Cpro. In conclusion, our study demonstrated that SVV 3Cpro is a pivotal ISG antagonist that cleaves DCP1A. These results offer novel insight into how viruses evade host immunity.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"46"},"PeriodicalIF":3.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HnRNPC triggers the degradation of MITA to suppress the interferon-mediated antiviral response.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-24 DOI: 10.1186/s13567-025-01463-6

Yanwei Zhang, Zhao Jia, Gaoliang Yuan, Kangyong Chen, Jing Cen, Junya Wang, Hao Feng, Mikolaj Adamek, Jun Zou

{"title":"HnRNPC triggers the degradation of MITA to suppress the interferon-mediated antiviral response.","authors":"Yanwei Zhang, Zhao Jia, Gaoliang Yuan, Kangyong Chen, Jing Cen, Junya Wang, Hao Feng, Mikolaj Adamek, Jun Zou","doi":"10.1186/s13567-025-01463-6","DOIUrl":"10.1186/s13567-025-01463-6","url":null,"abstract":"Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a group of 34-120 kDa nuclear proteins that have recently been reported to participate in virus replication. The hnRNP family contains approximately 20 members, including hnRNP A1, hnRNP A2, hnRNP A2B1, hnRNPC, hnRNPD and hnRNPK. HnRNPC plays important roles in RNA biology, including expression, stability, mRNA splicing, nonspecific sequence export and 3'-end processing; however, the mechanisms underlying hnRNPC regulatory roles are not fully understood. Here, we found that zebrafish hnRNPC promoted spring viraemia of carp virus (SVCV) replication by increasing the stability of SVCV phosphoprotein while inhibiting the K48-linked ubiquitination of virus phosphoprotein, thereby suppressing the type I interferon (IFN) response. Mechanistically, hnRNPC could interact with the mediator of IFN regulatory factor 3 activation (MITA) to activate K48-linked ubiquitination for MITA degradation through the C-terminal domain of hnRNPC. We also showed that human hnRNPC could interact with MITA and that the overexpression of human hnRNPC decreased MITA protein in HEK293 cells, suggesting that the negative regulatory effects of hnRNPC on the type I IFN response are evolutionarily conserved. Collectively, our data indicate that hnRNPC promotes virus replication by suppressing IFN production activated by MITA and increasing the availability of viral proteins. Our work reveals an evolutionarily conserved mechanism that controls the IFN-mediated antiviral response by a member of the hnRNP family in vertebrates.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"45"},"PeriodicalIF":3.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Usutu virus continues to spread across Europe: first report of multiple molecular detections of the USUV Africa 2 and Africa 3 lineages in free-living and captive birds in Poland, July-November 2023.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-17 DOI: 10.1186/s13567-025-01460-9

Kamila Dziadek, Jowita Samanta Niczyporuk, Natalia Styś-Fijoł, Agnieszka Czujkowska, Krzysztof Śmietanka, Katarzyna Domańska-Blicharz

引用次数: 0

MoxR effects as an ATPase on anti-stress and pathogenicity of Riemerella anatipestifer.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-17 DOI: 10.1186/s13567-025-01454-7

Yang Zhang, Yanhao Zhang, Yushan He, Yarong Hou, Xuedi Li, Xueying Yang, Zutao Zhou, Zili Li

{"title":"MoxR effects as an ATPase on anti-stress and pathogenicity of Riemerella anatipestifer.","authors":"Yang Zhang, Yanhao Zhang, Yushan He, Yarong Hou, Xuedi Li, Xueying Yang, Zutao Zhou, Zili Li","doi":"10.1186/s13567-025-01454-7","DOIUrl":"10.1186/s13567-025-01454-7","url":null,"abstract":"Duck infectious serositis is a septicemic disease caused by the bacterium Riemerella anatipestifer (R. anatipestifer), which affects ducks, geese, turkeys, and other poultry. While outbreaks have been reported worldwide, the exact mechanisms of infection and disease progression remain unclear. Our previous research identified the two-component system PhoPR within the genome of R. anatipestifer and demonstrated its association with the bacterium's pathogenicity. Through multi-omics analysis, we found that PhoP directly regulates the expression of several genes, including moxR, within the Bacteroides aerotolerance (Bat) operon. However, the function of MoxR in R. anatipestifer has not yet been reported. To investigate the impact of MoxR on the expression of the bat operon and the pathogenicity of R. anatipestifer, we constructed ΔmoxR and other derivative strains. Our findings revealed that overexpression of MoxR inhibits the transcription of the bat operon. Conversely, deletion of moxR, along with exposure of R. anatipestifer to thermal or oxidative stress, results in increased transcription levels of the bat operon. By measuring the survival ability of each strain under stress, we discovered that MoxR is closely associated with the resistance of R. anatipestifer to thermal and oxidative stress by influencing the expression of the bat operon. Duckling infection experiments, along with adhesion and invasion assays, showed that deletion of moxR in R. anatipestifer led to decreased pathogenicity, and lower bacterial load in various tissues. Collectively, our findings collectively demonstrate the significant role of MoxR in the anti-stress and pathogenicity of R. anatipestifer, providing new insights into its pathogenic mechanisms.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"44"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BEV 2C protein inhibits the NF-κB signalling pathway to promote viral replication by targeting IKBKB and p65.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-16 DOI: 10.1186/s13567-025-01453-8

Xuyuan Cui, Yidi Guo, Fan Zhang, Xiaoran Chang, Junying Hu, Qun Zhang, Xuebo Zheng, NaiTian Yan, Xinping Wang

{"title":"BEV 2C protein inhibits the NF-κB signalling pathway to promote viral replication by targeting IKBKB and p65.","authors":"Xuyuan Cui, Yidi Guo, Fan Zhang, Xiaoran Chang, Junying Hu, Qun Zhang, Xuebo Zheng, NaiTian Yan, Xinping Wang","doi":"10.1186/s13567-025-01453-8","DOIUrl":"10.1186/s13567-025-01453-8","url":null,"abstract":"Bovine enterovirus, a member of the Enterovirus genus in the Picornaviridae family, causes severe digestive and respiratory illnesses in cattle. These illnesses threaten the healthy development of the cattle industry. Innate immunity plays a critical role in resisting viral infections, but viruses also use various strategies to evade or counteract the host's immune system. The mechanisms by which bovine enteroviruses evade the host immune response and promote their replication remain unclear. This study used the HY12 strain of enterovirus as a model to investigate its interaction with both bovine enterovirus and its host. Our findings indicate that bovine enterovirus promotes the replication of HY12 by disrupting the NF-κB pathway. Here, one strategy was to down-regulate the IΚBΚB expression to inhibit the activation of NF-κB. Another approach was to directly interact with p65 to reduce the dimerisation of p65/p50 and inhibit the phosphorylation and nuclear translocation of p65. Our study's results show that 2C's N-terminal 1-121 aa is essential for 2C-mediated inhibition of the NF-κB signalling pathway, and four amino acids (position 118-121 aa) are the interaction site of 2C with p65. This report is the first on BEV 2C protein promoting virus replication through new strategies, which provides novel insights into the understanding of enterovirus pathobiology and the development of drugs against BEV.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"42"},"PeriodicalIF":3.7,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11831767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study in the iron uptake mechanism of Pasteurella multocida.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-13 DOI: 10.1186/s13567-025-01469-0

Xiangxiang Shen, Lijun Guan, Junfeng Zhang, Yun Xue, Lifang Si, Zhanqin Zhao

引用次数: 0

Development of a novel chimeric lysin to combine parental phage lysin and cefquinome for preventing sow endometritis after artificial insemination.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-11 DOI: 10.1186/s13567-025-01457-4

Xin-Xin Li, Zi-Qiang Hong, Zhi-Xuan Xiong, Li-Wen Zhang, Shuang Wang, Pan Tao, Pin Chen, Xiang-Min Li, Ping Qian

{"title":"Development of a novel chimeric lysin to combine parental phage lysin and cefquinome for preventing sow endometritis after artificial insemination.","authors":"Xin-Xin Li, Zi-Qiang Hong, Zhi-Xuan Xiong, Li-Wen Zhang, Shuang Wang, Pan Tao, Pin Chen, Xiang-Min Li, Ping Qian","doi":"10.1186/s13567-025-01457-4","DOIUrl":"10.1186/s13567-025-01457-4","url":null,"abstract":"Sow endometritis is usually caused by multiple species of pathogenic bacteria. Numerous isolates from endometritis patients have developed antimicrobial resistance. Thus, novel antibacterial agents and strategies to combat endometritis are needed. A total of 526 bacteria, including Staphylococcus spp. (26.3%), Streptococcus spp. (12.3%), E. coli (28.9%), Enterococcus spp. (20.1%), Proteus spp. (9.5%), and Corynebacterium spp. (2.8%), were isolated from sows with endometritis. We constructed a novel chimeric lysin, ClyL, which is composed of a cysteine- and histidine-dependent amidohydrolase/peptidase (CHAP) catalytic domain from the phage lysin LysGH15 and a cell wall-binding domain (CBD) from the prophage lysin Lys0859. The activities of ClyL and Lys0859 were most pronounced for the Staphylococcus and Streptococcus strains isolated from sow endometritis and bovine mastitis, respectively. ClyL and Lys0859 were combined to create a phage lysin cocktail, which demonstrated a synergistic effect against the coinfection of Staphylococcus and Streptococcus in vitro and in vivo. Furthermore, the combination of phage lysin cocktail and cefquinome had a synergistic bactericidal effect on boar semen that did not influence the activity of sperm. Remarkably, the incidence rate of sow endometritis was 0% (0/7) when the combination of phage lysin cocktail and cefquinome was used in semen via artificial insemination compared with 50% (3/6) when PBS was administered. Overall, the administration of a phage lysin cocktail and cefquinome in semen via artificial insemination is a promising novel strategy to prevent sow endometritis after artificial insemination.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"39"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Opportunistic pathogens are prevalent across the culturable exogenous and endogenous microbiota of stable flies captured at a dairy facility.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-11 DOI: 10.1186/s13567-025-01458-3

Andrew J Sommer, Courtney L Deblois, Andrew D J Tu, Garret Suen, Kerri L Coon

{"title":"Opportunistic pathogens are prevalent across the culturable exogenous and endogenous microbiota of stable flies captured at a dairy facility.","authors":"Andrew J Sommer, Courtney L Deblois, Andrew D J Tu, Garret Suen, Kerri L Coon","doi":"10.1186/s13567-025-01458-3","DOIUrl":"10.1186/s13567-025-01458-3","url":null,"abstract":"Stable flies in the genus Stomoxys are highly abundant, blood-feeding pests on dairy farms; however, their role in the carriage and potential transmission of pathogens is largely understudied. Here, we report on the frequency and distribution of culturable bacteria collected from Stomoxys flies captured in free stall barns and nearby calf hutches over a three-month period on a focal research farm in Wisconsin, USA. Mastitis-associated bacterial taxa, including Staphylococcus, Escherichia, Enterobacter, and Klebsiella spp., were frequently isolated from pooled samples of the internal or external portions of the flies. Conversely, selective enrichment protocols from these samples yielded only a single isolate of Salmonella and no enterohemorrhagic Escherichia coli O157. Neither trap location nor time of capture had a significant impact on the observed frequency of most bacterial genera isolated from the flies. Our results confirm that Stomoxys flies harbor both mastitis-associated bacterial taxa and bacterial taxa associated with opportunistic infections in humans. Further research into the transmission of fly-associated microbes could be important in the control of mastitis or other bacterial diseases on dairy farms.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"40"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11817017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0