Veterinary Research最新文献_第4页

Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research. 为抗病毒研究开发猫传染性腹膜炎病毒反向遗传学系统并确定其特征。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01373-z

Guoqian Gu, To Sing Fung, Wong Tsz Hung, Nikolaus Osterrieder, Yun Young Go

{"title":"Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research.","authors":"Guoqian Gu, To Sing Fung, Wong Tsz Hung, Nikolaus Osterrieder, Yun Young Go","doi":"10.1186/s13567-024-01373-z","DOIUrl":"https://doi.org/10.1186/s13567-024-01373-z","url":null,"abstract":"Feline infectious peritonitis (FIP) is a lethal, immune-mediated disease in cats caused by feline infectious peritonitis virus (FIPV), a biotype of feline coronavirus (FCoV). In contrast to feline enteric coronavirus (FECV), which exclusively infects enterocytes and causes diarrhea, FIPV specifically targets macrophages, resulting in the development of FIP. The transmission and infection mechanisms of this complex, invariably fatal disease remain unclear, with no effective vaccines or approved drugs for its prevention or control. In this study, a full-length infectious cDNA clone of the wild-type FIPV WSU79-1149 strain was constructed to generate recombinant FIPV (rFIPV-WT), which exhibited similar growth kinetics and produced infectious virus titres comparable to those of the parental wild-type virus. In addition, the superfold green fluorescent protein (msfGFP) and Renilla luciferase (Rluc) reporter genes were incorporated into the rFIPV-WT cDNA construct to generate reporter rFIPV-msfGFP and rFIPV-Rluc viruses. While the growth characteristics of the rFIPV-msfGFP virus were similar to those of its parental rFIPV-WT, the rFIPV-Rluc virus replicated more slowly, resulting in the formation of smaller plaques than did the rFIPV-WT and rFIPV-msfGFP viruses. In addition, by replacing the S, E, M, and ORF3abc genes with msfGFP and Rluc genes, the replicon systems repFIPV-msfGFP and repFIPV-Rluc were generated on the basis of the cDNA construct of rFIPV-WT. Last, the use of reporter recombinant viruses and replicons in antiviral screening assays demonstrated their high sensitivity for quantifying the antiviral effectiveness of the tested compounds. This integrated system promises to significantly streamline the investigation of virus replication within host cells, enabling efficient screening for anti-FIPV compounds and evaluating emerging drug-resistant mutations within the FIPV genome.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"124"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein. RSAD2 通过与塞内卡病毒 A 2 C 蛋白相互作用来抑制病毒复制。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01370-2

Lei Hou, Zhi Wu, Penghui Zeng, Xiaoyu Yang, Yongyan Shi, Jinshuo Guo, Jianwei Zhou, Jiangwei Song, Jue Liu

{"title":"RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein.","authors":"Lei Hou, Zhi Wu, Penghui Zeng, Xiaoyu Yang, Yongyan Shi, Jinshuo Guo, Jianwei Zhou, Jiangwei Song, Jue Liu","doi":"10.1186/s13567-024-01370-2","DOIUrl":"10.1186/s13567-024-01370-2","url":null,"abstract":"Senecavirus A (SVA), an emerging virus that causes blisters on the nose and hooves, reduces the production performance of pigs. RSAD2 is a radical S-adenosylmethionine (SAM) enzyme, and its expression can suppress various viruses due to its broad antiviral activity. However, the regulatory relationship between SVA and RSAD2 and the mechanism of action remain unclear. Here, we demonstrated that SVA infection increased RSAD2 mRNA levels, whereas RSAD2 expression negatively regulated viral replication, as evidenced by decreased viral VP1 protein expression, viral titres, and infected cell numbers. Viral proteins that interact with RSAD2 were screened, and the interaction between the 2 C protein and RSAD2 was found to be stronger than that between other proteins. Additionally, amino acids (aa) 43-70 of RSAD2 were crucial for interacting with the 2 C protein and played an important role in its anti-SVA activity. RSAD2 was induced by type I interferon (IFN-I) via Janus kinase signal transducer and activator of transcription (JAK-STAT), and had antiviral activity. Ruxolitinib, a JAK-STAT pathway inhibitor, and the knockdown of JAK1 expression substantially reduced RSAD2 expression levels and antiviral activity. Taken together, these results revealed that RSAD2 blocked SVA infection by interacting with the viral 2 C protein and provide a strategy for preventing and controlling SVA infection.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"115"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11430333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased viperin expression induced by avian infectious bronchitis virus inhibits viral replication by restricting cholesterol synthesis: an in vitro study. 禽传染性支气管炎病毒诱导的蝰蛇素表达增加，通过限制胆固醇合成抑制病毒复制：一项体外研究。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01368-w

Yu Zhang, Tao-Ni Zhang, Yan-Peng Lu, Li-Na Ren, Sheng-Ting Chen, Ling Liu, Lan-Ping Wei, Ji-Ming Chen, Jian-Ni Huang, Mei-Lan Mo

{"title":"Increased viperin expression induced by avian infectious bronchitis virus inhibits viral replication by restricting cholesterol synthesis: an in vitro study.","authors":"Yu Zhang, Tao-Ni Zhang, Yan-Peng Lu, Li-Na Ren, Sheng-Ting Chen, Ling Liu, Lan-Ping Wei, Ji-Ming Chen, Jian-Ni Huang, Mei-Lan Mo","doi":"10.1186/s13567-024-01368-w","DOIUrl":"10.1186/s13567-024-01368-w","url":null,"abstract":"With the emergence of new variant strains resulting from high mutation rates and genome recombination, avian infectious bronchitis virus (IBV) has caused significant economic losses to the poultry industry worldwide. Little is known about the underlying mechanisms of IBV-host interactions, particularly how IBV utilizes host metabolic pathways for efficient viral replication and transmission. In the present study, the effects of the cell membrane, viral envelope membrane, and viperin-mediated cholesterol synthesis on IBV replication were explored. Our results revealed significant increase in cholesterol levels and the expression of viperin after IBV infection. Acute cholesterol depletion in the cell membrane and viral envelope membrane by treating cells with methyl-β-cyclodextrin (MβCD) obviously inhibited IBV replication; thereafter, replenishment of the cell membrane with cholesterol successfully restored viral replication, and direct addition of exogenous cholesterol to the cell membrane significantly promoted IBV infection during the early stages of infection. In addition, overexpression of viperin effectively suppressed cholesterol synthesis, as well as IBV replication, whereas knockdown of viperin (gene silencing with siRNA targeting viperin, siViperin) significantly increased IBV replication and cholesterol levels, whereas supplementation with exogenous cholesterol to viperin-transfected cells markedly restored viral replication. In conclusion, the increase in viperin induced by IBV infection plays an important role in IBV replication by affecting cholesterol production, providing a theoretical basis for understanding the pathogenesis of IBV and discovering new potential antiviral targets.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"116"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11429478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RACK1 and NEK7 mediate GSDMD-dependent macrophage pyroptosis upon Streptococcus suis infection. RACK1 和 NEK7 在猪链球菌感染时介导 GSDMD 依赖性巨噬细胞热解。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01376-w

Xin Shen, Jinrong Ran, Qingqing Yang, Bingjie Li, Yi Lu, Jiajia Zheng, Liuyi Xu, Kaixiang Jia, Zhiwei Li, Lianci Peng, Rendong Fang

{"title":"RACK1 and NEK7 mediate GSDMD-dependent macrophage pyroptosis upon Streptococcus suis infection.","authors":"Xin Shen, Jinrong Ran, Qingqing Yang, Bingjie Li, Yi Lu, Jiajia Zheng, Liuyi Xu, Kaixiang Jia, Zhiwei Li, Lianci Peng, Rendong Fang","doi":"10.1186/s13567-024-01376-w","DOIUrl":"10.1186/s13567-024-01376-w","url":null,"abstract":"Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that induces an NLRP3-dependent cytokine storm. NLRP3 inflammasome activation triggers not only an inflammatory response but also pyroptosis. However, the exact mechanism underlying S. suis-induced macrophage pyroptosis is not clear. Our results showed that SS2 induced the expression of pyroptosis-associated factors, including lactate dehydrogenase (LDH) release, propidium iodide (PI) uptake and GSDMD-N expression, as well as NLRP3 inflammasome activation and IL-1β secretion. However, GSDMD deficiency and NLRP3 inhibition using MCC950 attenuated the SS2-induced expression of pyroptosis-associated factors, suggesting that SS2 induces NLRP3-GSDMD-dependent pyroptosis. Furthermore, RACK1 knockdown also reduced the expression of pyroptosis-associated factors. In addition, RACK1 knockdown downregulated the expression of NLRP3 and Pro-IL-1β as well as the phosphorylation of P65. Surprisingly, the interaction between RACK1 and P65 was detected by co-immunoprecipitation, indicating that RACK1 induces macrophage pyroptosis by mediating the phosphorylation of P65 to promote the transcription of NLRP3 and pro-IL-1β. Similarly, NEK7 knockdown decreased the expression of pyroptosis-associated factors and ASC oligomerization. Moreover, the results of co-immunoprecipitation revealed the interaction of NEK7-RACK1-NLRP3 during SS2 infection, demonstrating that NEK7 mediates SS2-induced pyroptosis via the regulation of NLRP3 inflammasome assembly and activation. These results demonstrate the important role of RACK1 and NEK7 in SS2-induced pyroptosis. Our study provides new insight into SS2-induced cell death.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"120"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11428613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characteristics associated with the virulence of porcine epidemic diarrhea virus (PEDV) with a naturally occurring truncated ORF3 gene. 带有天然截短 ORF3 基因的猪流行性腹泻病毒（PEDV）与毒力相关的遗传特征。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01384-w

Ying Lu, Weijian Huang, Zhengpu Lu, Deping Zeng, Kechen Yu, Jiaguo Bai, Qiuying Qin, Meijin Long, Yifeng Qin, Ying Chen, Zuzhang Wei, Kang Ouyang

{"title":"Genetic characteristics associated with the virulence of porcine epidemic diarrhea virus (PEDV) with a naturally occurring truncated ORF3 gene.","authors":"Ying Lu, Weijian Huang, Zhengpu Lu, Deping Zeng, Kechen Yu, Jiaguo Bai, Qiuying Qin, Meijin Long, Yifeng Qin, Ying Chen, Zuzhang Wei, Kang Ouyang","doi":"10.1186/s13567-024-01384-w","DOIUrl":"https://doi.org/10.1186/s13567-024-01384-w","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) has emerged in American countries, and it has reemerged in Asia and Europe, causing significant economic losses to the pig industry worldwide. In the present study, the 17GXCZ-1ORF3d strain, which has a naturally large deletion at the 172-554 bp position of the ORF3 gene, together with the 17GXCZ-1ORF3c strain, was serially propagated in Vero cells for up to 120 passages. The adaptability of the two strains gradually increased through serial passages in vitro. Genetic variation analysis of the variants of the two strains from different generations revealed that the naturally truncated ORF3 gene in the 17GXCZ-1ORF3d variants was stably inherited. Furthermore, the survival, viral shedding and histopathological lesions following inoculation of piglets demonstrated that the virulence of 17GXCZ-1ORF3d-P120 was significantly attenuated. These results indicate that the naturally truncated ORF3 gene may accelerate the attenuation of virulence and is involved in PEDV virulence together with mutations in other structural genes. Importantly, immunization of sows with G2b 17GXCZ-1ORF3d-P120 increased PEDV-specific IgG and IgA antibody levels in piglets and conferred partial passive protection against heterologous G2a PEDV strains. Our findings suggest that an attenuated strain with a truncated ORF3 gene may be a promising candidate for protection against PEDV.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"123"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11437794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LINC01197 inhibits influenza A virus replication by serving as a PABPC1 decoy. LINC01197 可作为 PABPC1 诱饵抑制甲型流感病毒的复制。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01379-7

Yihe Wang, Ning Shi, Hansi Zhang, Jinna Luo, Hongjian Yan, Huiyan Hou, Zhenhong Guan, Lili Zhao, Ming Duan

引用次数: 0

New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph. 利用第三代纳米孔测序技术对蜜蜂血淋巴的病毒和细菌季节性感染模式的新认识。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01382-y

Cato Van Herzele, Sieglinde Coppens, Nick Vereecke, Sebastiaan Theuns, Dirk C de Graaf, Hans Nauwynck

{"title":"New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph.","authors":"Cato Van Herzele, Sieglinde Coppens, Nick Vereecke, Sebastiaan Theuns, Dirk C de Graaf, Hans Nauwynck","doi":"10.1186/s13567-024-01382-y","DOIUrl":"https://doi.org/10.1186/s13567-024-01382-y","url":null,"abstract":"Honey bees are rapidly declining, which poses a significant threat to our environment and agriculture industry. These vital insects face a disease complex believed to be caused by a combination of parasites, viruses, pesticides, and nutritional deficiencies. However, the real aetiology is still enigmatic. Due to the conventional analysis methods, we still lack complete insights into the honey bee virome and the presence of pathogenic bacteria. To fill this knowledge gap, we employed third-generation nanopore metagenomic sequencing on honey bee haemolymph to monitor the presence of pathogens over almost a year. This study provides valuable insights into the changes in bacterial and viral loads within honey bee colonies. We identified different pathogens in the honey bee haemolymph, which are not included in honey bee screenings. These pathogens comprise the Apis mellifera filamentous virus, Apis rhabdoviruses, and various bacteria such as Frischella sp. and Arsenophonus sp. Furthermore, a sharp contrast was observed between young and old bees. Our research proposes that transgenerational immune priming may play a role in shaping infection patterns in honey bees. We observed a significant increase in pathogen loads in the spring, followed by a notable decrease in pathogen presence during the summer and autumn months. However, certain pathogens seem to be able to evade this priming effect, making them particularly intriguing as potential factors contributing to mortality. In the future, we aim to expand our research on honey bee transgenerational immune priming and investigate its potential in natural settings. This knowledge will ultimately enhance honey bee health and decrease colony mortality.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"118"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11430211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cadDX operon contributes to cadmium resistance, oxidative stress resistance, and virulence in zoonotic streptococci. cadDX 操作子有助于提高人畜共患链球菌的抗镉、抗氧化压力和毒力。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01371-1

Xinchi Zhu, Zijing Liang, Jiale Ma, Jinhu Huang, Liping Wang, Huochun Yao, Zongfu Wu

{"title":"The cadDX operon contributes to cadmium resistance, oxidative stress resistance, and virulence in zoonotic streptococci.","authors":"Xinchi Zhu, Zijing Liang, Jiale Ma, Jinhu Huang, Liping Wang, Huochun Yao, Zongfu Wu","doi":"10.1186/s13567-024-01371-1","DOIUrl":"10.1186/s13567-024-01371-1","url":null,"abstract":"Mobile genetic elements (MGEs) enable bacteria to acquire novel genes and traits. However, the functions of cargo genes within MGEs remain poorly understood. The cadmium resistance operon cadDX is present in many gram-positive bacteria. Although cadDX has been reported to be involved in metal detoxification, its regulatory mechanisms and functions in bacterial pathogenesis are poorly understood. This study revealed that cadDX contributes to cadmium resistance, oxidative stress resistance, and virulence in Streptococcus suis, an important zoonotic pathogen in pigs and humans. CadX represses cadD expression by binding to the cadDX promoter. Notably, cadX responds to H2O2 stress through an additional promoter within the cadDX operon, mitigating the harmful effect of excessive cadD expression during oxidative stress. cadDX resides within an 11 K integrative and mobilizable element that can autonomously form circular structures. Moreover, cadDX is found in diverse MGEs, accounting for its widespread distribution across various bacteria, especially among pathogenic streptococci. Transferring cadDX into another zoonotic pathogen, Streptococcus agalactiae, results in similar phenotypes, including resistance to cadmium and oxidative stresses and increased virulence of S. agalactiae in mice. The new functions and regulatory mechanisms of cadDX shed light on the importance of the cadDX system in driving evolutionary adaptations and survival strategies across diverse gram-positive bacteria.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"119"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11430099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142336656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Streptococcus suis isolates recovered from diseased pigs in Europe. 从欧洲病猪中分离出的猪链球菌的分子特征。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01366-y

Kevin Li, Sonia Lacouture, Eric Lewandowski, Eric Thibault, Hubert Gantelet, Marcelo Gottschalk, Nahuel Fittipaldi

{"title":"Molecular characterization of Streptococcus suis isolates recovered from diseased pigs in Europe.","authors":"Kevin Li, Sonia Lacouture, Eric Lewandowski, Eric Thibault, Hubert Gantelet, Marcelo Gottschalk, Nahuel Fittipaldi","doi":"10.1186/s13567-024-01366-y","DOIUrl":"https://doi.org/10.1186/s13567-024-01366-y","url":null,"abstract":"Streptococcus suis is a major swine pathogen and zoonotic agent, causing important economic losses to the porcine industry. Here, we used genomics approaches to characterize 251 S. suis isolates recovered from diseased pigs across Belgium, France, Germany, Hungary, the Netherlands, Spain, and the United Kingdom. We identified 13 serotypes, being serotypes 9 and 2 the most prevalent, and 34 sequence types (STs), including 16 novel STs, although ST16 and ST1 dominated the strain population. Phylogenetic analysis revealed complex genetic relationships, notable geographic clustering, and potential differential capacity for capsular switching among serotype 9 isolates. We found antimicrobial resistance (AMR) genes in 85.3% of the isolates, with high frequencies of genes conferring resistance to tetracyclines and macrolides. Specifically, 49.4% of the isolates harbored the tetO gene, and 64.9% possessed the ermB gene. Additionally, we observed a diverse array of virulence-associated genes (VAGs), including the classical VAGs mrp, epf, and sly, with variable presence across different genotypes. The high genetic diversity among European S. suis isolates highlights the importance of targeted antimicrobial use and flexible vaccine strategies. Rapid strain characterization is crucial for optimizing swine health management, enabling tailored interventions like the development of autovaccines to mitigate S. suis infections.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"117"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11429987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-transcriptome analyses of ovine lung microvascular endothelial cells infected with bluetongue virus. 对感染蓝舌病病毒的绵羊肺微血管内皮细胞进行全转录组分析。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01372-0

Shimei Luo, Yunyi Chen, Xianping Ma, Haisheng Miao, Huaijie Jia, Huashan Yi

{"title":"Whole-transcriptome analyses of ovine lung microvascular endothelial cells infected with bluetongue virus.","authors":"Shimei Luo, Yunyi Chen, Xianping Ma, Haisheng Miao, Huaijie Jia, Huashan Yi","doi":"10.1186/s13567-024-01372-0","DOIUrl":"https://doi.org/10.1186/s13567-024-01372-0","url":null,"abstract":"Bluetongue virus (BTV) infection induces profound and intricate changes in the transcriptional profile of the host to facilitate its survival and replication. However, there have been no whole-transcriptome studies on ovine lung microvascular endothelial cells (OLMECs) infected with BTV. In this study, we comprehensively analysed the whole-transcriptome sequences of BTV-1 serotype-infected and mock-infected OLMECs and subsequently performed bioinformatics differential analysis. Our analysis revealed 1215 differentially expressed mRNA transcripts, 82 differentially expressed long noncoding RNAs (lncRNAs) transcripts, 63 differentially expressed microRNAs (miRNAs) transcripts, and 42 differentially expressed circular RNAs (circRNAs) transcripts. Annotation from Gene Ontology, enrichment from the Kyoto Encyclopedia of Genes and Genomes, and construction of endogenous competing RNA network analysis revealed that the differentially expressed RNAs primarily participated in viral sensing and signal transduction pathways, antiviral and immune responses, inflammation, and extracellular matrix (ECM)-related pathways. Furthermore, protein‒protein interaction network analysis revealed that BTV may regulate the conformation of ECM receptor proteins and change their biological activity through a series of complex mechanisms. Finally, on the basis of real-time fluorescence quantitative polymerase chain reaction results, the expression trends of the differentially expressed RNA were consistent with the whole-transcriptome sequencing data, such as downregulation of the expression of COL4A1, ITGA8, ITGB5, and TNC and upregulation of the expression of CXCL10, RNASEL, IRF3, IRF7, and IFIHI. This study provides a novel perspective for further investigations of the mechanism of the ECM in the BTV-host interactome and the pathogenesis of lung microvascular endothelial cells.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"122"},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0