Indonesian Journal of Biotechnology and Biodiversity最新文献_第3页

Factor affecting public acceptance on Genetically Modified Food: A Review 影响公众对转基因食品接受程度的因素综述

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-12-31 DOI: 10.47007/ijobb.v5i3.92

A. A. Hermosaningtyas

引用次数: 0

Phytoplankton in Boom Beach, Banyuwangi Regency East Java : Their Existence as a Bioindicator of Water Saprobity 东爪哇Banyuwangi摄政的Boom Beach的浮游植物:它们作为水污染的生物指示物的存在

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-12-31 DOI: 10.47007/ijobb.v5i3.97

An Ayun, N. Nurwidodo, H. Husamah

引用次数: 0

Synthesis of Alginate Oligosaccharide (AOS) Using Different Solvents 不同溶剂合成海藻酸盐寡糖(AOS)

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-12-31 DOI: 10.47007/ijobb.v5i3.98

Nathaniel Nainggolan, Seprianto Seprianto, R. Kusumawati

{"title":"Synthesis of Alginate Oligosaccharide (AOS) Using Different Solvents","authors":"Nathaniel Nainggolan, Seprianto Seprianto, R. Kusumawati","doi":"10.47007/ijobb.v5i3.98","DOIUrl":"https://doi.org/10.47007/ijobb.v5i3.98","url":null,"abstract":"Alginate oligosaccharide (AOS) is an oligosaccharide synthesized by depolymerizing alginate and has various industrial applications. This research determines the best solvent and the optimal concentration of the best solvent to produce AOS. Peroxides and hydrochloric acid can be used[L1] as solvents, but the residue might cause some danger if the AOS extract is used for food or pharmaceutical purposes. Citric acid can be used as a solvent, but it is necessary to determine the optimum concentration to synthesize AOS. Citric acid concentration variations are 0.3 M, 0.5 M, and 0.7 M. After that, the type of solvent varies, 3% (v/v) hydrogen peroxide, 0.3 M hydrochloric acid, and 0.3 M citric acid. AOS are measured by measuring the amount of reducing sugar using the DNS colorimetric method. The results were then submitted to a one-way ANOVA test followed by a post hoc Tukey Test (P < 0,05). The result of the Tukey test shows that the 0.3 M citric acid treatment significantly has the highest reducing sugar compared to the other solvents. The 0.3 M citric acid solvent also has the highest AOS yield compared to the other solvents. [L1]Can be used as…, but….","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126076494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Orchid Resistance from Ceratorhiza sp. Induction Against ORSV Infection Based on Root Anatomy Character 基于根解剖特征的Ceratorhiza sp.诱导兰花抗ORSV感染的研究

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-12-31 DOI: 10.47007/ijobb.v5i3.82

K. Lestari, S. Wahyuningsih, Mahfut Mahfut, T. T. Handayani

{"title":"Study of Orchid Resistance from Ceratorhiza sp. Induction Against ORSV Infection Based on Root Anatomy Character","authors":"K. Lestari, S. Wahyuningsih, Mahfut Mahfut, T. T. Handayani","doi":"10.47007/ijobb.v5i3.82","DOIUrl":"https://doi.org/10.47007/ijobb.v5i3.82","url":null,"abstract":"Indonesia is the country that has the largest wealth of orchid germ plasma in the world. Some popular types of orchids are Phalaenopsis amabilis and Dendrobium discolor. However, efforts to plant orchids are often hampered by viral attacks, one of which is the Odontoglossum ringpot virus (ORSV). Effective virus control on orchids can be done by using mycorrhiza, one of which is Ceratorhiza. This study aimed to determine differences in the anatomical character of the roots of Phalaenopsis amabilis and Dendrobium discolor, and efficacy of Ceratorhiza sp. in encouraging orchids to overcome ORSV infection. The design used in this study was a factorial randomized design (CRD) with 6 treatments and 4 iterations. Root anatomical observations were performed by making root cross -sections stained with phloroglucin (for lignin observation) and methylene blue staining (for peloton treatment), then observed using a microscope. The results showed that Ceratorhiza sp. in Phalaenopsis amabilis and Dendrobium discolor, the thickness of lignin in the epidermis and carrier bundles was thicker than that of treatment given only for viruses and mycorrhizal viruses.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129178128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Various Doses of Kenikir Flower Crown Extract (Targetes erecta L.) on Reducing Blood Glucose Levels in Rats 不同剂量花冠提取物对大鼠降血糖的影响

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-12-31 DOI: 10.47007/ijobb.v5i3.117

Windi Yusika Kristanti, M. K. Budiyanto, Fendy Hardian Permana

{"title":"Effect of Various Doses of Kenikir Flower Crown Extract (Targetes erecta L.) on Reducing Blood Glucose Levels in Rats","authors":"Windi Yusika Kristanti, M. K. Budiyanto, Fendy Hardian Permana","doi":"10.47007/ijobb.v5i3.117","DOIUrl":"https://doi.org/10.47007/ijobb.v5i3.117","url":null,"abstract":"Diabetes mellitus is a metabolic disease caused by the body experiencing disturbances in controlling sugar levels, and the insulin hormone is impaired or does not meet the requirements. The purpose of this study was to analyze the effect of various concentrations of extracts of the crown of kenikir flower (Tagetes erecta L.) maceration method on decreasing blood glucose levels in white rats (Rattus norvegicus). The research approach used is a quantitative approach. Type of Research True Experimental Research, with the design of The Pre Test Post Test Control Group Design. Provision of kenikir flower crown extract (Tagetes erecta L.) using the maceration method with the help of n-hexane as solvent. This study used 5 treatment groups, namely a negative control group (alloxan), a positive control group (glibenclamide), an extract dose of 25 mg/kgBW, an extract dose of 50 mg/kgBW, and an extract 70 mg/kgBW. Blood glucose levels were measured on day 1 (three days after induction) and day 24 (after treatment). The conclusion of this study was that a dose of 25 mg/kgBW gave the greatest decrease in blood glucose levels with a percentage value of 70% compared to the treatment group with a dose of kenikir flower crown extract.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"109 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114048053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Coffea arabica and Coffea canephora Consumption on Mus musculus Gaster Histopathological Description 食用阿拉比卡咖啡和咖啡对小家鼠胃组织病理学的影响

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-08-31 DOI: 10.47007/IJOBB.V5I2.79

M. Lumaksono, P. Kusumo, F. Gultom

{"title":"Effect of Coffea arabica and Coffea canephora Consumption on Mus musculus Gaster Histopathological Description","authors":"M. Lumaksono, P. Kusumo, F. Gultom","doi":"10.47007/IJOBB.V5I2.79","DOIUrl":"https://doi.org/10.47007/IJOBB.V5I2.79","url":null,"abstract":"Green coffee bean is the bean from coffee fruit that have not undergone roasting process. The purpose of this research was to determine the gastroprotector effects of arabica green coffee bean (Coffea arabica) and robusta green coffee bean (Coffea canephora) to the histopathology (mucosal layer erosion) of mice’s gastric (Mus musculus). This research used a laboratory experimental design with post-test control group design method. Thirty mices divided into three groups. Mice in the control group were only given standard feed and mineral water. Mice in the treatment group I was given 1,8 ml green bean Coffea arabica solution 5,5%. Mice in the treatment group II was given 1,8 ml green bean Coffea canephora solution 5,5%. Both treatments were carried out for seven days, at the same time. Mice’s gastric made into histopathology slides, and then stained with Hematoxylin Eosin. Overview of the histopathology of the gastric assessed using the Wattimena scoring. The results of the assessment are tested by using the statistical test of Kruskall-Wallis, found the significance value of >0,05, which means there is no effect of green bean Coffea arabica and green bean Coffea canephora against the picture of the histopathological mice’s gastric (Mus musculus), as well as no significant difference between the giving of green bean Coffea arabica and green bean Coffea canephora against the picture of the histopathological mice’s gastric (Mus musculus).","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121735913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Methicilin-Sensitive Staphylococcus aureus (MSSA) and Methicilin-Resistant Staphylococcus aureus (MRSA) against Fluoroquinolone and Vancomycin Group Antibiotics in Jakarta 雅加达对氟喹诺酮类和万古霉素类抗生素的甲氧西林敏感金黄色葡萄球菌(MSSA)和耐甲氧西林金黄色葡萄球菌(MRSA)描述

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-08-31 DOI: 10.47007/IJOBB.V5I2.78

C. R. Tjampakasari, Nadira Afida Kalisya, T. M. Sudiro

{"title":"Description of Methicilin-Sensitive Staphylococcus aureus (MSSA) and Methicilin-Resistant Staphylococcus aureus (MRSA) against Fluoroquinolone and Vancomycin Group Antibiotics in Jakarta","authors":"C. R. Tjampakasari, Nadira Afida Kalisya, T. M. Sudiro","doi":"10.47007/IJOBB.V5I2.78","DOIUrl":"https://doi.org/10.47007/IJOBB.V5I2.78","url":null,"abstract":"MRSA is Staphylococcus aureus bacteria that is resistant to methicillin and certain antibiotics. These organisms live as commensal bacteria that are transmitted in communities and health facilities which cause various health problems. Patients who develop antibiotic resistance have a worse clinical condition and many lead to death. The purpose of this study was to compare MSSA and MRSA against fluoroquinolone group and vancomycin antibiotics. Specimens derived from various clinical samples sent to microbiology laboratories FMUI for the period 2018-2019. Gram staining and culture is carried out according to the procedure of each colony that grows on the medium. Followed by identification using the automatic machine Vitek 2®. A total 62 isolates, 58 were MSSA and the others were MRSA (6.45%). MSSA patients are in the age range 1 - >50 years, dominant in men while MRSA is in the age range 16 – 30 and >50 years, dominant in women. The sensitivity of the fluoroquinolone group and vancomycin of antibiotics to MSSA was 100% whereas for MRSA it was 50 % to fluoroquinolone group and 100% to vancomycin. Vancomycin can be the antibiotic of choice for the management of infections by MRSA bacteria.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133290495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antibacterial Effectiveness of Gading Kuning Coconut Extract (Cocos nucifera var. Eburnea) in Aeromonas hydrophila Bacteria In Vitro 加丁昆宁椰子提取物对嗜水气单胞菌的体外抑菌作用

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-08-31 DOI: 10.47007/IJOBB.V5I2.81

Zakiyatul Fachiroh, Irul Hidayati, I. A. Jariyah

{"title":"Antibacterial Effectiveness of Gading Kuning Coconut Extract (Cocos nucifera var. Eburnea) in Aeromonas hydrophila Bacteria In Vitro","authors":"Zakiyatul Fachiroh, Irul Hidayati, I. A. Jariyah","doi":"10.47007/IJOBB.V5I2.81","DOIUrl":"https://doi.org/10.47007/IJOBB.V5I2.81","url":null,"abstract":"Fish disease caused by Aeromonas hydrophila bacteria is very dangerous. These bacteria spread very quickly and result in a decrease in growth rates and can kill fish. To overcome this problem, fish cultivators usually use chemical drugs or antibiotics. However, the use of antibiotics can cause bacterial resistance and the presence of antibiotic residues in fish which can be harmful if the fish is consumed, so it is necessary to do other alternatives using traditional medicine. Yellow ivory coconut husk (Cocos nucifera var. Eburnea) is believed to have antibacterial properties. However, the content of potential secondary metabolites in these plants is not known, therefore this study aimed to determine the ability of the extract of the yellow ivory coconut husk (Cocos nucifera var. Eburnea) in inhibiting the growth of Aeromonas hydrophila bacteria. This study used a completely randomized design (CRD) with 6 treatments and 4 repetitions. The method used in this study is the disc diffusion method. The results of the disc diffusion method showed that the extract of ivory yellow coconut coir (Cocos nucifera var. Eburnea) was able to inhibit the growth of Aeromonas hydrophila bacteria. The concentration of coco coir extract of yellow ivory (Cocos nucifera var. Eburnea) 5000 ppm had the highest inhibition zone value with an average of 12.225 mm. Statistical test results show the value of Asymp. Sig of 0.004 which means <0.05. This indicates that the extract of the yellow ivory coconut husk (Cocos nucifera var. Eburnea) is able to inhibit the growth of Aeromonas hydrophila bacteria","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130726862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Tamarillo (Solanum betaceum Cav.) Fruits Extract Towards Growth of Trichophyton rubrum: in vitro Study 柽柳(茄)的有效性果实提取物对红毛藓生长的体外研究

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-08-31 DOI: 10.47007/IJOBB.V5I2.74

Dandi Tri Dirgantara, Y. Setyaningsih, M. Bahar

{"title":"Effectiveness of Tamarillo (Solanum betaceum Cav.) Fruits Extract Towards Growth of Trichophyton rubrum: in vitro Study","authors":"Dandi Tri Dirgantara, Y. Setyaningsih, M. Bahar","doi":"10.47007/IJOBB.V5I2.74","DOIUrl":"https://doi.org/10.47007/IJOBB.V5I2.74","url":null,"abstract":"Trichophyton rubrum is the most common cause of dermatophytosis. The use of synthetic-antifungals has several side effects and resistance. Exract of tamarillo (Solanum betaceum Cav.) fruits contains active compounds that have potential as antifungal, including alkaloids, saponins, tannins, phenolics, and flavonoids This study aims to determine the effectiveness of tamarillo extract as antifungals against the growth of T. rubrum. This study used ethanol extract of tamarillo fruits with a concentration based on volume/volume (v/v) percentage of 20%; 40%; 60%; and 80%, ketoconazole as positive control, and aquadest as negative control. The antifungal test was conducted by using well diffusion method on Saboraud-Dextrose-Agar medium and was incubated for 24 hours, 48 hours and 72 hours. Data were analyzed by Kruskall-Wallis-test with the results p=0.01, p=0.000, p=0.000 respectively according to the length of incubation time. Data were analyzed for Post-Hoc by Mann-Whitney-test showing the three groups of data had significant differences in results between the two treatment groups. Most effective extract group was the extract with concentration of 20% at incubation time of 24 hours with relatively strong antifungal properties. The results showed that the tamarillo fruits extract as antifungal are effective towards growth of T. rubrum which indicated by the presence of inhibition zone at a concentration of 20%; 40%; 60%; and 80 after being incubated for 24 hours with diameters of 13,375 mm, 15,725 mm, 17,025 mm and 19.25 mm, respectively.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"183 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116503319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of "Soil Transmitted Helminth" Contamination on The Raw Vegetables in Warung Pecel Lele in Kebon Jeruk District, Jakarta 雅加达Kebon Jeruk区Warung Pecel Lele生蔬菜“土壤传播蠕虫”污染鉴定

Indonesian Journal of Biotechnology and Biodiversity Pub Date : 2021-04-30 DOI: 10.47007/IJOBB.V5I1.64

A. Fane, E. Majawati, Harro Harris Liman

{"title":"Identification of \"Soil Transmitted Helminth\" Contamination on The Raw Vegetables in Warung Pecel Lele in Kebon Jeruk District, Jakarta","authors":"A. Fane, E. Majawati, Harro Harris Liman","doi":"10.47007/IJOBB.V5I1.64","DOIUrl":"https://doi.org/10.47007/IJOBB.V5I1.64","url":null,"abstract":"The prevalence of intestinal helminth infections in several provinces in Indonesia including Jakarta reaches 80% (or is still high). Helminth transmission may occur via food/drinks or through the skin. In particular, consumption of raw vegetables allows for the transmission of helminth eggs to happen. The purpose of this study was to identify intestinal worm eggs in raw vegetables sold in warung pecel lele. Raw vegetables were usually served together with the pecel lele menu which is a very popular dish among the Jakartans. The present study is a descriptive study with a cross sectional approach. Seventy-five samples of raw vegetables were included in this study, which were obtained from 25 warung pecel lele in Kebon Jeruk District, Jakarta. Samples were examined for helminth egg contamination based on a sedimentation method by lugol staining. Of all the raw vegetables samples, 20 samples (26.7%) was contaminated by soil transmitted helminths eggs. Ascaris lumbricoides worm eggs were identified in 17 samples (22.7%), whereas hookworm eggs were found in 3 samples (4%). The study suggests that the number of helminth egg contamination identified in raw vegetables are still high among warung pecel lele in West Jakarta.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126133388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0