Indonesian Journal of Biotechnology and Biodiversity最新文献

{"title":"Fungal Diversity and Yeast Patterns of Yeast Sensitifity to Several Antifungals in Jakarta","authors":"C. R. Tjampakasari, Yeva Rosana, Mardiastuti Hw","doi":"10.47007/ijobb.v6i2.131","DOIUrl":"https://doi.org/10.47007/ijobb.v6i2.131","url":null,"abstract":"Fungal infections are common in the tropics. The cause of mycoses depends on the cause and the accompanying risk factors. It is important to do culture and identification to determine the etiology and continue with antifungal sensitivity examination to determine the appropriate management. The method of treating a yeast infection depends on the type of infection, its severity and the part of the body that is infected. As various fungi cause infections, the antifungal drugs used are also very diverse. The study was conducted during 2020 consisting of  350 samples of various specimens with positive cultures of 116 (33.14%). The results of identification using Vitek 2®   automated system and slide culture obtained 19 species on fungus, consistinf of 99.14% yeast and  0.87% mold. The most yeast found were C. albicans 26.27%  and Aspergillus sp;  Rhizopus  0.86 %  respectively from mold. The pattern of sensitivity of yeast to several antifungals showed that Fluconazole was effective at 96.43%, followed Voriconazole and Amphothericin B 93.24% respectively. The next are Flucytocin, Micafungin and Caspofungin 91.66%,  86.18% and 85.74% respectively. Culture, identification and sensitivity examination need to be improved to obtain images and information that reflects.Keywords : yeast, mold, sensitivity patterns, antifungal  ","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129116343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATING THE ANTIOXIDANT ACTIVITY OF Echinacea purpurea (L.) Moench ETHANOL EXTRACT","authors":"P. F. Arifin, Theoprawira Imanuel Yobert Assa, Adri Nora, Rosalina Wisastra","doi":"10.47007/ijobb.v6i2.132","DOIUrl":"https://doi.org/10.47007/ijobb.v6i2.132","url":null,"abstract":"The purpose of this research was to extract the yield of Echinacea purpurea at the SOHO Center of Excellence in Herbal Research Sukabumi on a laboratory scale and determine the antioxidant effect. This plant is known to grow in Europe before being cultivated in Sukabumi, Indonesia. Additionally, the specimen contains compounds with antioxidant functionalities, estimated to be useful in cell protection from oxidative stress. This property is attributed to the presence of alkamide compounds. In this research, Simplicia was extracted using 2 different methods, and those generated by DPPH yielded the best chicoric acid value and were further evaluated for antioxidant activities. The results showed the best results in samples isolated with maceration at 70℃ temperature, stirred for 1.5 hours, compared to those heated at 55℃. Consequently, the yield obtained using this technique was 23.24 grams which had 4.36% chicoric acid content and total phenol of 13.82%. The thick Echinacea purpurea extract demonstrated the best IC50 value of 92.08 µg/mL, which was better than the other samples. This research suggests a need to improve the extraction process as an attempt to achieve optimal results.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132676588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pretreatment and Composition of Trichoderma Viride and Zymomonas Mobilis Consortium on Bioethanol Production from Leaf Litter","authors":"Wulan Fitriani Safari, Mohamad Syafaat","doi":"10.47007/ijobb.v6i2.134","DOIUrl":"https://doi.org/10.47007/ijobb.v6i2.134","url":null,"abstract":"Bioethanol is an alternative biofuel that is widely produced in bulk to replace and complement petroleum-based fuels. Bioethanol is produced through the alcoholic fermentation of sucrose or simple sugars. One of the potential source for bioethanol production that has not been studied is leaf litter. Various studies have shown that T. viride and Z. mobilis can be used for bioethanol production, but there has been no report on the production of bioethanol from leaf litter using a consortium of T. viride and Z. mobilis using the SSF method. This study aims to examine the effect of pretreatment and composition of the consortium of T. viride and Z. mobilis on the production of bioethanol from leaf litter. Leaf litter was dried and mashed, then pretreatment was carried out with 32% NH3 solution. The bioethanol production process using the SSF method uses a consortium of T. viride and Z. mobilis with a composition of 5%: 5%, 10%: 5%, 5%: 10% and 10%: 10% (w/v). SSF was carried out for 72 hours at a temperature of 35oC and pH 5. The bioethanol was purified by distillation and the ethanol content was tested with GCMS. Higher levels of bioethanol were found in leaf litter with pretreatment than without pretreatment. The highest ethanol content (0.6721%) was obtained from SSF with consortium composition of T. viride : Z. mobilis (10% : 10%) from leaf litter with pretreatment. Pretreatment and composition of the consortium T. viride and Z. mobilis affect the production of bioethanol from leaf litter.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123366281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR CHARACTERIZATION OF HAP3 GENE IN CASSAVA (Manihot esculenta Crantz)","authors":"M. Su’udi, A. N. Puspito, S. Arimurti, Lailiyah Maulidatul Hasanah, Asyifa Yasmin Arum","doi":"10.47007/ijobb.v6i2.136","DOIUrl":"https://doi.org/10.47007/ijobb.v6i2.136","url":null,"abstract":"Cassava (Manihot esculenta Crantz) is one of the food crops that has higher resistance to dry land, so it is possible to grow it in dry land. Cassava is used as an alternative food crop to maintain world food security, because the tuber has a high carbohydrate value, is easy to plant and is able to support food diversification. Cassava in Indonesia already has several nationally superior varieties such as Adira 1 and Malang 6. The first stage of cassava breeding is knowing the characteristics of genes in these plants, one of which is HAP3. The purpose of this study was to characterize the HAP3 gene in cassava (Manihot esculenta Crantz) using a molecular approach with genomics and bioinformatics. The methods used were DNA isolation of cassava leaves of Adira 1 and Malang 6 varieties, amplification by PCR, analysis of PCR results, and data sequencing. The results showed that the HAP3 gene in cassava varieties Malang 6 and Adira 1 had 100% homology with cultivar AM560-2 in nucleotide and amino acid sequences. HAP3 cassava varieties Malang 6 and Adira 1 have the closest relationship with Hevea brasiliensis plants with a homology value of 90.91% and Ricinus communis 83.33% based on amino acid sequences. The size of HAP3 on cassava varieties Adira 1 and Malang 6 was 546 bp.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129243022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of Cattleya sp. Orchid Planlet In vitro Selection Results Against Drought Stress with Polyethyleneglycol (PEG) 6000","authors":"Aura Priscilla Sabatini, E. Nurcahyani, Yulianty Yulianty, R. Agustrina","doi":"10.47007/ijobb.v6i2.129","DOIUrl":"https://doi.org/10.47007/ijobb.v6i2.129","url":null,"abstract":"Orchid Cattleya sp. is famous for ornamental plants, known as the queen of flower. The water content is very much needed, so that drought conditions do not become a barrier in its cultivation. Cattleya sp. plantlets can be resistant to drought stress through in vitro culture selection with PEG 6000. The purpose of this study was to determine the proportion of live plantlets that were tolerant to drought stress and to analyze the of the plantlet Cattleya sp. that experienced drought stress after being selected with PEG 6000 included: visualization of plantlets, chlorophyll a, b, and total content and stomata index. This research was conducted in a completely randomized design consisting of 1 factor, namely PEG 6000 with 5 levels: 0%, 5%, 10%, 15% and 20% with 5 repetitions. The homogeneity of variance was tested using Levene's test at a 5% significance level, followed by One-Way ANOVA at a 5% significance level, then if the data showed a significant difference, it was followed by a Significant Difference Test at a 5% significance level. The results of this study showed that the proportion of live plantlets was 60% at a concentration of 20% PEG 6000. The Cattleya sp. plantlets a, b, total chlorophyll’s and the stomatal indekx all decrease in response to the increasing PEG 6000 concentration.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128770791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germination Process of Bud Chips of 3 Commercial Sugarcane (Saccharum officinarum L) Varieties at PT. Gunung Madu Plantations","authors":"Syarifah Nuraini, Mahfut Mahfut, Rifki Bangsawan","doi":"10.47007/ijobb.v6i1.124","DOIUrl":"https://doi.org/10.47007/ijobb.v6i1.124","url":null,"abstract":"The increase in demand for sugarcane (Saccharum officinarum L) as an industrial raw material is not accompanied by an increase in national sugarcane production. The decline in sugarcane production was due to errors in cultivation. In the growth phase, germination is the most critical and vulnerable phase, resulting in sugarcane cultivation and subsequent sugarcane growth. The purpose of this study was to determine the germination process of bud chips of 3 commercial sugarcane varieties. The experiment was carried out in March-May 2021 at the Research and Development experimental garden of PT. GMP uses a Randomized Block Design. There were 3 varieties observed, namely GMP3, GMP5, and GMP7. Each Bud chip was planted using an individual technique with a spacing of 50 cm and covered with soil. Observations were made until the 30th day after planting. The results showed that the GMP7 and GMP3 varieties included varieties that had the most stable type of germination and relatively fast organ growth stages, while the GMP5 variety had a relatively slow germination type for each organ formation and plant growth.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128555310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of Polymerase-Producing Thermophilic Bacteria from Ijen Crater, East Java","authors":"Hidayah Murtiyaningsih, L. Arum, Rendi Anggriawan","doi":"10.47007/ijobb.v6i1.123","DOIUrl":"https://doi.org/10.47007/ijobb.v6i1.123","url":null,"abstract":"Thermophilic bacteria are a group of bacteria that adapt to environmental conditions with high temperatures, ranging from 45 ° - 90 ° C. as the ring of fire, Indonesia has many volcanic areas as the source of thermophilic bacteria, once is in Ijen Crater, East Java. Thermophilic bacteria have the potential to produce heat-resistant / thermostable enzymes such as polymerase. DNA polymerase is a key enzyme in amplification process of DNA fragment. The objective of this study was to found the novels thermophilic bacteria isolated from Ijen Crater which has potential to be the source of DNA polymerase production. This study was carried out by growing the bacterial strains on suitable media, followed with thermal screening (70-900C), isolating and identifying the morphological and molecular characteristics of the selected isolates. Total of 12 isolates have selected after thermal screening in 700C for, which were identified as positive and negative bacteria in gram staining assay. All the selected isolates found to have same colony color in white, but various types in shape, border, elevation, and border with. There were 4 isolates that selected after incubation in 900C for 30 minutes, which were isolate 1, 5, 9, and 11. The DNA genome of four selected isolates then amplified using universal primer (16S rDNA) and pol1 to detect the presence of gene encoding polymerase. The result indicated that isolate 1, 5, 9, 11 were able to be the candidate thermophilic bacteria to produce DNA polymerase.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123037665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Indole Acetic Acid (IAA) by Serratia marcescens strain MBC1 on Soybean (Glycine max L.) Germination","authors":"Ayu Ismawanti, E. Nurcahyani, Salman Farizi, S. Sumardi","doi":"10.47007/ijobb.v6i1.118","DOIUrl":"https://doi.org/10.47007/ijobb.v6i1.118","url":null,"abstract":"Indole Acetic Acid (IAA) is one type of hormone that can stimulate plant growth. IAA hormone is able to accelerate plant growth, especially in the early stages of growth. IAA hormones are not always found in plants, one of which can be found in microorganisms. Rhizobacteria group have the ability to produce IAA hormones. This study aims to determine whether the IAA produced by Serratia marcescens strain MBC1 is able to accelerate the germination process of soybean seeds (Glycine max L.). The results of the Analysis of Variance (ANOVA) giving the production of IAA Serratia marcescens strain MBC1 of 8.31 ppm and 9.35 ppm to soybean seed germination significantly increased root length and stem length. The increase in germination from the administration of IAA Serratia marcescens MBC1 was still smaller than the positive control using synthetic IAA with a concentration of 8 ppm which was able to significantly increase soybean germination.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116837294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profile and Antioxidant Capacity of Phenolic Compounds from Rice Straw","authors":"Chessa Uly Thalia, Clairine Nathania, J. Sukweenadhi, M. Purwanto","doi":"10.47007/ijobb.v6i1.125","DOIUrl":"https://doi.org/10.47007/ijobb.v6i1.125","url":null,"abstract":"ABSTRACTA study was conducted with the purpose of comparing the antioxidant properties of the aquadest extracts of three varieties of red rice straw: Memberamo, Ciherang, and Barak Cenana. The extraction was done using ultrasound-assisted extraction (UAE) technique. The compound profiling of the phenolic compounds in the extracts were done by thin-layer chromatography (TLC) and gas chromatography (GC). The antioxidant activity measurements were done using the DPPH and FRAP assays, using garlic acid as the standard. The total phenolic content assay showed no significant difference in the total phenolic content among the three rice straw varieties. TLC results showed two presumable flavonoids in Memberamo and Ciherang rice straw extracts, and three presumable flavonoids in Barak Cenana rice straw extract. GC analysis results detected three components in Memberamo rice straw extract, five components in Ciherang rice straw extract, and six components in Barak Cenana rice straw extract. The DPPH assay results showed that Barak Cenana rice straw extract indicated the highest antioxidant activity among the three varieties, while the FRAP assay results showed no significant difference in the antioxidant activity among the three rice straw varieties. Keywords: Antioxidant, phenolic compounds, rice straw, ultrasound-assisted extraction ABSTRAKPenelitian dilakukan untuk membandingkan sifat antioksidan dari ekstrak akuades jerami tiga varietas padi, yaitu padi Memberamo, padi Ciherang, dan padi Barak Cenana. Ekstraksi dilakukan dengan metode ultrasound-assisted extraction (UAE). Penelitian profil senyawa fenolik dalam ekstrak dilakukan dengan metode kromatografi lapis tipis (KLT) dan kromatografi gas (GC). Pengukuran aktivitas antioksidan dilakukan dengan uji DPPH dan uji FRAP menggunakan asam galat sebagai standar. Pengukuran kadar fenolik total menunjukkan bahwa tidak ada perbedaan signifikan antara kadar senyawa fenolik ekstrak jerami ketiga varietas padi. Hasil KLT menunjukkan dua senyawa yang diduga golongan flavonoid pada ekstrak jerami varietas Memberamo dan Ciherang, serta tiga senyawa yang diduga golongan flavonoid pada ekstrak jerami varietas Barak Cenana. Analisis GC mendeteksi tiga komponen dalam ekstrak jerami padi Memberamo, lima komponen dalam ekstrak jerami padi Ciherang, serta enam komponen dalam ekstrak jerami padi Barak Cenana. Uji DPPH menunjukkan bahwa aktivitas antioksidan tertinggi dimiliki oleh jerami padi Barak Cenana, sedangkan uji FRAP menunjukkan bahwa tidak ada perbedaan siginifikan antara aktivitas antioksidan ekstrak jerami ketiga varietas padi. Keywords: Antioksidan, senyawa fenolik, jerami padi, ultrasound-assisted extraction  ","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126432871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Inhibition of Twenty Natural Compound From Marine Algae Against Main Protease (Mpro) Sars-CoV-2 and Human Maltase-Glucoamylase : A Preliminary In Silico Study","authors":"R. Astuti, Angga Wira Perdana, B. Kusuma, H. S. Yufidasari, A. Bhawiyuga, A. A. Hermosaningtyas","doi":"10.47007/ijobb.v6i1.122","DOIUrl":"https://doi.org/10.47007/ijobb.v6i1.122","url":null,"abstract":"For over two years, the world has been battling the devastating Coronavirus Disease-2019 (COVID-19), a highly infectious disease caused by SARS-CoV-2 virus. Even though vaccination has shown rapid progress, another way of treatment and prevention is still very much needed, considering the massive spread of the virus. The severity of the symptoms, the time onset of the symptoms to death and death rate may vary, affected by the patient’s age, immune condition and comorbidities, such as hypertension and diabetes. This study was designed to identify the potential binding activity of twenty compounds from marine algae against SARS-CoV-2 main protease and human maltase-glucoamylase, a receptor related to diabetes, using in silico approach. Out of twenty compounds, kappa carrageenan, β-carotene, and astaxanthin showed potential activity against the main protease employing the blind docking method. The top three compounds were subjected to docking against the active site of the main protease only, resulting in astaxanthin (-8.3 kcal/mol) followed by kappa-carrageenan (-8 kcal/mol) as the most potential ligand. On the other hand, quercetin (-9.1 kcal/mol) and kappa-carrageenan (-8.9 kcal/mol) showed the best docking score against human maltase-glucoamylase. The binding mode of each potential compound was visualized using the Discovery Studio Visualizer software. This study provides the potential compound as per the docking method. Further analysis such as dynamics simulation, in vitro and in vivo studies are needed to examine the potential use of these compounds as COVID-19 treatment and or supplement within COVID-19 pandemic, especially for people with diabetes.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117118848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}