Luluk Muzayyana, M. Hazmi, Hidayah Murtiyaningsih, L. Arum
{"title":"Optimization of Honey Concentration on In Vitro Sorghum (Sorghum bicolor ) Shoot Induction","authors":"Luluk Muzayyana, M. Hazmi, Hidayah Murtiyaningsih, L. Arum","doi":"10.47007/IJOBB.V4I2.68","DOIUrl":"https://doi.org/10.47007/IJOBB.V4I2.68","url":null,"abstract":"Sorghum can be developed a functional food, feeding and bioethanol. The genetically modified development is threated regeneration in vitro. The purpose study to get an optimum concentration of honey on MS media for the growth of sorghum shoots. The study was carried out using a randomized complete design with a single treatment of honey concentration, 4 replications. The concentration of honey used covers 0, 5, 10, 15 and 20 g/l. Observation parameters consist of time shoot appears, percentages emerge shoots, high shoots and number of leaves. Data obtained were analyzed by F test further more with DMRT 5%. Study results showed that there is no significantly influenced on time shoot appears, high shoot 9, 12, and 15 hsi, while the real difference in the number of shoots, percentage emerge shoot and high shoot 6 hsi. The treatment 5 g/l of honey able to accelerate time shoot appearance, and honey treatment 10 g/l result in the percentage of the highest shoot of 72%, the number of shoots 0,72 and the highest growth length 0f 5,02 mm at 6 hsi, was the best treatment.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123540274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histology Analysis of Tissue Regeneration Process of Digit Tip Mice (Mus musculus) post amputation","authors":"T. Novianti, F. D. Wahyuni, I. Jamilah, S. Ilyas","doi":"10.47007/IJOBB.V4I2.70","DOIUrl":"https://doi.org/10.47007/IJOBB.V4I2.70","url":null,"abstract":"The ability to regenerate tissue is different for each organism. Mice (Mus musculus) able to regenerate the 3rd phalange of a digit. The tissue regeneration process has four phases are the wound-healing phase, the blastema phase, the regeneration phase, and the maturation phase. Each phase has a different process and different activity of cells. Histological analysis is very important to see the activity of each cell in each phase of tissue regeneration. Through histological analysis we can find out the role of each cell in the tissue regeneration process as well as the processes that occur in tissue regeneration. In this study, we analyzed tissue histology in the digit tip mice at each regeneration phase post amputated. The phalanges were amputated on the 3rd phalanges of digit tip of 24 male mice which had been previously sedated using ketamine / xylazine. Digit tip were allowed to grow and regenerate, and samples were taken on days 0, 1, 3, 5, 10, 15, 25 after amputation. Histological analysis was performed using Hematoxylin-eosin staining on a sample preparation that had been made into paraffin blocks first. The histological showed that at the beginning of the wound, the tissue rapidly forms a thin epidermal layer to cover the wound. In the wound healing phase, some of embryonal cells proliferated and migrated actively. In the blastema phase, granule cells cluster to form various new tissues. In the regeneration phase, new tissue begins to form, such as blood vessel, muscle, bone, and epidermal tissue. In the regeneration phase on day 15, several new tissues have begun to form, such as blood vessel tissue, muscle, hemorrhoid, bone and epidermis. Finally, in the maturation phase on day 25, the tissue morphology process occurs and perfecting the digit tip mice tissue. ","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"238 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116455323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Utilization of Tree Canopies by Avifauna in Two Types of Habitat in Telaga Warna Nature Tourism Park, Bogor, West Java","authors":"Fauzan Cholifatullah, Annisa Ramadani, Annisa Haryanti Nurhasanah, Ayu Tantri Winna Asmara, T. Setia","doi":"10.47007/IJOBB.V4I2.60","DOIUrl":"https://doi.org/10.47007/IJOBB.V4I2.60","url":null,"abstract":"Trees have a variety of canopy forms that are used by birds to rest, eat and socialize. Tree canopies are divided into two categories based on their position, namely vertical and horizontal. Telaga Warna Nature Tourism Park (TWA) is a public space that is considered to host a diversity of birds and vegetation. The area has two different types of habitats, namely ecotone, and primary forest. This study aimed to determine diversity of bird species that make use of tree canopy in the two types of habitat. The study was conducted in July, September, and December 2018. Bird data collection in the form of tree canopy utilization (vertically and horizontally) was conducted at the time of 07.00 to 12.00 and 13.00 to 17.00, using visual encounter survey method. The results showed that there are 48 species of birds from 22 families used the entire canopy horizontally and vertically. The sequence of level of vertical utilization in both ecotone habitat and primary forest in descending order is found as follows: strata 3 with 30 species, strata 4 with 18 species, strata 1 with 17 species, strata 2 with 13 species, and strata 0 with 9 species. The level of horizontal canopy utilization by birds in both ecotone habitats and primary forests is found to be higher for edge canopies when compared to inner canopies, with 31 species recorded for edge canopies and 27 species recorded for inner canopies.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"161 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116636933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Sensitivity Enterobacteriaceae of Extended Spectrum BetaLactamase (ESBL) against Antibiotics of Quinolone and Carbapenem Group in Clinical Microbiology Laboratory, Faculty of Medicine, Universitas Indonesia","authors":"C. R. Tjampakasari, Alya Iranti, T. M. Sudiro","doi":"10.47007/IJOBB.V4I2.63","DOIUrl":"https://doi.org/10.47007/IJOBB.V4I2.63","url":null,"abstract":"Antibiotic resistance is a challenge in medical problems. One prevalence of resistance that tends to expand globally is against ESBL-producing Enterobacteriaceae, a group of bacteria capable of destroying beta-lactam antibiotics. The known ESBL producing bacteria are E. coli and K. pneumoniae. This study aims to compare the sensitivity of quinolone and carbapenem antibiotics to ESBL-producing bacteria based on data obtained from Clinical Microbiology Laboratory, Faculty of Medicine, Universitas Indonesia through 2018-2019. Using the Vitek 2® Compact identification method, the results showed that the prevalence of E. coli and K. pneumoniae ESBL was positive less than 5%. All of the ESBL–producing E. coli came from urine specimens, while ESBLproducing K. pneumoniae came from different types of specimens which are sputum and blood. Most prevalence comes in the age range >50 years with female gender. In general, antibiotic sensitivity to the quinolones was less than 50% against ESBL-producing E. coli. Meanwhile, the sensitivity of carbapenem antibiotics reached 100% both against ESBL-producing E.coli and K.pneumoniae.","PeriodicalId":236342,"journal":{"name":"Indonesian Journal of Biotechnology and Biodiversity","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125823614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}