Vestnik of Ulyanovsk state agricultural academy最新文献

{"title":"IMPROVEMENT OF THE METHOD OF OBTAINING WATER-SOLUBLE PEPTIDES ISOLATED FROM M. DOMESTICA AND STUDY OF THEIR PROPERTIES","authors":"O. Larionova, L. S. Krylova, Y. Drevko, K. Smirnova","doi":"10.18286/1816-4501-2022-3-135-141","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-135-141","url":null,"abstract":"The article presents data on improvement of the method of obtaining fractions of water-soluble peptides by cold extraction from the biomass of Musca domestica larvae, their separation using dialysis membranes with sizes less than 3.5 kDa, 3.5-7 kDa, 7-14 kDa, more than 14 kDa, followed by a study by means of highly effective liquid chromatography. Retention time and chromatographic yield, protein content, antimicrobial properties, acute toxicity of 2 (BP2) protein fraction, which is the fraction isolated when using membranes of 3.5 - 7 kDa, were established. Therefore, this fraction has a mass of proteins in the range from 3.5 kDa to 7 kDa. The results of the antimicrobial activity of the analyzed protein fraction 2 to the following strains were obtained: B. cereus 8035, E. aerogenes ATCC-13048, E. coli Mordovia 21. It was found that the protein fraction 2 at a concentration of 10 mg/ml, 5 mg/ml, 2.5 mg /ml had antimicrobial activity against E. aerogenes ATCC-13048, E. coli Mordovia 21. Acute toxicity of this fraction was assessed on laboratory mice after a single intragastric and parenteral administration. The average lethal dose for intragastric and parenteral administration for 20% solution of protein fraction 2 could not be established, since the maximum possible doses for these types of administration did not lead to death of any animal. It was established that 20% solution of protein fraction 2 belongs to the 4th hazard class (substances of low hazard) according to the generally accepted hygienic classification.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85046478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDENTIFICATION OF NEW SNPS IN GENES/LOCI OF RESISTANCE TO FUSARIOSIS AND CHITINASE","authors":"A. Nalbandyan, T. S. Rudenko, A. S. Fomina","doi":"10.18286/1816-4501-2022-3-110-115","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-110-115","url":null,"abstract":"The aim of this work is to test specific primers FusA1F/R, 2Ch300F/R, and 7Ch310F/R to study SE2 gene responsible for expression of acid chitinase in stressful situations, and loci that ensure resistance of sugar beetroot breeding material to Fusarium rot. Material for the study was sugar beetroot plants of domestic and foreign selection. To confirm the connection of SE2 gene (localized on chromosome 3)which controlsstable level of acid chitinase with resistance of sugar beetroot to root rot, ten samples of sugar beetroot were typed using molecular genetic marker FusA1. Two 600 b.p. and 400 b.p. DNA fragments in all studied genotypes, except wild beetroot plants (Beta corolliflora Zoss.)were identified. As a result of the studies of SE2 gene, eight single nucleotide substitutions (3 T/C, 2 C/G, A/G, G/A, C/T) and three single nucleotide insertions (nucleotide A) were identified in plants of Shannon foreign hybrid compared to the resistant genotype. As for loci encoding protective proteins (regions of the second and seventh chromosomes) and involved in formation of resistance to Fusarium rot, SNPs were also found in domestic breeding materials and foreign hybrids. The Geneious Prime tool was used to analyze and align the sequenced sequences.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86325959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARATIVE ANALYSIS OF EGGMORPHOMETRIC PARAMETERS OF HENS OF DIFFERENT GENOTYPES","authors":"A. Dzhagaev, A. Vetokh, E.R. Ilyina","doi":"10.18286/1816-4501-2022-3-175-180","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-175-180","url":null,"abstract":"The paper shows the results of assessing the quality of eggs according to morphometric and morphological parameters of purebred hens of meat and egg productivity, as well as their crossbreeds of the first generation in order to compare them, to identify significant differences in egg quality, with subsequent application of the results for forecasts of hatching house work for obtaining of a model population of chickens and the possibility to search for candidate genes responsible for the commercial qualities of eggs. Collection of morphological and biophysical parameters was carried out on a highly sensitive analyzer Stable Micro Systems, laboratory electronic scales Ohaus and using caliper. Analysis of eggs obtained from 3 groups of analogue hens, namely Russian white (egg), Cornish white (meat) hens and their F1 offspring at the age of 44-52 weeks, showed their compliance with incubation and commercial quality parametres. Significant differences were established between the experimental groups in terms of shape index and egg weight, shell weight, and dense protein weight. A significant difference was found in regards to shell thickness: crossbred individuals were 10% inferior to their purebred analogues. As far as shell color is concerned, crossbred hens inherited characteristics from both parent breeds. Analysis of liquid components of the egg showed the genotype influence only on yolk index, whereasyolk color and protein quality according to Haugh units were without significant differences. Crossing Cornish meat breed and Russian White egg breed does not adversely affect the quality of the eggs. The obtained result confirms to some extent the splitting of genes when obtaining a model population of chickens by crossing parental individuals of opposite directions of productivity.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79537240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEMATOLOGICAL STATUS OF SIMMENTAL BREDINSKY MEAT TYPE HEIFERS WITH DIFFERENT ALLELIC SET OF CAPN1 AND TG5 GENES","authors":"S. D. Tyulebaev, M. D. Kadysheva","doi":"10.18286/1816-4501-2022-3-186-191","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-186-191","url":null,"abstract":"The work carried out by geneticists in recent years connected with whole genome genotyping of cattle by SNP polymorphism with identification of associations with qualitative and quantitative parameters of productivity is used in innovative breeding programs. However, biochemical mechanisms of such determinations are not fully disclosed. Expression of polymorphic genes, which leads to a change of protein properties, is associated with biochemical processes which occur in the animal body at biomolecular level, which can manifest itself in neurohumoral and metabolic processes, which can possibly be traced by analyzing blood parameters. In this regard, blood was taken from the heifers of Bredinsky meat type of Simmental breed in Chelyabinsk region in order to isolate DNA to determine polymorphism of CAPN1 and TG5 genes associated with qualitative parameters of meat productivity and determine hematological parameters. The analysis of the genotyped population revealed a certain alignment of polymorphic state of the genes of the animals of the studied sample. The distribution of hematological parameters of heifers according to the genotypes of the studied genes did not show a clear significant difference, but outlined some tendencies. According to morphological composition of the blood, these tendencies were less expressed. However, the distribution of blood biochemical parameters by genotypes of CAPN1 gene showed superiority of GG genotype by blood glucose by 0.51 mmol / l, which is 21.9%, the content of ALT transamination enzymes by 11.9%, cholesterol by 18.8% . Difference tendencies were noted for genotypes of TG5 gene by creatinine (12.0%) and amylase (9.5%) content. The results of this study may be useful in terms of accumulating new research data and contribute to a better understanding of molecular mechanisms of relations among blood parameters, genes, and biochemical processes in animals.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74391584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GROWTH FEATURES OF ABERDEEN ANGUS BREED IN DIFFERENT HOUSING TECHNOLOGIES","authors":"N. Konik, E. Gosteva, Zh.T. Alagirova","doi":"10.18286/1816-4501-2022-3-214-219","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-214-219","url":null,"abstract":"The article presents results of the studies of dynamics of live weight, average daily gains and relative growth rate of Aberdeen Angus bulls under different housing technologies. The purpose of the research is to establish the degree of growth parameters for cattle of Aberdeen-Angus breed in economic and technological conditions of the Kabardino-Balkarian Republic. Bulls of Aberdeen-Angus breed were the object of the research. Group I - year-round keeping in a fenced area, group II - stall-pasture with transfer of animals in the summer to high-mountain pastures (the length of the pasture period is 152 days). Total duration of production cycles was 18 months. The suckling period was 7 months, rearing - 9 months and intensive final fattening - 2 months. It was established that (due to differences in housing technologies), differences between individuals of different groups at the age of one year reached 18.1 kg (P> 0.95) in favor of animals located on high mountain pastures with high nutritional value of the grass stand. The superiority of this group of bulls over the peers of year-round keeping in a fenced area at the age of 16 months was 21.5 kg (P> 0.95), at the end of the fattening period -25.1 kg (P> 0.95). Over the entire period of research, as expected, the maximum average daily gain of live weight was common for Aberdeen Angus animals of remote pasture keeping - 965 g versus 919 g - ofanimals of year-round keeping in a fenced area (by 46 g, Р> 0.95) , in terms of growth energy – by 0.9% (Р>0.95). A comparative assessment of housing technologies for bulls of Aberdeen-Angus breed in economic and technological conditions of the Kabardino-Balkarian Republic showed higher growth parameters in the conditions of distant-mountain keeping compared to year-round keeping in a fenced area.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81265997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF ACUTE AND SUBCHRONIC TOXICITY OF PRAVAD FEED ADDITIVE","authors":"E. Romanova, L. Shadyeva, E. Turaeva","doi":"10.18286/1816-4501-2022-3-142-147","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-142-147","url":null,"abstract":"The article presents results of studies of a new highly effective feed additive \"Pravad\" based on probiotics, adaptogenes, vitamins and amino acids, which has shown itself to advantage in fish farming. Acute toxicity of Pravad feed additive was studied on laboratory animals (rats) and the hazard class was established when it was orally administered. Subchronic toxicity of the feed additive was studied, its results allowto identify the presence or absence of a combination of functional and/or morphophysiological disorders of organs and systems in case of its application. The components that make up the new feed additive are, according to State Standard GOST 12.1.007, low-toxic substances of the 4th hazard class. The obtained results on the study of acute and subchronic toxicity of Pravad feed additive showit has no toxic effect. Long-term observations of laboratory animals on which Pravad feed additive was tested did not reveal any signs of somatic distress. The results of assessment of biochemical parameters and leukocyte formula did not reveal significant differences among parametres of the experimental and control groups. This indicates that introduction of Pravad feed additive into the ration does not have a negative impact on homeostasis of animals. Intragastric administration of Pravad feed additive did not cause significant changes in the mass coefficients of kidneys, lungs, spleen, heart and liver compared to control animals. Pathological and anatomical autopsy of the animals of the experimental group did not reveal any deviations of internal organs in comparison with the animals of the control group.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90558348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANALYSIS OF PARAMETERS OF MORPHOFUNCTIONAL STATE OF PIG OOCYTE LIPIDOME IN CASE OF IN VITRO CULTIVATION","authors":"D. Starikova, T. Kuzmina","doi":"10.18286/1816-4501-2022-3-154-159","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-154-159","url":null,"abstract":"Identification of effective markers of oocyte maturation based on morphological and functional characteristics of intracytoplasmic lipid droplets will allow to increaseinformative value of the criteria for assessing the quality of donor gametes for improvement of the efficiency of using cellular reproductive technologies in animal husbandry, biomedicine, and veterinary medicine. The aim of the study is to analyze the parameters of morphofunctional state of the lipidome (the morphology of lipid droplets and the intensity of fluorescence of the Nile red/lipid droplets complex - Nile red IF) in Susscrofadomesticus oocytes before and after in vitro cultivation. Oocytes were matured using Sage Media Cleavage (CooperSurgical, USA) with 5% Serum Protein Substitute serum (CooperSurgical, USA), 10 IU human chorionic gonadotropin (Sigma, USA). As a result of ranking the population of donor oocytes according to morphology of lipid droplets (granules and clusters) and Nile red IF (low, medium, high), it was found that, the proportion of oocytes with an average Nile red IF and lipid droplets (LD) in the form of granules was 63% before cultivation, which was significantly higher than the proportion of gametes with an average IF of Nile red and LD of a mixed form of 41% (P<0.05). After cultivation, the level of oocytes with medium Nile red IF and LD in the form of granules was 45%, which exceeded the level of gametes with the same Nile red IF and mixed LD of 23% (P<0.05). The proportion of in vitro matured oocytes with high Nile red IF and mixed LD morphology (granules and clusters) was 33%, 14% were characterized by the above parameters before cultivation (P<0.05). Comparative morphological analysis of the relation between the parameters of morphofunctional activity (morphology and IF Nile red) of lipidome in Susscrofadomesticus oocytes was carried out for the first time. Features of functional activity of lipidome before and after cultivation were revealed. The usage of a comprehensive analysis of parameters of functional activity of lipidomeforassessment of the quality of the oocyte is substantiated. Pig oocytes which morphology of lipid droplets is represented by a granular form are promising for cultivation, while Nile red IF is assessed as low. With regard to the analysis of the obtained results, it is recommended to use the proposed comprehensive assessment of lipidome based on morphology and Nile red IF to assess the initial population of donor oocytes in cellular reproductive technologies.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"55 31","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91399473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WATER-RETAINING CAPACITY OF PLANTS OF SPRING SOFT WHEAT VARIETIES IN DRY CONDITIONS OF ORENBURG CISURALS","authors":"I. Besaliev, A. Panfilov, N. S. Reger","doi":"10.18286/1816-4501-2022-3-20-25","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-20-25","url":null,"abstract":"The main factors that reduce spring soft wheat yield in the conditions of Orenburg region are high air temperature and lack of moisture in the soil. Water-retaining and water-absorbing capacities of resistant plants increase under the influence of drought. The aim of the research was to study water-retaining capacity of spring soft wheat varieties depending on the methods of the main tillage. The studies were carried out in the central zone of Orenburg region on southern carbonate, medium-thick, heavy loamy black soil. The water-retaining capacity of plants and spikes of spring soft wheat varieties was determined in the phases of booting and heading by the wilting method (according to Arland) in accordance with the guidelines of VIR. It was established that in case of plowing, water-retaining capacity of plants and spikes of the studied soft wheat varieties is higher compared to stubble background. Water-retaining capacity of spring wheat plants increased from the booting phase to the heading phase. The highest water-retaining capacity in the booting phase was noted in Tulaikovskaya Zolotistaya variety, and in the heading phase - Ulyanovskaya 105.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"5120 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86795657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BLOOD BIOCHEMICAL PROFILE OF CALVES SUFFERING FROM BRONCHOPNEUMONIA","authors":"S.V. Shobolev, E. M. Maryin, N. Kalyazina","doi":"10.18286/1816-4501-2022-3-160-163","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-160-163","url":null,"abstract":"Diseases of respiratory tract organs of young farm animals hold a valuable place among all pathologies, and are in second place after gastrointestinal diseases. One of the most common diseases is bronchopneumonia. The purpose of this work was to study the effect of «Immunouracil 2% solution for injection» medication both separately and in combination with \"Amoxicillin - 150\" antibiotic in treatment of calves with bronchopneumonia on protein metabolism. Experimental studies were carried out on the basis of OOO Megafarma Oktyabrskiy, Cherdaklinsky district, Ulyanovsk region, in the winter-spring period of 2022. Three groups of 10 calves in each were formed,t hey were treated according to the research plan. In the control group, the antibiotic Penstrep-400 LA was used, in the first experimental group – the drug \"Immunouracil 2% solution for injection\" together with the antibiotic \"Amoxicillin – 150 suspension\" and in the second ex-perimental group – the drug \"Amoxicillin - 150 suspension\". In all three experi-mental groups, treatment of patients with bronchopneumonia of calves was carried out against the background of vitamin therapy. The content of total protein, albumin and globulin fractions were studied blood among biochemical parameters using an acoustic reagentless computerized analyzer ABKA 01 BIOM. As a result of the study, it was found that application of the proposed medicationsforbronchopneumoniatreatment contributed to a decrease of inflam-matory response, which resulted in activation of redox processes and improvement of metabolic processes in the body.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75359211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF CAPN1C316G GENE POLYMORPHISM ON ECONOMIC AND USEFUL TRAITS OF TWO ECO-GROUPS OF ABERDEEN-ANGUS COWS","authors":"V. Gabidulin, S. Alimova","doi":"10.18286/1816-4501-2022-3-170-174","DOIUrl":"https://doi.org/10.18286/1816-4501-2022-3-170-174","url":null,"abstract":"The article presents results of a study of polymorphic state of CAPN1 C316 G single nucleotide (SNP) gene on economically useful traits of two eco-groups of cows of Aberdeen Angus herd and the ability of their inheritanceby the offspring. The authors found that domestically bred cows were significantly superior to Australian peers in live weight by 9.8% (P≤0.001) and in rump height by 4.1% (P≤0.001). Moreover, the correlation coefficient between live weight and rump height was 0.76, between milk productivity and rump height (negative) - 0.09. In addition, it was revealed that the correlation coefficient between parameters of live weight of cows and live weight of the offspring at the age of 15 months was 0.46, between live weight of cows and rump height of bulls at the age of 15 months was 0.48 and between rump height of cows and bulls - 0.48, in this respect, a significant degree of heritability was 0.21, 0.23 and 0.23, respectively. As a result of CAPN1 C316 gene polymorphism, cows of the GG genotype were significantly superior to their peers of the CG genotypes in terms of live weight - Australian by 4.8% and domestic selection by 2.4% (Р≤0.05). Concurrently, the lowest variability coefficient of breeding traits (such as live weight and height in the rump) was noted in cows Design 1015 homozygous GG genotypes and heterozygous CG, as well as Bismarck 5682 in terms of rump height of GG genotype, which shows the ability of these categories of animals to inherit higher than the marked signs. At the same time, an increase of the G allele proportion in the genotype of cows of Australian and domestic selection contributes to a significant increase in live weight of animals by 6.5% (P≤0.001) and rump height by 2.4% (P≤0.01). Moreover, the dynamics of development of economically useful traits and the ability of their inheritance by cows of Aberdeen Angus breed was established as a genetically determined fact.","PeriodicalId":23563,"journal":{"name":"Vestnik of Ulyanovsk state agricultural academy","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78874894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}