Veterinary microbiology最新文献

{"title":"Molecular insights into Rickettsiales in blood and ticks of two-humped camels at Gansu Province, China: With an accidental detection of Colpodella sp.","authors":"Muhammad Kashif Obaid ,&nbsp;Xinting Lan ,&nbsp;Qiaoyun Ren ,&nbsp;Jehan Zeb ,&nbsp;Jin Luo ,&nbsp;Jifei Yang ,&nbsp;Wenyu Jia ,&nbsp;Xiaoqing Zan ,&nbsp;Hong Yin ,&nbsp;Muhammad Rashid ,&nbsp;Guiquan Guan","doi":"10.1016/j.vetmic.2025.110528","DOIUrl":"10.1016/j.vetmic.2025.110528","url":null,"abstract":"<div><div>Emerging infectious diseases caused by various tick-borne microorganisms (TBMs) pose public and animal health concerns, including camels, with no defined global distribution. In this study, 150 blood samples and 288 ticks were collected from symptomatic two-humped camels (<em>Camelus bactrianus</em>) in Gaotai County, Gansu Province, China. Morphologically identified ticks were confirmed using cytochrome oxidase I (<em>COI</em>), and the findings revealed two species, <em>Hyalomma asiaticum</em> and <em>Haemaphysalis longicornis</em> (prevalence: 245/288 [88.19 %] and 34/288 [11.81 %], respectively). The extracted Genomic DNA from blood and ticks was processed by conventional PCR to investigate the existing TBMs based on <em>16S rRNA</em>, <em>18S rRNA</em>, and <em>17-kDa</em> genes. Different TBMs, including <em>Anaplasma bovis</em>, <em>Colpodella</em> sp., <em>Rickettsia rickettsii</em>, and <em>Candidatus</em> Rickettsia jingxinensis, have been documented as single infections at different rates. High single infection rates (198/218; 90.83 % and 117/150; 78.00 %) of <em>A. bovis</em> in <em>Hy. asiaticum</em> and camel blood were recorded, whereas the lowest single infection rate (3/22; 13.64 %) of <em>R. rickettsii</em> was noted in <em>Hae. longicornis</em>. Co-infection with <em>Rickettsia</em> spp. + <em>A. bovis</em> (20/288; 6.94 %)<em>, Colpodella</em> sp. + <em>A. bovis</em> (14/288; 4.86 %)<em>, Colpodella</em> sp. + <em>Rickettsia</em> spp. (1/288; 0.35 %), and <em>Colpodella</em> sp. + <em>Rickettsia</em> spp. + <em>A. bovis</em> (1/288; 0.35 %) were recorded as concurrent infection. Phylogenetic analysis revealed that the representative TBMs have close similarities and clustered together with their corresponding isolates from China, South Korea, India, the USA, Mexico, Bangladesh, Malawi, Japan, Pakistan, Cyprus, Nigeria, Poland, and Brazil. These findings present a preliminary baseline regarding TBMs infection in camel blood and ticks and provide a framework for further studies on the prevalence and effective control measures for ticks and tick-associated diseases.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110528"},"PeriodicalIF":2.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological characteristics of the recombinant pseudorabies virus with chimeric PCV Cap protein in pigs","authors":"Chenhe Lu ,&nbsp;Wenjing Chen ,&nbsp;Heng Chen ,&nbsp;Gang Xing ,&nbsp;Jiayu Ma ,&nbsp;Hui Zhou ,&nbsp;Linglong Qin ,&nbsp;Liu Da ,&nbsp;Shiping Sun ,&nbsp;Peng Peng ,&nbsp;Haimin Li ,&nbsp;Yulan Jin ,&nbsp;Yan Yan ,&nbsp;Shiyue Pan ,&nbsp;Weiren Dong ,&nbsp;Jinyan Gu ,&nbsp;Jiyong Zhou","doi":"10.1016/j.vetmic.2025.110529","DOIUrl":"10.1016/j.vetmic.2025.110529","url":null,"abstract":"<div><div>Porcine circovirus type 2 (PCV2) is one of the main pathogens causing porcine circovirus-associated diseases (PCVAD). We recently reported the immunogenicity of the recombinant PRV with an envelope-embedded Cap protein of PCV2 (PRV-Cap) in mice. Here, we further evaluated the immunoprotective efficacy of PRV-Cap virus in pigs. Following vaccination, the PRV-Cap stimulated the production of neutralizing antibodies against PRV and PCV2, along with protected piglets from the challenge of the lethal PRV, the virulent PCV2b and PCV2d. Peripheral blood mononuclear cells analysis revealed that PRV-Cap virus effectively induced proliferation and activation of CD4 and CD8 T cells, as well as an increase in T follicular helper cells, although γδ T and B cells did not show significant differences. Compared to DMEM control piglets, the expanded CD4 and CD8 T cells exhibited an effector memory T cell phenotype, and <em>in vitro</em> stimulation led to PRV- and PCV2-specific IFN-γ and TNF-α secretion, peaking at 21 days post-immunization. In summary, PRV-Cap virus effectively prevents PRV, PCV2b and PCV2d challenges in piglets by simultaneously inducing both PRV- and PCV2-specific humoral and cellular immunity, indicating that PRV-Cap virus is a promising and safe candidate vaccine for combined PRV and PCV2 immunization.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110529"},"PeriodicalIF":2.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat-inactivated Mycobacterium bovis and P22PI protein immunocomplex: Two candidates for use as immunostimulants of innate immune response","authors":"Irene Agulló-Ros ,&nbsp;Mercedes M. Burucúa ,&nbsp;Felipe A. Cheuquepán ,&nbsp;Mercedes Domínguez ,&nbsp;Iker A. Sevilla ,&nbsp;Remigio Martínez ,&nbsp;Natalia Plá ,&nbsp;María A. Risalde ,&nbsp;Maia S. Marin","doi":"10.1016/j.vetmic.2025.110527","DOIUrl":"10.1016/j.vetmic.2025.110527","url":null,"abstract":"<div><div>Tuberculosis (TB), caused by members of the <em>Mycobacterium tuberculosis complex</em>, remains a critical global health challenge, affecting humans and a wide range of domestic and wild animals. Despite the availability of anti-TB drugs, cure rates remain suboptimal, exacerbated by the rise of multidrug-resistant TB strains. The Bacille Calmette-Guérin (BCG) vaccine, the only licensed vaccine against TB, has demonstrated efficacy in reducing lesion severity and bacterial burden in animals, as well as lowering TB-related and all-cause mortality in infants. However, BCG presents several safety concerns inherent to live vaccines. To overcome these limitations, exploring alternative vaccine candidates that do not incorporate live mycobacteria is crucial. This study aimed to evaluate and compare the immunostimulatory potential of two candidates based in mycobacteria inactivated or their derivatives, heat-inactivated <em>Mycobacterium bovis</em> (HIMB) and P22PI protein immunocomplex (P22PI), in bovine foetal lung cells. To assess the expression of innate immune components, including Toll-like receptors (TLRs), cathelicidins, and cytokines, bovine foetal lung were exposed to different concentrations of HIMB and P22PI immunostimulants, starting at 7.8 × 10⁶ CFU/ml and 10 µg/ml, respectively. These initial concentrations were subsequently diluted to 1/2 and 1/10 to evaluate dose-dependent effects. Our findings reveal that both HIMB and P22PI significantly stimulate innate immune mechanisms, as evidenced by the upregulation of TLR2 and TLR4, alongside the induction of BMAP28 cathelicidin, tumour necrosis factor alpha (<em>TNFA</em>) and interferons (IFNs). These results suggest their potential to orchestrate a robust innate immune response providing valuable insights into the immunological mechanisms underlying the protective effects of these immunostimulants. This underscores their potential role in <em>in vivo</em> studies as vaccine candidates. Furthermore, their ability to enhance antigen recognition via TLR and induce pro-inflammatory cytokines also indicates broader applications in immune modulation, potentially extending protection against heterologous pathogens through trained immunity.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110527"},"PeriodicalIF":2.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The kinetics of gene expression related to innate and adaptive immunity in the lung and spleen following Newcastle disease virus (NDV) infection in vaccinated broiler chickens employing different vaccination regimes","authors":"Ola Hassanin ,&nbsp;Fatma Abdallah ,&nbsp;Mahmoud HA Mohamed ,&nbsp;Mohamed S. Ahmed ,&nbsp;Mohammed Al-Rasheed ,&nbsp;Eman M. Rashad","doi":"10.1016/j.vetmic.2025.110525","DOIUrl":"10.1016/j.vetmic.2025.110525","url":null,"abstract":"<div><div>Newcastle disease virus (NDV) classified as an avian paramyxovirus-1 poses a significant risk to the global poultry sector. This study explored the kinetics of the innate and adaptive immune responses in chickens exposed to virulent Newcastle Disease Virus (vNDV) genotype VII. A challenge experiment was carried out with both non-vaccinated and NDV-vaccinated chickens utilizing different vaccination strategies commonly used in the industry. The four groups of vaccinated birds were administered either two doses of live NDV vaccines, live vaccines boosted with an inactivated vaccine, the rHVT-NDV-IBDV vaccine alone, or the rHVT-NDV-IBDV vaccine in conjunction with a live vaccine booster. To assess seven cytokines linked to antiviral and proinflammatory innate responses quantitative real-time polymerase chain reaction (qRT-PCR) analyses were implemented. The analyses revealed robust innate immune responses in all cytokines measured in lung and spleen tissues of the group that received both live and inactivated vaccines. Notably these increases were found to correlate with the humoral immune response within that same group. Significant transcriptional activity in the lung and spleen tissues of non-vaccinated chickens at 24 hour post-infection (pi). The most significant upregulations were identified in toll-like receptor 7 (TLR7), TLR5, interleukin 6 (IL6), chicken interferon-alpha (chIFN-α), and myxovirus resistance protein 1 (Mx1), which later exhibited a regression. The administration of both live and inactivated vaccines has proven effective in restoring the suppression or inhibition of vNDV infection across a wide range of cytokines, including TLR7, TLR5, chIFN-α, Mx1, IL6, and MHC-1. Various vaccination strategies have been shown to either mitigate or prevent cytokine storms within 24 hour pi in the lungs, spleen, or both, in certain cytokines such as chIFN-α, Mx1, and IL6. Collectively these results suggest that different vaccination strategies modify the kinetics and pathophysiological responses associated with vNDV infection in chickens.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110525"},"PeriodicalIF":2.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of host factors affecting Chicken Infectious Anemia Virus infection and pathogenicity through RNA-seq and LC-MS","authors":"Moru Xu ,&nbsp;Nana Tian ,&nbsp;Yupeng Liu ,&nbsp;Kun Qian ,&nbsp;Hongxia Shao ,&nbsp;Jianqiang Ye ,&nbsp;Aijian Qin","doi":"10.1016/j.vetmic.2025.110524","DOIUrl":"10.1016/j.vetmic.2025.110524","url":null,"abstract":"<div><div>Chicken Infectious Anemia Virus (CIAV) infection causes severe anemia, hematopoietic disorders, and immunosuppression in poultry. However, the mechanisms underlying its pathogenicity remain poorly understood. In this study, RNA sequencing (RNA-seq) was employed to investigate the transcriptional response of MSB1 cells to CIAV infection at 12, 24, and 48 hours post-infection. The results revealed differential gene expression associated with immune response, inflammatory response, apoptosis, and metabolic pathways. Several immune-related factors, including IL18, TRAF1, MYD88, IRF2, and CD28, were found to be downregulated. VP1, the capsid protein of CIAV, plays a significant role in viral pathogenicity. To explore how VP1 affects CIAV replication and pathogenicity, co-immunoprecipitation (Co-IP) was used to identify host proteins interacting with VP1. Liquid chromatography-mass spectrometry (LC-MS) analysis revealed that VP1 interacts with a range of host proteins involved in metabolic pathways, the PI3K-AKT signaling pathway, and ribosomal functions. Based on RNA-seq and LC-MS findings, ANXA6 and HSP90aa1 were identified as key interacting partners, which co-localize in the cytoplasm of infected cells. Both proteins are involved in immune responses and metabolic regulation and were found to be downregulated in CIAV-infected cells. Notably, overexpression of ANXA6 or HSP90aa1 inhibited CIAV infection during the early stages of the viral lifecycle. These findings enhance our understanding of the host factors influencing CIAV infection and provide new insights into potential therapeutic targets for managing CIAV-induced diseases.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110524"},"PeriodicalIF":2.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome and metabolome alterations in calves with enterohemorrhagic Escherichia coli and dyspeptic diarrhea","authors":"Lantian Huang ,&nbsp;Zhengyu Lin ,&nbsp;Xinyi Zhu ,&nbsp;Long Guo ,&nbsp;Luying Cui ,&nbsp;Junsheng Dong ,&nbsp;Kangjun Liu ,&nbsp;Jianji Li ,&nbsp;Heng Wang","doi":"10.1016/j.vetmic.2025.110523","DOIUrl":"10.1016/j.vetmic.2025.110523","url":null,"abstract":"<div><div>Enterohemorrhagic <em>Escherichia coli</em> (EHEC) and dyspeptic diarrhea are significant health concerns in calves, leading to substantial economic losses in the livestock industry. This study investigated the impact of EHEC infection and dyspeptic diarrhea on calf health, focusing on blood parameters, fecal microbiota, and metabolite profiles. Thirty-two holstein calves were divided into three groups: healthy group (C Group), EHEC-infected group (E Group), and indigestion-induced diarrhea group (I Group). Significant alterations in diarrheic calves were noted in peripheral blood parameters, including hematological, biochemical, and blood gas indices. And then fecal microbiota analysis revealed decreased diversity, with reduced <em>Actinobacteria</em> and increased <em>Proteobacteria</em> and <em>Fusobacteriota</em> in E and I group. Metabolomic profiling showed significant reductions in organic acids and lipids in diarrheic calves. The study concludes that microbial and metabolic alterations play critical roles in the pathogenesis of EHEC- and indigestion-induced diarrhea, with Scorzoside identified as a potential biomarker for differentiating healthy calves from those with diarrhea. These findings provide insights for designing targeted interventions to enhance gut health and reduce disease burden in the livestock.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110523"},"PeriodicalIF":2.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cepharanthine mitigates NIBV-induced pyroptosis via the MDA5/NF-κB/NLRP3 signaling pathway","authors":"Zhenni Liu ,&nbsp;Ruoyun Tang ,&nbsp;Mengbing Ding,&nbsp;Meiqin Wu,&nbsp;Ying Li,&nbsp;Cheng Huang,&nbsp;Qiurong Qi,&nbsp;Ping Liu,&nbsp;Xiaona Gao,&nbsp;Xiaoquan Guo","doi":"10.1016/j.vetmic.2025.110519","DOIUrl":"10.1016/j.vetmic.2025.110519","url":null,"abstract":"<div><div>This study elucidates the mechanism through which cepharanthine (CEP) mitigates NIBV-induced pyrodeath in renal tubular epithelial cells by modulating the MDA5/NF-κB/NLRP3 signaling pathway. Primary renal tubular epithelial cells (RTECs) were isolated from one-day-old Hy-Line Brown chicks and subsequently assigned to four groups. The C+CEP and N + CEP groups received 0.5 μM CEP, while the NIBV and N + CEP groups were exposed to 1 MOI of the SX9 strain of NIBV. The study shows that CEP significantly reduces NIBV load (<em>P</em> &lt; 0.05) and decreases the mRNA and protein levels of MDA5/NF-κB/NLRP3 signaling components, such as MDA5, ISP-1, TRAF6, TAK1, NF-κB, IKKα, IKKβ, NLRP3, caspase-1, IL-1β, and IL-18, upon NIBV infection (<em>P</em> &lt; 0.05 or <em>P</em> &lt; 0.01). Moreover, CE<em>P</em> markedly reduced cellular pyroptosis (<em>P</em> &lt; 0.001) and lactate dehydrogenase (LDH) activity (<em>P</em> &lt; 0.01). Flow cytometry and fluorescence-based assays corroborated these findings, revealing a substantial diminution in pyroptosis post-NIBV infection in CEP-treated cells (<em>P</em> &lt; 0.01). Laser confocal microscopy revealed a clear decrease in NLRP3-associated red fluorescence foci after CEP treatment. These results highlight that CEP could mitigate NIBV-induced pyroptosis in RTECs by modulating the MDA5/NF-κB/NLRP3 signaling pathway.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110519"},"PeriodicalIF":2.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcus suis manganese transporter mutant as a live attenuated vaccine: Safety, efficacy, and virulence reversion mechanisms","authors":"Michelle Wiebe,&nbsp;Alaina Ingebritson,&nbsp;Melody Sholeh,&nbsp;Corrie Tichenor,&nbsp;Callie Visek,&nbsp;Joseph Victoria,&nbsp;Michael Beck,&nbsp;Raksha Tiwari,&nbsp;Philip Hardwidge,&nbsp;Luchang Zhu","doi":"10.1016/j.vetmic.2025.110521","DOIUrl":"10.1016/j.vetmic.2025.110521","url":null,"abstract":"<div><div>Streptococcus suis is the leading cause of mortality in piglets and is responsible for severe economic losses in the global pork industry. Severe invasive diseases caused by S. suis include sepsis, meningitis, arthritis, and endocarditis. S. suis disease prevention is hampered by the lack of safe and efficacious vaccines. In this study, we constructed an S. suis live attenuated vaccine candidate lacking the major streptococcal manganese transporter, a known virulence determinant of this organism. The safety and efficacy of this live vaccine were evaluated in swine. Our clinical study results showed that when administered at a dose of 10¹⁰ CFU, the vaccine strain was safe and efficacious. However, a lower dose of 10⁹ CFU failed to generate significant immune protection. To investigate if an adjuvant could enhance the efficacy of the vaccine at a lower dose, we spiked the vaccine with a polymeric adjuvant and evaluated its performance. Surprisingly, four pigs receiving the adjuvanted vaccine died during the vaccination phase. Pathology, microbiology, and genetic analyses suggested that the vaccine strain reverted to virulence in these animals. Functional genetic analysis found that the vaccine strain acquired compensatory mutations that upregulated the expression of a secondary manganese transporter, which in turn restored the virulence of the vaccine strain. Our results provide a new understanding of S. suis host adaptation mechanisms and useful information for the design of future live-attenuated vaccines.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110521"},"PeriodicalIF":2.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First isolation of Leptospira interrogans from follicular fluid of naturally infected cows","authors":"Ana Luiza dos Santos Baptista Borges ,&nbsp;Luiza Aymée ,&nbsp;Isabel Roussouliéres ,&nbsp;Filipe Anibal Carvalho-Costa ,&nbsp;Maria Isabel Nogueira Di Azevedo ,&nbsp;Walter Lilenbaum","doi":"10.1016/j.vetmic.2025.110522","DOIUrl":"10.1016/j.vetmic.2025.110522","url":null,"abstract":"<div><div>The presence of leptospires in the follicular fluid has only been confirmed through molecular techniques, as culturing leptospires is extremely challenging. The lack of studies demonstrating the viability of leptospires in this site limits a deeper understanding of pathogenesis. Therefore, this study aimed to cultivate and molecularly characterize <em>Leptospira</em> spp. from follicular fluid and uterine tissue samples collected from naturally infected cows. A total of 85 cows from herds with leptospirosis were selected and 53 follicular fluids (FF) and 85 uterine fragments (UF) were collected after slaughter. The samples were seeded into T80/40LH, and evaluated by Darkfield Microscopy (DFM). Positive cultures were tested by <em>lipL32</em>-PCR to confirm the presence of pathogenic <em>Leptospira</em> spp. Positive tubes were submitted to serogrouping and genotyping by sequencing of the <em>secY</em> gene. A maximum likelihood (ML) tree was constructed. A total of 33/85 (39 %) cows were positive, 16/53 (30.2%) only in FF, 14/85 (16.4 %) only in UF, and three in both samples. It was possible to obtain one isolate from FF, serogrouped as Icterohaemorrhagiae. Six samples were sequenced by <em>sec</em>Y. All of them were identified as <em>L. interrogans</em>, with &gt; 99 % identity. The ML tree revealed that all sequences belong to a group with strains close to serovar Hardjo. Herein, we highlight the presence of live <em>L. interrogans</em> in follicular fluid, emphasizing it as an important site of infection, as leptospires could impair embryo production. The similarity of the strains involved to highly virulent strains in humans raises concerns, posing a potential zoonotic risk.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110522"},"PeriodicalIF":2.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Critical role for heat shock protein 70 in viral replication of ALV-J via interaction with gp37 and P32,” [Vet. Microbiol. 303 (2025) 110436]","authors":"Kensi Zhu,&nbsp;Yanling Pang,&nbsp;Zhihong Luo,&nbsp;Hanyue Zhang,&nbsp;Shuang Tang,&nbsp;Jinjie Liang,&nbsp;Yuecheng Long,&nbsp;Wencheng Lin","doi":"10.1016/j.vetmic.2025.110510","DOIUrl":"10.1016/j.vetmic.2025.110510","url":null,"abstract":"","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"305 ","pages":"Article 110510"},"PeriodicalIF":2.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}