Veterinary microbiology最新文献

{"title":"Critical role for heat shock protein 70 in viral replication of ALV-J via interaction with gp37 and P32","authors":"Kensi Zhu ,&nbsp;Yanling Pang ,&nbsp;Zhihong Luo ,&nbsp;Hanyue Zhang,&nbsp;Shuang Tang,&nbsp;Jinjie Liang,&nbsp;Yuecheng Long,&nbsp;Wencheng Lin","doi":"10.1016/j.vetmic.2025.110436","DOIUrl":"10.1016/j.vetmic.2025.110436","url":null,"abstract":"<div><div>Avian leukemia virus subgroup J (ALV-J) causes various diseases associated with tumor formation, decreased fertility and immunosuppression, resulting in significant economic losses in the poultry industry globally. Virus usually exploits the host cellular machinery for their replication. Although there are increasing evidences for the cellular proteins involving viral replication, the interaction between ALV-J and host proteins leading to the pivotal steps of viral life cycle are still unclear. Here, we reported that the heat shock protein 70 (Hsp70) plays a critical role during ALV-J infection by interacting with gp37 and P32. Changing the expression of Hsp70 affects the replication of ALV-J in host cells, and inhibitory of Hsp70 using the specific inhibitors JG-98 or Pifithrin-μ significantly reduced viral replication. This study revealed the effect of host Hsp70 on ALV-J replication, providing insights for further studies of the molecular mechanism of ALV-J infection.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"303 ","pages":"Article 110436"},"PeriodicalIF":2.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a multiplex PCR assay for detection of Riemerella anatipestifer serotype 1 and serotype 2 strains","authors":"Mengsi Wu ,&nbsp;Rong Guo ,&nbsp;Meitong Chen ,&nbsp;Min Zhu ,&nbsp;Zongchao Chen ,&nbsp;Chan Ding ,&nbsp;Shengqing Yu","doi":"10.1016/j.vetmic.2025.110435","DOIUrl":"10.1016/j.vetmic.2025.110435","url":null,"abstract":"<div><div><em>Riemerella anatipestifer</em> infection is one of the main infectious diseases that threaten the poultry industry at present. Serotypes 1 and 2 are the main serotypes causing global outbreaks of <em>R. anatipestifer</em>. In this study, we designed the primers specific for <em>R. anatipestifer</em> serotypes 1 and serotype 2 strains, and for <em>R. anatipestifer</em> 16S rRNA (for the species identification) to establish a multiplex PCR assay for rapid detection of the serotype 1 and serotype 2 strains. The specificity test showed that a 505 bp fragment was amplified from serotype 1 strains, and a 1125 bp fragment was amplified from serotype 2 strains. A 843 bp fragment of 16S rRNA was amplified from <em>R. anatipestifer</em> strains with different serotypes. No amplification band was shown for other species of bacterial pathogens, including <em>Escherichia coli</em>, <em>Salmonella, Pasteurella multocida</em>, <em>Mycoplasma gallisepticum</em> and <em>Mycoplasma synoviae</em>. The sensitivity test showed a detection limit of 10² CFU of the multiplex PCR assay. Furthermore, a total of 60 <em>R. anatipestifer</em> clinical isolates were tested for identification of the serotypes, and the results showed that a 843 bp 16S rRNA fragment was amplified from all 60 isolates, confirming they are <em>R. anatipestifer</em> strains. Moreover, a 505 bp serotype 1 fragment was amplified from 9 isolates, and a 1125 bp serotype 2 fragment was amplified from 28 isolates. <em>R. anatipestifer</em> serotype 1 and 2 strains accounted for 61.67 % of the isolates. The results were further validated by slide agglutination test, which showed 100 % consistency with the multiplex PCR.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"303 ","pages":"Article 110435"},"PeriodicalIF":2.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characteristics and antimicrobial resistance of the underreported zoonotic pathogen Streptococcus pasteurianus and its co-colonization with Streptococcus suis","authors":"Shuoyue Wang ,&nbsp;Xinchun Li ,&nbsp;Chenxu Zheng ,&nbsp;Juan J. Quereda ,&nbsp;Jie Sun ,&nbsp;Huochun Yao ,&nbsp;Zongfu Wu","doi":"10.1016/j.vetmic.2025.110428","DOIUrl":"10.1016/j.vetmic.2025.110428","url":null,"abstract":"<div><div><em>Streptococcus pasteurianus</em> is an opportunistic pathogen affecting various animals and is an underreported zoonotic threat. It is also a causative agent of swine streptococcosis and can be co-detected with <em>Streptococcus suis</em>, another significant pig and zoonotic pathogen. However, the dynamics of co-colonization between these pathogens, along with the genomic features and antibiotic resistance profiles of <em>S. pasteurianus</em>, remain poorly understood. In this study, we developed a multiplex PCR (mPCR) assay to detect <em>S. pasteurianus</em> and <em>S. suis</em> in 827 tonsil samples from healthy pigs, with 81 samples positive for both pathogens. Pan-genome analysis revealed an open pan-genome, indicating an adaptable genome. Antibiotic resistance gene analysis identified 21 distinct genes, including the first identification of <em>mef(A)</em>, <em>msr(D)</em>, <em>optrA</em>, <em>lnu(G)</em>, <em>spw</em>, <em>dfrF</em>, and <em>fexA</em> in <em>S. pasteurianus</em>. Strain WUSP082 carried 15 resistance genes, many of which were located on mobile genetic elements. ICE_WUSP082–1 carries <em>tet(O)</em>, <em>erm(B)</em>, and <em>ant(6)-Ia</em>, showing 99.10 % sequence similarity to <em>S. suis</em> ICE_{SsuZJ20091101–1}. GI_WUSP082–1, containing <em>tet(L)</em> and <em>tet(M)</em>, shares 99.94 % similarity with <em>S. suis</em> 89-kb pathogenicity island. Plasmid_WUSP082, carrying <em>fexA</em>, <em>optrA</em>, and <em>erm(A)</em>, shares 98.85 % sequence homology with <em>Enterococcus faecium</em> plasmid pW6–2. All 15 strains collected from our lab displayed multidrug resistance, being resistant to at least four classes of antibiotics. Mouse infection experiments demonstrated the pathogenic potential of WUSP082, isolated from the tonsil of a healthy pig. This study advances our understanding of the genomic characteristics and antimicrobial resistance of <em>S. pasteurianus</em>, offering valuable insights for the surveillance and management of this under-recognized zoonotic pathogen.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"303 ","pages":"Article 110428"},"PeriodicalIF":2.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porcine epidemic diarrhea virus induces mitophagy to inhibit the apoptosis and activation of JAK/STAT1 pathway","authors":"Xin Li ,&nbsp;Yiwan Wu ,&nbsp;Jin Peng ,&nbsp;Bingjie Li ,&nbsp;XiaoLong Li ,&nbsp;Zhibin Yan ,&nbsp;Gen Li ,&nbsp;Yue Zhang ,&nbsp;HongLing He ,&nbsp;Jun Luo ,&nbsp;Xiaofeng Guo","doi":"10.1016/j.vetmic.2025.110427","DOIUrl":"10.1016/j.vetmic.2025.110427","url":null,"abstract":"<div><div>Porcine epidemic diarrhea virus (PEDV) infection leads to immunosuppression and clinical symptoms in piglets, including vomiting, watery diarrhea, dehydration, and even death. Mitophagy sustains mitochondrial energy homeostasis and quality through the removal of damaged mitochondria. However, PEDV disrupts mitochondrial homeostasis, which affects cellular energy supply and reproduction. Despite existing research, the mechanisms underlying PEDV pathogenesis and its interaction with the innate immune system remain largely unclear. Therefore, we aimed to clarify the mechanism of PEDV-induced mitophagy and its relationship with apoptosis and Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway after PEDV infection. We infected Vero and IPEC-J2 cells with PEDV. Then, we evaluated mitochondrial morphology, structural proteins of PEDV, reactive oxygen species (ROS) levels, and mitochondrial membrane potential using transmission electron microscopy, confocal laser scanning microscopy, and flow cytometry. We identified mitophagy-related proteins through immunoprecipitation and western blotting. We examined the effects of mitophagy on PEDV proliferation and JAK1-STAT1 signaling via western blotting and indirect immunofluorescence. PEDV infection led to mitochondrial damage and the production of mitophagosome-like vesicles. Subsequently, the PEDV structural N protein initiated mitophagy through ubiquitinating mitofusin 2 (MNF2) via the PINK1/Parkin pathway. Moreover, mitophagy promoted PEDV replication. In the early stage of PEDV infection, PEDV infection inhibits apoptosis by promoting mitophagy. PEDV infection significantly decreased the expression of JAK1, STAT1, interferon regulatory factor 9, and phosphorylated STAT1, inhibiting nuclear translocation and promoting replication. Overall, PINK1/Parkin-mediated mitophagy regulated PEDV-induced apoptosis and JAK/STAT1 expression. These findings provide a scientific basis for elucidating the pathogenic and immune escape mechanisms of PEDV.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"303 ","pages":"Article 110427"},"PeriodicalIF":2.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole transcriptome analysis of Mycoplasma bovis-host interactions under in vitro and in vivo conditions","authors":"Aga E. Gelgie ,&nbsp;Benti D. Gelalcha ,&nbsp;Trevor Freeman ,&nbsp;Taylor B. Ault-Seay ,&nbsp;Jonathan Beever ,&nbsp;Oudessa Kerro Dego","doi":"10.1016/j.vetmic.2025.110426","DOIUrl":"10.1016/j.vetmic.2025.110426","url":null,"abstract":"<div><div><em>Mycoplasma bovis</em> mastitis is becoming increasingly problematic for dairy cattle farming. <em>M. bovis</em> is inherently resistant to beta-lactam antimicrobials and no effective vaccine is available. The major constraints to developing effective control tools are limited knowledge of <em>M. bovis</em> virulence factors and the underlying pathogenic mechanisms. The objective of this study was to determine virulence-associated genes of <em>M. bovis</em> and host immune response genes expressed during the early stages of host-pathogen interactions. We conducted <em>in vitro</em> infection of mammary epithelial cell (MAC-T) lines and <em>in vivo</em> intramammary infection of dairy cows with <em>M. bovis</em> strain PG45 and evaluated whole transcriptome differential gene expression. A total of 614 and 7161 genes of <em>M. bovis</em> and bovine host cells were differentially expressed, respectively. Insertion sequence (IS) genes that are involved in transposase activity such as ISMbov1, ISMbov2, ISMbov3, and ISMbov9 were significantly upregulated, whereas protein translation-associated genes were significantly downregulated. In MAC-T cells, genes involved in apoptosis pathways and proinflammatory cytokines were significantly upregulated, whereas genes involved in cell cycle, ribosome biogenesis, and steroid biosynthesis were significantly downregulated. Genes encoding formation of neutrophil extracellular traps and proinflammatory cytokines, were significantly upregulated in the mammary gland of <em>M. bovis</em> challenged cows, whereas genes involved in steroid biosynthesis and metabolism were significantly downregulated. Altogether, while our findings shed light on the simultaneous transcriptional changes in <em>M. bovis</em> and the host during infection, further studies are required to understand a complete picture of these interactions that lead to mastitis.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"303 ","pages":"Article 110426"},"PeriodicalIF":2.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of individual and population-based sampling for detection of influenza A virus RNA in breeding swine herds","authors":"DCA Moraes ,&nbsp;PC Gauger ,&nbsp;OH Osemeke ,&nbsp;IF Machado ,&nbsp;G Cezar ,&nbsp;RC Paiva ,&nbsp;MP Mil-Homens ,&nbsp;MN Almeida ,&nbsp;A Ramirez ,&nbsp;GS Silva ,&nbsp;DCL Linhares","doi":"10.1016/j.vetmic.2025.110423","DOIUrl":"10.1016/j.vetmic.2025.110423","url":null,"abstract":"<div><div>Sample types currently used for Influenza A virus (IAV) surveillance in swine farms vary in sensitivity, convenience of collection, and herd representativeness. Family oral fluids are an effective population-based sample type for detecting porcine reproductive and respiratory syndrome virus (PRRSV) (ribonucleic acid) RNA by real-time reverse transcription–polymerase chain reaction (RT-rtPCR) in breeding herds. However, little is known about the efficacy of family oral fluids samples for detecting IAV RNA in these herds. This study compared the probability of IAV RNA detection among individual and population-based samples. A 3,500-sow breeding herd was sampled for matched sets (n = 57) of family oral fluids, udder wipes, sow nasal wipes, individual piglet nasal wipes, and drinker wipes, tested by RT-rtPCR for IAV RNA. Overall, 57.9 % (33/57) of family oral fluids, 49.1 % (28/57) of udder wipes, 28.1 % (16/57) of sow nasal wipes, 15.8 % (9/57) of drinker wipes, and 66.6 % (38/57) of individual piglet nasal wipes were positive. Family oral fluids showed a Kappa value of 0.81, indicating near-perfect agreement with individual piglet nasal wipes, while udder wipes had a substantial agreement (Kappa = 0.65). Other sample types showed fair agreement (Kappa &lt; 0.28). These results validate family oral fluids as an efficient alternative population-based sample for IAV surveillance in breeding herds. The proportion of positive piglets within litters by room was 91 % in room A (20/22), 70 % in room B (17/24), and 9 % in room C (1/11). This study also highlights the importance of sampling different farrowing rooms within the same breeding herd to enhance IAV surveillance.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"302 ","pages":"Article 110423"},"PeriodicalIF":2.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biphasic modulation of the TLR7 signaling pathway in bovine alphaherpesvirus (BoAHV) infection of neural cells","authors":"J.J. Rosales ,&nbsp;M.B. Brunner ,&nbsp;M.S. Marin ,&nbsp;S.E. Pérez","doi":"10.1016/j.vetmic.2025.110424","DOIUrl":"10.1016/j.vetmic.2025.110424","url":null,"abstract":"<div><div>The study investigates the role of TLR7 in the modulation of the immune response during infection of neuronal cells by bovine alphaherpesvirus (BoAHV) types 1 and 5. TLR7 is essential for detecting viral RNA and activating immune pathways. In BoAHV-1 infection, TLR7 is upregulated early and persistently. In contrast, BoAHV-5 initially suppresses TLR7 expression, with a delayed upregulation at the end of the infectious cycle, reflecting the ability of the virus to evade early immune detection. Furthermore, BoAHV-1 induces a strong activation of MyD88 and NF-κB, leading to rapid viral replication, while BoAHV-5 triggers a weaker immune response, resulting in slower viral replication during the initial hours of infection. Additionally, BoAHV-1 progressively activates IRF-7 whereas BoAHV-5 shows delayed IRF-7 activation. Nevertheless, BoAHV-5 induces a strong IFNα/β response. The antiviral effect of the TLR7 agonist, Imiquimod was evident at the late phase of BoAHV-5 infection and it was mediated by IFN-β. These findings suggest that targeting TLR7 signaling could be a potential therapeutic approach to modulate immune responses and control viral replication. However, the effectiveness of TLR7 agonists like Imiquimod may vary depending on the virus type and its immune evasion strategies, highlighting the need for further research to explore other molecules in the TLR7 pathway.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"302 ","pages":"Article 110424"},"PeriodicalIF":2.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel recombinant chimeric Fiber-8a/8b-AD subunit vaccine provides complete protection against both FAdV-8a and FAdV-8b","authors":"Xiangqin Wang ,&nbsp;Wanzhe Yuan","doi":"10.1016/j.vetmic.2025.110425","DOIUrl":"10.1016/j.vetmic.2025.110425","url":null,"abstract":"<div><div>In recent years, outbreaks of fowl adenovirus (FAdV) in domestic chicken populations in China, particularly fowl adenovirus serotype 8 (FAdV-8), have attracted significant attention due to its role as a major causative agent of inclusion body hepatitis (IBH). Although FAdV-8 exhibits lower virulence compared to FAdV-4, its ability to co-infect with other pathogens presents new challenges for vaccine development. In this study, full-length and chimeric expression technologies were applied to develop four novel subunit vaccines using Fiber proteins from FAdV-8a and FAdV-8b strains: Fiber-8a-AB, Fiber-8b-CD, Fiber-8a/8b-AD, and Fiber-8b/8a-CB. Immunogenicity experiments indicated that the Fiber-8a/8b-AD subunit vaccine induced production of antibodies at 14 days of age earlier than the other three subunit vaccine. Moreover, the neutralizing antibody level of the Fiber-8a/8b-AD subunit vaccine group was higher than the three subunit vaccine groups. Immuno-challenge tests confirmed that this vaccine effectively protected chickens from weight loss, liver damage, and viral shedding post FAdV-8a and FAdV-8b infection. These findings valuable crucial insights for the development of new vaccines and suggest that the Fiber-8a/8b-AD subunit vaccine has the potential to serve as an effective alternative to inactivated whole-virus vaccines, with substantial commercial potential.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"303 ","pages":"Article 110425"},"PeriodicalIF":2.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence determinants in Klebsiella pneumoniae associated with septicaemia outbreaks in neonatal pigs","authors":"Alison M. Collins,&nbsp;Rachel Mizzi","doi":"10.1016/j.vetmic.2025.110409","DOIUrl":"10.1016/j.vetmic.2025.110409","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> is recognized as an opportunistic pathogen in pigs causing pneumonia, mastitis and diarrhoea, but can also cause mortalities due to septicaemia and meningitis in previously healthy piglets. This study aimed to identify virulence genes present in <em>K. pneumoniae</em> that caused outbreaks of septicaemia in neonatal pigs. The genomes of thirty-eight Australian <em>K. pneumoniae</em> isolates from pigs with septicaemia, meningitis, myocarditis, pneumonia, enteritis and healthy cohorts were sequenced. The presence of antimicrobial resistance, siderophore and enhanced capsule production genes were identified by sequence analysis and verified by either PCR or phenotypic tests. An additional 52 international <em>K. pneumoniae</em> genomes from healthy and clinically affected pigs (28), humans (16), birds (3), one rodent and environmental isolates (4) were included in a pangenome analysis. Porcine septicaemic <em>K. pneumoniae</em> genomes from the UK and Australia clustered together and had higher virulence scores than all other clinical and non-clinical isolates. Septicaemic isolates were predominantly ST25, had enhanced capsule polysaccharide production with K2 capsule type and contained genes for the siderophores aerobactin, salmochelin and yersiniabactin. Septicaemic <em>K. pneumoniae</em> were more likely to have genes encoding the assembly of LPS, fimbriae and adhesins, and enzymes needed for the integration of mobile genetic elements<em>.</em> No single virulence gene was solely associated with isolates causing septicaemia. These findings indicate that there may be genotypes associated with clinical disease outcomes for <em>K. pneumoniae</em>. In the absence of some virulence genes, <em>K. pneumoniae</em> was still able to cause significant disease if the pig’s immune system was immature or compromised.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"302 ","pages":"Article 110409"},"PeriodicalIF":2.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance patterns and biofilms resurgence ability of Escherichia coli associated with commercial layer chicken farms in Sri Lanka","authors":"Iresha Subhasinghe ,&nbsp;H.M.H.N. Bandara ,&nbsp;H.M.T.K. Karunarathna ,&nbsp;S.P. Kodithuwakku ,&nbsp;H.C. Gallage ,&nbsp;R.S. Kalupahana ,&nbsp;K.S.A. Kottawatta","doi":"10.1016/j.vetmic.2025.110422","DOIUrl":"10.1016/j.vetmic.2025.110422","url":null,"abstract":"<div><div>This study aims to understand antimicrobial resistance (AMR) associated with planktonic and biofilm-forming bacteria in Sri Lankan chicken industry. Planktonic or biofilm-forming <em>Escherichia coli</em> were isolated from different sources in layer chicken farms and their AMR profiles were determined. Minimum inhibitory concentrations (MICs) of selected antibiotics were quantified against planktonic <em>E. coli</em> isolated from water. Minimum biofilm inhibitory/eradication concentrations (MBIC/MBEC) of tetracycline were determined for biofilm <em>E. coli</em>. Faecal <em>E. coli</em> demonstrated highest resistance to tetracycline (64 % of isolates). Biofilm-derived planktonic <em>E. coli</em> showed greater resistance to antibiotics than planktonic <em>E. coli</em> (75 % vs 62.5 %)<em>.</em> The MBIC and MBEC of tetracycline in <em>E. coli</em> biofilm phenotype were significantly greater than MIC of planktonic phenotype of the same isolate (P &lt; 0.05). This study highlights the importance of eliminating biofilms in chicken industry as biofilm-forming bacteria isolated from drinkers demonstrated a significantly greater AMR and can act as a source of AMR dissemination.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"302 ","pages":"Article 110422"},"PeriodicalIF":2.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}