Veterinary microbiology最新文献_第10页

Duck plague virus-encoded microRNA dev-miR-D28-3p inhibits viral replication via targeting UL27 鸭瘟病毒编码的微RNA dev-miR-D28-3p通过靶向UL27抑制病毒复制。

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-29 DOI: 10.1016/j.vetmic.2024.110202

Hui Ni , Xingcui Zhang , Juan Huang , Mingshu Wang , Anchun Cheng , Mafeng Liu , Dekang Zhu , Shun Chen , Xinxin Zhao , Qiao Yang , Ying Wu , Shaqiu Zhang , Xumin Ou , Di Sun , Bin Tian , Bo Jing , Renyong Jia

{"title":"Duck plague virus-encoded microRNA dev-miR-D28-3p inhibits viral replication via targeting UL27","authors":"Hui Ni , Xingcui Zhang , Juan Huang , Mingshu Wang , Anchun Cheng , Mafeng Liu , Dekang Zhu , Shun Chen , Xinxin Zhao , Qiao Yang , Ying Wu , Shaqiu Zhang , Xumin Ou , Di Sun , Bin Tian , Bo Jing , Renyong Jia","doi":"10.1016/j.vetmic.2024.110202","DOIUrl":"10.1016/j.vetmic.2024.110202","url":null,"abstract":"<div>Herpesviruses-encoded microRNAs (miRNAs) have been discovered to be essential regulators in viral life cycle, participating in viral replication, latent or lytic infection, and immunological escape. However, the roles of miRNAs encoded by duck plague virus (DPV) are still unknown. Dev-miR-D28-3p is a miRNA uniquely encoded by DPV CHv strain. The aim of this study was to explore the effect of dev-miR-D28-3p on DPV replication and explore the potential mechanisms involved. Our findings demonstrated that transfection of dev-miR-D28-3p mimic into duck embryo fibroblasts (DEFs) effectively suppressed viral copies, viral titers and viral protein expressions during DPV infection, while the results above were reversed after transfection with dev-miR-D28-3p inhibitor. Subsequently, we further discovered that dev-miR-D28-3p specifically bound to DPV-encoded UL27 and inhibited its expression, suggesting that UL27 was the target gene of dev-miR-D28-3p. Finally, we investigated the role of UL27 in DPV replication and found the overexpression of UL27 increased viral copies, viral titers, and viral protein expressions; whereas the opposite results appear when knockdown of UL27. Our findings illustrated a novel mechanism that DPV regulated itself replication via dev-miR-D28-3p, paving the way for exploring the role of DPV-encoded miRNAs.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"297 ","pages":"Article 110202"},"PeriodicalIF":2.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SLC25A12 inhibits Japanese encephalitis virus replication by interacting with the NS1 and enhancing the type I interferon response SLC25A12 通过与 NS1 相互作用并增强 I 型干扰素反应来抑制日本脑炎病毒的复制

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-29 DOI: 10.1016/j.vetmic.2024.110199

You-qin Yin , Le-le Liu , Yu-ting Jiang , Jin-chao Xing , Wen-bao Qi , Li-hong Huang

{"title":"SLC25A12 inhibits Japanese encephalitis virus replication by interacting with the NS1 and enhancing the type I interferon response","authors":"You-qin Yin , Le-le Liu , Yu-ting Jiang , Jin-chao Xing , Wen-bao Qi , Li-hong Huang","doi":"10.1016/j.vetmic.2024.110199","DOIUrl":"10.1016/j.vetmic.2024.110199","url":null,"abstract":"<div>Japanese encephalitis virus (JEV) is a mosquito-borne, zoonotic orthoflavivirus causing human encephalitis and reproductive disorders in pigs. Cell-intrinsic antiviral restriction factors are the first line of defense that prevent a virus from establishing a productive infection, while the molecular mechanism of the virus-host interaction is still not fully understood. Our in vitro experiments demonstrated that the Solute Carrier Family 25 Member 12 (SLC25A12) interacted with the JEV nonstructural protein 1 (NS1) and inhibited JEV replication. Furthermore, we showed that knockdown or knockout of SLC25A12 promoted JEV replication, while overexpression of SLC25A12 repressed viral replication. Finally, we demonstrated that SLC25A12 increased IRF7 mRNA levels, which promoted IFN-β expression and subsequently induced antiviral effects. Collectively, our study revealed that SLC25A12 interacted with NS1, inhibiting viral RNA synthesis and transcription and enhancing type I interferon induction for antiviral effects.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"297 ","pages":"Article 110199"},"PeriodicalIF":2.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strenuous expression of porcine epidemic diarrhea virus ORF3 protein suggests host resistance 猪流行性腹泻病毒 ORF3 蛋白的剧烈表达表明宿主具有抵抗力

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-26 DOI: 10.1016/j.vetmic.2024.110193

Antony Ndirangu Kamau , Jung-Eun Yu , Eusi-Soon Park , Jae-Rang Rho , Eui-Ju Hong , Hyun-Jin Shin

{"title":"Strenuous expression of porcine epidemic diarrhea virus ORF3 protein suggests host resistance","authors":"Antony Ndirangu Kamau , Jung-Eun Yu , Eusi-Soon Park , Jae-Rang Rho , Eui-Ju Hong , Hyun-Jin Shin","doi":"10.1016/j.vetmic.2024.110193","DOIUrl":"10.1016/j.vetmic.2024.110193","url":null,"abstract":"<div>Porcine epidemic diarrhea virus is attenuated upon adaptation to cell culture. Exclusively genomic mutations have been traced to the ORF3 gene of the laboratory strains. Previous attempts to express the protein were unsuccessful. We sought to express the ORF3 protein in both mammalian and bacteria cells as a prerequisite for investigation of the protein’s role. For prokaryotic expression, two vector systems, pET28-a(+) and pGEX-4T-1 were constructed and expressed in Escherichia coli cells. For eukaryotic analyses, ORF3/pEGFP-C1 vector constructs were expressed in human embryonic, green monkey kidney and mouse fibrous cells. Intriguingly, there was minimal expression of the ORF3 gene. Following a documented hint that truncated ORF3 revealed higher expression, ORF3 gene was truncated. The simple modular architecture research tool analysis predicted two transmembrane domains between amino acid (aa) 41–63 and aa 76–98. Consequently, we generated two fragments; ORF-N (aa 1–98) inclusive of transmembrane domains and ORF3-C (aa 99–224). These truncated sequences were constructed as the whole gene here referred to as ORF3 wild type (wt). Coomassie blue stained gels revealed bands of ORF3-C expressed as a fusion protein of 17.5 and 39 kDa in pET28-a(+) and pGEX-4T-1 vectors, respectively. In contrast, ORF3-N was not. Additionally, ORF3-N induction decreased total cellular proteins suggesting inhibition of protein synthesis or metabolism. Solubility tests carried out at 30 °C, 25 °C and 18 °C showed that ORF3 formed inclusion bodies. Similar findings were observed in mammalian cells. Noteworthy, morphological distortions appeared in mammalian cells expressing ORF3 protein or its truncated mutants suggesting significance in host viability.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"297 ","pages":"Article 110193"},"PeriodicalIF":2.4,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nanoparticle vaccine based on the VP121–26 and VP2 structural proteins of Senecavirus A induces robust protective immune responses 基于塞内卡病毒 A 的 VP121-26 和 VP2 结构蛋白的纳米颗粒疫苗可诱导强效保护性免疫反应。

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-25 DOI: 10.1016/j.vetmic.2024.110198

Nan Cao , Yamei Li , Huawei Zhang , Xiangzu Liu , Shudan Liu , Mingxing Lu , Zihui Hu , Linxing Tian , Xiangmin Li , Ping Qian

{"title":"A nanoparticle vaccine based on the VP121–26 and VP2 structural proteins of Senecavirus A induces robust protective immune responses","authors":"Nan Cao , Yamei Li , Huawei Zhang , Xiangzu Liu , Shudan Liu , Mingxing Lu , Zihui Hu , Linxing Tian , Xiangmin Li , Ping Qian","doi":"10.1016/j.vetmic.2024.110198","DOIUrl":"10.1016/j.vetmic.2024.110198","url":null,"abstract":"<div>Senecavirus A (SVA) is a causative agent that can cause vesicular disease in swine, which causes a great threat to the swine husbandry in the world. Therefore, it is necessary to develop a vaccine that can effectively prevent the spread of SVA. In this study, we developed a 24-polymeric nano-scaffold using β-annulus peptide from tomato bushy effect virus (TBSV) by coupling this antigen to SVA B cell epitope VP121–26 and VP2 proteins via linkers, respectively. The SVA-based nanoparticle protein of the VP1(B)-β-VP2 was expressed and purified by low-cost prokaryotic system to prepare a SVA nanoparticle vaccine. The immunological protective effect of SVA nanoparticle vaccine was evaluated in mouse and swine models, respectively. The results suggested that both mice and swine could induce high levels SVA neutralizing antibodies and IgG antibodies after two doses immunization. In addition, the swine challenge protection experiment showed that the protection rate of immune SVA nanoparticle vaccine and SVA inactivated vaccine both were 80 %, while the negative control had no protection effect. It demonstrated that SVA nanoparticle vaccine effectively prevented SVA infection in swine. In summary, the preparation of SVA vaccine by using β-annulus peptide is a promising candidate vaccine for prevent SVA transmission, and provides a new idea for the development of novel SVA vaccines.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"296 ","pages":"Article 110198"},"PeriodicalIF":2.4,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging resistance to florfenicol in Actinobacillus pleuropneumoniae isolates on two Italian pig farms 意大利两个养猪场分离的胸膜肺炎放线杆菌对氟苯尼考的新抗药性。

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-25 DOI: 10.1016/j.vetmic.2024.110186

A. Brenciani , S.N. Coccitto , L. Cucco , M. Ustulin , E. Albini , M. Paniccià , D. Vio , M. Cinthi , E. Giovanetti , F.R. Massacci , C.F. Magistrali

{"title":"Emerging resistance to florfenicol in Actinobacillus pleuropneumoniae isolates on two Italian pig farms","authors":"A. Brenciani , S.N. Coccitto , L. Cucco , M. Ustulin , E. Albini , M. Paniccià , D. Vio , M. Cinthi , E. Giovanetti , F.R. Massacci , C.F. Magistrali","doi":"10.1016/j.vetmic.2024.110186","DOIUrl":"10.1016/j.vetmic.2024.110186","url":null,"abstract":"<div>Actinobacillus pleuropneumoniae is responsible for porcine pleuropneumonia, a highly contagious lung infection. The control of this respiratory disease remains heavily reliant on antibiotics, with phenicols being one of the primary classes of antibiotics used in pig farming. In the present study, we describe three isolates (B2278, B2176 and B2177) of A. pleuropneumoniae resistant to florfenicol attributed to the presence of the floR gene, which were obtained from two pig farms in Italy. Florfenicol susceptibility tests indicated that B2176 exhibited an intermediate susceptibility profile, while B2177 and B2278 were resistant. All three isolates belonged to serovar 6 and tested positive for the presence of the floR gene. Whole genome sequencing analysis revealed that isolates B2176, B2177 and B2278 harbored genes encoding the toxins ApxII and ApxIII, characteristic of strains with moderate virulence. Moreover, phylogenetic analysis demonstrated that these isolates were closely related, with single nucleotide polymorphisms (SNPs) ranging from 8 to 19. The floR gene was located on a novel 5588 bp plasmid, designated as pAp-floR. BLASTN analysis showed that the pAp-floR plasmid had high nucleotide identity (99 %) and coverage (60 %) with the pMVSCS1 plasmid (5621 bp) from Mannheimia varigena MVSCS1 of porcine origin. Additionally, at least under laboratory conditions, pAp-floR was stably maintained even in the absence of direct selective pressure, suggesting that it does not impose a fitness cost. Our study underscores the necessity of monitoring the spread of florfenicol-resistant A. pleuropneumoniae isolates in the coming years.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"296 ","pages":"Article 110186"},"PeriodicalIF":2.4,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoprotective efficacy of 3 Klebsiella pneumoniae type I fimbriae proteins in a murine model 3 种肺炎克雷伯氏菌 I 型缘膜蛋白在小鼠模型中的免疫保护功效

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-24 DOI: 10.1016/j.vetmic.2024.110197

Xiaofang Tong , Zhongming Cao , Siying Cheng , Baoling Zhang , Xiaoping Li , John P. Kastelic , Chuang Xu , Bo Han , Jian Gao

{"title":"Immunoprotective efficacy of 3 Klebsiella pneumoniae type I fimbriae proteins in a murine model","authors":"Xiaofang Tong , Zhongming Cao , Siying Cheng , Baoling Zhang , Xiaoping Li , John P. Kastelic , Chuang Xu , Bo Han , Jian Gao","doi":"10.1016/j.vetmic.2024.110197","DOIUrl":"10.1016/j.vetmic.2024.110197","url":null,"abstract":"<div>Klebsiella pneumoniae is a primary cause of clinical mastitis in dairy cows, with prevention being crucial, as treatments often fail due to antimicrobial resistance. Recent studies identified type I fimbrial antigens of K. pneumoniae as promising vaccine candidates, but there are limited research data. In this study, 3 fimbriae genes (fimA, fimC and fimG) were cloned and recombinantly expressed in Escherichia coli and their protective efficacy against K. pneumoniae evaluated in a mouse model. All 3 recombinant fimbriae proteins elicited strong humoral immune responses in mice, significantly increasing IgG, IgG1 and IgG2a. Notably, using a model of mice challenged with an intraperitoneal injection of bacteria, FimG significantly reduced bacterial loads in the spleen and lung, whereas FimA and FimC had limited protection for these organs. Either active or passive immunization with FimG produced substantial protective effects in mice challenged with K. pneumoniae LD100; in contrast, the mortality rate in the FimA-immunized group was similar to that of the control group, whereas FimC had weak protection. We concluded that the FimG recombinant protein vaccine had a favorable protective effect, with potential for immunization against K. pneumoniae mastitis.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"297 ","pages":"Article 110197"},"PeriodicalIF":2.4,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case-control study to identify the causative agents of ophthalmia and conjunctivitis in goats in Savannakhet province of Lao PDR 病例对照研究，旨在确定老挝人民民主共和国沙湾拿吉省山羊眼炎和结膜炎的致病因子。

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.vetmic.2024.110195

P.P. Jayasekara , C. Jenkins , P.F. Gerber , L. Olmo , T. Xaikhue , W. Theppangna , S.W. Walkden-Brown

{"title":"Case-control study to identify the causative agents of ophthalmia and conjunctivitis in goats in Savannakhet province of Lao PDR","authors":"P.P. Jayasekara , C. Jenkins , P.F. Gerber , L. Olmo , T. Xaikhue , W. Theppangna , S.W. Walkden-Brown","doi":"10.1016/j.vetmic.2024.110195","DOIUrl":"10.1016/j.vetmic.2024.110195","url":null,"abstract":"<div>Pinkeye is a highly contagious disease of goats with different aetiologies. Surveys in Lao PDR have identified eye lesions typical of pinkeye as a common condition, however, this has not been confirmed diagnostically, and the responsible pathogens have not been identified. A matched case-control study was implemented in 70 goat holdings from Savannakhet province, Lao PDR, to detect agents causing pinkeye and conduct phylogenetic analysis of the identified pathogens. Fifty eye swabs from goats with infected eyes (cases) and 50 paired samples from unaffected cohorts (controls) were collected from 25 holdings. Samples were tested using quantitative PCR assays targeting known pinkeye pathogens at the genus and species levels. The prevalence of pathogens in case and control goats was as follows: Mycoplasma conjunctivae (94% and 74% respectively, P = 0.006, OR = 5.5), Chlamydia pecorum (4%, 10%), Moraxella ovis (30%, 30%), Moraxella bovis (0%, 0%) and Moraxella bovoculi (0%, 0%). M. conjunctivae was present in a high proportion of goats in both groups revealing that Lao goats are carriers of M. conjunctivae. However, the mean log10 genome copy number/µL of DNA extract was significantly higher in case goats than control goats (P < 0.05). Thus, M. conjunctivae is likely the principal causative agent of pinkeye in Lao goats with carrier status converting to clinical infection following corneal damage or other causative factors. M. conjunctivae detected in samples from different goats and districts showed low genetic diversity. Identifying the causes of pinkeye in Lao goats will assist in designing appropriate treatment and control strategies.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"296 ","pages":"Article 110195"},"PeriodicalIF":2.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0378113524002177/pdfft?md5=7ec02f669cc89790be69b7b25172260f&pid=1-s2.0-S0378113524002177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A phosphodiesterase CpdB in Yersinia pseudotuberculosis degrades CDNs to inhibit innate immune response 耶尔森氏菌中的一种磷酸二酯酶 CpdB 能降解 CDNs，从而抑制先天性免疫反应

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.vetmic.2024.110194

Xiao Wang , Xinwei Hao , Yuqing Yang, Siyu Jia, Yating Chen, Wenguang Yang, Yi Luo, Zhen Xie, Yanchao Gu, Yuxuan Wu, Fuhua Zhang, Mengyuan Li, Yao Wang, Xihui Shen, Lei Xu

{"title":"A phosphodiesterase CpdB in Yersinia pseudotuberculosis degrades CDNs to inhibit innate immune response","authors":"Xiao Wang , Xinwei Hao , Yuqing Yang, Siyu Jia, Yating Chen, Wenguang Yang, Yi Luo, Zhen Xie, Yanchao Gu, Yuxuan Wu, Fuhua Zhang, Mengyuan Li, Yao Wang, Xihui Shen, Lei Xu","doi":"10.1016/j.vetmic.2024.110194","DOIUrl":"10.1016/j.vetmic.2024.110194","url":null,"abstract":"<div>Yersinia pseudotuberculosis (Yptb) is a pathogenic gram-negative bacterium that can colonize the intestines of different animals. Its infection leads to the activation of the host’s innate immunity. Both host and bacterial-derived cyclic dinucleotides (CDNs) could activate the innate immune response of host cells. In bacteria, CDNs like c-di-AMP, c-di-GMP, or 3′3'-cGAMP can be hydrolyzed by different hydrolases. Recent studies showed that the degradation of those second messengers helps the pathogen evade immune detection. In this study, we identified a hydrolase, YPK_3776, namely CpdB in Yptb. CpdB is predicted to bind bacterial-derived c-di-AMP, c-di-GMP, 3′3'-cGAMP and host-derived 2′3'-cGAMP. Surprisingly, by using high-performance liquid chromatography (HPLC), we found that CpdB could only degrade bacterial-derived CDNs but not host-derived 2′3'-cGAMP. In addition, CpdB has 2′3'-cNMP activity. Consistently, the Yptb mutant lacking the cpdB gene exhibited a higher level of intracellular c-di-GMP. Furthermore, the ∆cpdB mutant elicited stronger innate immune responses during Yptb infection in macrophages, suggesting CpdB enables Yptb to evade host immune surveillance. Furthermore, CpdB inhibited the Yptb-induced innate immune response in a STING-dependent manner. Finally, we showed the ∆cpdB infection in mice model exhibited in lower bacterial burden, as compared to wild-type strain infection, indicating CpdB is important for bacterial survival in the host. Together, we identified a cyclic dinucleotide hydrolase CpdB in Yptb that could degrade bacterial-derived CDNs which help the pathogen to evade immune detection via the STING pathway.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"297 ","pages":"Article 110194"},"PeriodicalIF":2.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141843025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclophilin A promotes porcine deltacoronavirus replication by regulating autophagy via the Ras/AKT/NF-κB pathway 嗜环蛋白 A 通过 Ras/AKT/NF-κB 途径调节自噬作用，从而促进猪 deltacoronavirus 的复制

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.vetmic.2024.110190

Yousheng Peng , Chenchen Li , Liping Zhang , Ruiming Yu , Yonglu Wang , Li Pan , Huichen Guo , Yanming Wei , Xinsheng Liu

{"title":"Cyclophilin A promotes porcine deltacoronavirus replication by regulating autophagy via the Ras/AKT/NF-κB pathway","authors":"Yousheng Peng , Chenchen Li , Liping Zhang , Ruiming Yu , Yonglu Wang , Li Pan , Huichen Guo , Yanming Wei , Xinsheng Liu","doi":"10.1016/j.vetmic.2024.110190","DOIUrl":"10.1016/j.vetmic.2024.110190","url":null,"abstract":"<div>Porcine deltacoronavirus (PDCoV) is an important enteric coronavirus that has caused major worldwide economic losses in the pig industry. Previous studies have shown that cyclophilin A (CypA), a key player in aetiological agent infection, is involved in regulating viral infection. However, the role of CypA during PDCoV replication remains unknown. Therefore, in this study, the role of CypA in PDCoV replication was determined. The results demonstrated that PDCoV infection increased CypA expression in LLC-PK1 cells. CypA overexpression substantially promoted PDCoV replication. Proteomic analysis was subsequently used to assess changes in total protein expression levels after CypA overexpression. Gene Ontology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to further determine the mechanisms by which CypA affects viral replication. Proteomic analysis revealed that CypA protein overexpression significantly upregulated 75 differentially expressed proteins and significantly downregulated 172 differentially expressed proteins. The differentially expressed proteins were involved mainly in autophagy and activation of the host innate immune pathway. Subsequent experimental results revealed that the CypA protein promoted viral replication by reducing the levels of natural immune cytokines and mitigated the inhibitory effect of chloroquine (CQ) on viral replication. Further investigation revealed that CypA could activate the Ras/AKT/NF-κB pathway, mediate autophagy signalling and promote PDCoV replication. In summary, the findings of this study may help elucidate the role of CypA in PDCoV replication.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"297 ","pages":"Article 110190"},"PeriodicalIF":2.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of antibiotic resistance in Gram-negative bacilli in wild and exotic healthy birds in Brazil: A warning sign 巴西野生和外来健康鸟类革兰氏阴性杆菌的抗生素耐药性分析：警示信号。

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.vetmic.2024.110196

Carolina Aparecida Ramos , Joseane Cristina Ferreira , Anelise Stella Ballaben , Rafael Antonio Casarin Penha Filho , Ana Lúcia da Costa Darini

{"title":"Analysis of antibiotic resistance in Gram-negative bacilli in wild and exotic healthy birds in Brazil: A warning sign","authors":"Carolina Aparecida Ramos , Joseane Cristina Ferreira , Anelise Stella Ballaben , Rafael Antonio Casarin Penha Filho , Ana Lúcia da Costa Darini","doi":"10.1016/j.vetmic.2024.110196","DOIUrl":"10.1016/j.vetmic.2024.110196","url":null,"abstract":"<div>Bacterial antibiotic resistance is a public health problem affecting humans and animals. This study focuses on identifying Gram-negative bacilli (GNB) (MALDI-TOF MS and Klebsiella MALDI TypeR) resistant to antimicrobials in freshly emitted feces of healthy captive and rescued wild birds from a zoo in Brazil. Birds from the zoo and rescued from sixteen different orders were investigated. Resistant bacteria from feces were selected (MacConkey agar with 2 μg/mL cefotaxime). Genomic similarity and plasmid were investigated by Pulsed-Field Gel Electrophoresis of XbaI fragments (XbaI-PFGE) and S1-PFGE. Polymerase Chain Reaction (PCR) was performed to search for beta-lactamase genes. From 80 birds included, 26 from the zoo (50 %) and 18 rescued wild birds (64 %) presented cefotaxime-resistant GNB. E. coli and Klebsiella spp were the most prevalent species. Among 65 isolates from the zoo and rescued wild birds, 75 % were considered multidrug-resistant (MDR). The majority of the isolates were extended-spectrum beta-lactamases (ESBL) producing and resistant to enrofloxacin. blaCTX-M-GROUP-1, blaTEM, and blaSHV were the most detected genes, and blaKPC was detected in K. pneumoniae complex. According to genomic similarity results, some identical profiles were found in birds with no known contact among the zoo or rescued birds. Several isolates carried one to three plasmids (15–350 kb). The presence of multidrug-resistant (MDR) isolates from healthy captive and wild birds brings novel data on the dissemination of these elements to the environment.</div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"296 ","pages":"Article 110196"},"PeriodicalIF":2.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0