Veterinary microbiology最新文献

{"title":"Tracking of single virus: Dual fluorescent labeling of pseudorabies virus for observing entry and replication in the N2a cells","authors":"Mingzhi Li ,&nbsp;Li Pan ,&nbsp;Caoyuan Ma ,&nbsp;Hongxia Wu ,&nbsp;Guangtao Xiang ,&nbsp;Lian-Feng Li ,&nbsp;Tao Wang ,&nbsp;Rui Luo ,&nbsp;Yongfeng Li ,&nbsp;Di Liu ,&nbsp;Huanjie Zhai ,&nbsp;Moon Assad ,&nbsp;Xin Song ,&nbsp;Yanjin Wang ,&nbsp;Franck Gallardo ,&nbsp;Hua-Ji Qiu ,&nbsp;Yuan Sun","doi":"10.1016/j.vetmic.2025.110503","DOIUrl":"10.1016/j.vetmic.2025.110503","url":null,"abstract":"<div><div>Pseudorabies virus (PRV) is a neurotropic herpesvirus. It is not easy to be track the whole replication progress of PRV, especially the nascent viral genome in the host cells. In this study, we developed a dual-fluorescence-labeled PRV (rPRV-Anchor3-mCherry) with the viral genome and the envelope protein gM labeled by ANCHOR DNA labeling system and mCherry, respectively. Through single-virus tracking of rPRV-Anchor3-mCherry, we observed that PRV invaded mouse neuroblastoma Neuro-2a cells via both endocytosis and plasma membrane fusion pathway. During the replication stage, parental and progeny viral genome of rPRV-Anchor3-mCherry in the cell nuclei could be visible, and viral nucleocapsid appeared more specifically than traditional capsid protein labeled PRV particles (rPRV-VP26-EGFP). We found that numerous progeny viral particles were produced in the nuclear, causing the nucleus membrane to break using three-dimensional (3D) live-cell imaging and electron microscopy. Moreover, our findings confirmed that simultaneously targeting of the <em>UL9</em> and <em>UL54</em> genes using a CRISPR-Cas9 system led to the complete inhibition PRV replication. rPRV-Anchor3-mCherry can be used to research multiple steps of the viral cycle.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110503"},"PeriodicalIF":2.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VP4-Specific IgA level as a correlate of neutralizing antibody and fecal shedding of porcine rotavirus infection","authors":"Sufen Li ,&nbsp;Xianyu Bian ,&nbsp;Jianxin Wang ,&nbsp;Dandan Wang ,&nbsp;Jinzhu Zhou ,&nbsp;Jiapeng Song ,&nbsp;Wei Wang ,&nbsp;Nan Han ,&nbsp;Junming Zhou ,&nbsp;Yunchuan Li ,&nbsp;Ran Tao ,&nbsp;Xuejiao Zhu ,&nbsp;Baochao Fan ,&nbsp;Hailong Dong ,&nbsp;Xuehan Zhang ,&nbsp;Bin Li","doi":"10.1016/j.vetmic.2025.110501","DOIUrl":"10.1016/j.vetmic.2025.110501","url":null,"abstract":"<div><div>Rotavirus (RV) causes diarrhea in children, infants, and young animals globally, with public health implications. Porcine rotavirus (PoRV) leads to economic losses in swine farming. Neutralizing antibodies (NAb) are vital for protecting piglets from intestinal infections. However, which serum and mucosal markers correlate with NAbs against PoRV and relate to post-infection fecal shedding remains unclear, crucial for pathogen-specific detection. We used indirect ELISA to measure IgG/IgA in sera, sIgA in colostrum from recovered pigs, and feces from diarrheal piglets against VP4*, VP7*, VP6, and NSP4*. Analyses showed specific IgA/sIgA levels correlated better with NAb titers than IgG. Among them, VP4*-specific IgA/sIgA had the highest positive correlation with NAb titers in sera (R = 0.848, P &lt; 0.0001) and colostrum (R = 0.865, P &lt; 0.0001). Also, VP4*-specific IgA/sIgA in sera (R= −0.446, P &lt; 0.001) and feces (R= −0.497, P &lt; 0.0001) had the strongest inverse relationship with viral RNA load. Piglet passive protection tests confirmed VP4*-specific IgA's high neutralizing capacity, highly correlated with NAb titers (R = 0.858, P &lt; 0.0001), reducing viral shedding. In conclusion, mucosal IgA/sIgA responses to VP4 are important for PoRV diagnosis assays and vaccine efficacy evaluation.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110501"},"PeriodicalIF":2.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What’s up ducks? – Antimicrobial resistance of Escherichia coli isolated from duck farm environment in Poland extended with genomic characteristics of cephalosporin-resistant strains","authors":"Magdalena Skarżyńska ,&nbsp;Magdalena Zając ,&nbsp;Renata Kwit ,&nbsp;Anna Lalak ,&nbsp;Aleksandra Śmiałowska-Węglińska ,&nbsp;Paulina Pasim ,&nbsp;Ewelina Skrzypiec ,&nbsp;Weronika Koza ,&nbsp;Dominika Wojdat ,&nbsp;Emilia Mikos-Wojewoda ,&nbsp;Dominika Pastuszka ,&nbsp;Arkadiusz Bomba ,&nbsp;Dariusz Wasyl","doi":"10.1016/j.vetmic.2025.110492","DOIUrl":"10.1016/j.vetmic.2025.110492","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) in food-producing animals is a source of concern as it may pose a risk to public health. Studies of ducks in this area seem to be scarce. Thus, we aimed to evaluate the AMR occurrence in <em>Escherichia coli</em> from a duck farm environment in Poland. We applied official AMR monitoring methods to investigate AMR in <em>E</em>. <em>coli</em> isolated from boot swabs collected at 306 duck farms in Poland. The samples were screened for indicator, cephalosporin-, carbapenem- and colistin- resistant <em>E</em>. <em>coli</em>. Isolates were tested for antimicrobial susceptibility using the microbroth dilution method and interpreted with epidemiological cut-off values. Whole Genome Sequencing (WGS) of cephalosporin-resistant strains enabled an in-depth insight into specific resistance mechanisms, mobile genetic elements and phylogeny of strains. A total of 340 strains were isolated. The percentage of indicator <em>E</em>. <em>coli</em> equaled 89.9 %, while 19.3 % were obtained through selective screening for <em>E</em>. <em>coli</em> resistant to cephalosporins. Six were recovered on colistin-supplemented MacConkey agar. Among indicator <em>E</em>. <em>coli</em> 81.1 % were resistant and ciprofloxacin, ampicillin, tetracycline and nalidixic acid resistance were the most frequent, followed by folate-path inhibitors. Within the group of strains from cephalosporin resistance screening: 76.3 % exhibited ESBL-, 20.3 % AmpC-, and 3.4 % showed both ESBL- and AmpC- phenotypes. WGS of those strains revealed numerous AMR determinants, not only genes corresponding to mentioned phenotypes but also determinants encoding resistance to other medically important antimicrobials. Our study reveals that <em>E</em>. <em>coli</em> from duck farm environment constitute a reservoir of AMR determinants including those of public health concern.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110492"},"PeriodicalIF":2.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant protein PntA coordinates with OmpW to resist oxidant stress in Actinobacillus pleuropneumoniae","authors":"Weiyao Han ,&nbsp;Qiuhong Zhang ,&nbsp;Zhen Luo ,&nbsp;Hao Tang ,&nbsp;Xiabing Chen ,&nbsp;Qi Huang ,&nbsp;Rui Zhou ,&nbsp;Lu Li","doi":"10.1016/j.vetmic.2025.110500","DOIUrl":"10.1016/j.vetmic.2025.110500","url":null,"abstract":"<div><div>Bacteria have evolved various strategies to combat oxidative stress caused by reactive oxygen species (ROS). Outer membrane proteins including OmpW play multiple roles in bacterial physiology, stress responses and virulence. In this study, the OmpW protein of <em>Actinobacillus pleuropneumoniae</em>, an important porcine respiratory tract pathogen, was found to contribute to virulence but concurrently to reduce resistance to oxidative stress. An <em>ompW</em> deletion (Δ<em>ompW</em>) showed attenuation in mice, and decreased adherence to pig tracheal epithelial cells and resistance to hyperosmotic stress, compared to the wild-type (WT) strain. However, the Δ<em>ompW</em> strain exhibited increased resistance to H₂O₂, enhanced survival ability within macrophages, and lower intracellular ROS level. OmpW may serve as a H₂O₂ channel. Further study showed that exposure to H₂O₂ significantly suppressed <em>ompW</em> transcription in the WT strain. Overexpression of these two proteins in WT and Δ<em>ompW</em> increased the antioxidative properties of the bacteria. Furthermore, by construction of the double gene mutant Δ<em>ompW</em>Δ<em>pntA</em>, it was found that PntA could reverse the effects of OmpW on the bacterial survivability and intracellular ROS level after H₂O₂ treatment. Therefore, by interacting with OmpW, PntA alleviated the increased oxidative stress sensitivity caused by OmpW. These results suggest a mechanism whereby antioxidant proteins collaborate with OMPs to protect bacteria from oxidative stress.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110500"},"PeriodicalIF":2.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteome profiling of the lung tissues of the mice co-infected with H9N2 influenza A virus and Pseudomonas aeruginosa","authors":"Sun Ying-Li ,&nbsp;Fan Hua ,&nbsp;Li Wen-qian ,&nbsp;Li Dan ,&nbsp;Bai Ke-fei ,&nbsp;Dong Lu-jiao ,&nbsp;Zhang Hui-ning ,&nbsp;Li Jian-liang ,&nbsp;Xie Zhi-jing","doi":"10.1016/j.vetmic.2025.110499","DOIUrl":"10.1016/j.vetmic.2025.110499","url":null,"abstract":"<div><div>H9N2 Influenza A virus (IAV) can contribute to <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) superinfection, causing severe pneumonia. But the underlying mechanisms remain unclear. In this study, to investigate molecular ecology of the lung tissues co-infected with H9N2 IAV and <em>P. aeruginosa</em>, the mouse models were developed to analyze proteome of the lung tissues. As a result, the differential proteins of the lungs sampled from the mice with single H9N2 IAV infection, single <em>P. aeruginosa</em> infection and co-infection with H9N2 IAV and <em>P. aeruginosa</em> were related to immune responses, cell proliferation and apoptosis, which were involved in NF-κB pathway, Toll-like signaling pathway, RIG-I signaling pathway and JAK-STAT signaling pathway. It implied that the different infection combinations of H9N2 IAV and <em>P. aeruginosa</em> influenced the protein expression levels. Based on the proteomics assays, the three proteins, NLRX1, ISG15 and IRF9, were screened to be further characterized using the in vitro MH-S cell models with H9N2 IAV and <em>P. aeruginosa</em> co-infection. The findings demonstrated that NLRX1, IRF9 and ISG15 might play the important roles in immune response against H9N2 IAV and <em>P. aeruginosa</em> co-infection and are potential targets for the development of drugs to prevent and treat the diseases.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110499"},"PeriodicalIF":2.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Misidentification of Raoultella spp. (R. terrigena, R. planticola) and Klebsiella spp. (K. variicola, K. grimontii) as Klebsiella pneumoniae: Retrospective study of a necropsy-associated bacterial collection from horses","authors":"Francois Gravey ,&nbsp;Corinne Sévin ,&nbsp;Bénédicte Langlois ,&nbsp;Karine Maillard ,&nbsp;Nathalie Foucher ,&nbsp;Fabien Duquesne ,&nbsp;Albertine Léon ,&nbsp;Simon Le Hello ,&nbsp;Sandrine Petry","doi":"10.1016/j.vetmic.2025.110497","DOIUrl":"10.1016/j.vetmic.2025.110497","url":null,"abstract":"<div><div>Misidentifications as <em>Klebsiella pneumoniae</em> were observed during a French retrospective study of a necropsy-associated <em>K. pneumoniae</em> bacterial collection from horses. Accordingly, the present study aimed to further characterise the 12 <em>Raoultella</em> spp. and <em>Klebsiella</em> spp. strains involved in these misidentifications. The strains were identified and characterised using the Api 20E system, <em>K. pneumoniae</em> PCR detection, matrix-assisted laser desorption/ionisation time-of-flight mass spectroscopy and whole-genome sequencing. Antimicrobial susceptibilities were tested by the disc diffusion method with a panel of 40 antibiotics. Thus, misidentifications as <em>K. pneumoniae</em> mainly concerned <em>Raoultella</em> spp. (<em>R. terrigena</em>, <em>R. planticola</em>) rather than <em>Klebsiella</em> spp. (<em>K. variicola</em>, <em>K. grimontii</em>), with a dominance of <em>R. terrigena</em>. Among the 12 strains, only <em>K. grimontii</em> was multi-drug resistant and none were considered hypervirulent. MALDI-TOF was sufficient to avoid misidentification but commercial spectra databases should be expanded with <em>K. grimontii</em> and <em>R. terrigena</em> reference spectra to improve identification accuracy. This is probably (i) the first report of <em>K. grimontii</em> and <em>R. planticola</em> isolations from horses and (ii) the second report of <em>R. terrigena</em> and <em>K. variicola</em> isolations from horses.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110497"},"PeriodicalIF":2.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and antimicrobial resistance profiles of Clostridium perfringens isolated from pets in China","authors":"Mingjin Fang ,&nbsp;Yuan Yuan ,&nbsp;Xin Tian ,&nbsp;Ying Liu ,&nbsp;Ke Wu ,&nbsp;Yanyan Zhu ,&nbsp;Likun Zhang ,&nbsp;Edward M. Fox ,&nbsp;Ruichao Li ,&nbsp;Li Bai ,&nbsp;Menghan Li ,&nbsp;Guerrino Macori ,&nbsp;Xinglong Wang ,&nbsp;Dongyang Ye ,&nbsp;Zengqi Yang ,&nbsp;Rong Zhang ,&nbsp;Juan Wang","doi":"10.1016/j.vetmic.2025.110490","DOIUrl":"10.1016/j.vetmic.2025.110490","url":null,"abstract":"<div><div><em>Clostridium perfringens</em> is a notable pathogen causing diarrhea in domestic animals. However, data on this pathogen’s prevalence and genomic characteristics in pets are limited. We collected 300 fecal samples from companion animals across two cities in China and isolated 150 strains for genomic sequencing and antimicrobial susceptibility testing (AST). Our findings showed a high prevalence of two key virulence genes (VGs), <em>pfoA</em> (77.33 %, 116/150) and <em>cpb2</em> (60.67 %, 91/150). Moreover, for the first time in China, we identified a strain carrying <em>netF</em>. The analysis of AST and genomic antimicrobial resistance genes (ARGs) highlighted the resistance patterns of pet-derived <em>C. perfringens</em> strains in China. Particularly, we observed a high resistance rate to erythromycin, primarily associated with <em>erm(Q)</em>, which was found in 88.67 % (133/150) of all isolates. This resistance rate was higher than that reported in previous studies. Genetic context analysis identified a novel plasmid group harboring the <em>erm(Q)</em> gene. In summary, this study revealed the antibiotic resistance and genomic characteristics of <em>C. perfringens</em> strains derived from pets in China, providing a reference for the prevention of related diseases and further research. Notably, these findings underscore the need for continuous monitoring of resistance trends, particularly concerning the spread of <em>erm(Q)</em>, to lessen the impact of antimicrobial resistance in veterinary medicine.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110490"},"PeriodicalIF":2.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The important role of TLR2/MyD88/JNK in regulating the pathogenesis and inflammation induced by pseudorabies virus in mice","authors":"Jingyi Niu ,&nbsp;Gang Li ,&nbsp;Yiyu Liu,&nbsp;Chao Wan,&nbsp;Yixuan Li,&nbsp;Yu Dai,&nbsp;Haixia Hu,&nbsp;Lianci Peng,&nbsp;Rendong Fang,&nbsp;Chao Ye","doi":"10.1016/j.vetmic.2025.110496","DOIUrl":"10.1016/j.vetmic.2025.110496","url":null,"abstract":"<div><div>The host innate immune response plays a critical role in regulating and controlling viral infections by releasing inflammatory cytokines. Pseudorabies virus (PRV), a swine alphaherpesvirus, can cause severe encephalitis in piglets and various non-natural hosts. Previous studies demonstrated that PRV infection induced the significant elevation of pro-inflammatory cytokines levels and lethal inflammatory response in the mouse model. However, the underlying mechanisms responsible for activation and production of pro-inflammatory cytokines during PRV infection remain to be fully elucidated. In this study, we confirmed that PRV induced significant inflammatory response in C57BL/6 mice during its acute infection. Furthermore, TLR2/MyD88 axis was shown to be associated with the pathogenesis of PRV in mice. Specifically, TLR2/MyD88 axis was required for PRV-induced activation of NF-κB pathway and the subsequent pro-inflammatory cytokines expression. Meanwhile, MAPK/JNK and PI3K/Akt signaling pathways were also activated by TLR2/MyD88 axis and involved in regulating pro-inflammatory cytokines expression induced by PRV infection, respectively. Notably, administration of the JNK inhibitor (SP600125) could reduce clinical symptoms, alleviate pathological damage and prolong survival time of mice infected by PRV. Overall, this study strengthens our understanding upon the mechanism of host inflammatory response induced by PRV, and suggests that JNK signaling may act as a therapeutic target in controlling of PRV infection.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110496"},"PeriodicalIF":2.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics of Brucella abortus isolated from African Buffalo in Kruger National Park: New perspectives on wildlife-cattle disease dynamics","authors":"Carlo Andrea Cossu ,&nbsp;Giuliano Garofolo ,&nbsp;Anna Janowicz ,&nbsp;Fabrizio De Massis ,&nbsp;Jeanette Wentzel ,&nbsp;Maphuti Betty Ledwaba ,&nbsp;Claude Sabeta ,&nbsp;Lin-Mari De Klerk ,&nbsp;Jacques Godfroid ,&nbsp;Gilles Vergnaud ,&nbsp;Henriette van Heerden","doi":"10.1016/j.vetmic.2025.110493","DOIUrl":"10.1016/j.vetmic.2025.110493","url":null,"abstract":"<div><div>In South Africa, <em>Brucella abortus</em> biovar 1 is the primary cause of bovine brucellosis, significantly impacting cattle production and trade. Serological studies have revealed brucellosis in African wildlife, complicating control efforts due to limited epidemiological data. In 1977, <em>B. abortus</em> biovar 1 was isolated from an African buffalo fetus in Kruger National Park (KNP), raising speculation that buffalo may serve as reservoir hosts. This study investigated <em>Brucella</em> spp. in free-ranging buffalo in KNP using serological, molecular, and bacteriological methods. <em>Brucella abortus</em> bv 1 was isolated from lymph nodes and spleens of three sub-adult buffalo in 2022, marking the first documented recurrence in 50 years. Phylogenomic analyses revealed connections between buffalo isolates and cattle strains from South Africa and South America, suggesting spillover and shared origins from Europe. Further genomic and epidemiological surveillance is required to clarify the role of buffalo as reservoir hosts for brucellosis.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110493"},"PeriodicalIF":2.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JEV NS1' protein enhances cell-to-cell viral spread by inducing the formation of tunneling nanotubes","authors":"Ziyu Cao,&nbsp;Shengda Xie,&nbsp;Xingmiao Yang,&nbsp;Ning Wei,&nbsp;Yundi Zhao,&nbsp;Mengyuan Sun,&nbsp;Xinxin Lin,&nbsp;Miaolei Shi,&nbsp;Ruibing Cao","doi":"10.1016/j.vetmic.2025.110494","DOIUrl":"10.1016/j.vetmic.2025.110494","url":null,"abstract":"<div><div>Japanese encephalitis virus (JEV), a mosquito-borne virus, can infect various host cells. However, the efficiency with which JEV spreads from cell to cell remains unclear. This study demonstrates that JEV infection can induce the formation of tunneling nanotubes (TNTs), which is mediated by the viral NS1' protein. Further investigations revealed that NS1' protein induces TNTs formation by interacting with PAK1. The establishment of TNTs facilitates the transport of JEV virions and the NS1' protein, thereby evading neutralization by antibodies. In conclusion, our study elucidates the mechanism through which JEV induces the formation of TNTs in host cells and highlights a novel pathway for JEV intercellular spread.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110494"},"PeriodicalIF":2.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}