Veterinary microbiology最新文献_第9页

Contamination of alpine pastures by Mycobacterium avium subsp. paratuberculosis: Evaluation of cattle and red deer contribution through environmental fecal samples 禽分枝杆菌亚种对高山牧场的污染。副结核：通过环境粪便样本评估牛和马鹿的贡献

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-08-05 DOI: 10.1016/j.vetmic.2025.110661

Chiara Garbarino , Matteo Nava , Anita Filippi , Alessandro Forti , Simone Russo , Filippo Barsi , Alessandro Bianchi , Joel Filipe , Norma Arrigoni , Luca Pedrotti , Luca Corlatti , Matteo Ricchi , Camilla Luzzago

{"title":"Contamination of alpine pastures by Mycobacterium avium subsp. paratuberculosis: Evaluation of cattle and red deer contribution through environmental fecal samples","authors":"Chiara Garbarino , Matteo Nava , Anita Filippi , Alessandro Forti , Simone Russo , Filippo Barsi , Alessandro Bianchi , Joel Filipe , Norma Arrigoni , Luca Pedrotti , Luca Corlatti , Matteo Ricchi , Camilla Luzzago","doi":"10.1016/j.vetmic.2025.110661","DOIUrl":"10.1016/j.vetmic.2025.110661","url":null,"abstract":"<div><div>Paratuberculosis is a chronic enteritis of ruminants (<em>Bovidae</em>, <em>Cervidae</em>) caused by <em>Mycobacterium avium</em> subsp<em>. paratuberculosis</em> (MAP). The prevalence of shedding animals, together with fecal MAP loads, is critical to the biocontamination of the environment. Surveillance for paratuberculosis has been introduced within the frame of European Health Law (Regulation EU 2016/429) both in livestock and wild ruminants. Semi-extensive and extensive livestock production may determine exposure to MAP infection and intraspecies and interspecies transmission on pasture. In the present study MAP prevalence and viable shedding in red deer and cattle were assessed through environmental fecal sampling over two summer seasons in the Alps. A low but widespread percentage of MAP-positive feces in both species was observed, indicating a small proportion of MAP grazing shedders. Consequently, the risk for MAP exposure on alpine pastures seems to be low. The highest contamination was reported mainly in overlapping areas for both species. The present study supports the utility and reliability of environmental fecal sampling as a non-invasive sampling approach to assess paratuberculosis shedding in grazing animals.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"309 ","pages":"Article 110661"},"PeriodicalIF":2.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlations between virulence gene profiles and other genetic factors of Pasteurella multocida strains isolated from various host species 多杀性巴氏杆菌不同宿主菌株毒力基因谱与其他遗传因素的相关性研究

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-31 DOI: 10.1016/j.vetmic.2025.110657

Krisztina Pintér , Marianna Domán , Enikő Wehmann , László Makrai , Hubert Gantelet , Tibor Magyar

{"title":"Correlations between virulence gene profiles and other genetic factors of Pasteurella multocida strains isolated from various host species","authors":"Krisztina Pintér , Marianna Domán , Enikő Wehmann , László Makrai , Hubert Gantelet , Tibor Magyar","doi":"10.1016/j.vetmic.2025.110657","DOIUrl":"10.1016/j.vetmic.2025.110657","url":null,"abstract":"<div><div><em>Pasteurella multocida</em> has a high genotypic variability, which allows it to cause a wide variety of diseases with huge economic losses worldwide. In this study, whole genome sequencing (WGS) of 66 <em>P. multocida</em> strains isolated from different host species was performed. By including an additional 23 strains selected from the NCBI database, genotypic characteristics of a total of 89 isolates were analysed. Sequence analysis revealed the strains' capsular serogroup, LPS genotype, multi-host (MH) and Rural Industries Research and Development Corporation (RIRDC) multilocus sequence typing (MLST). The most frequent serotype was A:L3, followed by F:3 and D:L6. The pattern of 10 virulence associated genes (<em>f</em>imA, <em>hgb</em>A, <em>hgb</em>B, <em>hsf</em>-1, <em>hsf</em>-2, <em>nan</em>H, <em>pfh</em>A, <em>ptf</em>A, <em>tad</em>D, <em>tbp</em>A) classified the strains into 10 new virulence gene profiles (VGPs), adding to the 13 VGPs described previously to provide a total of 23 VGPs. Some strains harboured multiple point mutations within <em>tad</em>D, that prevented primer binding during PCR, causing false negative results. New primers were designed to validate WGS findings and eliminate primer-binding mismatches, enhancing future diagnostic accuracy. A correlation was observed between the <em>omp</em>A gene sequences, MH and RIRDC STs of the strains and their VGP group. It was also observed that <em>tbp</em>A is only present in strains of ruminant origin, and further correlations were found between other genotypic characteristics of the strains and the occurrence of virulence genes.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110657"},"PeriodicalIF":2.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144772559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial prophylaxis in dogs undergoing catheter-based cardiac interventions and pacemaker implantation – A survey 接受导管心脏干预和起搏器植入的狗的抗菌预防-一项调查

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-29 DOI: 10.1016/j.vetmic.2025.110656

Iris L. Blok , Mark Rishniw , J. Scott Weese , Viktor Szatmári

{"title":"Antimicrobial prophylaxis in dogs undergoing catheter-based cardiac interventions and pacemaker implantation – A survey","authors":"Iris L. Blok , Mark Rishniw , J. Scott Weese , Viktor Szatmári","doi":"10.1016/j.vetmic.2025.110656","DOIUrl":"10.1016/j.vetmic.2025.110656","url":null,"abstract":"<div><div>Guidelines for prophylactic antimicrobial use for catheter-based cardiac interventions and pacemaker implementation in dogs do not exist. Optimizing antimicrobial use by rationalizing indications and the duration of administration is essential against the ever-growing threat of antimicrobial resistance. The aim of our study was to reveal the current habits of prophylactic antimicrobial use among veterinarians who perform transvascular cardiac interventions in dogs. A digital survey was offered via the mailing list of an international veterinary cardiology-interest group. The 141 respondents reported large variations in protocols (e.g., 47 different prophylaxis protocols for pacemaker implantation). Protocols differed on administration (yes/no), drug, and the route and duration of administration (only intravenous, or intravenous followed by oral antimicrobials). The median duration of antimicrobial course was 7 days (range: 0–21 days). Antimicrobial prophylaxis was administered by 89 % of 141 respondents for occlusion of patent ductus arteriosus, 76 % of 139 respondents for balloon valvuloplasty of pulmonary valve stenosis, 93 % of 56 respondents for transpulmonary stenting, and 96 % of 136 respondents for transvenous pacemaker implantation. The existence of institutional guidelines was reported by 30 % of the respondents, although these also showed substantial variation (e.g., 27 guidelines from 40 institutions for pacemaker implantation). Veterinarians use prophylactic antimicrobials more often and for longer periods for cardiac interventions than their human counterparts. Antibiotic prophylaxis shows little consistency between veterinarians, and does not appear to be supported by evidence of necessity. This warrants re-evaluation of use, and creation of guidelines describing rational use of antimicrobials in interventional cardiac procedures.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110656"},"PeriodicalIF":2.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Varicellovirus bovinealpha 1 UL42 targets host IRF3 to inhibit type I interferon β production 牛痘病毒1 UL42靶向宿主IRF3抑制I型干扰素β的产生

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-27 DOI: 10.1016/j.vetmic.2025.110654

Xinhuan Yin , Qian Du , Dingwen Feng , Donglai Song , Hanpei Zhang , Yong Huang , Dewen Tong

{"title":"Varicellovirus bovinealpha 1 UL42 targets host IRF3 to inhibit type I interferon β production","authors":"Xinhuan Yin , Qian Du , Dingwen Feng , Donglai Song , Hanpei Zhang , Yong Huang , Dewen Tong","doi":"10.1016/j.vetmic.2025.110654","DOIUrl":"10.1016/j.vetmic.2025.110654","url":null,"abstract":"<div><div>Varicellovirus bovinealpha 1 (formerly bovine alphaherpesvirus type 1, BoAHV-1), a significant pathogen in cattle with substantial economic impacts, establishes lifelong latency and employs multiple immune evasion strategies. In this study, we identified the BoAHV-1 nonstructural protein UL42 as a modulator of the cGAS-STING pathway that suppresses type I interferon β (IFN-β) production. Besides, we find that bovine IRF3 only contains four conserved serine/threonine residues at the C-terminus and its mutant named as IRF3–4D resembles IRF3–5D of other species to retain nuclear localization capability and transcriptional activity. Mechanistically, BoAHV-1 UL42 directly interacts with IRF3 and disrupts the binding of IRF3 to IFN-β promoter without affecting IRF3 phosphorylation or nuclear translocation, thereby inhibiting IFN-β transcription and downstream interferon-stimulated gene (ISG) expression. Further analysis revealed that residues Arg282 and Arg283 of UL42 are critical for its immunosuppressive activity. Together, our results identify BoAHV-1 UL42 as a critical protein that specifically disrupts IRF3-mediated IFN-β production signaling to subvert host antiviral responses.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110654"},"PeriodicalIF":2.7,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic divergence of GAPDH in Mycoplasma synoviae: Comparative insights from MS-H and TS4 strains 滑支原体GAPDH的酶分化：MS-H和TS4菌株的比较研究

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-27 DOI: 10.1016/j.vetmic.2025.110653

Sahar Zare, Marc S. Marenda, Sara M. Klose, Amir H. Noormohammadi

{"title":"Enzymatic divergence of GAPDH in Mycoplasma synoviae: Comparative insights from MS-H and TS4 strains","authors":"Sahar Zare, Marc S. Marenda, Sara M. Klose, Amir H. Noormohammadi","doi":"10.1016/j.vetmic.2025.110653","DOIUrl":"10.1016/j.vetmic.2025.110653","url":null,"abstract":"<div><div><em>Mycoplasma synoviae</em> causes respiratory infections and lameness in poultry, resulting in significant production losses. It carries two gene copies encoding the glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The <em>M. synoviae</em> strain MS-H, a live attenuated vaccine derived from the virulent isolate 86079/7NS (7NS), carries a distinct amino acid substitution per copy of <em>gapdh</em>. A field re-isolate of MS-H with increased pathogenicity, TS4, possesses a wild-type reversion in one of these copies, suggesting an association with virulence, but the functionality of each GAPDH variant is unclear. Here, a colorimetric assay was used to quantify the enzymatic activities of recombinant GAPDH variants VR, AK, and AR (each representing residues 185 and 306, respectively) expressed in <em>Escherichia coli</em>. The GAPDH variants were tested individually and in combination to represent MS-H (VR + AK), 7NS (AR), and TS4 (VR + AR), and the results were placed in contrast to those of crude cell lysates from 7NS, MS-H, and TS4 cultures. The AK and AR variants exhibited comparable enzymatic activity, whereas VR showed no detectable activity. Protein mixtures simulating MS-H and TS4 exhibited activity levels equivalent to AR and AK. The enzymatic activity in TS4 crude lysate was lower than in MS-H and 7NS. <em>In-silico</em> analysis revealed that the Ala185Val substitution in VR (which abolished its enzymatic activity) affects the NAD<sup>+</sup> binding site, while the Arg306Lys in AK is close to the glyceraldehyde-3-phosphate binding site does not affect the NAD<sup>+</sup> binding site. This study provides insights into how amino acid substitutions in GAPDH influence enzymatic function and highlights their complex role in <em>M. synoviae</em> pathogenesis.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110653"},"PeriodicalIF":2.7,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An attractive vaccine candidate based on AP205 VLPs fusing with a receptor-binding domain of a newly emerged porcine deltacoronavirus strain in China 基于AP205 VLPs与中国新出现的猪三角冠状病毒株受体结合域融合的有吸引力的候选疫苗

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-27 DOI: 10.1016/j.vetmic.2025.110655

Xinyue Chang , Zhenyuan Zhang , Yilian Chen , Minghui Zhang , Fei Fei , Chenhao Ma , Zhiying Wang , Fazhi Xu , Lin Li , Xinxin Shan , Liang Li , Pei Sun , Xuelan Liu

{"title":"An attractive vaccine candidate based on AP205 VLPs fusing with a receptor-binding domain of a newly emerged porcine deltacoronavirus strain in China","authors":"Xinyue Chang , Zhenyuan Zhang , Yilian Chen , Minghui Zhang , Fei Fei , Chenhao Ma , Zhiying Wang , Fazhi Xu , Lin Li , Xinxin Shan , Liang Li , Pei Sun , Xuelan Liu","doi":"10.1016/j.vetmic.2025.110655","DOIUrl":"10.1016/j.vetmic.2025.110655","url":null,"abstract":"<div><div>Emerging swine enteric coronaviruses (SeCoV) pose significant threats to global pork security, with zoonotic porcine deltacoronavirus (PDCoV) demonstrating cross-species transmission potential. Here, we identified a new PDCoV strain, CHN/GD/2023, which was isolated from porcine intestinal epithelium, showing phylogenetic clustering with China-endemic variants through viral spike (S) protein evolutionary tracing. A vaccine candidate for PDCoV was produced by genetically fusing the receptor-binding domain (RBD) of CHN/GD/2023 to the C-terminus of the AP205 gene. The purified AP205-RBD successfully displayed RBD on and exhibited a spherical nanoparticle structure packaging nucleic acid inside particles. Furthermore, AP205-RBD generate high level of RBD-specific IgG antibodies, neutralizing PDCoV, and concurrently enhancing both CD4<sup>+</sup> and CD8<sup>+</sup> T-cell responses compared to controls, which has the potential to be produced at large scale for immunization programs.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110655"},"PeriodicalIF":2.7,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the effect of vaccination on the impact of BTV-3 in Dutch sheep flocks in 2024 评估2024年荷兰羊群BTV-3疫苗接种效果

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-24 DOI: 10.1016/j.vetmic.2025.110652

E. Dijkstra , I.M.G.A. Santman-Berends , A. Stegeman , K.M.J.A. van den Brink , G. van Schaik , R. van den Brom

{"title":"Evaluating the effect of vaccination on the impact of BTV-3 in Dutch sheep flocks in 2024","authors":"E. Dijkstra , I.M.G.A. Santman-Berends , A. Stegeman , K.M.J.A. van den Brink , G. van Schaik , R. van den Brom","doi":"10.1016/j.vetmic.2025.110652","DOIUrl":"10.1016/j.vetmic.2025.110652","url":null,"abstract":"<div><div>Bluetongue virus serotype 3 (BTV-3) emerged in the Netherlands in 2023 and re-emerged in 2024, causing a substantial impact on the sheep population. In 2024, inactivated BTV-3 vaccines became available, yet clinical cases and excess mortality were still reported, including among vaccinated flocks. This study aimed to quantify the effect of BTV-3 vaccination on excess mortality in sheep and lambs during the 2024 epidemic, using data from twenty veterinary practises, and to evaluate whether a protective effect could be detected at the national level.</div><div>Vaccination records from participating veterinary practices were linked to national census and mortality data. Multivariable regression models were used to estimate the association between vaccination status and mortality, adjusting for farm type, flock size, region, season, and prior BTV-3 exposure. Results indicated that inactivated BTV-3 vaccination was associated with a significant reduction in excess mortality in both adult sheep and lambs. However, mortality during the epidemic remained significantly higher than pre-epidemic baselines, regardless of vaccination status. Analysis of national data supported the protective effect of vaccination. Nevertheless, over 65,000 excess sheep deaths during the 2024 epidemic were recorded, although the impact per farm was lower in 2024 compared to 2023. Incomplete vaccine registration and potential misclassification of vaccination status, may have led to a slight under-or over estimation of vaccine effectiveness. In conclusion, inactivated BTV-3 vaccines reduced mortality but did not fully prevent disease or halt transmission. For future outbreaks, improved national systems for recording vaccination data are required to accurately assess vaccination effectiveness and support outbreak response. Reliable census and mortality data remain essential for effective outbreak monitoring and evaluation of disease control strategies.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"309 ","pages":"Article 110652"},"PeriodicalIF":2.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic shifts in gut microbiota composition of cats following oral infection with the virulent FIPV strain rQS-79 and treatment with GS-441524 猫口腔感染FIPV毒株rQS-79和用GS-441524治疗后肠道菌群组成的动态变化

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-24 DOI: 10.1016/j.vetmic.2025.110646

Ashenafi Assefa Gebremariam , Muhammad Muazzam Iqbal , Donghan Wang , Wang Peng Peng , Yuejun Shi , Guiqing Peng

{"title":"Dynamic shifts in gut microbiota composition of cats following oral infection with the virulent FIPV strain rQS-79 and treatment with GS-441524","authors":"Ashenafi Assefa Gebremariam , Muhammad Muazzam Iqbal , Donghan Wang , Wang Peng Peng , Yuejun Shi , Guiqing Peng","doi":"10.1016/j.vetmic.2025.110646","DOIUrl":"10.1016/j.vetmic.2025.110646","url":null,"abstract":"<div><div>Feline infectious peritonitis (FIP) is a fatal disease caused by a mutant strain of feline coronavirus (FCoV), and its progression is closely related to gut microbiota and immune response. This study aimed to investigate the changes in the fecal microbiota composition of cats experimentally infected with FIPV, with and without antiviral treatment. Fifteen healthy cats were orally inoculated with the rQS-79 FIPV strain and treated with the antiviral drug GS-441524. Fecal samples were analyzed using 16S rRNA-based next-generation sequencing to assess the microbial diversity and composition. These findings demonstrated that FIPV infection and its treatment led to changes in the quantity and structure of the gut microbiota at the phylum and species levels. Although no significant taxa were linked to a specific group, Firmicutes was the predominant phylum, followed by Actinobacteria and Bacteroidota. Infected cats showed decreased levels of beneficial bacteria, such as <em>Bifidobacterium</em> and <em>Lactobacillus</em> (p < 0.05), along with increased levels of pathogenic <em>Clostridium</em> and <em>Escherichia</em>. Treatment with GS-441524 reduced the abundance of certain pathogenic (<em>Peptoclostridium spp.</em>) and beneficial (<em>Bifidobacterium spp.</em>) bacteria in the gut. These findings highlight that both viral infection and antiviral treatment disrupt the gut microbial balance. Thus, an integrated approach combining antiviral drugs, probiotics, and dietary modulation may offer a more effective strategy for restoring microbiota homeostasis and improving the outcomes of FIP and similar viral infections in cats.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110646"},"PeriodicalIF":2.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction to veterinary microbiology special issue featuring advance in swine viral disease research 《兽医微生物学导论》特刊，介绍猪病毒性疾病的研究进展。

IF 2.7 2区农林科学

Veterinary microbiology Pub Date : 2025-07-23 DOI: 10.1016/j.vetmic.2025.110649

Ying Fang, Scott Kenney, Pablo E. Pineyro-Pineiro, Raymond Rowland, Kimberly VanderWaal

引用次数: 0

Dual-mode delivery of canine parvovirus type 2c VP2 protein in recombinant Lactobacillus plantarum: Surface display and intracellular expression systems 重组植物乳杆菌中犬细小病毒2c型VP2蛋白的双模式传递：表面显示和细胞内表达系统

IF 2.4 2区农林科学

Veterinary microbiology Pub Date : 2025-07-20 DOI: 10.1016/j.vetmic.2025.110651

Zhe Xu , Huaiyue He , Dan Wang , Mengmeng Xiao , Jinman Li , Yulin Hou , Yangyi Chen , Xuan Zu , Jingtao Hu , Guilian Yang , Yanlong Jiang , Wentao Yang , Haibin Huang , Chunwei Shi , Yan Zeng , Nan Wang , Xin Cao , Jianzhong Wang , Chunfeng Wang

{"title":"Dual-mode delivery of canine parvovirus type 2c VP2 protein in recombinant Lactobacillus plantarum: Surface display and intracellular expression systems","authors":"Zhe Xu , Huaiyue He , Dan Wang , Mengmeng Xiao , Jinman Li , Yulin Hou , Yangyi Chen , Xuan Zu , Jingtao Hu , Guilian Yang , Yanlong Jiang , Wentao Yang , Haibin Huang , Chunwei Shi , Yan Zeng , Nan Wang , Xin Cao , Jianzhong Wang , Chunfeng Wang","doi":"10.1016/j.vetmic.2025.110651","DOIUrl":"10.1016/j.vetmic.2025.110651","url":null,"abstract":"<div><div>Canine parvovirus type 2 (CPV-2) causes an acute and highly transmissible disease characterized by severe hemorrhagic enteritis and myocarditis, ultimately resulting in the death of dogs and wild carnivores. At present, although vaccines have been developed for CPV-2, there are no specific and effective vaccines available for prevention of CPV-2c infection. To develop mucosal vaccines against CPV-2c, we constructed recombinant <em>Lactobacillus plantarum</em> (<em>L. plantarum</em>) expressing the VP2 antigen of CPV-2c. We amplified the VP2 gene from a CPV-2c subtype strain and subsequently constructed recombinant <em>L. plantarum</em> NC8 strains that express the VP2 and pgsA’-VP2 proteins, respectively. This work has shown that recombinant <em>L. plantarum</em> strains NC8/pSIP409-VP2 and NC8/pSIP409-pgsA’-VP2 can effectively activate dendritic cells maturation markers (CD80, CD86, and MHC-Ⅱ) while inducing robust IgG and IFN-γ responses in both mice and dogs. Furthermore, recombinant <em>L. plantarum</em> elevated sIgA and IgG concentrations and augmented B220⁺IgA⁺ cell populations. The data indicated that the recombinant <em>L. plantarum</em> developed in this work can significantly enhance the host immunity as an oral vaccination and may contribute to the prevention and control of canine parvovirus infection in dogs.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"308 ","pages":"Article 110651"},"PeriodicalIF":2.4,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0