Veterinary microbiology最新文献

{"title":"Misidentification of Raoultella spp. (R. terrigena, R. planticola) and Klebsiella spp. (K. variicola, K. grimontii) as Klebsiella pneumoniae: Retrospective study of a necropsy-associated bacterial collection from horses","authors":"Francois Gravey ,&nbsp;Corinne Sévin ,&nbsp;Bénédicte Langlois ,&nbsp;Karine Maillard ,&nbsp;Nathalie Foucher ,&nbsp;Fabien Duquesne ,&nbsp;Albertine Léon ,&nbsp;Simon Le Hello ,&nbsp;Sandrine Petry","doi":"10.1016/j.vetmic.2025.110497","DOIUrl":"10.1016/j.vetmic.2025.110497","url":null,"abstract":"<div><div>Misidentifications as <em>Klebsiella pneumoniae</em> were observed during a French retrospective study of a necropsy-associated <em>K. pneumoniae</em> bacterial collection from horses. Accordingly, the present study aimed to further characterise the 12 <em>Raoultella</em> spp. and <em>Klebsiella</em> spp. strains involved in these misidentifications. The strains were identified and characterised using the Api 20E system, <em>K. pneumoniae</em> PCR detection, matrix-assisted laser desorption/ionisation time-of-flight mass spectroscopy and whole-genome sequencing. Antimicrobial susceptibilities were tested by the disc diffusion method with a panel of 40 antibiotics. Thus, misidentifications as <em>K. pneumoniae</em> mainly concerned <em>Raoultella</em> spp. (<em>R. terrigena</em>, <em>R. planticola</em>) rather than <em>Klebsiella</em> spp. (<em>K. variicola</em>, <em>K. grimontii</em>), with a dominance of <em>R. terrigena</em>. Among the 12 strains, only <em>K. grimontii</em> was multi-drug resistant and none were considered hypervirulent. MALDI-TOF was sufficient to avoid misidentification but commercial spectra databases should be expanded with <em>K. grimontii</em> and <em>R. terrigena</em> reference spectra to improve identification accuracy. This is probably (i) the first report of <em>K. grimontii</em> and <em>R. planticola</em> isolations from horses and (ii) the second report of <em>R. terrigena</em> and <em>K. variicola</em> isolations from horses.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110497"},"PeriodicalIF":2.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and antimicrobial resistance profiles of Clostridium perfringens isolated from pets in China","authors":"Mingjin Fang ,&nbsp;Yuan Yuan ,&nbsp;Xin Tian ,&nbsp;Ying Liu ,&nbsp;Ke Wu ,&nbsp;Yanyan Zhu ,&nbsp;Likun Zhang ,&nbsp;Edward M. Fox ,&nbsp;Ruichao Li ,&nbsp;Li Bai ,&nbsp;Menghan Li ,&nbsp;Guerrino Macori ,&nbsp;Xinglong Wang ,&nbsp;Dongyang Ye ,&nbsp;Zengqi Yang ,&nbsp;Rong Zhang ,&nbsp;Juan Wang","doi":"10.1016/j.vetmic.2025.110490","DOIUrl":"10.1016/j.vetmic.2025.110490","url":null,"abstract":"<div><div><em>Clostridium perfringens</em> is a notable pathogen causing diarrhea in domestic animals. However, data on this pathogen’s prevalence and genomic characteristics in pets are limited. We collected 300 fecal samples from companion animals across two cities in China and isolated 150 strains for genomic sequencing and antimicrobial susceptibility testing (AST). Our findings showed a high prevalence of two key virulence genes (VGs), <em>pfoA</em> (77.33 %, 116/150) and <em>cpb2</em> (60.67 %, 91/150). Moreover, for the first time in China, we identified a strain carrying <em>netF</em>. The analysis of AST and genomic antimicrobial resistance genes (ARGs) highlighted the resistance patterns of pet-derived <em>C. perfringens</em> strains in China. Particularly, we observed a high resistance rate to erythromycin, primarily associated with <em>erm(Q)</em>, which was found in 88.67 % (133/150) of all isolates. This resistance rate was higher than that reported in previous studies. Genetic context analysis identified a novel plasmid group harboring the <em>erm(Q)</em> gene. In summary, this study revealed the antibiotic resistance and genomic characteristics of <em>C. perfringens</em> strains derived from pets in China, providing a reference for the prevention of related diseases and further research. Notably, these findings underscore the need for continuous monitoring of resistance trends, particularly concerning the spread of <em>erm(Q)</em>, to lessen the impact of antimicrobial resistance in veterinary medicine.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110490"},"PeriodicalIF":2.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The important role of TLR2/MyD88/JNK in regulating the pathogenesis and inflammation induced by pseudorabies virus in mice","authors":"Jingyi Niu ,&nbsp;Gang Li ,&nbsp;Yiyu Liu,&nbsp;Chao Wan,&nbsp;Yixuan Li,&nbsp;Yu Dai,&nbsp;Haixia Hu,&nbsp;Lianci Peng,&nbsp;Rendong Fang,&nbsp;Chao Ye","doi":"10.1016/j.vetmic.2025.110496","DOIUrl":"10.1016/j.vetmic.2025.110496","url":null,"abstract":"<div><div>The host innate immune response plays a critical role in regulating and controlling viral infections by releasing inflammatory cytokines. Pseudorabies virus (PRV), a swine alphaherpesvirus, can cause severe encephalitis in piglets and various non-natural hosts. Previous studies demonstrated that PRV infection induced the significant elevation of pro-inflammatory cytokines levels and lethal inflammatory response in the mouse model. However, the underlying mechanisms responsible for activation and production of pro-inflammatory cytokines during PRV infection remain to be fully elucidated. In this study, we confirmed that PRV induced significant inflammatory response in C57BL/6 mice during its acute infection. Furthermore, TLR2/MyD88 axis was shown to be associated with the pathogenesis of PRV in mice. Specifically, TLR2/MyD88 axis was required for PRV-induced activation of NF-κB pathway and the subsequent pro-inflammatory cytokines expression. Meanwhile, MAPK/JNK and PI3K/Akt signaling pathways were also activated by TLR2/MyD88 axis and involved in regulating pro-inflammatory cytokines expression induced by PRV infection, respectively. Notably, administration of the JNK inhibitor (SP600125) could reduce clinical symptoms, alleviate pathological damage and prolong survival time of mice infected by PRV. Overall, this study strengthens our understanding upon the mechanism of host inflammatory response induced by PRV, and suggests that JNK signaling may act as a therapeutic target in controlling of PRV infection.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110496"},"PeriodicalIF":2.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics of Brucella abortus isolated from African Buffalo in Kruger National Park: New perspectives on wildlife-cattle disease dynamics","authors":"Carlo Andrea Cossu ,&nbsp;Giuliano Garofolo ,&nbsp;Anna Janowicz ,&nbsp;Fabrizio De Massis ,&nbsp;Jeanette Wentzel ,&nbsp;Maphuti Betty Ledwaba ,&nbsp;Claude Sabeta ,&nbsp;Lin-Mari De Klerk ,&nbsp;Jacques Godfroid ,&nbsp;Gilles Vergnaud ,&nbsp;Henriette van Heerden","doi":"10.1016/j.vetmic.2025.110493","DOIUrl":"10.1016/j.vetmic.2025.110493","url":null,"abstract":"<div><div>In South Africa, <em>Brucella abortus</em> biovar 1 is the primary cause of bovine brucellosis, significantly impacting cattle production and trade. Serological studies have revealed brucellosis in African wildlife, complicating control efforts due to limited epidemiological data. In 1977, <em>B. abortus</em> biovar 1 was isolated from an African buffalo fetus in Kruger National Park (KNP), raising speculation that buffalo may serve as reservoir hosts. This study investigated <em>Brucella</em> spp. in free-ranging buffalo in KNP using serological, molecular, and bacteriological methods. <em>Brucella abortus</em> bv 1 was isolated from lymph nodes and spleens of three sub-adult buffalo in 2022, marking the first documented recurrence in 50 years. Phylogenomic analyses revealed connections between buffalo isolates and cattle strains from South Africa and South America, suggesting spillover and shared origins from Europe. Further genomic and epidemiological surveillance is required to clarify the role of buffalo as reservoir hosts for brucellosis.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110493"},"PeriodicalIF":2.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JEV NS1' protein enhances cell-to-cell viral spread by inducing the formation of tunneling nanotubes","authors":"Ziyu Cao,&nbsp;Shengda Xie,&nbsp;Xingmiao Yang,&nbsp;Ning Wei,&nbsp;Yundi Zhao,&nbsp;Mengyuan Sun,&nbsp;Xinxin Lin,&nbsp;Miaolei Shi,&nbsp;Ruibing Cao","doi":"10.1016/j.vetmic.2025.110494","DOIUrl":"10.1016/j.vetmic.2025.110494","url":null,"abstract":"<div><div>Japanese encephalitis virus (JEV), a mosquito-borne virus, can infect various host cells. However, the efficiency with which JEV spreads from cell to cell remains unclear. This study demonstrates that JEV infection can induce the formation of tunneling nanotubes (TNTs), which is mediated by the viral NS1' protein. Further investigations revealed that NS1' protein induces TNTs formation by interacting with PAK1. The establishment of TNTs facilitates the transport of JEV virions and the NS1' protein, thereby evading neutralization by antibodies. In conclusion, our study elucidates the mechanism through which JEV induces the formation of TNTs in host cells and highlights a novel pathway for JEV intercellular spread.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110494"},"PeriodicalIF":2.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S5092 inhibit ASFV infection by targeting the pS273R protease activity in vitro","authors":"Chuanxia Liu ,&nbsp;Tingting Li ,&nbsp;Tao Huang ,&nbsp;Gaihong Zhao ,&nbsp;Xiao Wang ,&nbsp;Jiangnan Li ,&nbsp;Li Huang ,&nbsp;Zhaoxia Zhang ,&nbsp;Jun Zheng ,&nbsp;Changjiang Weng","doi":"10.1016/j.vetmic.2025.110473","DOIUrl":"10.1016/j.vetmic.2025.110473","url":null,"abstract":"<div><div>African Swine Fever (ASF) is a highly infectious viral disease caused by African swine fever virus (ASFV). Currently, there is no effective vaccine, and it is urgent to discover antiviral agents to deal with the epidemic. ASFV pS273R is the only known cysteine protease that catalyzes the maturation of the polyprotein precursors pp220 and pp62 through proteolytic cleavage, a critical step in ASFV assembly. In this study, we performed a high-throughput screening of an FDA-approved drug library to identify several compounds that target the active site of the ASFV pS273R enzyme via molecular docking. We initially identified 778 molecules based on the “Drug-like Five Principles”, with S5092 emerging as the most promising candidate. S5092 is a prodrug that can be metabolized into Fenbendazole (FBZ) and Oxfendazole (OFZ). In exogenous cleavage experiments, S5092, FBZ, and OFZ significantly inhibited the cleavage of the viral polyprotein pp220 and pp62, as well as the host antiviral protein gasdermin D (GSDMD) by pS273R, indicating that these compounds inhibit the enzymatic activity of pS273R. Importantly, S5092 and its metabolites reduced the level of ASFV infection, as evidenced by the detection of viral genomic copies and viral titer. Collectively, our findings indicate that S5092 suppresses ASFV infection by inhibiting the enzyme activity of pS273R, a multifunction protein, positioning it as a novel and specific drug candidate for the prevention and control of ASF.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110473"},"PeriodicalIF":2.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-epitope vaccines Xlc and Ddc against Glaesserella parasuis infection in mice","authors":"Lu Dai ,&nbsp;Jiajia Wan ,&nbsp;Rui Zhang ,&nbsp;Tingting Xie ,&nbsp;Yizhen Jia ,&nbsp;Zhichao Lu ,&nbsp;Fuxian Zhang ,&nbsp;Wenting Ke ,&nbsp;Feng Liu ,&nbsp;Liancheng Lei","doi":"10.1016/j.vetmic.2025.110491","DOIUrl":"10.1016/j.vetmic.2025.110491","url":null,"abstract":"<div><div><em>Glaesserella parasuis</em> (synonym <em>Haemophilus parasuis</em>) is the pathogenic agent of Glässer’s disease and causes huge economic losses in the world’s swine industry. <em>Glaesserella parasuis</em> (<em>G. parasuis</em>) can be divided into 15 serotypes, and the cross-protection effect of existing vaccines is not satisfactory. Therefore, the development of a vaccine to prevent multiple serotypes of <em>G. parasuis</em> infection is of great significance for the prevention and treatment of Glässer’s disease, but still faces many difficulties. In this study, the B-cell, CTL and Th cell epitopes of CtdB, CtbC, OppA, TbpB, HxuC, D15, Omp2 and Omp5 proteins were predicted by bioinformatics method, and multi-epitope proteins Xlc and Ddc were obtained by concatenating epitopes through linkers. After immunization with Xlc and Ddc, the levels of antibodies, IL-4, and IFN-γ in mice were significantly increased. The protective rates of Xlc+Ddc immunized mice against <em>G. parasuis</em> serotypes 4, 5, and 10 were 62.5 %, 75 %, and 87.5 %, respectively, which were higher than those of Xlc (37.5 %, 62.5 %, and 87.5 %) and Ddc (75 %, 25 %, and 50 %). Overall, the combination of multi-epitope proteins Xlc and Ddc had good immunogenicity and strong cross-protection against <em>G. parasuis</em> serotypes 4, 5, and 10. These results indicated that multi-epitope proteins Xlc and Ddc can serve as candidate subunit vaccines against <em>G. parasuis</em> infection.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110491"},"PeriodicalIF":2.4,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological and serological insights into Lagovirus diseases dynamics in the European brown hare (Lepus europaeus): A nine-year longitudinal study","authors":"Josep Estruch ,&nbsp;Patrizia Cavadini ,&nbsp;Antonio Lavazza ,&nbsp;Lorenzo Capucci ,&nbsp;Joana Abrantes ,&nbsp;Ana M. Lopes ,&nbsp;Tereza Almeida ,&nbsp;Aleksija Neimanis ,&nbsp;Santiago Lavín ,&nbsp;Carlos Rouco ,&nbsp;Emmanuel Serrano ,&nbsp;Roser Velarde","doi":"10.1016/j.vetmic.2025.110478","DOIUrl":"10.1016/j.vetmic.2025.110478","url":null,"abstract":"<div><div>The European brown hare syndrome virus (EBHSV; GII.1) and rabbit haemorrhagic disease virus 2 (RHDV2; GI.2) are pathogenic lagoviruses affecting the European brown hare (<em>Lepus europaeus</em>). EBHSV/GII.1 causes periodic epidemics, while RHDV2/GI.2 infections emerge from spillover events in areas where hares are sympatric with European rabbits (<em>Oryctolagus cuniculus</em>). In the northeast of the Iberian Peninsula, the overlap of these species provides a unique opportunity to investigate how the epidemiology of these viruses correlates with disease course. We analysed the presence of lagoviruses in 113 European brown hare carcasses recovered in Catalonia (NE Spain) between 2015 and 2024. Animals were necropsied, and tissue and serum samples were collected for histopathology, virological investigation, and serology. Sera from hunted hares apparently healthy (n = 89, 2015–2023) were also included in the study. PCR on liver samples (n = 58) and virological ELISA on positive sera (n = 52) confirmed 28 EBHSV/GII.1 and 24 RHDV2/GI.2 cases. After the first EBHSV/GII.1 detection in 2016, antibody titres decreased progressively until 2020–2021, coinciding with an outbreak. No conclusive seropositivity for RHDV2/GI.2 was observed during the study. Pathology revealed more acute lesions in RHDV2/GI.2-infected hares compared to EBHSV/GII.1. These lesions, resulting in sudden death due to a deficient immune response, may explain this distinct epidemiological scenario. Despite a decade of circulation, RHDV2/GI.2 has not fully adapted to hares. However, ongoing monitoring is essential, as mutations or recombination events could increase its epizootic potential. The co-circulation of both lagoviruses, combined with other co-factors, might jeopardise the viability of European brown hare populations at the southern limit of their range.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110478"},"PeriodicalIF":2.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective investigation of 43 necropsy cases of Tyzzer disease in foals and partial genome sequence of Clostridium piliforme by shotgun metagenomics","authors":"Tirth Uprety ,&nbsp;Melissa Swan ,&nbsp;Laura Kennedy ,&nbsp;Uneeda Bryant ,&nbsp;Lynne Cassone ,&nbsp;Alan Loynachan ,&nbsp;Jennifer Janes ,&nbsp;Molly M. Evely ,&nbsp;Francine C. Calvaruso ,&nbsp;Maria Quick ,&nbsp;Jocelynn Morgan ,&nbsp;Sahar Abdelrazek ,&nbsp;Kevin Lahmers ,&nbsp;Craig Carter ,&nbsp;Erdal Erol","doi":"10.1016/j.vetmic.2025.110489","DOIUrl":"10.1016/j.vetmic.2025.110489","url":null,"abstract":"<div><div><em>Clostridium piliforme</em> is an obligate intracellular filamentous bacterium that causes Tyzzer disease (TD) in many animals. The disease manifests as severe, multifocal necrotizing hepatitis, with a high fatality rate in foals. Through retrospective investigation, we detected <em>C. piliforme</em> in 43 equine necropsy cases from 2012 to 2024. Positive cases were diagnosed from February to July, peaking in May. The age of affected foals ranged from 4 days to 2 months. Histologically, all cases had necrotizing hepatitis with multifocal, coalescing pinpoint, tan or reddish foci. Since only a partial 16S rRNA gene sequence was available for the horse strain of <em>C. piliforme</em>, we used shotgun metagenomics to obtain its genome sequence from the liver of a necropsied foal with TD. The sequences obtained were compared against the NCBI NT/NR database with the highest number of reads and contigs aligning to <em>Clostridium</em> species. A complete 16S rRNA gene was obtained, showing the highest identity to a 16S rRNA gene of the horse strain of <em>C. piliforme</em> (99.05 %), followed by 98.02–96.71 % identities to rabbit and rodent strains of <em>C. piliforme</em>, indicating cross-species variation. Additional identified genes included alveolysin, exo-α-sialidase, flagellar and spore formation/vegetation, providing the first genetic evidence of virulence factors for <em>C. piliforme</em>. Furthermore, presence of genes encoding multidrug export and multidrug resistance proteins suggested <em>C. piliforme</em> could develop resistance to beta-lactams and fluoroquinolones. This study provides the first partial genome sequence of <em>C. piliforme</em> using a shotgun metagenomics hepatic sampling approach on a foal with TD.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110489"},"PeriodicalIF":2.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains","authors":"Teresa Magalí Hoffmann ,&nbsp;Mario Alberto Soria ,&nbsp;Dante Javier Bueno ,&nbsp;Yosef Daniel Huberman ,&nbsp;María Ishida ,&nbsp;Brian Sauders ,&nbsp;Gregory Deiulio ,&nbsp;Alyssa Wiedenmayer Dickey ,&nbsp;José Luis Medina-Santana ,&nbsp;Christian Vinueza-Burgos","doi":"10.1016/j.vetmic.2025.110488","DOIUrl":"10.1016/j.vetmic.2025.110488","url":null,"abstract":"<div><div>The escalating issue of antimicrobial resistance in pathogens transmitted through the food chain poses a significant threat to public health worldwide. <em>Salmonella</em> ser. Enteritidis (SE) remains a predominant cause of foodborne illness. This research aims to elucidate the genetic and antimicrobial resistance profiles of SE strains isolated from Argentine poultry farms. This study used whole genome sequencing to assess the genetic diversity and resistance patterns of 20 SE strains collected from poultry farms in Entre Ríos, Argentina, between January 2020 and February 2021. Phenotypic assays were used to detect resistance to 50 antibiotics across 14 antimicrobial classes. Genes and mutations linked to antimicrobial resistance were pinpointed on both plasmids and chromosomes of the strains. All investigated strains exhibited resistance to nitrofurantoin, and a high percentage showed resistance or intermediate sensitivity to fluoroquinolones. Genomic analysis showed the presence of resistance genes associated with aminoglycosides, β-lactams, and quinolones. The study distinctly categorized the strains into those isolated from broilers and laying hens, highlighting a complex interplay of resistance and diversity within the poultry industry. This shows antimicrobial resistance within foodborne pathogens like SE and the importance of continuous surveillance systems to track the evolution and spread of resistance. The genomic insights from this study not only enhance understanding of the resistance mechanisms but also facilitate tracking the transmission dynamics of SE, which is crucial for developing targeted interventions.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110488"},"PeriodicalIF":2.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}