Veterinary microbiology最新文献

{"title":"Brucella vulpis sp. nov. is attenuated in a BALB/c murine model of infection: Of mice and foxes","authors":"Agustín Rebollada-Merino ,&nbsp;María P. Jiménez de Bagüés ,&nbsp;Maykel A. Arias ,&nbsp;Axel Cloeckaert ,&nbsp;Michel S. Zygmunt","doi":"10.1016/j.vetmic.2025.110583","DOIUrl":"10.1016/j.vetmic.2025.110583","url":null,"abstract":"<div><div>Brucellosis, caused by <em>Brucella</em> spp., is a zoonotic disease of global significance, affecting a wide range of animal hosts. Since the discovery of <em>Brucella</em> spp. in wildlife, novel bacteria belonging to the genus <em>Brucella</em> have been isolated across various terrestrial and aquatic animals. Among them are <em>Brucella vulpis</em> isolates F60^T and F965 cultured from two red foxes (<em>Vulpes vulpes</em>) tissues, classified within the <em>Brucella</em> genus in 2016; however, the behavior and pathogenic potential of <em>B. vulpis</em> in a mouse model have not been investigated yet. In this study, the virulence and serologic response of <em>B. vulpis</em> F60^T and F965 in BALB/c mice were investigated and compared using <em>B. suis</em> 1330, <em>B. microti</em> CCM 4915 and <em>Brucella</em> sp. 83–210 representative of phylogenetically more distant or non-core species, and previously shown to be also virulent in the mouse model. Our findings indicate that <em>B. vulpis</em> exhibits an attenuated infection pattern at 10⁴ CFU, with lower levels of persistence in the spleen and liver than <em>B. suis</em> 1330, <em>B. microti</em> CCM 4915, and <em>Brucella</em> sp. 83–210, and no associated mortality in mice. <em>B. vulpis</em> F60^T and F965 elicited a similar serologic response compared to <em>B. suis</em> 1330, <em>B. microti</em> CCM 4915, and <em>Brucella</em> sp. 83–210, based on an indirect enzyme-linked immunosorbent assay (iELISA). These results indicate that, in a mouse infection model, <em>B. vulpis</em> can replicate and is attenuated, at least for the isolates investigated, compared to the other virulent <em>Brucella</em> spp. studied.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110583"},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of infectious novel goose parvovirus in the skin of duck carcass associated with residual feather burrs","authors":"Xin Du ,&nbsp;Xiaoyan Gong ,&nbsp;Wenjun Dong ,&nbsp;Wanmei Li ,&nbsp;Zhiwei Jiang ,&nbsp;Yufeng Li ,&nbsp;Wei Zhang ,&nbsp;Guoqiang Zhu ,&nbsp;Jianye Wang","doi":"10.1016/j.vetmic.2025.110578","DOIUrl":"10.1016/j.vetmic.2025.110578","url":null,"abstract":"<div><div>Since the emergence of short beak and dwarfism syndrome (SBDS) among Cherry Valley ducks in China, duck carcasses associated with residual feather burrs have been frequently noticed. Novel goose parvovirus (NGPV) DNAs have been detected in the skins of these carcasses, however, whether NGPV present in these carcass skins is still viable remains unclear. In this study, skin samples were collected from duck carcasses with feather burrs at a local slaughterhouse. These samples were homogenized, and the sterilized homogenates were used to inoculate 9-day-old embryonated Cherry Valley duck eggs for viral isolation. Two NGPV isolates, designated as TX2302 and TX2309, were obtained and passaged in embryonated duck eggs. Their genomes were amplified by PCR, and the complete genome sequences were determined. In the phylogenetic tree constructed based on the coding protein sequences, TX2302 and TX2309 showed a closer genetic relationship with the NGPV strains isolated between 2019 and 2023. Experimental infection of 8-day-old Cherry Valley ducks with TX2302 reproduced clinical feathering disorders. Moreover, NGPV was detected in skins of the infected ducks until they reached 29 days of age. Overall, these results confirm the presence of infectious NGPV in the skin of duck carcass with feather burrs. Moreover, they demonstrate that NGPV infection alone can cause feathering disorders, potentially contributing to the formation of feather burrs in duck carcass.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110578"},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of tick-borne pathogens in grey wolves (Canis lupus) in southwestern Europe","authors":"Susana Remesar ,&nbsp;David Cano-Terriza ,&nbsp;Patrocinio Morrondo ,&nbsp;Álvaro Oleaga ,&nbsp;Barbara Moroni ,&nbsp;Nuno Santos ,&nbsp;Riccardo Orusa ,&nbsp;Lisa Guardone ,&nbsp;Pablo Díaz ,&nbsp;David García-Dios ,&nbsp;Saúl Jiménez-Ruiz ,&nbsp;Joana Ferreira- e-Silva ,&nbsp;Moisés Gonzálvez ,&nbsp;Serena Robetto ,&nbsp;Ignacio García-Bocanegra","doi":"10.1016/j.vetmic.2025.110579","DOIUrl":"10.1016/j.vetmic.2025.110579","url":null,"abstract":"<div><div>In Europe, studies on tick-borne pathogens (TBPs) in wolves are limited, primarily focusing on one population. This study aimed to assess the prevalence of TBPs in grey wolves (<em>Canis lupus</em>) from Spain (n = 129), Italy (n = 113), and Portugal (n = 43) between 2001 and 2023. The presence of <em>Anaplasma</em> spp., <em>Ehrlichia</em> spp., <em>Hepatozoon</em> spp. and piroplasm DNA was analyzed in spleen samples, and risk factors for <em>Hepatozoon canis</em> prevalence were evaluated using a generalized linear model<em>.</em> Overall, 93.3 % (266/285) wolves tested positive to at least one TBP, being <em>H. canis</em> the most prevalent (91.6 %). <em>Babesia canis</em> (1.8 %), <em>Anaplasma phagocytophilum</em> (1.4 %), and <em>Babesia capreoli</em> (0.7 %) were detected in small percentages and <em>Ehrlichia</em> spp. and <em>Theileria</em> spp. were not found. Coinfections involving <em>H. canis</em> + <em>B. canis</em> (1.8 %) as well as <em>A. phagocytophilum</em> + <em>B. capreoli</em> (0.4 %) were detected. <em>Hepatozoon canis</em> prevalence was significantly higher in Spain than in Portugal and it was higher in young compared to subadult individuals. The phylogenetic analysis showed the existence of well-defined clades of <em>H. canis</em>. The high prevalence of <em>H. canis</em> indicates that this pathogen is endemic in wolves from these three countries, whereas the importance of <em>Anaplasma</em>, <em>Ehrlichia</em> and piroplasm species seems to be negligible. Our results show a high nucleotide diversity among the <em>H. canis</em> sequences. In addition, our data reveals that wolves from the study countries are infected with TBPs, which could be of health concern. These findings highlight the importance of performing epidemiological studies in wildlife.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110579"},"PeriodicalIF":2.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PERK-mediated eIF2α phosphorylation suppresses porcine epidemic diarrhea virus replication by attenuating global protein synthesis and inducing IFN-Ⅰ production","authors":"Gan Yang ,&nbsp;Yu zhao ,&nbsp;Haoran Guo ,&nbsp;Pei Zhou ,&nbsp;Ling Dong ,&nbsp;Wentao Li ,&nbsp;Qigai He","doi":"10.1016/j.vetmic.2025.110559","DOIUrl":"10.1016/j.vetmic.2025.110559","url":null,"abstract":"<div><div>Porcine epidemic diarrhea virus (PEDV) is one of the most important porcine pathogens for which no preventive and antiviral treatment measures are available. A pervious study revealed that the unfolded protein response (UPR) induced by endoplasmic reticulum (ER) stress can be utilized to inhibit PEDV replication. Here, we demonstrated that the UPR suppresses the replication of multiple genotypes of PEDV in both Vero and swine testis (ST) cells, primarily through activation of the PERK-eIF2α branch among the three UPR pathways. The PERK-eIF2α pathway inducers CCT020312 and salubrinal efficiently inhibited the replication of multiple genotypes of PEDV in both Vero and ST cells, whereas the inhibitor AMG PERK 44 promoted PEDV replication. Furthermore, we found that PERK-eIF2α arm-mediated inhibition of PEDV replication is caused by phosphorylated eIF2α-induced attenuation of global protein translation. Additionally, phosphorylated eIF2α promotes NF-κB signaling activation and facilitates to the production of IFN-Ⅰ, eliciting innate immunity to suppress viral replication. These data show that PERK-eIF2α pathway dampens the replication of multiple genotypes of PEDV, suggesting that this target may be exploited to develop as a broad-spectrum anti-PEDV drugs.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110559"},"PeriodicalIF":2.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring approaches and advancements in the development and evaluation of multi-epitope subunit vaccines against tick-borne viruses","authors":"Mourad Ben Said ,&nbsp;Myriam Kratou","doi":"10.1016/j.vetmic.2025.110577","DOIUrl":"10.1016/j.vetmic.2025.110577","url":null,"abstract":"<div><div>Tick-borne viruses (TBVs) pose significant public health challenges worldwide, causing severe diseases such as hemorrhagic fevers and encephalitis. Given the limitations of existing therapeutic and preventive measures, the development of effective vaccines represents a critical strategy for controlling TBV-related infections. This review explores recent advancements in the design and evaluation of multi-epitope subunit vaccines targeting key TBVs, including hemorrhagic fever viruses (e.g., Crimean-Congo hemorrhagic fever virus, Severe fever with thrombocytopenia syndrome virus), encephalitis viruses (e.g., West Nile virus, Tick-Borne Encephalitis virus), and emerging or less common TBVs (e.g., Banna virus, Yezo virus). Emphasis is placed on the application of immunoinformatics and reverse vaccinology for the identification of epitopes capable of inducing robust humoral and cellular immune responses. We summarize epitope prediction methodologies, vaccine construct designs, immune simulation results, and molecular docking findings. While current studies demonstrate strong <em>in silico</em> potential, we underline the urgent need for experimental validation to support the clinical development of these vaccine candidates.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"306 ","pages":"Article 110577"},"PeriodicalIF":2.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of pESI-like megaplasmid in Salmonella Infantis from the poultry industry in Korea","authors":"Min Beom Kim ,&nbsp;Young Ju Lee","doi":"10.1016/j.vetmic.2025.110576","DOIUrl":"10.1016/j.vetmic.2025.110576","url":null,"abstract":"<div><div>The plasmid of emerging <em>Salmonella</em> Infantis (pESI) and pESI-like plasmids have been reported as a major cause of the dissemination of <em>S</em>. Infantis worldwide, and these plasmids have also been reported in Korea. In this study, eight pESI or pESI-like plasmids, including two pESI-like plasmids of <em>S.</em> Infantis from the poultry industry in Korea, harbored yersiniabactin operon, toxin/antitoxin systems, and fimbria clusters. Interestingly, various resistance genes were identified in the pESI-like plasmids but not in the pESI plasmid. In particular, <em>dfrA14</em>, which confers resistance to trimethoprim, was harbored in one copy in seven pESI or pESI-like plasmids, but the pM6–2-F5 plasmid harbored two copies. Additionally, the copy number of insertion sequence 26 in seven pESI-like plasmids was higher than in the pESI plasmid. In this study, 40 <em>S</em>. Infantis strains carrying the pESI or pESI-like plasmids from various countries, including two strains in Korea, were classified into two clusters, with the plasmids identified as IncI1 ST71 in cluster I and IncI1 ST209 in cluster II. The 16 strains representing IncI1 ST71 harbored <em>bla</em>_CTX-M-65, whereas the 8 strains representing IncI1 ST209 harbored <em>bla</em>_CTX-M-1. Our study provides valuable information on the pESI-like plasmid in <em>S</em>. Infantis from the poultry industry worldwide.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110576"},"PeriodicalIF":2.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic regulation of short chain fatty acid on Leghorn male hepatoma cells infected with Fowl Adenovirus serotype-4","authors":"Rangyeon Lee ,&nbsp;Hyunjin Song ,&nbsp;Junghoon Kim ,&nbsp;Hann Woo Sung ,&nbsp;Hyuk Moo Kwon ,&nbsp;Christopher A. Hunter ,&nbsp;Jeongho Park","doi":"10.1016/j.vetmic.2025.110571","DOIUrl":"10.1016/j.vetmic.2025.110571","url":null,"abstract":"<div><div>Fowl Adenovirus serotype 4 (FAdV-4) infection causes hepatitis-hydropericardium syndrome (HHS), a disease with high mortality in poultry. Short chain fatty acids (SCFAs) are a major gut metabolite that strengthens barrier function, impacts microbiome dynamics, and enhances protective immunity. Among SCFAs, butyrate has been suggested to promote anti-inflammatory effect that reduces tissue damage during viral infections. In this study, we pre-treated Leghorn male hepatoma (LMH) cell line with butyrate, infected them with FAdV-4, and examined genetic regulation related to protective immunity. Differentially expressed genes (DEGs) were identified through RNA sequencing, revealing significant alterations in various cellular pathways during FAdV-4 infection. Butyrate restored the expression of several genes involved in metabolic pathways, including the PPAR pathway. Additionally, butyrate reduced the expression of the anti-viral molecule <em>Oasl</em> and while upregulating i<em>l8</em>, which plays a role in immune cell recruitment. These findings suggest that butyrate supports a beneficial antiviral response in host cells during FAdV-4 infection. This study provides the role of SCFAs in modulating host defense mechanisms against avian viral infections.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110571"},"PeriodicalIF":2.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role and regulation of membrane efflux pumps in Staphylococcus pseudintermedius clinical isolates","authors":"Elisa Rampacci ,&nbsp;Tommaso Felicetti ,&nbsp;Francesco Birettoni ,&nbsp;Riccardo Zelli ,&nbsp;Rolando Arcelli ,&nbsp;Stefano Sabatini ,&nbsp;Fabrizio Passamonti","doi":"10.1016/j.vetmic.2025.110574","DOIUrl":"10.1016/j.vetmic.2025.110574","url":null,"abstract":"<div><div>The overexpression of efflux pumps (EPs) is one of the strategies through which <em>Staphylococcus</em> spp. survive antibiotic treatment. This process is regulated by intricate and still not fully understood mechanisms. This study aimed to evaluate the efflux capability of <em>Staphylococcus pseudintermedius</em> clinical isolates, investigate its implications in multidrug resistance, and elucidate the molecular mechanisms underlying EP expression. Additionally, potential therapeutic approaches for inhibiting staphylococcal EPs were assessed. Phenotypic characterization of efflux activity included ethidium bromide (EtBr) MIC screening, fluorometry-based accumulation and efflux assays, and synergy tests with EP inhibitors (EPIs). The genetic basis of high efflux phenotypes was explored using WGS and qPCR. Based on EtBr MIC, nine out of 109 isolates (8.3 %) progressed to fluorometry tests, confirming high phenotypic efflux in six isolates (Spearman r_s= 0.886). These were classified into distinct multilocus sequence types (ST71, ST496, ST551 and ST1062), with two isolates representing novel STs, designated ST2837 and ST2838. Synergism was observed when combining an EPI with EtBr, ciprofloxacin, clindamycin, and chloramphenicol; however, no EPI restored full susceptibility to these antibiotics. All isolates contained at least one plasmid-encoded EP gene, with <em>qacG</em> being the most common (6/6), followed by <em>qacH</em> (3/6), <em>tetK</em> (3/6), <em>smr</em> (2/6), <em>qacJ</em> (1/6), and <em>msrA/B</em> (1/6). Variable expression of EP genes was noted, with MFS-JC286_RS07290 overexpressed in all isolates, while MFS-JC286_RS11100 and MFS-JC286_RS11015 showed the highest expression levels. Comparative alignments suggested that the observed high efflux is unlikely due to mutational events upstream. These findings imply a potential role for transcriptional regulators, highlighting new research directions in this area.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"306 ","pages":"Article 110574"},"PeriodicalIF":2.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring of Bartonella spp. in Iberian bats from Spain","authors":"Clara Muñoz-Hernández ,&nbsp;Víctor Luque-Castro ,&nbsp;Marta Sánchez-Sánchez ,&nbsp;Jorge Sereno-Cadierno ,&nbsp;Xosé Pardavila ,&nbsp;Joaquín Vicente ,&nbsp;Isabel G. Fernández de Mera ,&nbsp;Alberto Moraga-Fernández","doi":"10.1016/j.vetmic.2025.110573","DOIUrl":"10.1016/j.vetmic.2025.110573","url":null,"abstract":"<div><div>Chiroptera play a key role in the environment by providing pivotal ecosystem services. However, bats can be hosts of several zoonotic pathogens, including bacteria belonging to the <em>Bartonella</em> genus. This molecular study aimed to fill the notable lack of information concerning <em>Bartonella</em> spp. in bats from the Iberian Peninsula. We investigated the occurrence, risk factors and molecular characterization of this pathogen in 240 live bats sampled in northern, central, and southern areas of Spain during 2020–2021. Blood DNA samples from 22 bat species of Miniopteridae, Vespertilionidae and Rhinolophidae were analyzed using PCR assays and Sanger sequencing. The results showed a <em>Bartonella</em> spp. prevalence of 17.1 % (41/240), with at least one positive individual in 13 bat species of six genera. <em>Miniopterus schreibersii</em> showed the highest prevalence (40.5 %; 95 % CI: 24.7–56.4), and to the authors’ knowledge, <em>Bartonella</em> spp. is reported for the first time in <em>Barbastella barbastellus</em>, <em>Nyctalus lasiopterus</em> and <em>Nyctalus leisleri</em>. Infestation with ectoparasites (ticks, bat flies and/or mites) and bat family (Miniopteridae) were significantly associated with <em>Bartonella</em> prevalence. Sequencing revealed genetic similarities with <em>Bartonella</em> sp. sequences reported from bats and bat ectoparasites of various regions (e.g. Finland, France, South Korea, China, Georgia, Czech Republic or United Kingdom). Moreover, potential new species, distant from all other published sequences, were identified. In conclusion, <em>Bartonella</em> spp. infection is widely distributed within bat populations across peninsular Spain, and further work should be conducted to better understand the <em>Bartonella</em> species diversity circulating in Iberian bats.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"307 ","pages":"Article 110573"},"PeriodicalIF":2.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An engineered canine-mouse chimeric neutralizing antibody provides therapeutic effects against canine parvovirus infection","authors":"Zhihao Wang ,&nbsp;Pengfei Shi ,&nbsp;Ying Li ,&nbsp;Sheng Wang ,&nbsp;Yuanbao Zhou ,&nbsp;Chengguang Zhang ,&nbsp;Ling Zhao ,&nbsp;Sizhu Suolang ,&nbsp;Jiahui Zou ,&nbsp;Hongbo Zhou","doi":"10.1016/j.vetmic.2025.110572","DOIUrl":"10.1016/j.vetmic.2025.110572","url":null,"abstract":"<div><div>Canine parvovirus (CPV) is a highly contagious and severe infectious disease that can lead to hemorrhagic enteritis and even acute death in dogs. Despite mouse monoclonal antibodies (mAbs) have been employed in clinical treatment, their application in non-murine species is restricted due to immune rejection. In this study, we screened a mouse mAb (5E7) with high neutralizing activity against CPV using hybridoma technology. Subsequently, the variable regions of the heavy (VH) and light (VL) chains of 5E7 were amplified by PCR and fused with the constant regions of canine IgG antibody to produce canine-mouse chimeric antibody (CM-5E7). The chimeric antibody was successfully expressed in HEK293 cells and exhibited high neutralizing activity against multiple CPV subtypes <em>in vitro</em>. Furthermore, CM-5E7 exhibited effective therapeutic potential in dogs subjected to lethal dose CPV-2c challenge <em>in vivo</em>. Overall, CM-5E7 demonstrated high neutralizing activity against CPV and showed significant efficacy in treating CPV-2c infections, positioning it as a promising candidate therapeutic antibody for the treatment of CPV infection.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"306 ","pages":"Article 110572"},"PeriodicalIF":2.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}