Veterinaria italiana最新文献_第8页

Detection and dynamics of tumor necrosis factor alpha in the diagnosis and treatment of canine heartworm disease. 在犬心丝虫病的诊断和治疗中检测肿瘤坏死因子 alpha 及其动态变化。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.2662.22847.2

Ljubica Spasojević Kosić, Vuk Vracar, Gordana Kozoderovic, Vesna Lalosevic

{"title":"Detection and dynamics of tumor necrosis factor alpha in the diagnosis and treatment of canine heartworm disease.","authors":"Ljubica Spasojević Kosić, Vuk Vracar, Gordana Kozoderovic, Vesna Lalosevic","doi":"10.12834/VetIt.2662.22847.2","DOIUrl":"10.12834/VetIt.2662.22847.2","url":null,"abstract":"The aim of this study was to determine the concentration of TNF-alpha (TNF-α) in dogs naturally infected with Dirofilaria immitis (D. immitis) and to assess whether there are any changes in TNF-α concentration and their dependence during therapy for heartworm disease (HWD). For this study, 14 client-owned dogs with HWD were selected. Clinical and parasitological examinations (modified Knott test for circulating microfilariae and SNAP Test IDEXX for circulating D. immitis antigen) had been used for diagnosing D. immitis and HWD. All dogs were treated with an alternative therapy for HWD (oral doxycycline 10 mg/kg b.w., once daily for 6 weeks, then alternately 4 weeks without and 2 weeks with the medication, and oral ivermectin 6-14 µg/kg b.w., every 2 weeks). The dogs blood sera at the moment of HWD diagnosis, during and at the end of therapy were frozen for further quantifying of TNF-α (Canine TNF-alpha ELISA kit, Thermo scientific). At the moment of HWD diagnosis TNF-α was detected in 9 dogs (7.21±12.44 pg/ml). Concentration of TNF-α was not significantly change during the therapy, neither related to the level of D. immitis antigen nor to antigen level changes. The alternative therapy for HWD has no influence on TNF-α concentration dynamics.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic evaluation of nested PCR and microscopy for Cryptosporidiosis in goats: can COWP gene based qRT-PCR be useful in assessment of active Cryptosporidial infections? 巢式 PCR 和显微镜对山羊隐孢子虫病的诊断评估：基于 COWP 基因的 qRT-PCR 是否有助于评估活动性隐孢子虫感染？

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3056.22638.1

Supriya Sachan, Gururaj Kumaresan, Dinesh Kumar Sharma, Giridhari Das, Suman Kumar, Ravi Khare, Anjali Pachoori, Souvik Paul

{"title":"Diagnostic evaluation of nested PCR and microscopy for Cryptosporidiosis in goats: can COWP gene based qRT-PCR be useful in assessment of active Cryptosporidial infections?","authors":"Supriya Sachan, Gururaj Kumaresan, Dinesh Kumar Sharma, Giridhari Das, Suman Kumar, Ravi Khare, Anjali Pachoori, Souvik Paul","doi":"10.12834/VetIt.3056.22638.1","DOIUrl":"10.12834/VetIt.3056.22638.1","url":null,"abstract":"The present research delved into the transmission patterns, diagnostic methods, molecular traits, and phylogenetic analysis of Cryptosporidium species. The research was undertaken to enhance comprehension of the epidemiology and the potential for zoonotic transmission. A total of 80 goat-kid samples were tested, 7 were confirmed positive by mZN microscopy and 12 by nested-PCR. By PCR, 18SSUrRNA, HSP70, and GP60 amplicons were tested for Cryptosporidium. The restriction enzymes viz., SspI, VspI and MboII were used to genotype 12 Cryptosporidium positive samples by which C. parvum and C. bovis mixed infections were detected. Quantitative reverse transcription real-time PCR was used to transcriptionally screen the COWP-subunit genes to assess the severity of the infection in goat-kids, which showed upregulation of COWP6 and COWP4, while COWP9 and COWP3 genes were downregulated. A silent mutation was found at the codon CCA→CCC, which is being reported for the first time in goat field isolates. Phylogenetic and sequencing analyses confirmed the presence of the anthropozoonotic IIe subtype.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ELISA in raw milk for detection of antibodies to Mycobacterium avium subsp. paratuberculosis in dairy herd. 评估用酶联免疫吸附法在生奶中检测奶牛副结核分枝杆菌抗体的效果。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.2948.18971.1

Silvina Tieri, Karina Cirone, Claudia Morsella, Laura Méndez, Silvia Mundo, Bárbara Fernández, Fernando Paolicchi

{"title":"Evaluation of ELISA in raw milk for detection of antibodies to Mycobacterium avium subsp. paratuberculosis in dairy herd.","authors":"Silvina Tieri, Karina Cirone, Claudia Morsella, Laura Méndez, Silvia Mundo, Bárbara Fernández, Fernando Paolicchi","doi":"10.12834/VetIt.2948.18971.1","DOIUrl":"https://doi.org/10.12834/VetIt.2948.18971.1","url":null,"abstract":"Paratuberculosis (PTBC) is a chronic intestinal disease of animals caused by Mycobacterium avium paratuberculosis (MAP). MAP infection is diagnosed through indirect tests based on the immune response. The aims of this study were to compare the performance of two milk ELISA for the diagnosis of PTBC and to assess the bulk tank milk (BTM) MAP exposure in dairy cattle in Argentina. A total of 357 fecal, serum, and milk samples were collected. The fecal samples were processed by culture for MAP isolation, while both, serum and milk samples were used for the detection of antibodies by two different ELISA tests, \"in-house\" and commercial kit. MAP was isolated in 3.9% of fecal samples. For milk ELISA, poor concordances were obtained. Optimized cut-off points were calculated. The highest sensitivity and specificity values (64% and 80% respectively) were obtained with the combination of MAP isolation and commercial milk ELISA. The results indicate that the combination of different techniques to identify of dairy cattle infected with MAP increases the efficiency of diagnosis. In addition, BTM samples (n=98) were evaluated to determine herd status using the commercial kit during two seasons, identifying 33.3% of positive samples in autumn and 35.4% in spring.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxigenic Staphylococcus aureus in some animal-originated food products marketed in Turkey: presence and Public Health concerns. 土耳其市场上销售的一些动物源性食品中的毒性金黄色葡萄球菌：存在情况和公共卫生问题。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3022.21271.2

Fulden Karadal, Nurhan Ertas Onmaz, Harun Hizlisoy, Yeliz Yildirim, Serhat Al, Zafer Gonulalan, Cemalettin Bagci

{"title":"Toxigenic Staphylococcus aureus in some animal-originated food products marketed in Turkey: presence and Public Health concerns.","authors":"Fulden Karadal, Nurhan Ertas Onmaz, Harun Hizlisoy, Yeliz Yildirim, Serhat Al, Zafer Gonulalan, Cemalettin Bagci","doi":"10.12834/VetIt.3022.21271.2","DOIUrl":"https://doi.org/10.12834/VetIt.3022.21271.2","url":null,"abstract":"This study aimed to detect the presence of Staphylococcus aureus in some animal source food (ASF) including emulsified meat products (sausage and salami), dry fermented meat product (soudjouk), semi dry meat product (pastrami) and raw chicken meat. Sixty six (38.8%) of 170 samples were found to be positive for S. aureus. It was determined that S. aureus was found in 17 (56.6%) salami, 27 (54%) raw chicken meat, 9 (30%) soudjouk, 9 (30%) pastrami, 4 (13.3%) sausage samples. Staphylococcal enterotoxins (SEs) were identified in 5 out of 66 (7.5 %) isolates food matrices including 3 (4.5%) SEA, 2 (3.03%) SEC. The sea and sec genes were detected in 3 (4.5%) of 66 isolates. The results of this study highlight the need to provide suitable control strategies concerning production, sales, and storage to prevent the spread of enterotoxigenic S. aureus isolates in ASF. The key contribution of this study is its revelation of the presence of S. aureus in animal products sold in Turkish local markets, highlighting the potential public health risks associated with animal foods.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of vesivirus in minks (Neovison vison), Italy 2021. 在水貂（Neovison vison）中检测到囊病毒，意大利 2021 年。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3472.23552.1

Paola Ripà, Francesco Perugini, Nicola Decaro, Valentina Curini, Addolorato Ruberto, Maurilia Marcacci, Vito Martella, Alessio Lorusso, Gianvito Lanave

引用次数: 0

Meat juice as a feasible alternative sample for tuberculosis surveillance in large game. 将肉汁作为监测大型野味中结核病的可行替代样本。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3132.22735.2

Ana Carolina Abrantes, David Risco, Miguel Carvalho, Rosario Cerrato, Pedro Fernández-Llario, Madalena Vieira-Pinto

引用次数: 0

Does a therapeutical dose of ivermectin impairs testicular homeostasis of rats via excessive apoptosis? 治疗剂量的伊维菌素会通过过度凋亡损害大鼠睾丸的稳态吗？

4区农林科学

Veterinaria italiana Pub Date : 2024-02-20 DOI: 10.12834/VetIt.2692.19722.2

Flora Cordeiro, Flaviane L Ceglio, Nathalia A Galvão, Leoni V Bonamin, Eduardo F Bondan, Thiago Berti Kirsten, Maria M Bernardi

{"title":"Does a therapeutical dose of ivermectin impairs testicular homeostasis of rats via excessive apoptosis?","authors":"Flora Cordeiro, Flaviane L Ceglio, Nathalia A Galvão, Leoni V Bonamin, Eduardo F Bondan, Thiago Berti Kirsten, Maria M Bernardi","doi":"10.12834/VetIt.2692.19722.2","DOIUrl":"10.12834/VetIt.2692.19722.2","url":null,"abstract":"Ivermectin is a medication used to treat parasite infestations in humans and in veterinary medicine. Previously we showed that therapeutical doses of ivermectin impaired spermatogenesis and spermiogenesis in adult rats. The present study was proposed to understand the pathophysiological mechanism that triggered these impairments induced by ivermectin. It was a particular objective to study if ivermectin induced excessive apoptosis. Adult rats were treated with a therapeutical dose of ivermectin (subcutaneously). Their testis was evaluated for the expression of caspase-3 (a marker of apoptosis), using immunohistochemistry techniques. Results revealed that ivermectin treatment increased the expression of caspase-3 (labeled seminiferous tubules and strongly labeled tubules), as well as increased the number of tubules that presented labeled cells in the tubular lumen, compared to the data of the control group. In conclusion, a therapeutical dose of ivermectin induced expressive apoptosis in cells of the seminiferous tubules of rats, affecting the testicular natural homeostasis process, which resulted in the spermatogenesis and spermiogenesis impairments previously reported.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic Resistance Profile of Rarely Isolated Salmonella Serotypes from Poultry in Turkey. 土耳其家禽中罕见沙门氏菌血清型的英语抗生素耐药性概况。

4区农林科学

Veterinaria italiana Pub Date : 2023-12-31 DOI: 10.12834/VetIt.3004.20698.2

Hamit Kaan Müştak, Seyyide Sarıçam İnce, İnci Başak Müştak

{"title":"Antibiotic Resistance Profile of Rarely Isolated Salmonella Serotypes from Poultry in Turkey.","authors":"Hamit Kaan Müştak, Seyyide Sarıçam İnce, İnci Başak Müştak","doi":"10.12834/VetIt.3004.20698.2","DOIUrl":"10.12834/VetIt.3004.20698.2","url":null,"abstract":"This study investigated five strains of each serotype of Salmonella Agona, Salmonella Heidelberg, Salmonella Hindmarsh, Salmonella Kouka, Salmonella Muenchen, Salmonella Ottmarchen, Salmonella Saintpaul and Salmonella II, isolated during the 2014-2017 period. Disc diffusion was used to identify the phenotypic profiles of antibiotic resistance to 12 antimicrobials while the presence of antibiotic resistance genes (ARGs) was detected by PCR. The most sensitive serotype was S. Kouka while the most resistant serotypes were S. Agona and S. Heidelberg. MDR was detected most frequently in S. Agona strains, followed by S. Saintpaul, S. Hindmarsch, and S. Ottmarchen. The samples were most susceptible to chloramphenicol and ceftazidime and most resistant to sulfonamide. The resistance genes were detected in phenotypically resistant strains. Among the tetracycline-resistant strains, tet (A) was the most prevalent gene. The results of this study highlight the importance of monitoring antibiotic resistance profiles and related genes, which can spread to form MDR bacteria. Salmonella spp., which significantly contribute to ARG dissemination, should be monitored constantly to protect the closely related health of humans, animals, and the environment. The level of antibiotic resistance observed in this study, even in rarely isolated Salmonella serotypes, also indicates the need for careful and selective use of antibiotics.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Reticuloendotheliosis Virus from Chorio-allantoic Membrane of SPF Chicken Eggs inoculated with Fowl Pox Virus. 从接种了鸡痘病毒的 SPF 鸡卵绒毛尿囊膜中分离网状内皮病病毒

4区农林科学

Veterinaria italiana Pub Date : 2023-12-31 DOI: 10.12834/VetIt.3164.21331.2

Nilabja Roy Chowdhury, Bimalendu Mondal, Sanchay K Biswas, Apratim Maity, Kunal Batabyal, Subhasis Batabyal

{"title":"Isolation of Reticuloendotheliosis Virus from Chorio-allantoic Membrane of SPF Chicken Eggs inoculated with Fowl Pox Virus.","authors":"Nilabja Roy Chowdhury, Bimalendu Mondal, Sanchay K Biswas, Apratim Maity, Kunal Batabyal, Subhasis Batabyal","doi":"10.12834/VetIt.3164.21331.2","DOIUrl":"https://doi.org/10.12834/VetIt.3164.21331.2","url":null,"abstract":"Fowl Pox Viruses (FPV) infect chickens and turkeys giving rise to pock lesions on various body parts like combs, wattles, legs, shanks, eyes, mouth etc. The birds, affected with FPV, also show anemia and ruffled appearance which are clinical symptoms of Reticuloendotheliosis. Interestingly, the field strains of FPV are integrated with the provirus of Reticuloendotheliosis Virus (REV). Due to this integration, the infected birds, upon replication of FPV, give rise to free REV virions, causing severe immunosuppression and anemia. Pox scabs, collected from the infected birds, not only show positive PCR results upon performing FPV-specific 4b core protein gene PCR but also show positive results for the PCR of REV-specific env gene and FPV-REV 5'LTR junction. Homogenized suspension of the pock lesions, upon inoculating to the Chorio-allantoic Membrane (CAM) of 10 days old specific pathogen-free embryonated chicken eggs, produces characteristic pock lesions in serial passages. But the lesions also harbor REV mRNA or free virion, which can be identified by performing REV-specific env gene PCR using REV RNA from FPV-infected CAMs. The study suggests successful replication and availability of REV mRNA and free virion alongside the FPV virus, although the CAM is an ill-suited medium for any retroviral (like REV) growth and replication.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of Salmonella enterica in Pakistani backyard poultry breeds: isolation, molecular characterization, and pathology. 巴基斯坦散养家禽品种中肠炎沙门氏菌的证据：分离、分子特征和病理学。

4区农林科学

Veterinaria italiana Pub Date : 2023-12-31 DOI: 10.12834/VetIt.2870.19149.3

Muhammad Bilal, Shahan Azeem, Asim Aslam, Shahid Abbas, Muhammad Ilyas Riaz, Faisal Shahzad, Muhammad Yasin Tipu

{"title":"Evidence of Salmonella enterica in Pakistani backyard poultry breeds: isolation, molecular characterization, and pathology.","authors":"Muhammad Bilal, Shahan Azeem, Asim Aslam, Shahid Abbas, Muhammad Ilyas Riaz, Faisal Shahzad, Muhammad Yasin Tipu","doi":"10.12834/VetIt.2870.19149.3","DOIUrl":"https://doi.org/10.12834/VetIt.2870.19149.3","url":null,"abstract":"The present study evaluated the presence of Salmonella enterica in Pakistani backyard poultry. A total 48 chickens from 4 backyard poultry breeds with the clinical presentation of S. enterica infection were randomly selected from villages in the Punjab Province. Cloacal swabs from live poultry and liver samples from the dead birds were collected for bacterial culture and biochemical identification. Liver and spleen samples from dead birds were evaluated for gross and histopathological changes. Bacterial isolates were subjected to PCR and sequencing of ratA gene. Biochemical identification revealed 5/48 (10.42%) chickens positive for S. enterica. Gross pathology included enlarged, discoloured and congested liver and congested spleen. Histopathology demonstrated congestion of sinusoidal capillaries, cellular swelling and cellular/ballooning degeneration, congestion of central hepatic vein, granular hepatocytic cytoplasm and the presence of variable-sized vacuoles in hepatocytes. The PCR yielded a S. enterica specific amplicon (1047 bp). All liver samples that were positive for S. enterica by biochemical tests, were also positive by PCR. The ratA gene sequencing revealed a close resemblance with S. enteritidis isolates from humans. The present study highlights zoonotic risk from backyard poultry and suggests that PCR can be used as an alternate method for rapid detection of Salmonella serovars.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0