Veterinaria italiana最新文献_第7页

Isolation and genetic characterization of parvoviruses from domestic cats reveals emergence of CPV-2c in India: A first report. 家猫副病毒的分离和遗传特征描述揭示了 CPV-2c 在印度的出现：首次报告。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-07-31 DOI: 10.12834/VetIt.3430.23463.2

Suvendu Kumar Behera, Mary H Lalhriatchhungi, Pradyumna Chakraborty, Parimal Roychoudhury, Leibaknganbi Maibam, Parthasarathi Behera, Jitendra Kumar Chaudhary, Hridayesh Prasad, J B Rajesh, Kalyan Sarma, Chethan G E, Nirali Piyush Shah, Wanta Khuman Maibam, Ravindra Kaka Jadhav, Lalhmangaihzuali

{"title":"Isolation and genetic characterization of parvoviruses from domestic cats reveals emergence of CPV-2c in India: A first report.","authors":"Suvendu Kumar Behera, Mary H Lalhriatchhungi, Pradyumna Chakraborty, Parimal Roychoudhury, Leibaknganbi Maibam, Parthasarathi Behera, Jitendra Kumar Chaudhary, Hridayesh Prasad, J B Rajesh, Kalyan Sarma, Chethan G E, Nirali Piyush Shah, Wanta Khuman Maibam, Ravindra Kaka Jadhav, Lalhmangaihzuali","doi":"10.12834/VetIt.3430.23463.2","DOIUrl":"https://doi.org/10.12834/VetIt.3430.23463.2","url":null,"abstract":"The objective of the present study was to isolate and characterize the VP2 gene of parvoviruses from domestic cats in India. For that, 38 fecal samples were screened by PCR with 36.84% positivity. Sequence analysis of those isolates showed canine parvovirus type-2c (CPV-2c) as the predominant variant, followed by feline panleukopenia virus (FPV) and 2a. Phylogenetic analysis of the CPV-2c sequences revealed clustering with Singaporean, South Korean, Mongolian and Bangladeshi dog 2c sequences. Phylogenetic analysis of the 2a isolate (MZC 2) was found to be clustered with Indian, Thai and Singaporean dog 2a isolates. Similarly, all the four FPV sequences were ancestrally related to Indian dog and cat FPV sequences hinting towards interspecies transmission between dogs and cats. Both synonymous and non-synonymous mutations were evident in CPV-2c, 2a and FPV sequences indicative of active evolution. In cell culture medium, CPV-2 showed cytopathogenic effects at the third passage level. In conclusion, the study provided the first report of CPV-2c in cats from India, which demands for extensive epidemiological surveillance to monitor interspecies spread and to shed more light on viral phylogenomics, their distribution in the country and in the Southeast Asian region and usage of current vaccines.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canine trypanosomosis cases: monitor lizard as an unusual vector. 犬锥虫病病例：巨蜥是不寻常的病媒。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-07-31 DOI: 10.12834/VetIt.3403.22962.2

Olutoye Adegboye, Olusegun Adegboye

{"title":"Canine trypanosomosis cases: monitor lizard as an unusual vector.","authors":"Olutoye Adegboye, Olusegun Adegboye","doi":"10.12834/VetIt.3403.22962.2","DOIUrl":"10.12834/VetIt.3403.22962.2","url":null,"abstract":"Trypanosomosis is a well-known sub-Saharan disease. The human form was discovered in The Gambia over 100 years ago. Canine trypanosomosis in The Gambia has never been mentioned in the scientific literature, let alone the involvement of veranus species in its transmission to dogs. The disease's most important vector is the tsetse fly. This fly is abundant in The Gambia, and its infamy for transmitting the disease has been well established. A lot of research efforts have been put into understanding the critical role of this pest in the transmission of the protozoan and the disease in livestock. This report confirms the presence of the disease in domestic dogs in The Gambia, and three canine cases with varied clinical signs, different hematological pictures accompanying the disease, and different effective treatment approaches are reported. Early detection can prevent severe illness and help patients to recover better. This report enhances our understanding on canine trypanosomosis, transmission of the pathogen, and strategies for managing the disease. This report is significant, as it is the first mention of monitor lizards in the 'transmission of trypanosome parasites to dogs during the fighting between them.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity of BatIFIT5 in different species of healthy and naturally infected with rabies virus bats. 不同健康和自然感染狂犬病病毒的蝙蝠中BatIFIT5的活性。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-07-31 DOI: 10.12834/VetIt.3427.23374.1

Camila Mosca Barboza, Juliana Santos Lima, Bianca Ferreira Gomes, Jaine Gonçalves Garcia, Tatiane de Cássia Pardo Souza, Raphaela Mello Zamudio, Maria Eduarda Rodrigues Chierato, Rafael De Novaes Oliveira, Pedro Carnieli Junior, Maria Cristina Carlan da Silva, Willian Oliveira Fahl, Enio Mori, Karin Correa Scheffer, Paulo Michel Roehe, Ana Claudia Franco, Helena Beatriz De Carvalho Ruthner Batista

{"title":"Activity of BatIFIT5 in different species of healthy and naturally infected with rabies virus bats.","authors":"Camila Mosca Barboza, Juliana Santos Lima, Bianca Ferreira Gomes, Jaine Gonçalves Garcia, Tatiane de Cássia Pardo Souza, Raphaela Mello Zamudio, Maria Eduarda Rodrigues Chierato, Rafael De Novaes Oliveira, Pedro Carnieli Junior, Maria Cristina Carlan da Silva, Willian Oliveira Fahl, Enio Mori, Karin Correa Scheffer, Paulo Michel Roehe, Ana Claudia Franco, Helena Beatriz De Carvalho Ruthner Batista","doi":"10.12834/VetIt.3427.23374.1","DOIUrl":"https://doi.org/10.12834/VetIt.3427.23374.1","url":null,"abstract":"Bats are mammals with vital role played in numerous ecosystem services, however bats can be important reservoirs or hosts for several microorganisms. Rabies is a zoonosis caused by Rabies lyssavirus (RABV) that affects the central nervous system (CNS) of all mammals, including bats and humans. The action of IFN-stimulated genes (ISGs) could be responsible for inhibiting different stages of the viral replication cycle. A major family of ISGs are the Interferon-induced proteins with tetrapeptide repeats (IFITs) and your action against infections caused by viruses from different families was proven. This study describes the expression of BatIFIT5 by RT-qPCR in different species of healthy and naturally infected with RABV bats. A total, of 36 bats were analyzed (18 positive and 18 negative for rabies) and 16 (44.44%) were positive for BatIFIT5. Here we analyzed fourteen species of bats with different eating and behavioral habits. Seven genetics lineages of RABV were evaluated and included in these 14 species of bats, no cases of RABV spillover were identified. In addition, we did not verified relationship between the bat species expression of BatIFIT5 and RABV. Many points about immunology of bats are unknown and here we analyzed one of these points.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic resistance, virulence genes, and phylogenetic groups of bacteria isolated from wild passerine birds in Iran. 从伊朗野生百灵鸟体内分离的细菌的抗生素耐药性、毒力基因和系统发生群。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-07-31 DOI: 10.12834/VetIt.2984.22731.2

Maliheh Mousavinezhad, Mansour Aliabadian, Mohammad Reza Sharifmoghadam, Masoumeh Bahreini, Jonas Waldenström

{"title":"Antibiotic resistance, virulence genes, and phylogenetic groups of bacteria isolated from wild passerine birds in Iran.","authors":"Maliheh Mousavinezhad, Mansour Aliabadian, Mohammad Reza Sharifmoghadam, Masoumeh Bahreini, Jonas Waldenström","doi":"10.12834/VetIt.2984.22731.2","DOIUrl":"https://doi.org/10.12834/VetIt.2984.22731.2","url":null,"abstract":"Wild passerine birds may serve as environmental reservoirs and as vectors for the long-distance dispersal of microorganisms and resistance determinants. However, there is no much knowledge on pathogenic bacteria in wild birds in Iran. The present study aimed to analyze antibiotic resistance in wild passerine birds collected from the northeast region of Iran as the rich breeding bird fauna with a special focus on Escherichia coli virulence, integron, and phylogenetic groups. A total of 326 isolates were collected and identified from the cloaca of wild birds using a swab. The results showed a high percentage of resistance to tetracycline (45.8%) and ampicillin (26.7%). The resistance genes, tet(A), tet(B), tet(M), and tet(L) were detected in tetracycline-resistant isolates, while the blaTEM gene was the most prevalent in ampicillin-resistant isolates (38.6%). Out of the 129 E. coli isolates examined, 99 isolates were found to have virulence gene, with the highest prevalence of the fimbriae (fimH) gene (22.4%). Additionally, the E. coli strains were most often classified into phylogenetic groups B1 (48.8%) followed by B2 (19.3%). Also, the highest average frequency of class 1 integron was detected among our isolates. Results indicated that wild birds are reservoirs of multidrug resistance and virulence genes that may have the potential to be transferred to other organisms, including humans.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategic design for Research and Innovation through a participatory approach: lessons learnt in a Veterinary Public Health Institution. 通过参与式方法进行研究与创新战略设计：从兽医公共卫生机构中汲取的经验教训。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-07-30 DOI: 10.12834/VetIt.2565.23329.2

Valeria Mariano, Romano Zilli, Eda Maria Flores Rodas, Raniero Lorenzetti, Annalisa Paternò, Marina Bagni, Evgeniya Titarenco

{"title":"Strategic design for Research and Innovation through a participatory approach: lessons learnt in a Veterinary Public Health Institution.","authors":"Valeria Mariano, Romano Zilli, Eda Maria Flores Rodas, Raniero Lorenzetti, Annalisa Paternò, Marina Bagni, Evgeniya Titarenco","doi":"10.12834/VetIt.2565.23329.2","DOIUrl":"https://doi.org/10.12834/VetIt.2565.23329.2","url":null,"abstract":"The article describes the results and the experience gained in using a participatory approach to design a strategic development plan (SDP) in a veterinary public health institute. The bottom-up approach utilised was co-designed between the units of \"Research and Innovation\" and \"Training\". It included a survey administered to the institute staff (570 people) to collect inputs on three relevant areas: i) innovative research topics; ii) innovation needed to improve services; iii) new tools to carry out activities. After the survey, the following criteria were used to prioritise the research topics: appropriateness, relevance, capacity, impact and innovativeness. Based on the priority topics identified, small working groups were set up on a voluntary basis. The working groups, following the EuropeAid method, co-designed a SDP, set over a three-year period, with indications on aims, objectives, outputs, activities, SMART indicators, means of verification and targets. The method proved useful in continuing the process of institutional innovation, especially by stimulating the participation and commitment of younger innovative staff at the bottom of the hierarchical pyramid. The integration of the described method into regular management procedures would be desirable, to achieve more effective results.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drivers and evolution of acaricide resistance and multi-resistance in two Ecuador's subtropical livestock farming areas. 厄瓜多尔两个亚热带畜牧业地区杀螨剂抗药性和多重抗药性的驱动因素和演变。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-07-24 DOI: 10.12834/VetIt.3471.23969.2

Ximena Pérez-Otáñez, Valeria Paucar-Quishpe, Claude Saegerman, Jorge Grijalva-Olmedo, Cecilia Pérez-Escalante, Linette Jácome, Carlos Rivera, Darío Cepeda-Bastidas, Susana Arciniegas-Ortega, Sandra Enríquez, Lenin Ron-Garrido, Richar Rodríguez-Hidalgo, Sophie O Vanwambeke

{"title":"Drivers and evolution of acaricide resistance and multi-resistance in two Ecuador's subtropical livestock farming areas.","authors":"Ximena Pérez-Otáñez, Valeria Paucar-Quishpe, Claude Saegerman, Jorge Grijalva-Olmedo, Cecilia Pérez-Escalante, Linette Jácome, Carlos Rivera, Darío Cepeda-Bastidas, Susana Arciniegas-Ortega, Sandra Enríquez, Lenin Ron-Garrido, Richar Rodríguez-Hidalgo, Sophie O Vanwambeke","doi":"10.12834/VetIt.3471.23969.2","DOIUrl":"https://doi.org/10.12834/VetIt.3471.23969.2","url":null,"abstract":"The management of cattle ticks, particularly Rhipicephalus microplus, poses a global challenge in subtropical regions like Ecuador due to its impact on meat and milk productivity, leading to economic losses. Misuse of acaricides has resulted in resistance and multi-resistance, diminishing their effectiveness. This study evaluated resistance to amitraz, alpha-cypermethrin, and ivermectin using the Larval Packet test, laboratory-reared tick larvae collected from cattle were tested. Data on farm management and tick control practices were gathered via a questionnaire in Northwest Pichincha and Quijos River Valley over two years. Resistance rates in the first year (2020-2021) were 67.21% for amitraz, 57.38% for ivermectin, and 67.21% for alpha-cypermethrin. One year later (2021-2022), resistance levels were 59.57% for amitraz, 57.45% for ivermectin, and 68.09% for alpha-cypermethrin, with multi-resistance rates at 67.21% and 65.96% respectively. No significant differences were found between years or locations. Analysis of larval survival data determined lethal doses for tested acaricides. The study emphasizes the association between the lack of acaricide rotation, the incorrect dosage, and the absence of non-chemical measures in tick management could be associated with the development of resistances in ticks. Likewise, this study promotes the need for collaborative efforts to improve control practices and maintain acaricide efficacy.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 4","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computing metrics to inform selection of candidate areas for a regionalized approach to bovine tuberculosis eradication in Ireland. 计算指标，为选择候选地区提供信息，以便在爱尔兰采用区域化方法根除牛结核病。

4区农林科学

Veterinaria italiana Pub Date : 2024-06-19 DOI: 10.12834/VetIt.3486.23927.2

Jamie Tratalos, Jamie Madden, Guy McGrath, Simon More

{"title":"Computing metrics to inform selection of candidate areas for a regionalized approach to bovine tuberculosis eradication in Ireland.","authors":"Jamie Tratalos, Jamie Madden, Guy McGrath, Simon More","doi":"10.12834/VetIt.3486.23927.2","DOIUrl":"https://doi.org/10.12834/VetIt.3486.23927.2","url":null,"abstract":"We describe the computation of metrics to inform the selection of areas for a regionalised approach to bovine tuberculosis eradication in Ireland. Our aim was not to recommend suitable regions but to elucidate the criteria used in metric selection and comment on the diversity of metric values amongst regions. The 26 counties of Ireland were compared using 20 metrics, grouped into five categories: region size and cattle population, herd fragmentation, cattle movement, bovine TB testing, badger population and control. Fragmentation metrics, measuring the proportion of herds with land in at least two counties, varied considerably by county, from 1% to 24 %. Between 25 % and 92 % of moves into herds came from a different county, illustrating the likely disruption in trade that a regionalized approach could entail. Cattle movement networks were combined with a risk model to calculate the proportion of moves which would be deemed risky under a risk-based trading regime and these results were compared to a more traditional approach based on the herd type and test history of each herd, with many fewer moves potentially restricted using the latter approach. We show how correlation between region size and some of the metrics complicates their interpretation.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the efficacy of a new inactivated vaccine against Staphylococcus aureus, Echerchia coli and Mycoplasma bovis mastitis in cows. 评估新型金黄色葡萄球菌、大肠埃希氏菌和牛支原体乳腺炎灭活疫苗的功效。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3083.21855.3

Hilal Zengin, Zeliha Keskin Alkaç, Yaser Vezir, Sadettin Tanyıldızı, Fatih Ahmet Korkak, Betül Dağoğlu Hark, Gurdal Dagoglu

{"title":"Evaluation of the efficacy of a new inactivated vaccine against Staphylococcus aureus, Echerchia coli and Mycoplasma bovis mastitis in cows.","authors":"Hilal Zengin, Zeliha Keskin Alkaç, Yaser Vezir, Sadettin Tanyıldızı, Fatih Ahmet Korkak, Betül Dağoğlu Hark, Gurdal Dagoglu","doi":"10.12834/VetIt.3083.21855.3","DOIUrl":"10.12834/VetIt.3083.21855.3","url":null,"abstract":"Staphylococcus aureus, Escherichia coli and Mycoplasma bovis are the most commonly isolated mastitis pathogens. The aim of this study was to evaluate the efficacy of a new mixed vaccine against mastitis caused by Staphylococcus aureus, Escherichia coli, and Mycoplasma bovis. For this purpose, a mixed inactivated vaccine was administered subcutaneously to 24 heifers as one dose (2 mL) on the 45th day before birth and the second dose 21 days later. In 9 heifers, 2 mL of PBS was administered as placebo instead of vaccine. Then, heifers were divided into 3 groups as 7 vaccinated and 3 unvaccinated animals. Staphylococcus aureus, Escherichia coli, and Mycoplasma bovis were administered to the groups through intramammary route. Three vaccinated heifers were considered the common control without bacteria in all groups. The parameters considered to assess the effect of vaccination were clinical findings, bacterial count in milk, somatic cell count, and antibody titers. Clinical signs were observed only in the unvaccinated placebo group. Bacteria count and somatic cell count in milk increased in vaccinated and unvaccinated heifers. However, this increase was less in vaccinated animals and gradually returned to the normal level. In the unvaccinated heifers, it was ever high. Serum antibody titers were measured before and after vaccination. Antibody titers were high in vaccinated heifers after vaccination and were negative in unvaccinated heifers. In conclusion, the mixed vaccine had beneficial effect against Staphylococcus aureus, Escherichia coli, and Mycoplasma bovis mastitis and stimulated the immune response of vaccinated heifers.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Peste des petits ruminants virus circulation in Uttarakhand, India: a step towards global eradication of PPR by 2030. 印度北阿坎德邦小反刍兽疫病毒传播调查：向 2030 年全球根除小反刍兽疫迈出的一步。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.2988.21721.3

Amisha Netam, Anuj Tewari, Rajesh Kumar

{"title":"Investigation of Peste des petits ruminants virus circulation in Uttarakhand, India: a step towards global eradication of PPR by 2030.","authors":"Amisha Netam, Anuj Tewari, Rajesh Kumar","doi":"10.12834/VetIt.2988.21721.3","DOIUrl":"https://doi.org/10.12834/VetIt.2988.21721.3","url":null,"abstract":"This study was conducted to estimate the seroprevalence of Peste des petits ruminants virus (PPRV) and to determine the virus distribution in unvaccinated goats in the Pantnagar region of Uttarakhand state, India. A total of 212 serum samples from goats were collected randomly from various villages in three districts (Udhamsingh Nagar, Nainital, and Almora) of Uttarakhand. Serum samples were tested for anti-PPRV antibodies by a commercially available kit. RNA was extracted from the clinical samples and it was subjected to one-step RT-PCR, followed by virus isolation from positive samples. A total of 41 animals from various villages were found to be seropositive with a prevalence rate of 19.33%. PPR outbreaks were also reported from the Tarai region of Uttarakhand, and detection by PCR confirmed PPRV in 8 goats. Two representative swab samples were subjected to virus isolation in Vero cells and both samples showed typical cytopathic effects. The present study shows that PPRV is circulating in the Tarai region of Uttarakhand and mass vaccination for PPR must be followed in this region to increase herd immunity to a protective level. To the best of our knowledge, this is the first investigation of PPRV seroprevalence in unvaccinated goats of Uttarakhand, India.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive genetic analysis of the first near-complete genome of bovine coronavirus and partial genome of bovine rotavirus in Türkiye through metagenomics. 通过元基因组学对土耳其首个近乎完整的牛冠状病毒基因组和牛轮状病毒部分基因组进行综合遗传分析。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3372.22817.2

Emel Aksoy, Ahmet Kursat Azkur, İbrahim Halil Miraloglu

{"title":"Comprehensive genetic analysis of the first near-complete genome of bovine coronavirus and partial genome of bovine rotavirus in Türkiye through metagenomics.","authors":"Emel Aksoy, Ahmet Kursat Azkur, İbrahim Halil Miraloglu","doi":"10.12834/VetIt.3372.22817.2","DOIUrl":"https://doi.org/10.12834/VetIt.3372.22817.2","url":null,"abstract":"Obtaining the complete or near-complete genome sequence of pathogens is becoming increasingly crucial for epidemiology, virology, clinical science and practice. This study aimed to detect viruses and conduct genetic characterization of genomes using metagenomics in order to identify the viral agents responsible for a calf's diarrhoea. The findings showed that bovine coronavirus (BCoV) and bovine rotavirus (BRV) are the primary viral agents responsible for the calf's diarrhoea. The current study successfully obtained the first-ever near-complete genome sequence of a bovine coronavirus (BCoV) from Türkiye. The G+C content was 36.31% and the genetic analysis revealed that the Turkish BCoV strain is closely related to respiratory BCoV strains from France and Ireland, with high nucleotide sequence and amino acid identity and similarity. In the present study, analysis of the S protein of the Turkish BCoV strain revealed the presence of 13 amino acid insertions, one of which was found to be shared with the French respiratory BCoV. The study also identified a BRV strain through metagenomic analysis and detected multiple mutations within the structural and non-structural proteins of the BRV strain, suggesting that the BRV Kirikkale strain may serve as an ancestor for reassortants with interspecies transmission, especially involving rotaviruses that infect rabbits and giraffes.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0