Isolation of Reticuloendotheliosis Virus from Chorio-allantoic Membrane of SPF Chicken Eggs inoculated with Fowl Pox Virus.

IF 0.5 4区 农林科学 Q4 VETERINARY SCIENCES
Nilabja Roy Chowdhury, Bimalendu Mondal, Sanchay K Biswas, Apratim Maity, Kunal Batabyal, Subhasis Batabyal
{"title":"Isolation of Reticuloendotheliosis Virus from Chorio-allantoic Membrane of SPF Chicken Eggs inoculated with Fowl Pox Virus.","authors":"Nilabja Roy Chowdhury, Bimalendu Mondal, Sanchay K Biswas, Apratim Maity, Kunal Batabyal, Subhasis Batabyal","doi":"10.12834/VetIt.3164.21331.2","DOIUrl":null,"url":null,"abstract":"<p><p>Fowl Pox Viruses (FPV) infect chickens and turkeys giving rise to pock lesions on various body parts like combs, wattles, legs, shanks, eyes, mouth etc. The birds, affected with FPV, also show anemia and ruffled appearance which are clinical symptoms of Reticuloendotheliosis. Interestingly, the field strains of FPV are integrated with the provirus of Reticuloendotheliosis Virus (REV). Due to this integration, the infected birds, upon replication of FPV, give rise to free REV virions, causing severe immunosuppression and anemia. Pox scabs, collected from the infected birds, not only show positive PCR results upon performing FPV-specific 4b core protein gene PCR but also show positive results for the PCR of REV-specific env gene and FPV-REV 5'LTR junction. Homogenized suspension of the pock lesions, upon inoculating to the Chorio-allantoic Membrane (CAM) of 10 days old specific pathogen-free embryonated chicken eggs, produces characteristic pock lesions in serial passages. But the lesions also harbor REV mRNA or free virion, which can be identified by performing REV-specific env gene PCR using REV RNA from FPV-infected CAMs. The study suggests successful replication and availability of REV mRNA and free virion alongside the FPV virus, although the CAM is an ill-suited medium for any retroviral (like REV) growth and replication.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria italiana","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.12834/VetIt.3164.21331.2","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Fowl Pox Viruses (FPV) infect chickens and turkeys giving rise to pock lesions on various body parts like combs, wattles, legs, shanks, eyes, mouth etc. The birds, affected with FPV, also show anemia and ruffled appearance which are clinical symptoms of Reticuloendotheliosis. Interestingly, the field strains of FPV are integrated with the provirus of Reticuloendotheliosis Virus (REV). Due to this integration, the infected birds, upon replication of FPV, give rise to free REV virions, causing severe immunosuppression and anemia. Pox scabs, collected from the infected birds, not only show positive PCR results upon performing FPV-specific 4b core protein gene PCR but also show positive results for the PCR of REV-specific env gene and FPV-REV 5'LTR junction. Homogenized suspension of the pock lesions, upon inoculating to the Chorio-allantoic Membrane (CAM) of 10 days old specific pathogen-free embryonated chicken eggs, produces characteristic pock lesions in serial passages. But the lesions also harbor REV mRNA or free virion, which can be identified by performing REV-specific env gene PCR using REV RNA from FPV-infected CAMs. The study suggests successful replication and availability of REV mRNA and free virion alongside the FPV virus, although the CAM is an ill-suited medium for any retroviral (like REV) growth and replication.

从接种了鸡痘病毒的 SPF 鸡卵绒毛尿囊膜中分离网状内皮病病毒
鸡痘病毒(FPV)会感染鸡和火鸡,使其身体各部位出现痘疹,如鸡冠、鸡腿、鸡腿、鸡腿、眼睛、嘴巴等。感染 FPV 的禽类还会出现贫血和皱褶,这些都是网状内皮病的临床症状。有趣的是,FPV 的野外毒株与网状内皮细胞增多症病毒(REV)的前病毒整合在一起。由于这种整合,受感染的鸟类在复制 FPV 时会产生游离的 REV 病毒,导致严重的免疫抑制和贫血。从感染禽类身上采集的痘痂不仅在进行 FPV 特异性 4b 核心蛋白基因 PCR 时呈阳性结果,而且在进行 REV 特异性 env 基因和 FPV-REV 5'LTR 交界处 PCR 时也呈阳性结果。将痘痕病变的匀浆悬液接种到 10 天龄的特异性无病原体胚胎鸡的绒毛尿囊膜(CAM)上,在连续传代中会产生特征性的痘痕病变。但病变部位也会携带 REV mRNA 或游离病毒,这可通过使用来自受 FPV 感染的 CAM 的 REV RNA 进行 REV 特异性 env 基因 PCR 鉴定出来。这项研究表明,尽管 CAM 不适合任何逆转录病毒(如 REV)的生长和复制,但 REV mRNA 和游离病毒可与 FPV 病毒一起成功复制和存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Veterinaria italiana
Veterinaria italiana VETERINARY SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信