Journal of Genetic Engineering and Biotechnology Research最新文献

{"title":"Appearance of Aedes Aegypti Mosquito in Alobeid Town, North Kordofan State,\u0000September–November 2018, Sudan","authors":"","doi":"10.33140/jgebr.02.01.02","DOIUrl":"https://doi.org/10.33140/jgebr.02.01.02","url":null,"abstract":"The most breeding of Aedes aegypti mosquitoes were at indoor and outdoor water environments, including waterholding containers such as vases, jars, bathtubs, tires, garden debris, leaves, and flowerpots. The aim of the study\u0000was to survey Aedes aegypti larval breeding habitats and analyze vector indices. The survey was conducted in Alobied\u0000town, North Kordofan State in the in the selected 17 sites during September–November 2018. The mosquito immature\u0000stage including larvae and pupal stage of the genus Aedes were collected from the surveyed positive containers by\u0000sweep nets. The finding of the study showed that Aedes aegypti was the only species detected in water containers.\u0000Out of 226 houses were inspected a number of 84 houses were found positive. From a total of 784 containers were\u0000inspected with 136 containers positive. The House index (HI) during the period from September-November, 2018\u0000was found to be 37.2%, the Breatu index (BI) was 10.7%, and container index (CI) was 17.3% while the pupal per\u0000demographic (P/D) was 0.05. No significance differences between Aedes aegypti indices during different months. It\u0000can be concluded that Aedes aegypti was prevalent in Alobied town. Urgent need is required for developing community\u0000ability to monitor their houses. In addition to health education sessions to provide correct health knowledge and\u0000consistent advice about proper behaviors for the community to fight Aedes aegypti vector","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"323 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131702047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indoor and Outdoor House Inspection Coupled with Community Mobilization\u0000Impact on Decreased Aedes Aegypti Entomological Indices during ChikngunyaDengue Outbreak Containment in Affected Localities of Kassala and Red Sea\u0000States, 2018-2019","authors":"","doi":"10.33140/jgebr.02.01.01","DOIUrl":"https://doi.org/10.33140/jgebr.02.01.01","url":null,"abstract":"Aedes aegypti is an important vector for dengue virus infection. Apart from dengue virus, Aedes aegypti is also the\u0000vector for transmission of other viruses presenting serious public health threats: chikungunya, and yellow fever. The\u0000present paper aimed to assess the impact of indoor and outdoor house inspection coupled with community mobilization\u0000on decreased Aedes aegypti entomological indices during Chikungunya-Dengue Outbreak containment in affected\u0000Localities of Kassala and Red Sea States, 2018-2019. The survey was conducted in Kassala and Red Sea State during\u0000outbreak of chikungunya and dengue. The survey was performed in the affected localities by dividing the localities into\u0000seven-day blocks from Saturday to Friday. All dwellings of each block were thoroughly checked by health promoters\u0000on the specific day. The sufficient numbers of promoters were available to cover all the houses in the affected areas.\u0000The study revealed that the reduction in house index from baseline in Kassala state was reduced by 90.4% in week 7.\u0000While the House index was reduced from baseline in Red Sea Sate by 87.1% in week 14. For container index it was\u0000reduced by 89.6% in Kassala State in week 7 compared to 89.0% in Red Sea State in week 14. The study concluded\u0000that indoor and outdoor inspection coupled with community mobilization has impact on reduced of Aedes aegypti\u0000indices mainly house index and container index.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129855146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of Interaction between the SR Proteins 9G8 and Repressor Splicing\u0000Factor 1 in the Alternative Splicing of Carnitine Palmitoyltransferase I in lipid\u0000Metabolism","authors":"","doi":"10.33140/jgebr.01.02.03","DOIUrl":"https://doi.org/10.33140/jgebr.01.02.03","url":null,"abstract":"The method of gene regulation underlying lipid metabolism related to obesity is poorly understood, yet alternative\u0000splicing (AS) appears to be an important mechanism for proper lipid storage. CPT1 (carnitine palmitoyltransferase I)\u0000is a beta-oxidation enzyme required for the breakdown of fatty acids. The gene coding for CPT1 is alternatively spliced\u0000to produce two different products that vary in their activity. When the splicing SR protein 9G8 is knocked down in the\u0000fat body of Drosophila, the CPT1 splicing pattern is altered. A linear search algorithm was developed that parsed\u0000through FASTA files of the CPT1 gene region and sought out sequences that matched known binding sequences of\u00009G8. We expected a result in exon 6A that would signal its inclusion but unexpectedly found a match in exon 5 of the\u0000CPT1 transcript. We theorized that 9G8 and the SR protein competitor RSF1, interact with one another for binding\u0000sites on the CPT1 gene to result in different isoforms. Additionally, TG data indicated that 9G8 and RSF1 KD flies\u0000displayed statistically significant higher TG levels than their control suggesting a potential cooperative relationship.\u0000We next performed a starvation resistance experiment and found that 9G8 and RSF1 KD flies phenocopied starvation\u0000resistance suggesting that both proteins interact to result in the same CPT1 product. We are using qPCR to determine\u0000if there is a difference in the AS of CPT1 among flies with decreased expression of 9G8, the SR protein antagonist\u0000RSF1 and the SR protein shuttle TRN-SR.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127152692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive Association of Mutations in VKORC1 and CYP2C9 Genes with Venous Thrombo-Embolism (VTE) in Indian Population: A Case Control Study","authors":"","doi":"10.33140/jgebr.01.02.01","DOIUrl":"https://doi.org/10.33140/jgebr.01.02.01","url":null,"abstract":"Background: Venous thrombo-embolism (VTE) refers to a blood clot that starts in vein. It is the third most common vascular disease in the world, after myocardial infarction (MI) and stroke, affecting millions of individuals every year. Two main clinical manifestations of VTE include deep vein thrombosis (DVT) and pulmonary embolism (PE), the later one being potentially fatal. Objective: The aim of this study is to investigate whether mutations in two genes VKORC1 and CYP2C9 and subsequent changes in their plasma levels can be used to predict the risk for venous thromboembolism (VTE). Method: A total of five polymorphisms in two genes VKORC1 and CYP2C9 were genotyped in approximately 145 VTE patients and 229 control subjects. The genotyping was done through PCR-RFLP method. Plasma concentrations of VKORC1 and CYP2C9 were estimated using ELISA technique. Results: Genotypic analysis of common polymorphisms in VKORC1 gene showed significantly higher percentage of GG genotype of -1639G>A, CC genotype of 1173 C>T and TT genotype of -497T>G polymorphism in control subjects compared to VTE patients. Also, significantly higher percentage of CC genotype in 416C>T and AA genotype in 1061 A>C polymorphism of CYP2C9*2 and CYP2C9*3 genes respectively was observed in VTE patients in comparison to healthy controls. In addition to genotypic significance, plasma concentrations of both VKORC1 and CYP2C9 were significantly higher in patients group. Conclusion: All the three polymorphisms in VKORC1 gene; -1639G>A, 1173 C>T and -497T>G and two polymorphism 416C>T and 1061A>C of CYP2C9 gene showed significant association with VTE pathophysiology","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132694925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Mystery of Canavan Disease: A 100 Year Chronicle of Key Events","authors":"","doi":"10.33140/jgebr.01.02.02","DOIUrl":"https://doi.org/10.33140/jgebr.01.02.02","url":null,"abstract":"In the early 1900’s, in a small geographical region in northeastern Europe, a mysterious early-onset human malady was\u0000brought to the attention of physicians. In this disease, children appeared normal at birth and met usual developmental\u0000milestones up to the age of about 4-5 months. At that time, infants began to lose muscle tone and could no longer\u0000control their head movements. The disease was progressive, and by age 2, characterized by enlargement of the head,\u0000spastic responses to stimulation, and a general listlessness. By age 3, blindness was apparent and there was extension\u0000and posturing of limbs due to uncontrolled muscle contractions. Death usually occurred between ages 3-4. In large\u0000family’s common at that time, there could be 2-3 afflicted children out of a total of 8-9 siblings. There is no metric\u0000to measure pain and suffering of CD children, but we can measure progress made over the past century in solving\u0000the mystery. At the present time, there are great expectations that a human cure is possible since a mouse model of\u0000CD was recently shown to be completely cured by an unusual genetic engineering outcome. In this short review are\u0000chronicled key findings made over the past 100 + years that have led to identification of the genetic and cellular\u0000etiology of CD, and the reasons for such encouragement.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115682355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnology, Visual Engineering, and the Visual Mind Mapping Algorithm","authors":"","doi":"10.33140/jgebr.01.01.05","DOIUrl":"https://doi.org/10.33140/jgebr.01.01.05","url":null,"abstract":"Visual Engineering and Visual Intelligence: Newtonian Gravity Law\u0000Visual Engineering and Visual Intelligence comes together with a\u0000similar mind mapping and a creative algorithm. The insight of a\u0000creative genius is that is a mean of understanding the physics and\u0000the proven experimental form of creativity. Newtonian is an ordinary\u0000space of time and space. It is the discovery and the geometry of color\u0000and outstanding implications of the visual arts and the sciences.\u0000It requires a focus and a visual geometry mathematical space. A\u0000three-dimensional opportunity connecting to the mathematic results\u0000of infinity physics. An intellectual capacity to invent generating\u0000an electromagnetic Maxwell’s prediction theory. For example,\u0000the position of earth space and time. The atomic, platonic turn of\u0000gravity and how the quantum electrodynamics mechanics affects\u0000the principia of energy and the universe [1].","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130643094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell and Markers","authors":"","doi":"10.33140/jgebr.01.01.04","DOIUrl":"https://doi.org/10.33140/jgebr.01.01.04","url":null,"abstract":"Stem cells have the ability to go through various cell divisions and also maintain undifferentiated state. Stem cells\u0000are Embryonic (Pluripotent) and adult stem cells. Pluripotent stem cells give rise to all tissues such as ectoderm,\u0000mesoderm and endoderm. Embryonic stem cells isolated from inner cell mass of embryo blastocyst. Adult stem cells\u0000are also undifferentiated cells present in adult organisms and repair the tissue when damaged occurs but number in\u0000less. Adult stem cells are present in bone marrow, adipose tissue, blood and juvenile state umbilical cord and tissue\u0000of specific origin like liver, heart, intestine and neural tissue. Embryonic stem cells from blastocyst have the ethical\u0000problems and tumorogenecity. These can be identified by flow cytometry. There are wide range of stem cell markers\u0000which are useful in identifying them. Most of the pluripotent cell markers are common with tumor cell markers which\u0000throws a challenge for certainty.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132462449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Trends in the Treatment of Grade II Furcation Defects by Using Second\u0000Generation Platelet Concentrates","authors":"Juan Pablo Pava Lozano, Manuel Alejandro Sabogal, Dayana Andrea Mora, Yuly Constanza Ortiz, Juan Pablo, Hinojosa, David Gutierrez, Ana Luisa Munoz","doi":"10.33140/jgebr.01.01.01","DOIUrl":"https://doi.org/10.33140/jgebr.01.01.01","url":null,"abstract":"The furcation defect is defined as the pathological reabsorption of interadicular bone that occurs in multi-rooted\u0000and bi-rooted teeth in advanced stages of periodontal disease, it represents a great challenge for dentists and\u0000specialists when treating them, due to their different anatomical variations such as: root trunk, furcation opening,\u0000root relationship, interdental and interadicular morphologic features, the interadicular separation and the angle of\u0000root distance, that can interfere in the response to treatment.\u0000Some surgical strategies to cover furcation defects and exposed roots include free gingival grafts, pedicle flaps,\u0000sub epithelial connective tissue grafts, and application of different biomaterial-based grafts. We present a Case\u0000report of a 49 year-old patient who was diagnosed with grade II furcation defects on teeth 46 and 47, which were\u0000treated with Platelet- Rich Fibrin (PRF) is an autograft obtained from a blood sample of the patient undergoing\u0000processing in a centrifuge machine plus a coronal displacement flap surgical technique.\u0000A mucoperiosteal partial superficial thickness flap was lifted up from affected teeth and PRF was obtained\u0000from a patient blood sample (10mL) in a glass tube without anticoagulant, which was immediately processed\u0000on centrifuge machine. The flap was repositioned to coronal level beyond the cement enamel line with 2 PRF\u0000membranes placed on the root surfaces and sutured. Morphometric, tomography and clinical measurement was\u0000performed 6 months after the procedure to analyze the interadicular molar zones.\u0000The surgery showed presence of hard and soft tissues evaluated clinically and tomographically with a significant\u0000coverage of p <0.05 in the fornix zones of the molars. On the interadicular area morphometric values shows that\u0000tooth 46 there was a decrease (0.0005) of -1.127 (2.104 ± 0.06 vs 0.977 ± 0.07) of defect and tooth 47 (0.0047)\u0000of -0.850 (1.891 ± 0.04) vs 1.041 ± 0.05) CONCLUSION Use of CDF together with PRF can be considered as\u0000a treatment option because it achieves a ostensibly osteoconductive, biocompatible function and reduces patient\u0000recovery time improving the prognosis of established defects.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127611028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure, Enzymatic Mechanism of Action, Applications, Advantages, Disadvantages\u0000and Modifications of Luciferase Enzyme","authors":"S. Bellah, Divine MensahSedzro, Hameed Akbar, S. M. S. Billah","doi":"10.33140/jgebr.01.01.03","DOIUrl":"https://doi.org/10.33140/jgebr.01.01.03","url":null,"abstract":"North American Firefly, Photinu spyralis, entertainment countless observers, probably because of human arrival mainland.\u0000Between the period of 1950s and 1980s, many young biologists spent their money as a firefly collector, first hired by a\u0000Professor William D McElroy at Johns Hopkins University, later became a member of the Sigma Firefly Club. In 1985,\u0000when Marlene DeLuca and her colleagues cloned cDNA encoding luciferase, a replacement source of the enzyme becomes\u0000available and later in labs around the world, many other organisms began to emit unique yellow-green glow as a result of\u0000firefly expression Luciferase is in their cells. Now, luciferase and its genes have become very useful for research purposes\u0000and also for a variety of commercial purposes. The structure of firefly and bacterial luciferase will be of great value for\u0000the development of applications in many processes. There are many firefly luciferase homologues that can catalyze similar\u0000reactions with similar amino acid sequences. Several applications are described in different publications on bioluminescence\u0000and chemiluminescence. Firefly flicker is always very interesting to observe it. Firefly luciferase emits light but does not\u0000generate heat, which also causes curiosity because it solves the enzyme-catalyzed reaction. Here we mainly review the\u0000structure, mechanism, application, advantages and disadvantages of luciferase.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"140 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124410925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}