Journal of Genetic Engineering and Biotechnology Research最新文献

{"title":"Identification of Bacterial Microbiota in Aqua Cultured Shrimp Penaeus Monodon","authors":"","doi":"10.33140/jgebr.06.01.09","DOIUrl":"https://doi.org/10.33140/jgebr.06.01.09","url":null,"abstract":"The knowledge of bacterial communities in the brackish shrimp farming in Kerala is still insufficient.16S rRNA gene-based highthroughput sequencing revealed distinct and diverse microbial communities in the analyzed sample. Analysis of the results showed a high abundance of Betaproteobacteria, followed by Alpha proteobacteria, Clostridia, Actinobacteria, Gamma proteobacteria and Bacilli in the metagenome retrieved from the gut sample. Unclassified bacteria also contributed a significant portion of the metagenome. Microbes that play essential roles in nutrient cycling and mineralization of organic compounds such as Bacteroidetes, Planctomycetes, Gamma proteobacteria, Firmicutes, Cyanobacteria, and Actinobacteria could also be identified. Due to the strong influence of the gut microbiota on fish health, dominant bacterial species in the gut are strong candidates for probiotics. This study aimed to characterize the gut microbiota of giant tiger shrimp, Penaeus monodon. These findings provide valuable information on the microbial community and contribute to control the diseases in shrimp farms","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140228876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Electrostatic Hotspots at the Binding Interface of Amylin and InsulinDegrading Enzyme: A Structural and Biophysical Investigation","authors":"","doi":"10.33140/jgebr.06.01.08","DOIUrl":"https://doi.org/10.33140/jgebr.06.01.08","url":null,"abstract":"Amylin, also known as islet amyloid polypeptide (IAPP), is a metabolic homeostasis-related hormone that is produced and released by the β cells of the pancreas, the same cells that produce insulin. Insulin-degrading enzyme (IDE) is a protease enzyme that plays an essential role in the breakdown and degradation of various peptides, including insulin and amylin. Direct binding & interaction between amylin and IDE is inextricably linked to the degradation of amylin, and research and development effort in this area is crucial to understand the potential therapeutic implications of disrupting the IDE-amylin interaction in the context of conditions where metabolic homeostasis needs to be regulated, such as diabetes and obesity. Here, this article incorporates currently available experimental complex structure of amylin and IDE, and delves deep into the interstructural biophysics underlying the binding interface of the two interacting partners. With a set of comprehensive structural biophysical analysis, this article identified an intriguing region of high electrostatic potential indicative of strong binding sites between the first N-terminal lysine (Lys1, K1) residue of amylin and Glu341 (E341) of IDE. This unique electrostatic hotspot presented herein paves the way for the rational design of drug-like small molecules that can selectively disrupt this interaction, offering a targeted therapeutic strategy for improved metabolic homeostasis, particularly for patients with diabetes and obesity.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140248209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency and Quality Improvement In Pathology Diagnostic by Using Computational Pathology: Software-Based Analysis of Perineural Invasion in Colon Carcinoma","authors":"","doi":"10.33140/jgebr.05.03.07","DOIUrl":"https://doi.org/10.33140/jgebr.05.03.07","url":null,"abstract":"Introduction: Many pathology laboratories are transitioning from diagnostics with glass slides to diagnostics with whole-slide images (WSI). One of the advantages of digital pathology is the possibility of using the software. Annotations made by software could increase the efficiency and the quality of pathologists´ work. In this research, annotations for nerves were used in WSI´s of colon carcinoma cases to help the detection of perineural invasion to improve the efficiency (time needed to find perineural invasion) and quality (the possibility of finding more perineural invasion in colon carcinoma cases and standardization) of the diagnostics. Materials & Methods: 4 pathologists got 148 colon carcinoma cases without annotations made by software to screen the slides for perineural invasion. After a minimum of 4 weeks of washing out, they got the same instances with annotations for nerves made by software to find perineural invasion. We compared the time they needed for the cases with and without annotations and the percentage of perineural invasion found with and without annotations. Results: All 4 pathologists took advantage of the annotations made by software regarding the time they needed to conclude the perineural invasion, and the difference between the average time of all 4 pathologists without and with the use of software was statistically significant. All 4 pathologists found individual more perineural invasion. However, the average % of found perineural invasion without and with the help of software needed to be more substantial.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"91 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139238988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sero-Prevalence of Hepatitis B Virus and Associated Factors among Pregnant Women’s Attending Antenatal Clinics in Public Hospitals of Hawassa City, Southern, Ethiopia, 2021. Cross Sectional Study","authors":"","doi":"10.33140/jgebr.05.01.08","DOIUrl":"https://doi.org/10.33140/jgebr.05.01.08","url":null,"abstract":"Introduction: Viral hepatitis caused by the hepatitis B virus (HBV) is now recognized as a growing worldwide health issue. A significant global public health issue is the estimated 350 million people who have been diagnosed with chronic HBV infection and the 686,000 deaths that occur each year. Since HBV infected pregnant women are at risk of transmitting virus to their babies, knowing prevalence of HBV and its risky factors in the area is very essential. Providing area-specific hepatitis B burden data to enable health policymakers to develop suitable and cost-effective HBV infection prevention strategies. Objective: The objective of this study was to determine the sero-prevalence of HBV and related factors in pregnant women attending ANC at hospitals in Hawassa City, 2021. Method: From May 20 to July 30/2021, 320 pregnant women were the subjects of a hospital-based cross-sectional research at the Public Hospital in Hawassa City. SPSS version 25.0 was used to enter and analyze the data. HBV prevalence was determined using descriptive statistics; bivariate and multivariate logistic regression analysis was utilized to find independent predictors of HBV status at p-value <0.05. Results: Overall Sero-prevalence of HBV infection was [6.9% (95% CI (6.6%, 7.2%)]. History of transfusion, history of vulvar ulcer, and contact with patients who had jaundice were all independently predictive of HBV in the study area (AOR = 19, 95%CI, (2.1-27.1), AOR = 14.8 (95%CI, 1.12-19.4), and AOR = 12.7.6[12-13.4], respectively). Conclusion: In the studied area, intermediate endemicity of HBV prevalence was found. To decrease HBV endemicity, it is crucial to raise awareness about HBV transmission specially contact history of jaundice individual, vulvar ulcer and conduct early screenings at blood donor sites before transfusion","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129151552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of a Very Sensitive Refractive Index Sensor Based on Waveguide TM Mode Resonance and Design of a Plasmatic Sensor","authors":"","doi":"10.33140/jgebr.05.01.07","DOIUrl":"https://doi.org/10.33140/jgebr.05.01.07","url":null,"abstract":"This study proposes a highly sensitive refractive index sensor based on the TM wavelength resonance excited in a structure of a plasmonic sensor. The results show that the resonance wavelength will shift significantly with increasing and changing the refractive index, which shows that this method can be used to understand the resonance wavelength and refractive index. Based on this approach, the sensing characteristics, including the sensitivity and the figure of merit (FOM) and the Q quality factor, have been investigated, where the sensor sensitivity has reached 1165 nm / RIU. Compared to plasmon surface resonance sensors, which are based on a similar structure, the proposed sensor can achieve a more flexible range of wavelengths and a wider range of refractive index. Therefore, this method has tremendous potential for use in various fields of measurement, such as biochemical analysis and medicine, and can find useful applications in measurement systems and integrated circuits. Also, all the diagrams obtained in this research have been drawn using MATLAB program.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"358 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134172651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection for Suspected Genetically Modified Maize and Soybean Crops in the Selected Places of Ethiopia","authors":"","doi":"10.33140/jgebr.05.01.06","DOIUrl":"https://doi.org/10.33140/jgebr.05.01.06","url":null,"abstract":"Detection of genetically modified organisms (GMOs) in crops is an important issue for all the subjects involved in seed quality control and customer’s right. Due to the increasing number of GMOs research and development activity in the globe during the past few years, it has become necessary to screen and regulate highly practiced GMO crops. In this study soybean and maize crops samples were collected at different agricultural farm lands and commercial super markets that suspected for GMOs. Then, genomic DNA were extracted from crops samples by using Phenol chloroform method. By following ,the extracted genomic DNA amplified by using two oligonucleotide primer sequences such as CaMV35s and Lectin with the help of thermal cycler Polymerase Chain Reaction (PCR). In addition, positive control taxon -specific Invertase and Lectine genes for maize and soybean were used respectively. Finally, PCR amplicons were run on horizontal agarose gel electrophoresis for the confirmation of amplification. After that electrophoresis results were observed under gel documentation system. Invertase and Lectine gene events were detected in all maize and soybean samples, respectively. However, fortunately in all collected samples of maize and soybean in different places of the country the suspected foreign genes were not detected. Finally,this research result concludes that in the studied area soybean and maize crop samples are GMO free.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122661738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of katG and inhA Genes Mutation in Rifampicin-Resistant Mycobacterium Tuberculosis Using Line Probe Assay (LPA) In Kebbi, Nigeria","authors":"","doi":"10.33140/jgebr.05.01.04","DOIUrl":"https://doi.org/10.33140/jgebr.05.01.04","url":null,"abstract":"Elimination of tuberculosis remains a public health menace, due to multidrug resistant strains of Mycobacterium tuberculosis, that are resistant to the first-line anti-tubercular drugs. Consequently, detection of resistant strains depends on early diagnosis. Intervention of molecular techniques is essential to reduce mortality and morbidity rates. This study aimed to detect katG and inhA gene mutations among rifampicin resistant strain of Mycobacterium tuberculosis in Kebbi State. A cross-sectional study was conducted which covered all the five major health zones in Kebbi State. Two hundred and forty (240) acid fast bacilli confirmed sputum samples were recruited and subjected to Gene Xpert testing. Positive sputum samples were later subjected to Line Probe Assay technique. 14 sputum samples were resistant to rifampicin out of the 240 confirmed acid fast bacilli sputum samples while 226 were sensitive to rifampicin. Mutations were found at the frequency of 100% for katG gene (katG/S315T) in all the 14 rifampicin resistant samples (rpoB/S531L) at the different health zones in kebbi state using the line probe assay. The research has also detected inhA gene mutation at a frequency of 100% in all rifampicin resistant samples showing that mutation is associated with multi resistance to isoniazid and rifampicin. This study confirms that resistance due to katG and inhA mutation is a better surrogate of multidrug-resistant tuberculosis among TB patients in Kebbi State compared to the single-marker analysis. Hence, early molecular detection of rifampicin resistant strains will suffice in determining the management of multidrug resistant tuberculosis in Kebbi State.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128564821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Proliferation of Petunia Hybrida Vilm; Worldwide Important Ornamental Plant","authors":"","doi":"10.33140/jgebr.05.01.02","DOIUrl":"https://doi.org/10.33140/jgebr.05.01.02","url":null,"abstract":"This project tries to artificially grow one of the most ornamentally significant Petunia hybrida when it is out of season. Murashige and Skoog Basal Medium (MS Basal Medium) was used for in vitro growth of P. hybrida, supplemented with different Plant Growth Regulators (PGRs). Various physical and chemical factors on in vitro growth of P. hybrida using different explants were analyzed. The type of auxin that proved significant for somatic embryogenesis of P. hybrida Vilm was 2,4-Dichlorophenoxyacetic acid (2.0 mg/L) using the leaf as explant under controlled environmental conditions, i.e., 92% at 23oC with a 5.8 pH and 16 hours photoperiod & 30g/l sucrose in. Friable calli were also developed from leaf, node, and internodal explants on a tissue culture medium (MS basal medium) when supplemented with other PGRs in certain combinations i.e. 2,4-Dichlorophenoxyacetic acid (2.0 mg/L), Benzyl Amino Purine (2.5mg/L), Benzyl Amino Purine + 2,4-Dichlorophenoxyacetic acid (2mg/L +2.5mg/L), Naphthalene Acetic Acid (1.5mg/L), 2,4-Dichlorophenoxyacetic acid + Naphthalene Acetic Acid (2mg/L+1.5mg/L) and Benzyl Amino Purine + Naphthalene Acetic Acid (2.5mg/L+0.5mg/L) at optimal physical factors under strict aseptic environment. These results may enable one to produce the required type of P. hybrida Vilm during off and on seasons on a commercial scale by using plant tissue culture as a tool.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130365243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Presentation and Associated Factors of Lower Limb Chronic Venous Insufficiency at a Tertiary Hospital in Uganda: A Cross-Sectional Study","authors":"","doi":"10.33140/jgebr.04.03.14","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.14","url":null,"abstract":"Background: Lower limb chronic venous insufficiency (CVI) is a common disorder associated with sequelae of changes that lead to varicose veins and skin trophic changes. There is limited data on potential associated factors from representative studies yet its burden and impact on the quality of life is quite significant. We evaluated the clinical presentation and associated factors of lower limb CVI among patients at Mulago National Referral Hospital (MNRH). Methods: We conducted a cross-sectional study of 104 consecutive participants with CVI aged ≥ 18 years attending the outpatient clinics of MNRH. Limbs of participants with history of surgery pertaining to CVI were excluded. We recorded information on demographics, relevant medical history, symptoms, lifestyle and clinical presentation. We described CVI using the clinical and anatomical components of the clinical-etiologic-anatomical and pathophysiologic (CEAP) scale. Data was analyzed at both bivariate and multivariate levels using Stata version 14.1 Results: Altogether, 104 participants were enrolled with a male: female ratio of 2:1, age range of 18 to 85 years and median age of 43 years. The commonest venous symptoms included; venous pain (96.2%), sensation of swelling (93.3%) and heaviness (79.8%). Of the 146 affected limbs, CEAP clinical class included; C0:59.6%, C1:40.4%, C2:80.8%, C3:88.5%, C4:73.1%, C5:30.8%, C6:51.9%. The anatomical class identified superficial veins in 138 (90.5%), deep veins in 46 (29.7%) and perforator veins in 122 (79.7%) affected limbs. Men had significantly higher proportions of skin trophic changes 88.7% vs 63.6% (p=0.003) while females had significantly higher proportions of venous symptoms compared to men 57.6% vs 32.4% (p=0.015). All participants with a smoking history had skin changes. Obese and overweight patients had higher proportions for venous symptoms 13 (54.2%) and 14(50%) respectively as compared to those who had a normal BMI 14(28.6%). Conclusions: Most participants are symptomatic with advanced disease, young and predominantly male. Males had 4.5 times higher odds of having skin changes as compared to females. Although some associated factors such as age, gender are immutable, others can be modified, such as physical activity, cigarette smoking.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117118869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Locked Posterior Shoulder Dislocation with Impression Fracture Treated All Arthroscopically With the Use of an Allograft Bone Block a Case Report","authors":"","doi":"10.33140/jgebr.04.03.12","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.12","url":null,"abstract":"Locked posterior shoulder dislocations are uncommon and poses many difficulties in diagnosis. They are often overlooked during initial examination and delayed diagnosis adversely affects healing process. Apart from many open treatment options there are reports of single attempts to treat such cases arthroscopically. We present an original case of a posterior locked dislocation of the shoulder joint with a fracture of the lesser tuberosity followed by reverse Hill-Sachs fracture, treated in a novel fashion all-arthroscopically with the use of allogenic bone graft. According to Constant Shoulder Score that tries to asses functional and subjective performance of the shoulder joint before the operation and after 12 months, we achieved a leap from 11% to 84%. The patient restored almost full range of motion and painless movement in activi-ties of daily life as well as during sports. The use of an arthroscopy reduces the invasiveness of the procedure, improves visualization of the joint and allows augmentation of the bone loss without performing an open approach. We believe that this is A promising method of treatment for selected cases of locked posterior shoulder dislocation.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115390130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}