Journal of Genetic Engineering and Biotechnology Research最新文献

{"title":"Molecular Docking Analyses of Phytochemicals from African Herbal Plants Exhibit Inhibitory Activity against Therapeutic Targets of Sars-COV-2","authors":"","doi":"10.33140/jgebr.4.03.13","DOIUrl":"https://doi.org/10.33140/jgebr.4.03.13","url":null,"abstract":"Viral diseases remain the leading cause of death due to infectious agent globally. Presently, global public health threat of international concern is the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of coronavirus disease-2019 (COVID-19) of worldwide prevalence. Plants worldwide including plants of African ethno-pharmacological relevance are a natural source of abundant and diverse phytochemicals with bioactivity against microorganisms including viruses. We selected 13 plants used in African traditional medicine for the treatment of viral diseases to screen for phytochemicals capable of interfering with SARS-CoV-2 therapeutic targets using AutoDocking Vina in silico approach. 25 phytochemicals from these plants that passed the Lipinski rule of drug-likeness were assessed for antiviral activity against three SARS-CoV-2 therapeutic targets, namely: spike glycoprotein, Papain-like protease and 3C-like proteinase. The crystal structure of the viral protein targets were obtained from the protein databank website (https://www.rcsb.org/). The active sites of the target proteins were predicted using SCFBio Server (http://www.scfbio-iitd.res.in/dock/ActiveSite.jsp) from the pdb file as input. The antiviral herbal phytochemical compounds were then docked with Papain-like protease, 3C-like proteinase and spike glycoprotein. The Autodocking hit results generated six lead phytochemicals out of the twenty-five (25) phytochemicals obtained from the African traditional herbs with potential anti-SARS-CoV-2 activity. The lead molecules with their binding affinities against Papain-like protease and 3C-Like Proteinase are as follows: Ginsenosides (-9.9), ursolic acid (-9.4), oleanolic acid (-9.4), cynarine(-8.9), glabridin (-8.5) and cinnamoyl-echinadiol (-8.2). We advocate for further in vitro and in vivo studies to evaluate the activity of these lead compounds with a view to optimized drug intervention against COVID-19 pandemic.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128065827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection and Genotyping of P. Multocida, M. Hemolytica, and B. Trehalosi Isolates Targeting the Virulence Associated Genes from Ethiopian Cattle and Sheep","authors":"","doi":"10.33140/jgebr.04.03.11","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.11","url":null,"abstract":"Pasteurellaceae families are usually considered opportunistic pathogens which inhabit normal flora on the mucosal membranes of the upper respiratory and the lower genital tracts of mammals and birds. The majority of P. multocida, M. hemolytica, and B. trehalosi isolates are opportunistic animal pathogens and cause disease only under certain conditions. Some of the target genes are shared by isolates found in the same or different genera.. The alpha / beta hydrolase kmt1 genes part of OMP and SLP are shared by all P.multocida species and FbpA is common gene for both P.multocida and M. hemolytica isolates. Other virulence related genes are used for genotyping of P.multocida, M.hemolytica and B.trehalosi isolates. The objective of the current research was focused on the detection and genotyping of different target genes that can be found in the isolates of P. multocida, M. hemolytica, and B. trehalosi using various PCR primers. The distributions of virulence-associated genes were assessed in the isolates of the three genera. Materials and methods A total of eight isolates from P. multocida, M. hemolytica, and B. trehalosi isolated from clinical cases of cattle and sheep and stored at the Bacteriology laboratory of NVI were used as source of samples for the present study. DNeasy® Blood & Tissue Kit (Qiagen, Germany) was used for the extraction of DNA from bacterial isolates. Different primers were used for genotyping of P. multocida, P.hemolytica and B.trehalosi isolates. Results P.multocida was detected using species-specific kmt1 primer with an amplicon product of 460bp. Four serogroups of P.multocida (A, B, D, &E) were detected using serotype-specific primers with amplification products of 1044bp, 760bp, 657bp, and 511bp respectively. PCR was conducted to HS causing P.multocida type B and detected 620 bp amplicons. SLP genes were detected in all P.multocida serotypes with an amplification product size 1400-1553bp. In addition to that, the four P. multocida serotypes were found to be positive for BRD-PmSLP with an amplification product of 460bp but using recombinant HS-SLP primers resulted in the double band at 460 bp and 541bp for serotypes A and D. However, HS causing type B& E serotypes were detected with an amplified product of 541bp. TbpA2 has been detected with an amplicon size of approximately 750 bp for types (A and D) and 1300bp for type B using TbpA-F2/Rev primers. All of the P.multocida serotypes (A, B, D&E) and M. hemolytica (A: 1) were strongly positive for the iron acquisition FbpA gene with amplification bands at 500bp and 1000bp, respectively. A multiplex PCR as-say was carried out for detection of M. haemolytica A: 1 using PHSSA and Rpt2 primers with amplification products of 327pb and 1022 bp respectively. The three isolates of B. trehalosi were identified using BtsodA primers with the result of 144bp amplicon size. Conclusion From this work, we understood that P.multocida and M. haemolytica shared genetic materials like iron bind","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"424 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133615204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating Combining Ability and Response of Tomato (Solanum Lycopersicum l.) Genotypes to Late Blight (Phytophthora Infestans) Disease","authors":"","doi":"10.33140/jgebr.04.03.03","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.03","url":null,"abstract":"Eight tomato varieties were selected based on all over per se performance and late blight disease reaction. Crosses were made in 8×8 half-diallel mating design to produce 28 F1 single cross hybrids. The experiment was conducted in greenhouse at Shambu campus research center in 2020. Necessary data for late blight disease were recorded. In this study highly significant genotypic differences were observed indicating the existence of genetic variability among the crosses to late blight. Analysis of variance for the combining ability indicated GCA and SCA mean squares were significant at (P < 0.001) for all disease parameters. The ratios of GCA/SCA variances for all disease parameters were less than unity implying the predominance of non-additive gene actions. Among all parental lines, ARP tomato d2 and Metadel were identified as stable sources of resistant genes for late blight disease resistance with positive days of first disease appearance and negative disease severity index and AUDPC values for GCA effects. From the analysis of epidemiological data and disease progress curves the Logistic model (R2=96.5) better described the disease progress curves than the Gompertz model (R2=92.5) indicating the presence of delays in epidemics and the infection point of the late blight. ARP tomato d2 and Metadel were identified as a good general combiners for late blight disease parameters. Thus, these parents were recommended to be used in breeding programs with a purpose of developing late blight resistant single cross hybrids. In conclusion of the study, ARP tomato d2 and Bishola parents could be used as a source of resistance gene and potential late blight resistant single cross hybrids (ARP tomato d2 x Metadel, Fetan x ARP tomatod2, Bishola x ARP tomatod2, Metadel x Miya and Bishola x Metadel) were identified. Therefore, it is recommended that these hybrids can be used for direct production where this disease is the most prevalent and/or for further breeding programs in generating hybrids for future use.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123950077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is the Impact of Light Curing Modes of High-Powered LED on Temperature Rise in Primary Teeth Dentin","authors":"Obada Jabbour, Reem Alfares","doi":"10.33140/jgebr.04.03.01","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.01","url":null,"abstract":"Background: Temperature rise under primary teeth dentin is a severe stress that can cause irreversible damage to the pulp. The objective of this study was to compare the temperature rise under primary teeth dentin induced by a light emitting diode (LED) with different light curing modes. Methods: Sixty dentin discs of 0.5- 1- 1.5- and 2-mm thicknesses were prepared from human primary molars. The resin composite placed in a Teflon cavity was cured using a high-powered LED (Foshan JERRY Medical Apparatus CO., LTD, Foshan, China) for 20 s. The different modes tested in this study were standard mode, ramp mode, and pulse mode (n=5). Temperature was recorded using a k-type thermocouple in direct contact with the dentin disc. Temperature change data were subjected to analysis of variance (ANOVA) and Tukey’s test. Results: The highest temperature rise was observed under a 0.5 mm thick dentin disc with standard mode (4.7 ± 0.42), whereas the lowest values were recorded with pulse mode under 2 mm thick dentin (2.5 ± 0.23). Pulse mode produced a significantly lower temperature rise than standard mode for all dentin thicknesses (P<0.05). Ramp mode gave significantly lower values than standard mode in the 0.5 mm group (P<0.05). For the standard and ramp modes, the 0.5 mm thick group exhibited a higher temperature rise than the 2 mm thick group (P<0.05). Conclusions: Temperature rise related to dentin thickness and curing modes. The standard mode led to a significantly higher temperature rise under thinner dentin than the other modes. Pulse mode gave the lowest values. Thus, it is recommended for use in deep cavities. Clinical Significance: The temperature rise during polymerization of the resin composite with the high-powered LED appeared to be below 5.5 ℃. Hence, it appears to be safe for use in pediatric dentistry.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124641058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Intestinal Parasitic Infection and Associated Factors among Adama Science and Technology University Student Adama Town, Oromia, Ethiopia","authors":"","doi":"10.33140/jgebr.04.03.07","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.07","url":null,"abstract":"Introduction: University students who live together in campuses and share different materials for their day-to-day activities may expose them to intestinal parasitic infections. As far as our knowledge is concerned, there is insufficient information on the magnitude of parasitic infections among students at Adama Science and Technology University Objectives: To determine Prevalence of intestinal parasitic infection and associated factors among Adama University Student Adama Town, Oromia, Ethiopia. Methods: A cross sectional study was conducted among 483 students attending higher health center at Adama University Student by using systematic random sampling technique. Data on socio-demographic characteristics and potential associated factors for Intestinal parasitic infection was collected by structured interviewer administered questionnaire. One-gram stool samples for direct saline microscopy was collected and processed in accordance to the standard parasitological techniques and procedures at Adama Science and Technology University Higher Health Center Laboratory. Data entry and analysis was done using SPSS version 20.0.Chi- square test and multiple logistic regression was used to identify associated factors. Association between variables were considered statistically significant only if a two-sided P-value <0.05 at 95% confidence level. Results: This study demonstrates the overall prevalence of intestinal parasite among student accounts 20.1% (95%CI; 16.8, 23.8). Two protozoa and three helminthes spp were detected from total stool sample examined. The predominate parasite isolated were E.histolotica 63(13.0%) followed by G.lamblia 27(5.6%), Taenia spp 3(0.6%), Hookworm 2(0.4%) and H.nana 2(0.4%) respectively. being rural residence[AOR]: 2.69, 95% CI: (1.22,5.9)], lower parental educational status(illiterate [AOR]: 5.5, 95% CI: (1.99,15.5)] and primary[AOR]: 3.75, 95% CI: (1.5,9.34)], food source from private cafeterias[AOR]: 2.46, 95% CI: (1.15,5.2)], not hand washing before food and after defecation[AOR]: 4.9, 95% CI: (1.96,12.3)], Habit of eating raw vegetables and fruit[AOR]: 4.8, 95% CI:(2.28,10.1)], walking with bare foot[AOR]: 13.2, 95% CI: (5.4,32.4)], not trimming finger nail [AOR]: 6.85, 95% CI: (3.2,14.6)] and not using soap [AOR]: 7.53, 95% CI: (3.2,17.8)]were significantly associated with parasitic infection. Conclusions and Recommendations: The present study demonstrated the frequencies of intestinal parasitic infections among students were found to be high. Residence, educational status of parents, hand washing before meal and after defection, eating raw vegetables and fruit, walking with bare foot was significant predictors of parasitic infection: Hence all students should wash their hand before meal and after defecation, consume cooked vegetables, avoid walking with barefoot and trim their fingernail and university should improve and sustain inspection and food handler screening of private cafeterias.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130233525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Study of the Drying Kinetics of Products with High Water Contentapplication on Green Chilli","authors":"","doi":"10.33140/jgebr.04.03.02","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.02","url":null,"abstract":"The main objective of this work is to determine the graphs of relative humidity loss, and the speed of drying for green pepper, to identify the characteristic parameters influencing the drying of products with high humidity content, and to establish satisfactory models that characterize the drying kinetics. This experimental study was carried out using an indirect type of a solar dryer, which functions in the mode of natural convection, intended for the thermal treatment to medium duration of the agro-alimentary products. In this work, we are interested in the evolution of the temperature and the mass of the material to be dried, whose manipulations took place in May and July of the year 2009","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131270307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Type of Aeronautical Risk Assessment: Performance of Kohonen SelfOrganizing Maps in Identifying Brazilian Aircraft with Greater Associated Risks","authors":"","doi":"10.33140/jgebr.04.03.04","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.04","url":null,"abstract":"The purpose of this paper is to present a new way of assessing aeronautical risk using a configuration of Kohonen Self-Organizing Maps (SOM) to identify the Brazilian aircrafts more likely to be involved in aeronautical accidents and the riskiest Brazilian aircrafts. The technique described is classified as predictive for managing aeronautical risks, according to DOC 9859, and can be used both in the context of prevention and investigation of aeronautical accidents/incidents, as well as in the context of the insurance industry. Using this technique, it was possible to identify the 147 Brazilian aircraft with the highest associated probabilities of occurrence of aeronautical accidents, and the 180 with the highest associated risks. Five years after this identification, the respective percentages of aeronautical accidents/incidents were 34% and 27%. The application of this technique can help achieve the objective of the aeronautical community in determining what, where, and when the next aeronautical accidents and/or incidents will occur. Another aspect of the present work is to demonstrate that data collected by the national civil aviation agency in Brazil can be used to implement a predictive methodology for the management of safety in civil aviation.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130392653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over Expression of Recombinant Staphylokinase and Reduction of Inclusion Bodies Using IPTG as Inducer in E. coli BL21 DE3","authors":"","doi":"10.33140/jgebr.04.03.06","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.06","url":null,"abstract":"A previous research and studies have established the significance of describing Staphylokinase as a thrombolytic treatment, particularly in patients with cardiovascular disease, stroke, and other life-threatening disorders. Staphylokinase plays a significant part in the coagulation process through formation plasmin-Staphylokinase complex on the surface of the clot which activates plasminogen is generated by some strains of Staphylococcus aureus. The publication described the use of the E. coli strain BL12DE3 to produce the protein via the pET21a transporter is predicated on the use of a chemical stimulator (IPTG) which plays an important role in regulating protein recombinant by stimulating protein synthesis. Strain BL12DE3 activates T7 polymerase, encoding LacI via the pET21a transporter. It has been demonstrated that the IPTG inducer achieves the maximum level of protein expression in the shortest amount of time. A Staphylokinase was generated as a soluble protein in excess of 45 % by SDS-PAGE and subsequently purified by chromatography.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132766562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Metabolic Rate, Excretion, Energy Reserves, and Starvation Response of Diploid and Triploid Salvelinus Fontinalis and Diploid O. Mykiss after LongTerm Exposure to Elevated Temperature","authors":"","doi":"10.33140/jgebr.04.03.05","DOIUrl":"https://doi.org/10.33140/jgebr.04.03.05","url":null,"abstract":"Background: In fish culture metabolic rate (oxygen consumption), and excretion of particular and dissolved waste products are important parameters limiting the production capacity and influencing the environment. Data on their changes in response to elevated temperature are important for future management of fish farms. Therefore, these parameters were investigated in diploid (2n) and triploid (3n) Salvelinus fontinalis and 2n Oncorhynchus mykiss exposed to 20°C for 32 d in comparison to fish acclimated to 9 °C. Additionally energy reserves were investigated as important indicators for fish performance. Results: At 20°C routine metabolic rate (RMR) of 2n S. fontinalis decreased with increasing exposure time and after 32 d it did not differ from 9 °C. Also, in O. mykiss RMR decreased with exposure time but remained 60 % higher than at 9°C. In 3n S. fontinalis exposed to 20°C RMR was constantly increased for 120%. For all species/ploidy levels faeces dry weight and phosphorus concentration and quantities of excreted dissolved nitrogen and phosphate did not differ between 9°C and 20°C. Lipid concentration of faeces was decreased at 20°C. With exception of O. mykiss also faeces protein was decreased at 20°C. In all species/ploidy levels liver glycogen and visceral fat was decreased at 20°C, while liver triglycerides and adenylate energy charge were not affected. In 2n and 3n S. Salvelinus temperature related responses to 5-d starvation were investigated. In 2n S. fontinalis RMR decreased in starving fish in comparison to fed ones at 9°C and increased at 20°C. RMR of 3n S. fontinalis did not change. For both ploidy levels, dissolved excretion was decreased due to starvation at 9°C and 20°C. Visceral fat decreased due to starving at 9°C, liver glycogen and triglycerides at 20°C. Conclusion: In Salmonidae species and ploidy dependent types of metabolic thermal compensation occurred after exposure to 20°C. Effects on waste products were minor for all investigated species and ploidy levels. Exposure to 20°C represented an energetic stress situation as the visceral fat and liver glycogen depots were depleted. Consequently, at elevated temperature starving can result in a critical energetic stress situation which might lead to complete exhaustion of energy resources.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116564640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel N7-Methylguanosine (M7g) Methylation-Associated Mirna Signature for Prognostic Value in Patient with Carcinoma of Colon","authors":"Chongyang Wang, Shouji Liu","doi":"10.33140/jgebr.04.02.12","DOIUrl":"https://doi.org/10.33140/jgebr.04.02.12","url":null,"abstract":"Background M7G methylation-associated genes were selected as the main research perspectives in this study, establishing a novel m7G methylation-associated miRNA risk signature for prognostic value in-patient with carcinoma of colon. Methods We identified two genes that are m7G methylation-associated and have been validated by searching in the PubMed database. The m7G methylation-associated miRNAs were screened via estimating the differentially expressed of miRNAs in 457 carcinoma of colon patient samples and 8 matched samples from a database named The Cancer Genome Atlas (TCGA). Selecting Single-factor Cox analyses as well as multi-factor Cox analyses constructed the risk signature of 12 m7G methylation-associated miRNAs. Nomograms was adopted to predict the potential impact of m7G methylation-associated miRNAs on occurrence, development. The functions of DEGs that deserving to be noticed were classified and analyzed by functional enrichment analysis. Disease ontology analysis was used to explore the potential connection various diseases and carcinoma of colon. The outcome of associated analysis about immunocyte or immunological functioning revealed that what role does immunocyte or immunological functioning play in the development of carcinoma of colon. Finally, the relationship between high or low expression of significantly DEGs and immune cells and immune function was analyzed. Results The results of nomogram analysis were as follows that the risk signature might predict the prognosis and the overall cumulative probability of survival (1-,3-,5-year) effectively about patients with carcinoma of colon. Through the analysis of Kaplan-Meier curve outcomes, we can draw a conclusion that depressed expressed of hsa-miR-21-3p might be significantly relevant with decreased survival of patients. What the analyses of Gene ontology (GO) , Kyoto Encyclopedia of Genes and Genomes (KEGG) and Disease ontology(DO),taking advantage of the common DEGs about risk score and immune score, demonstrated that these common DEGs play a partial role in the occurrence and development of tumors. Through disease ontology analysis, we found that eating disorder might be associated with carcinoma of colon. Conclusions M7G methylation-associated miRNAs were related to the prognosis of patients with carcinoma of colon. A risk signature established based on these miRNAs could effectively predict the survival rate of later period in patients with carcinoma of colon .According to the displayed outcome from Our research that m7G methylation-associated miRNA regulators can serve as reliable prognostic biomarkers of carcinoma of colon, which might Greater probably be applied as potential targets of therapeutic strategies.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"127 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129852491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}