Journal of Genetic Engineering and Biotechnology Research最新文献

{"title":"PEAR1 Genetic Variants in Essential Thrombocythemia: The Prevalence and Association with Hematological Parameters and ET Mutations","authors":"","doi":"10.33140/jgebr.04.01.02","DOIUrl":"https://doi.org/10.33140/jgebr.04.01.02","url":null,"abstract":"Objective: Essential thrombocythemia (ET) is a type of myeloproliferative neoplasm characterized by the expansion of the megakaryocytic/platelet line. Given the undeniable role of genetic variations in the pathogenesis of ET, as well as the proven effects of PEAR1 SNPs on platelet function, the innovative purpose of this study is to investigate the prevalence of PEAR1 variants (rs12041331 and rs12566888)and their relationship to hematological parameters and ET-related mutations. Materials and Methods: We studied 105 ET patients and analyzed ET patients’ mutational profiles, including JAK2 V617F mutation (detected by Allele-specific PCR), CALR, and MPL mutations (both through PCR amplification). Two SNPs of the PEAR1 gene were assessed through ARMS-PCR, and the Sanger method was used for the validation of ARMS-PCR amplification. Results: The prevalence of rs12041331 and rs12566888 in ET patients were 43.9% and 38.5%, respectively, and rs12041331 was significantly associated with increased platelet counts (P-Value: 0.02). As expected, the incidence of thrombotic events in JAK2+ patients was high and significantly associated with JAK2 mutation (P-Value: 0.02). The prevalence of thrombotic events was also high in patients with the rs12041331 variant. Besides, a significant relationship was also found between the rs12041331 and CALR mutation (P-Value: 0.03). Conclusions: In recent years, the footprint of the PEAR1 variant’s effect on platelet aggregation led to evaluating these variants in ET patients. Finally, the significant relationship between the rs12041331 variant and increased platelet count and CALR mutation announced that the idea of this study could be pursued and challenged in the future.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121793586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergism and Mutual Collaboration between formal Conventional Medicine and Holistic Medicines Including Homeopathy: An Inevitable Essentiality for Oncoming Sensitive Situations","authors":"","doi":"10.33140/jgebr.04.01.01","DOIUrl":"https://doi.org/10.33140/jgebr.04.01.01","url":null,"abstract":"Background: In general, the subject and treatment of diseases are one of the crucial issues that have always occupied the human mind since ancient times. Main body: For this reason, there are various medical schools in different countries and regions around the world. On the other hand, it should be admitted that formal conventional medicine, especially in the treatment of common infectious diseases, has achieved remarkable success in many cases, which cannot be denied. Global eradication of human smallpox, human plague, and polio in many countries worldwide are successful examples in this regard. In veterinary medicine, FMD and brucellosis, which is a common disease between humans and animals, as well as Glanders, have been successfully eradicated in a significant number of developed countries, and bovine plague (Rinderpest) in some Middle Eastern countries such as Iran. The role of vaccination in controlling influenza in humans and birds, and more recently the Covid 19 pandemic, is undeniable despite the fundamental part of health practices such as adequate indoor ventilation. However, the inability of conventional medicine to treat some chronic diseases and the ability of traditional holistic medicine to satisfy a significant number of patients, as well as issues such as microbial resistance and drug side effects, has led to the continuation of traditional methods. Conclusion: As a result, it seems reasonable to look for a framework for the mutual cooperation and synergy of formal conventional medicine and traditional medicines in the sensitive conditions of the world ahead so that the parties can maximize their capabilities in treating human and animal diseases without increasing tolls and costs.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133031507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Variation Between RT-PCR Detected Rotavirus Infection of Naturally Diarrheic Neonatal Calves and Rotavirus Strains of Commercial Vaccines","authors":"A. Zaitoun, A. Abdel-Rady, Zainab M. A. Youssef","doi":"10.21203/rs.3.rs-1059498/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-1059498/v1","url":null,"abstract":"\u0000 Neonatal diarrhea is the main cause of morbidity and mortality in calves, and Rotavirus is the main viral etiology. Rotavirus vaccines are one of the main important methods for control of diarrhea in neonates' calves. In the current study, Deoxyribonucleic acid (DNA) sequencing and phylogenetic analysis of Bovine Rotavirus Group A (BRVA) were performed in our study. 1 Calf guard® vaccine genotype (G6P1) and 5 different field genotypes (2 G6P5, 1 G10P5, G10P? and 1 G10P11) were subjected to DNA sequencing. We observed that at the nucleotide level, G10P5 and G10P? sequences were 100 % identical with each other, two G6P5 sequences were 100% identical with each other and there was no significant similarity between sequences of G10P11 with sequences of G6P5, G10P5, and G10P? The phylogenetic analysis of G10P5 and G10P? isolates showed a close cluster with G10 isolates of Sharkia governorate, Egypt, phylogenetic analysis of two G6P5 and one G10P11 isolate showed a close cluster with the VP4 gene of Rotavirus isolates of Dakahlia governorate, Egypt. Molecular comparison between detected and typed Rotaviruses' genotypes with other genotypes of common vaccines indicated that there were genetically close or distance between field and vaccine Rotavirus strains.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122579482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Modelling of nsSNPs in GABRA2 Gene in Epilepsy and Study of Their Impact On Structure and Stability of GABRA2 Protein","authors":"Z. Agha, M. Azam, Bushra Faryal","doi":"10.21203/rs.3.rs-1019527/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-1019527/v1","url":null,"abstract":"\u0000 Epilepsy is a neurological condition characterized by abrupt, unprovoked, and recurrent seizures that are unpredictable in frequency. The objective of this analysis was to identify novel non-synonymous polymorphisms in the GABRA2 gene and determine their effect on protein structure and stability. Most pathogenic/deleterious nsSNPs were predicted using six different bioinformatic tools. Mutpred2, Mupro was used to check the impact of identified nsSNPS on protein structure and stability. The pathogenic score of SNPs was predicted using the FATHMM tool. The CONSURF webserver was used for conservation analysis of pathogenic SNPs.3-D structure of the protein was realized using SWISS-MODEL and residue position in protein visualized by Lite Mol. GeneMANIA and STRING databases were used to predict the function of interlinked gene interactions. Out of 228 nsSNPs retrieved from the dbSNP database, we identified a novel missense variant, F285S (rs41310789), as most deleterious and its possible association with epilepsy syndrome focal epilepsy. Stability and conservation analysis results interpret rs41310789 present in evolutionarily conserved regions of a gene and affect its structure. This analysis provides information regarding the impact of nsSNPs that might affect the structure and activity of GABRA2 protein. Thus, these coding variants should be taken into scrutiny while genetic screening of epileptic patients.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128452036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol/Water Separation by Linda Type A Membrane","authors":"Zhu Zheng, Yan Li","doi":"10.21203/rs.3.rs-964893/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-964893/v1","url":null,"abstract":"\u0000 Ethanol / Water Separation Technology is a long term research. LTA molecular sieve, which is an industrially important desiccant as a kind of zeolite molecular sieve. It is always used in the reverse osmosis method, which is one of the sea water desalination, and extraction of industrial ethanol method in the world. Membrane separation technology is widely used in cell science[1, 2], human science[1, 2], microbial science[3], military industry, battery technology[4, 5] and so on. With 2 years invention and attempts, prototype membrane is finished and the filtered data has also been measured in different side, such as Linda Type A(LTA) making temperature, raw material ratio of membrane preparation and filter curves. The physicochemical analysis of the samples before and after filtration was carried out. The manbrane filtration losses are very low and can be used with food grade filtration","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120940529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heritability and Genetic Advance as Genetic Indicators for Improvement in Two Cotton Crosses","authors":"","doi":"10.33140/igebr.03.02.03","DOIUrl":"https://doi.org/10.33140/igebr.03.02.03","url":null,"abstract":"The primary breeding goal for the Egyptian cotton breeding program is how to genetically improve both yield and fibre quality traits, while most of these agronomically traits are biometric traits. The objective of the present study is to evaluate variability and estimate frequency of transgressive segregants in order to isolate early superior individual plants which exceeding the better parent for some yield and fiber quality traits in two intraspecific cotton crosses in early transgressive segregating generation (F2 ). The F1 was highly significant superior than the better parent (Giza 97 and Giza 94) of the two crosses for all the studied traits except boll weight and lint %. Most of the studied traits in F2 generation showed high values of broad sense heritability coupled with low GAM % indicated that these traits controlled by non-additive gene action. All the studied traits had positive skewness sign except for lint %. Presley index and uniformity index for the two cotton crosses and upper half mean for cross II showed negative skewness. The negative skewness indicated that the population had more plants frequency with higher mean values than population mean and controlled by dominancy alleles. While, the traits that had positive skewness are controlled by additive gene action. The two cotton crosses showed transgressive segregants for all the studied traits. Cross I has higher transgressive index for yield traits than cross II, while cross II has the same trend for fiber quality traits than cross I. These results indicated that the both parents of the two cotton crosses had different alleles and genes governing the respective studied traits, which will help cotton breeder to combine beneficial alleles into a single genotype by rigorous selection process. This strategy could be used to improve many economic biometric traits by using better and stringent selection procedure to enhance Egyptian cotton productivity which is major concern in Egypt. The breeder can use transgressive segregation as an indicator of genetic variability to select the most superior plants.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132764342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of the Influence of Extracellular Matrix Biomimetics on the Viability and Insulin-Producing Function of Isolated Pancreatic Islets","authors":"V. Sevastianov, L. A. Kirsanova, A. S. Ponomareva, S. Gautier","doi":"10.33140/igebr.03.02.02","DOIUrl":"https://doi.org/10.33140/igebr.03.02.02","url":null,"abstract":"The creation of a pancreas tissue-engineered construct based on isolated pancreatic islets is hindered by problems associated with maintaining their viability and insulin-producing function. Both biopolymer and tissue-specific scaffolds can contribute to the maintenance of the structure and function of pancreatic islets in vitro and in vivo. A comparative morphofunctional analysis in vitro of isolated pancreatic islets cultured with a biopolymer collagen-containing scaffold and a tissue-specific scaffold obtained as a result of pancreatic decellularization was performed. The results showed that the use of the scaffolds contributes not only to the maintenance of the cultured islets viability, but also to the prolongation of their insulin-producing functions, compared to the islets monoculture in vitro. A significant increase was found in basal and stimulated (under glucose loading) insulin secreted by the islets cultured with the scaffolds. At the same time, the advantage of using a tissue-specific scaffold in comparison with a biopolymer collagen-containing scaffold was shown. We think that these studies will become a platform for creating a human pancreas tissue-engineered design for the treatment of type 1 diabetes.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125101936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Sequences Variations of the E768D Mutation Found in Familial Medullary Thyroid Carcinomas","authors":"","doi":"10.33140/igebr.03.02.04","DOIUrl":"https://doi.org/10.33140/igebr.03.02.04","url":null,"abstract":"Medullary thyroid cancer or MTC is present in sporadic form (75% of cases) and in familial form (25% of cases), in this latter situation, the MTC is a part of Multiple Endocrine Neoplasia type 2 (MEN 2). The MEN2 is divided into MEN2A, MEN2B and FMTC or isolated familial MTC. The MEN2 are rare hereditary disease, transmitted as an autosomal dominant mode, linked to mutations of the RET gene. The discovery of a mutation in the RET proto-oncogene by molecular biology techniques in a index case of MTC confirms the diagnosis of familial and allows the genetic testing of healthy clinically related index case: those who carry the genetic mutation, will be offered prophylactic thyroidectomy before any biological or clinical manifestation. The genetic analysis of the RET gene was performed by PCR / sequencing. The E768D mutation was found in the exon 13 of the RET gene in 2 differences sequences forms (GAG/GAC et GAG/GAT). This mutation, already described, found in the FMTC.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133683545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Survey of the Combinatorial Paraphernalia in Protein Synthesis","authors":"","doi":"10.33140/igebr.03.02.05","DOIUrl":"https://doi.org/10.33140/igebr.03.02.05","url":null,"abstract":"Statement of the Problem: The combinatorial paraphernalia in protein synthesis to be surveyed are multifarious, embracing, phenomena, processes, activities and materials, all characterized by plurality and dissimilarity. The materials usable are phenomenal and must be a set of discrete plural and dissimilar objects, e.g. the RNA four bases of Adenine, Uracil, Guanine, Cytosine (A,U,G,C) for the activity of permutation for building genetic code. Sequences for protein type sequence composition, proliferation and diversification as inherent in protein synthesis. Methodology and Theoretical Orientation: We are in for combinatorics which is the scientific study of the phenomenon of input/output productivity exhibited by a duality of numeral entities as in permutation of specified set (n) of dissimilar discrete plural. Things and selection (r) of them. The Dalina apparatus of Input/Output Multiplicative Replication system equipped with Square Kinematics View Mixing Technique sourced from inchoate Numeration Science literature being developed by this author is in use for the computation of 4 from 4 permutations of RNA four bases, A,U,G,C constituting the 24 quadruplet genetic code as the workforce in protein synthesis. Findings: The combinatorial paraphernalia in protein synthesis identified and surveyed comprise 14 characteristics, 3 materials and 11 processes/operatives. Conclusion and Significance: The relevance of the several identified and surveyed combinatorial paraphernalia in protein synthesis has been demonstrated by the test of agreeability with the working of the Dalina apparatus of Input/ Output Multiplicative Replication Combinatorial System using the Square Kinematics View Mixing technique for the computation of permutations of RNA four bases A,U,G,C making up the 24 quadruplet genetic code as the workforce in protein synthesis for the substance of all plants and animals throughout CREATION.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"328 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133809933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Genetic Diversity Among Intra- and Interspecific Lowland Rice using Morpho-agronomic Traits and SSR Markers","authors":"A. Ogunbayo, M. Sié, G. Gregorio, D. K. Ojo, K. Sanni, S. Akinyosoye, S. Afeez, Moses Gbenga Akinwale, Najimu Adeniyi Adetoro, J. Adetumbi, O. Amusa, M. Adekoya, S. Obukosia, O. Akinbo","doi":"10.21203/rs.3.rs-816797/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-816797/v1","url":null,"abstract":"\u0000 Rice is staple food in many countries of Africa and a major part of the diet in many others. However, Africa’s demand for rice exceeds production with the deficit of 40% being imported. One way to improve Africa’s rice production is through breeding high yielding varieties suitable for the different environment conditions. This study was conducted to assess the genetic variability and stability performance of 48 lowland rice genotypes including 37 interspecific (Oryza glaberrima × Oryza sativa ssp. indica) and 11 intraspecific (O. sativa ssp. indica × O. sativa ssp. indica) in 12 environments in Nigeria, Benin Republic and Togo using Additive Main Effect and Multiplicative Interaction (AMMI) and Genotype+ Genotype x Environment (GGE) biplot models. The combined analysis of variance revealed significant differences (P<0.01) among the genotypes, environments, and genotypes x environment interaction. Both the AMMI and GGE models identified NERICA-L8 and NERICA-LI2 as the best genotypes for cultivation across environments. Ouedeme environments in Benin Republic were the most stable and ideal for rice cultivation, while Ibadan sites were the most unstable. TOG 5681 had the least yield and was the most unstable across seasons. Genetic diversity was analyzed using 22 important morpho-agronomic traits and 50 simple sequence repeat (SSR) markers and the results were subjected to principal components analysis (PCA). The results revealed that the first eight PC axes (PC1–8) accounted for 75.13% of the total variation, while PC1–4 accounted for 50.39% of the total variation among rice genotypes. However, 10 of the 50 SSR markers were polymorphic and generated 49 alleles (average = 4.9 alleles per locus), suggesting moderate to substantial genetic diversity among the rice genotypes. The polymorphic information content (PIC) ranged from 0.24 to 0.65, with an average PIC value of 0.45. Two structured populations were observed which clustered into five heterotic groups and an outgroup, respectively. This suggests that heterosis could be exploited in the next hybridization program by crossing one of the genotypes in any SSR marker-defined cluster, with the rice accession TOG 5681 in cluster I. The results of this study suggest that morpho-agronomic traits should be used to compliment SSR data in rice diversity studies, especially if a few polymorphic SSR markers are to be used.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127752181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}