Veterinary immunology and immunopathology最新文献

{"title":"Mucoadhesive chitosan-based nano vaccine as promising immersion vaccine against Edwardsiella tarda challenge in Nile tilapia (Oreochromis niloticus)","authors":"Nandhakumar ,&nbsp;Ishwarya Ramachandran ,&nbsp;Preetham Elumalai","doi":"10.1016/j.vetimm.2025.110976","DOIUrl":"10.1016/j.vetimm.2025.110976","url":null,"abstract":"<div><div>Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce a strong mucosal immune response against Edwardsiellosis, a serious bacterial disease in Nile tilapia caused by <em>Edwardsiella tarda</em>. This study aims to develop and investigate the efficacy of immersion delivery using a chitosan nano-vaccine (CS) with β-glucan (BG) as an adjuvant to enhance the mucoadhesive properties of the vaccine. The prepared vaccines were nano-sized and spherical as confirmed by scanning electron microscope (SEM), and the images show that nano vaccine greatly increased the binding and penetrating ability into gills when compared with formalin-killed vaccine. Fingerling-sized Nile tilapia (12 ± 2 g) were randomly assigned to four groups: control, Formalin Killed vaccine (Positive control), Chitosan/β-glucan alone (CS/BG), and Chitosan/β-glucan Vaccine (CS/BG V). Fish received immersion baths on days 1 and 21 with a 30-min booster dose. Samples were collected at two time intervals (14, and 28 days post-vaccination (<em>Dpv</em>)) to evaluate innate immune responses through lysozyme, myeloperoxidase, NBT, and superoxide dismutase activity were significantly elevated in vaccinated fish compared to the control group (<em>p </em>&lt; 0.05). IgM antibody titers, measured by ELISA, peaked at 14 and 28<em>Dpv</em> compared to the non-vaccinated group. Furthermore, after vaccination, gene expression analysis using qRT-PCR showed a significant increase <em>in IgM, TNF-α, IL-1β, TCR- β, MHC-I, and IL-8</em> in the spleen of CS/BG V fishes, with similar antibody responses observed. The efficacy of the vaccine was further assessed by challenging the fish with virulent <em>E. tarda</em> after 36 <em>Dpv</em>, and observed 15 days for cumulative mortality. The results demonstrate that the vaccine showed significant protection of 24.44 %, 37.78 %, 48.89 % and 68.89 % respectively, in groups and high relative percentage survival (RPS) in the C/BG V group compared to the control group. Histopathological examinations of head kidney, spleen, and gills were performed for all four groups showed mild infiltrations. This chitosan nano formulation, adjuvanted with a β-glucan immersion vaccine delivery method, will prove effective for Nile tilapia (<em>Oreochromis niloticus</em>), significantly impacting aquaculture and potentially being applicable against other pathogens in global aquaculture systems.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"286 ","pages":"Article 110976"},"PeriodicalIF":1.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of serological assays for detecting antibodies against structural proteins elicited by foot-and-mouth disease virus vaccines of serotypes O, A, Asia 1 and SAT 2","authors":"Romina Scian ,&nbsp;Maria Pilar Mejías ,&nbsp;Cecilia Caldevilla ,&nbsp;Sabrina Cardillo ,&nbsp;Viviana Malirat ,&nbsp;Ingrid E. Bergmann","doi":"10.1016/j.vetimm.2025.110978","DOIUrl":"10.1016/j.vetimm.2025.110978","url":null,"abstract":"<div><div>Vaccination is the most effective strategy to control and prevent foot-and-mouth disease (FMD). Its effectiveness may be influenced by factors like vaccine formulation, vaccine potency, vaccination strategies, and by circulating variants, which may lead to changes in vaccine requirements and in their control. Serological assays detecting antibodies against structural proteins, such as virus neutralization test (VNT) and structural-protein ELISAs, are widely used to monitor vaccine effectiveness. In overall, serotype-specific tests are required, which should include antigens capable of detecting all variants within a given serotype, particularly those included in the vaccines. This study evaluates the performance of four commercial ELISA kits to detect FMD virus-specific antibody responses in vaccinated cattle and pigs. ELISA results were compared to VNT considered the reference standard. A total of 417 cattle and 189 pig serum samples from animals vaccinated with various formulations, containing O₁ Campos, A24 Cruzeiro, A2001, Asia 1 2015 and SAT 2 2015 strains, were analyzed, along with 50 bovine and 47 pig sera from unvaccinated animals. Analysis of 4170 assays revealed varying ELISA sensitivities across different test formats, influenced by both host species and serotype. Kits demonstrating satisfactory performance and strong correlation with VNT, as measured by Cohen´s kappa coefficient, were identified as potential alternatives to VNT. As expected, tests with antigens homologous to the vaccine strain demonstrated almost perfect agreement with VNT. Nevertheless, some kits which use heterologous, or presumably heterologous, antigens also exhibited very good performances, with kappa values indicating almost perfect to substantial agreement with VNT. All assays showed high specificity, opening the possibility of improving the performance of low-sensitivity kits by adjusting cutoff values. It is highlighted the relevance of using kits with proper validation to ensure the ability to recognize antibodies generated by the vaccines in use.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"286 ","pages":"Article 110978"},"PeriodicalIF":1.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attenuated but not virulent pseudorabies virus activates porcine bone marrow-derived dendritic cells","authors":"Emily C. Ashley ,&nbsp;Walter Fuchs ,&nbsp;Barbara G. Klupp ,&nbsp;Dirk Werling ,&nbsp;Simon P. Graham ,&nbsp;Jane C. Edwards","doi":"10.1016/j.vetimm.2025.110960","DOIUrl":"10.1016/j.vetimm.2025.110960","url":null,"abstract":"<div><div>Pseudorabies viruses (PrV), the causative agent of Aujeszky’s disease, continues to cause economic losses to pig producers across Southeast Asia. PrV is controlled by vaccination with live attenuated vaccines, such as the Bartha K61 strain, which has also shown promise as a viral vector. Despite the success of live attenuated PrV vaccines and their utility to be engineered as vaccine vectors, studies to understand the basis of their immunogenicity are scarce. Here, porcine bone marrow-derived dendritic cells (BMDC) were differentiated by culture with FLT3-L, generating eight myeloid cell populations differing in CADM1, CD172a, CD14, CD163 and CD11c expression, and included CADM1^high conventional (c)DC and CD14⁺ DC. In vitro infection of BMDC with GFP-expressing PrV strains Bartha K61 and virulent Kaplan revealed a more rapid infection with Bartha K61. Compared to PrV Kaplan infection, there was also an increase in maturation marker expression (MHC class II and CD80/86) in both infected and bystander BMDC populations following Bartha K61 infection. This was accompanied by a concomitant increased cytokine response. IL-12 and TNF production associated with the cDC and CD14⁺ DC subsets, suggests that infection of these cells may be key to the potent immunogenicity associated with PrV Bartha K61 vaccination.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110960"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of 1,25-dihydroxy vitamin D3 on inflammation, antimicrobial peptide, and D-dimer levels in Escherichia coli-induced sepsis in neonatal calves","authors":"Emre Eren ,&nbsp;Mustafa Sinan Aktaş","doi":"10.1016/j.vetimm.2025.110963","DOIUrl":"10.1016/j.vetimm.2025.110963","url":null,"abstract":"<div><div>This study evaluated the immunomodulatory, antimicrobial, and anticoagulant effects of 1.25-dihydroxy vitamin D3 in neonatal calves with Escherichia coli-induced sepsis. Thirty neonatal Simmental calves were assigned to three groups: Control (<em>n</em> = 10), Medical Treatment (MT; <em>n</em> = 10), and Medical Treatment plus vitamin D₃ (MT + D₃; <em>n</em> = 10). The MT group received standard sepsis therapy, while the MT + D₃ group was additionally administered 20,000 IU/kg intramuscular vitamin D₃. Blood samples were collected on days 0, 1, 3, and 5 to analyze inflammatory cytokines (NF-κB, TNF-α, IL-1β, IL-10), cathelicidin, D-dimer, iron levels, and hematological parameters. Biochemical indicators including liver, kidney, and heart function, as well as calcium and vitamin D₃ levels, were assessed on days 0 and 5. The MT + D₃ group showed significant clinical and laboratory improvements compared to the MT group. Notably, SpO₂ levels increased, and metabolic acidosis resolved earlier. Hematological findings indicated reduced sepsis-associated anemia, with better preservation of RBC, HGB, and HCT levels. Inflammatory cytokines (NF-κB, TNF-α, IL-1β) significantly decreased, and IL-10 levels were more effectively regulated. Lower D-dimer levels indicated improved coagulation balance. Although cathelicidin levels initially increased, their subsequent decline by day 5 suggested controlled innate immune activation. In conclusion, vitamin D₃ supplementation in combination with standard treatment effectively reduced systemic inflammation, supported innate immunity, and improved coagulation in neonatal calves with sepsis. These results suggest that vitamin D₃ may serve as a beneficial adjunct therapy in bovine neonatal sepsis. Further research is needed to determine optimal dosing strategies and long-term clinical benefits.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110963"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperoside improves intestinal mucosal immunity against zearalenone-induced intestinal barrier damage by regulating intestinal flora","authors":"Tianyu Han ,&nbsp;Yan Jiang ,&nbsp;Zhijun Liu ,&nbsp;Lulu Wang ,&nbsp;Yiding Liu ,&nbsp;Shanshan Fei ,&nbsp;Yu Yang ,&nbsp;Tong Wang ,&nbsp;Baiwen Guan ,&nbsp;Mengran Cui ,&nbsp;Qi Zhang ,&nbsp;Haibin Wang ,&nbsp;Guangliang Shi","doi":"10.1016/j.vetimm.2025.110949","DOIUrl":"10.1016/j.vetimm.2025.110949","url":null,"abstract":"<div><div>Zearalenone (ZEA) is a mycotoxin that is immunotoxic and causes intestinal damage. Hyperoside (HYP) is a natural flavonol side with a wide range of sources and has a variety of pharmacological effects. The aim of this study is to investigate the effect and mechanism of HYP on ΖΕΑ-induced intestinal immunosuppression and intestinal injury in piglets. Histological and ultrastructural changes in the ileum of piglets were observed by H&amp;E staining and transmission electron microscopy. The changes of intestinal microorganisms in the ileum of piglets were detected by 16S rRNA technology. Intestinal chemical barriers (MUC-1 and MUC-2), and physical barriers (β-Catenin, TJ-3, TJ-2, MYLK, Claudin2, and Claudin3) were measured by qRT-PCR. The intestinal immune barrier (sIg A) was detected by Elisa. Immune-related cytokines (TLR-4, IL-1β, IFN-γ, IL-18, IL-6, IL-17, IL-8, IL-25, and TNF-α) were detected by qRT-PCR. The content of ZEA in serum and ileum tissue was detected by Elisa. WB and qRT-PCR were used to detect ferroptosis related indicators (SLC7A11, Gpx4, FTH1, PTGS2, and ACSL4). Our results showed that HYP attenuated ZEA-induced tissue and ultrastructure damage and restored the richness and diversity of intestinal flora in the ileum of piglets. In addition, HYP also alleviated the accumulation of ZEA in the intestine and serum by restoring the chemical, physical and immunological barriers of the ileum. Moreover, HYP was found to attenuate ZEA-induced intestinal ferroptosis. Taken together, our study suggests that HYP can be used as an effective strategy to mitigate ZEA exposure-induced intestinal barrier damage and immune suppression in piglets.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110949"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and identification of porcine monoclonal antibodies against PEDV from single B cells","authors":"Xuan-ang Wang ,&nbsp;Hong-xuan Li ,&nbsp;Lan-Lan Zheng ,&nbsp;Shi-jie Ma ,&nbsp;Ping-Li Wang ,&nbsp;Li Zhao ,&nbsp;Hong-Ying Chen","doi":"10.1016/j.vetimm.2025.110951","DOIUrl":"10.1016/j.vetimm.2025.110951","url":null,"abstract":"<div><div>Porcine epidemic diarrhea virus (PEDV) is a swine enteropathogenic coronavirus causing severe diarrhea and high mortality in neonatal piglets. Pigs of all ages are susceptible to PEDV, and the humoral immune response plays an important role in preventing PEDV infection. However, there is little information on monoclonal antibodies (mAbs) against PEDV derived from single B cells of pigs. In this study, we aimed to develop mAbs using antigen-specific single B cells from peripheral blood mononuclear cells (PBMCs) of pigs via fluorescence-activated cell sorting (FACS). Subsequently, the variable region genes of pig-derived mAbs were amplified and cloned into the plasmid pcDNA3.4 bearing the constant region gene of porcine-derived antibody. Pig-derived mAbs were expressed by transfecting the resultant antibody plasmids into HEK293F cells and validated using indirect Enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), and Western blotting. The results showed 60 double-positive (antigen⁺ and IgG⁺) single B cells were obtained by flow sorting, of which 36 were positive for PEDV and 24 were positive for the N protein of PEDV. A total of 21 mAbs were expressed and purified. Indirect ELISA results showed that 20 bound specifically to PEDV, 19 recognized the N protein, and none reacted with S1D protein. Seven mAbs reacted with PEDV HN2021, as revealed by IFA. Western blotting showed that three N protein-specific mAbs identified linear epitopes, while the remaining 16 N protein-specific mAbs may recognize conformational epitopes. This study laid a foundation for the structural analysis of PEDV and the development of diagnostic reagents and antiviral drug.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110951"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does lipopolysaccharide morphology (smooth or rough) of Brucella abortus vaccine strains influence the potency or efficacy of the vaccine?","authors":"Maysa Serpa Gonçalves,&nbsp;Elaine Maria Seles Dorneles","doi":"10.1016/j.vetimm.2025.110950","DOIUrl":"10.1016/j.vetimm.2025.110950","url":null,"abstract":"<div><div><em>Brucella abortus</em> exhibits the dissociation phenomenon, in which naturally smooth samples lose the O chain of lipopolysaccharide (LPS) and become rough, associated with changes in colony shape, culture characteristics, cell morphology, immunological reactions, biochemical reactions and, possibly, virulence. However, the significance and impact of S-R dissociation in cultures (<em>in vitro</em>) or even <em>in vivo</em> is unclear, especially considering that rough samples have already been isolated from clinical samples in different hosts and, also, are successfully used as vaccine strains. Thus, the objective of this study was to review the literature on <em>Brucella</em> spp. LPS to better understand the impact of the LPS morphology in <em>B. abortus</em> in the vaccinal efficacy. The available information indicates that is undeniable that LPS is related to virulence modulation and inducing immunity in the natural hosts of <em>Brucella</em> spp. However, the continuous emergence of rough variants <em>in vivo</em> (infection) or <em>in vitro</em> (cultivation of the microorganism) suggests that this phenotype is part of the biology of the agent and may confer some survival advantage to the bacteria. In fact, for some samples, the permanent or temporary loss of the O chain (O-PS), whether natural or induced, did not necessarily imply a decrease in virulence, immunogenicity, or post-challenge induced protection, since results in both directions were observed in the literature, depending mainly on the parental samples used and the silenced genes. Thus, it is concluded that the emergence of variants related to the smooth/rough LPS of a sample of <em>B. abortus</em> does not necessarily imply changes in the virulence/immunogenicity of that sample and, consequently, in vaccine potency or efficacy, in case of vaccine strains.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110950"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological diagnosis and post-treatment monitoring of Echinococcus granulosus in experimentally infected dogs using crude and recombinant fibronectin antigens","authors":"Figen Celik ,&nbsp;Muhammed Ahmed Selcuk ,&nbsp;Muhammet Uslug ,&nbsp;Sami Simsek","doi":"10.1016/j.vetimm.2025.110962","DOIUrl":"10.1016/j.vetimm.2025.110962","url":null,"abstract":"<div><div><em>Echinococcus granulosus</em> is a zoonotic helminth responsible for cystic echinococcosis, a significant public health concern. The diagnosis of <em>E. granulosus</em> infections in definitive hosts, such as dogs, is challenging due to the absence of clinical signs. This study aimed to evaluate the diagnostic performance of crude (EgSCA) and recombinant (rEgFN162) antigens for the detection of <em>E. granulosus</em> infection in dogs using ELISA and Western blot assays. Additionally, it sought to identify the most suitable antigen and method for population-based screening and post-treatment monitoring. Adult <em>E. granulosus</em> parasites were collected from experimentally infected dogs using arecoline hydrobromide purgation. Soluble crude antigen (EgSCA) was prepared through freeze-thaw cycles, sonication, and filtration, while recombinant fibronectin protein (rEgFN162) was obtained via gene cloning, expression, and purification in <em>E. coli</em>. The antigenic properties of EgSCA and rEgFN162 were analyzed by SDS-PAGE and Western blot. ELISA assays were performed to assess IgG and IgM responses in experimentally infected and treated dogs. Based on IgG ELISA results, EgSCA showed a sensitivity of 96.66 % and specificity of 66.66 %, while rEgFN162 demonstrated a sensitivity of 76.66 % and specificity of 46.66 %. In Western blot analysis, EgSCA achieved a sensitivity of 90 % and specificity of 83.33 %, whereas rEgFN162 showed 66.66 % sensitivity and 73.33 % specificity. The recombinant antigen showed a higher ability to differentiate <em>E. granulosus</em> infections from other helminth infections. The findings suggest that rEgFN162 is a promising candidate for the serodiagnosis of <em>E. granulosus</em> in dogs, with potential applications in epidemiological studies and post-treatment follow-up. Further validation with larger sample sizes is needed to confirm its diagnostic accuracy in natural infections.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110962"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does gradient isolation preserve equine neutrophil viability and oxidative function for up to six hours?","authors":"Laís Sodré Santana Alves ,&nbsp;Amanda Lopes Hasuda ,&nbsp;Lucienne Garcia Pretto Giordano ,&nbsp;Isabela Frederico ,&nbsp;Isabelle Hadid dos Santos ,&nbsp;Priscilla Fajardo Valente Pereira ,&nbsp;Júlio Augusto Naylor Lisbôa ,&nbsp;Karina Keller Marques da Costa Flaiban","doi":"10.1016/j.vetimm.2025.110961","DOIUrl":"10.1016/j.vetimm.2025.110961","url":null,"abstract":"<div><div>This study investigated isolation of equine neutrophils and the assessment of their antioxidant function using the nitroblue tetrazolium (NBT) reduction test, an indicator of reactive oxygen species (ROS) activation. The objective was to test the viability of neutrophils isolated from equine whole blood and their ability to undergo respiratory burst, comparing the results with whole blood. The hypothesis tested was that isolation of equine neutrophils allows for effective functional evaluation, even after isolation process, with no significant differences between whole blood and isolated cells. The study showed that neutrophils maintained their ability to reduce NBT dye over a 6-hour period, with no significant differences between time points analyzed (0 h, 3 h, and 6 h). Isolation was performed using Histopaque®, and total neutrophil concentration and oxidative function were evaluated at three different time points. Cell counts and viability assessments were conducted using both automated and manual techniques, confirming the prolonged viability of neutrophils and the accuracy of antioxidant function testing.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110961"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Evaluation of serum serotonin as a biomarker of intestinal inflammation in calves” [Vet. Immunol. Immunopathol. 284 (2025) 110947]","authors":"Murat Uzti̇mür,&nbsp;Aysu Ece Şengül,&nbsp;Cennet Nur Ünal","doi":"10.1016/j.vetimm.2025.110959","DOIUrl":"10.1016/j.vetimm.2025.110959","url":null,"abstract":"","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"285 ","pages":"Article 110959"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}