Veterinary journal最新文献

{"title":"The hidden cost of caring: Psychological burden among caregivers of dogs with cognitive dysfunction, cancer, and age-related decline","authors":"Darja Pavlin ,&nbsp;Valentina Hlebec ,&nbsp;May Doušak ,&nbsp;Maja Zakošek Pipan","doi":"10.1016/j.tvjl.2025.106510","DOIUrl":"10.1016/j.tvjl.2025.106510","url":null,"abstract":"<div><div>Canine cognitive dysfunction (CCD) is a neurodegenerative disorder like Alzheimer's disease in humans, but its impact on caregivers is poorly understood. This study examines the multidimensional burden of caregivers of ageing dogs, comparing caregivers of healthy ageing dogs (HAD), dogs with chronic oncological disease (COD) and dogs with CCD. In a cross-sectional survey of 516 Slovenian dog owners, seven areas of stress were investigated: time burden, work- life interference, emotional burden, social stigmatization, financial burden, perceived adequacy of care and veterinary support. Time burden was increased by unpredictable CCD symptoms (e.g. nocturnal restlessness, disorientation), especially in households with children or multiple pets (p = 0.005). Emotional distress was significantly increased in CCD caregivers due to feelings of stigmatization (p = 0.005), while COD caregivers reported no significant increase compared to HAD. Caregivers of dogs with CCD reported significantly greater stigmatization (p &lt; 0.001) and financial burden (p &lt; 0.001). While all groups reported similar perceived inadequacies in care, CCD caregivers expressed significantly lower confidence in performing positive care tasks (p = 0.041). Relationships with veterinarians did not differ between groups, although regression analysis identified age, education and number of pets as predictors of the dynamics between caregivers and veterinarians. This study shows that caring for dogs with CCD is significantly more stressful than caring for healthy older dogs and, in several areas, caring for dogs with chronic oncologic diseases. The results emphasize that caring for dogs with CCD is a complex, underestimated burden that is comparable to caring for dementia patients in humans and requires targeted interventions.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106510"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145640151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultural adaptation and psychometric properties of the canine owner-reported quality of life questionnaire (CORQ) for assessing quality of life in dogs with cancer","authors":"Manuel Fuertes-Recuero DVM, PhD ,&nbsp;Paula Rodriguez-González DVM ,&nbsp;María Suárez-Redondo DVM, PhD ,&nbsp;Miriam Portero DVM, PhD ,&nbsp;Andrés Yzuel DVM ,&nbsp;Silvia Penelo DVM, PhD ,&nbsp;Carmen Pérez DVM, PhD ,&nbsp;Elena Martínez de Merlo DVM, PhD ,&nbsp;Michelle A. Giuffrida VMD, MSCE ,&nbsp;Gustavo Ortiz-Díez","doi":"10.1016/j.tvjl.2025.106503","DOIUrl":"10.1016/j.tvjl.2025.106503","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to translate and culturally adapt the Canine OwnerReported Quality of Life (CORQ) questionnaire, developed by Giuffrida et al. (2018), to evaluate the quality of life (QoL) in dogs with cancer, following World Health Organization (WHO) guidelines. The QoL is becoming increasingly important in veterinary medicine, especially in the context of companion animals diagnosed with cancer. Many existing studies use non-validated scales, resulting in a lack of reliable instruments in Spanish-speaking populations.</div></div><div><h3>Methods</h3><div>Construct validity and internal consistency were evaluated using factor analyses and Cronbach’s α, and convergent validity was examined against established quality-of-life measures.</div></div><div><h3>Results</h3><div>The findings indicate that the Spanish version of the CORQ exhibits robust psychometric properties, characterized by high internal consistency (Cronbach’s alpha = 0.841 for vitality and 0.946 for wellness) and significant correlations with established QoL measures.</div></div><div><h3>Conclusions and clinical relevance</h3><div>This successful adaptation and validation provides Spanish-speaking veterinarians with a reliable tool to assess and manage quality of life in dogs diagnosed with cancer.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106503"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145640185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of three anaesthetic protocols (dexmedetomidine/ketamine, dexmedetomidine/midazolam, midazolam/ketamine) in dwarf rabbits undergoing elective orchiectomy","authors":"Alessio Angorini ,&nbsp;Carlotta Cacchiani ,&nbsp;Luca Pennasilico ,&nbsp;Margherita Galosi ,&nbsp;Adolfo Maria Tambella ,&nbsp;Romina Marcoccia ,&nbsp;Fabrizio Dini ,&nbsp;Caterina Di Bella","doi":"10.1016/j.tvjl.2025.106525","DOIUrl":"10.1016/j.tvjl.2025.106525","url":null,"abstract":"<div><div>This prospective clinical study aimed to compare three different anaesthetic protocols in dwarf rabbits and analyse the effects of these pharmacological combinations from premedication to complete recovery. Twenty-one pet rabbits were randomly allocated in study groups – MKM (7 rabbits): midazolam (0.2 mg/kg), ketamine (20 mg/kg), and methadone (0.3 mg/kg) were administered subcutaneously (SC); DKM (7 rabbits): received dexmedetomidine (25 µg/kg), ketamine (20 mg/kg), and methadone (0.3 mg/kg) SC; MDM (7 rabbits): received midazolam (0.2 mg/kg), dexmedetomidine (25 µg/kg), and methadone (0.3 mg/kg) SC. Ten minutes before premedication and every 5 min for 20 min (T5, T10, T15, and T20, respectively), heart rate, respiratory rate, and sedation score (SS) were recorded. Cases of ataxia/incoordination were also reported. After induction of general anaesthesia, main cardiovascular and respiratory parameters were monitored during skin incision, testicular traction, and suture placement. At the end of the procedure, extubation time, recovery of sternal recumbency and quadrupedal stance, and quality of recovery (QR) were recorded. The DKM group showed higher SS at T10 than the other two groups; however, 4 out of 7 subjects manifested ataxia and incoordination, compared to the MDM, in which no episodes occurred. Time to extubation in the MKM was longer (6.8 ± 3.5 min) compared to the MDM (2.6 ± 2.07 min); moreover, QR was worse in the DKM and MKM groups compared to MDM. The combination of dexmedetomidine/ketamine provides better sedation; however, it frequently induces ataxia/incoordination. The further addition of midazolam could promote muscle relaxation and tranquillisation, reducing handling stress in pet rabbits.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106525"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145679086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal trends and risk factors of small-ruminant brucellosis in China: A systematic review and meta-analysis","authors":"Wei Wei ,&nbsp;Di Jiao ,&nbsp;Rui Shi ,&nbsp;Rigai Sa ,&nbsp;Zhengyi Li ,&nbsp;Lu An ,&nbsp;Xiaoqian Zhang ,&nbsp;Jingyi Wen ,&nbsp;Rui Wang","doi":"10.1016/j.tvjl.2026.106547","DOIUrl":"10.1016/j.tvjl.2026.106547","url":null,"abstract":"<div><div>Brucellosis remains a neglected zoonosis that threatens livestock production and human health in China. Small ruminants are key reservoirs, yet brucellosis in sheep and goats is still insufficiently characterized in terms of seroprevalence, risk factors, and spatial–temporal dynamics. We conducted a nationwide systematic review and meta-analysis (PRISMA) of studies published up to 1 August 2025. We pooled eligible cross-sectional data using a random-effects model, explored determinants by subgroup analysis and meta-regression, and applied ARIMA models to forecast trends. We included 306 studies from 27 provinces. The overall pooled seroprevalence was 3.0 % (95 % CI 2.3–3.8), with strong geographic heterogeneity. The highest burden occurred in North China, especially Inner Mongolia. Risk was higher in spring and in BSk (cold semi-arid) climates. Seroprevalence was slightly higher in goats, young animals (&lt;1 year), and stall-feeding systems, indicating the need to strengthen housing hygiene and farm biosecurity. Diagnostic methods explained substantial heterogeneity: RBPT tended to yield higher estimates, whereas ELISA/cELISA offer better diagnostic performance. Seroprevalence rose around 2016–2017 and then declined only modestly. Forecasts suggested a continued upward drift (0.76 % in 2026–0.84 % in 2027), implying sustained One Health risks. These findings support region-specific vaccination with priority for goats, expanded standardized ELISA/cELISA use, and integrated human–livestock surveillance with harmonized reporting and targeted protection for high-risk workers in hotspot regions.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106547"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and clinical significance of Mycoplasma bovis infection in calf feedlots in Spain","authors":"Christian de la Fe ,&nbsp;Xóchitl Hernández ,&nbsp;Juan Alcázar ,&nbsp;Antonio Sánchez ,&nbsp;David del Olmo ,&nbsp;Antonio Fernández ,&nbsp;Héctor Ramírez ,&nbsp;Ginés Luján ,&nbsp;Joaquín Amores-Iniesta ,&nbsp;Juan Carlos Corrales","doi":"10.1016/j.tvjl.2025.106517","DOIUrl":"10.1016/j.tvjl.2025.106517","url":null,"abstract":"<div><div>The presence, dynamics and persistence of <em>Mycoplasma (M.) bovis</em> in 12 batches of grazing calves during the first month of stay at the feedlots was analyzed. Nasopharyngeal swabs were weekly collected between days 0 and 28 to detect the presence of <em>M. bovis</em> by culture and PCR. The animals were daily monitored for the presence of respiratory disease. The results showed the presence of different epidemiological situations at arrival, detecting the presence of uninfected or with a low prevalence groups or batches with a variable prevalence (13.3–86.7 %). Subsequently, at least one calf scored positive in all the batches 14 days after arrival and almost all calves (n = 166) resulted infected after 28 days. Calves infected with <em>M. bovis</em> tested positive in 3 of the 5 samples taken (mean 3.06). The presence of respiratory signs was statistically significant in infected <em>vs</em>. uninfected ones, with symptoms appearing earlier in batches where the initial prevalence of <em>M. bovis</em> was higher. The molecular characterization of the isolates confirmed the presence of more than one biotype circulating in the same batch or even calf, which could be associated to the presence of more severe clinical findings. Most of the isolates were classified as biotype st-3 but three biotypes were detected, resulting all of them multiresistant to macrolides, tetracyclines and lincomycin with a variable sensitivity to quinolones. Overall, the frequency of <em>M. bovis</em> infection highlights its significant epidemiological role in bovine respiratory diseases in calf fattening.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106517"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145640113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and field evaluation of a competitive ELISA to detect specific antibodies against Anaplasma marginale","authors":"Julio Bellezze ,&nbsp;Beatriz Susana Valentini ,&nbsp;Anabela Silvina Bosio,&nbsp;Carolina Soledad Thompson,&nbsp;María Evangelina Primo","doi":"10.1016/j.tvjl.2025.106528","DOIUrl":"10.1016/j.tvjl.2025.106528","url":null,"abstract":"<div><div>A species-specific competitive ELISA for <em>Anaplasma marginale</em> antibodies detection (cELISA-sAm) was developed by using both a monoclonal antibody specific to an epitope of MSP5 of <em>A. marginale</em> (Am6-mAb) and adding a pre-incubation phase with the truncated MSP5 of <em>A. centrale</em> (tMSP5c). For the determination of the cutoff value, diagnostic sensitivity (DSe) and specificity (DSp) of cELISA-sAm, 382 samples from cattle not infected with <em>Anaplasma</em> spp., 237 samples from cattle naturally infected with <em>A. marginale,</em> and 102 samples from cattle vaccinated with <em>A. centrale</em> were analyzed. The infection status was established using nested PCR (nPCR) or nPCR-restriction fragment length polymorphism assay (nPCR-RFLP) as reference technique. In a field evaluation, cELISA-sAm and nPCR-RFLP were used on <em>Anaplasma</em> spp. positive samples by double-antigen sandwich ELISA (dasELISA) from 2 herds to confirm the <em>A. marginale</em> infection. The cELISA-sAm optimal cutoff value was ≥ 18 %I, with a DSe of 89.9 % and a DSp of 99.0 %. In a field evaluation, concordance between cELISA-sAm and nPCR-RFLP was 93.0 % with κ = 0.81 (95 % CI = 0.56–1.00) for <em>A. marginale</em>-infected cattle detection. In countries in which prevention is based on vaccination with <em>A. centrale</em>, the replacement of current ELISAs by this cELISA-sAm would decrease the false positive results and avoid the need of species confirmation by molecular techniques when disease control programs are performed to monitor the prevalence of <em>A. marginale</em>.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106528"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145702066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of human Wharton’s Jelly-derived mesenchymal stem cells (hWJ-MSCs) for treating clinical and subclinical mastitis in dairy cows","authors":"Durmus Hatipoglu ,&nbsp;Mehmet Burak Ates ,&nbsp;Ayla Eker Sariboyaci ,&nbsp;Onur Uysal ,&nbsp;Merve Nur Soykan ,&nbsp;Burcugul Altug ,&nbsp;Bahar Demir Cevizlidere ,&nbsp;Fulya Buge Ergen ,&nbsp;Aynaz Ghorbani ,&nbsp;Fatih Aladag","doi":"10.1016/j.tvjl.2025.106534","DOIUrl":"10.1016/j.tvjl.2025.106534","url":null,"abstract":"<div><div>This pioneering study explored the therapeutic potential of human Wharton’s jelly derived mesenchymal stem cells (hWJ-MSCs) as a novel regenerative treatment for subclinical (SCM) and clinical mastitis (CM) in dairy cows, a condition that poses significant economic and health challenges. hWJ-MSCs were isolated and characterized, and then administered intramammarily in two doses, seven days apart, to twelve cows that were divided into control, SCM, and CM groups. Comprehensive evaluations, including somatic cell count (SCC), proinflammatory cytokine profiles, milk yield, and bacteriological analyses, were conducted before treatment and on days 14 and 21 after treatment. The results revealed a remarkable reduction in SCC by 29.62 % in the SCM group and 70.46 % in the CM group, accompanied by a pronounced suppression of proinflammatory cytokines such as TNF-α and IL-1β. hWJ-MSCs therapy also led to substantial improvements in milk yield, with increases of 39.38 % and 14.32 % in the SCM and CM groups, respectively. Moreover, bacteriological analyses revealed the complete eradication of pathogenic agents, including <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, in nearly all treated patients. These findings underscore the potential of hWJ-MSCs to redefine mastitis management by offering a safe, effective, and antibiotic-free alternative, addressing both agricultural and global health concerns related to antimicrobial resistance. The significant therapeutic effects observed in this study suggest that hWJ-MSCs could be used to treat mastitis, paving the way for advanced stem cell-based interventions in veterinary medicine. Further large-scale studies are warranted to validate these promising results and establish hWJ-MSCs as a cornerstone of sustainable livestock health management.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106534"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145744813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of classical music and supervisory authority on anxiety and surgical performance in veterinary students during first ovariohysterectomy","authors":"Atakan Cortu ,&nbsp;Muhammed Albayrak ,&nbsp;Halil Ozan Demirtas ,&nbsp;Latif Emrah Yanmaz","doi":"10.1016/j.tvjl.2025.106518","DOIUrl":"10.1016/j.tvjl.2025.106518","url":null,"abstract":"<div><div>Anxiety during the first live-animal surgery represents a major challenge in veterinary training, potentially affecting both performance and learning outcomes. Classical music has been suggested as a non-invasive strategy to moderate stress, while the degree of supervisory authority may further modulate students’ emotional and behavioural responses. This study evaluated the effects of classical music exposure and supervisory authority on perioperative anxiety and surgical performance in final-year veterinary students performing their first feline ovariohysterectomy. Forty students were randomly allocated into four groups (n = 10 each): assistant professor supervision without music (AP), assistant professor with music (AP-M), research assistant without music (RA), and research assistant with music (RA-M). Music consisted of Mozart’s Sonata for Two Pianos in D Major, K.448, delivered via headphones for 30 min preoperatively. Anxiety was measured using the State-Trait Anxiety Inventory before and after intervention and following surgery. Operative duration was recorded from skin incision to closure. The AP-M was associated with increased state anxiety, whereas no significant changes occurred in other groups. Operative times were longest in AP-M and RA, and shortest in RA-M. Trait anxiety correlated positively with operative duration, while state anxiety was not directly linked to performance. In conclusion, music may heighten anxiety under high-authority supervision but improve efficiency in less authoritative contexts. Educational strategies should prioritise supportive supervision and consider tailoring auditory interventions to optimise student wellbeing and performance.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106518"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of dry-period heat stress on inflammatory, oxidative and metabolic alterations, and acquired immunity in dairy cows and offspring","authors":"Mehmet Akköse ,&nbsp;Halit Kanca ,&nbsp;Tuğrul Hoşbul ,&nbsp;Sema Ören ,&nbsp;Murat Onur Yazlık ,&nbsp;Ufuk Kaya ,&nbsp;Mert Pekcan ,&nbsp;Hatice Esra Çolakoğlu ,&nbsp;Yunus Furkan Altınbaş ,&nbsp;Hasan Karakuş ,&nbsp;Mehmet Rıfat Vural","doi":"10.1016/j.tvjl.2026.106550","DOIUrl":"10.1016/j.tvjl.2026.106550","url":null,"abstract":"<div><div>This study aimed to investigate the effects of dry-period heat stress on metabolic and oxidative stress parameters, cytokine release, and T lymphocyte percentages in dairy cows and their offspring. Thirty-two Holstein cows were randomly assigned to heat stress (HS) and cooling (CL) groups. Blood samples were collected to harvest serum and isolate peripheral blood leukocytes, from cows at 21 – 28 days before the expected calving date and immediately after calving, and from their calves postnatally (before colostrum consumption and 24 ± 1 h after birth). Helper (Th1, Th2), regulatory T (Treg), and gamma/delta T (γδT) lymphocytes were enumerated by flow cytometry. Interferon gamma (IFN-γ), tumor necrosis factor- alpha (TNF-α), Interleukin-17A (IL-17A), IL-10, IL-6, IL-1β, retinol, alpha-tocopherol, lactoferrin, and immunoglobulin G (IgG) levels were measured using bovine-specific commercial enzyme-linked immunosorbent assay (ELISA) test kits. Nitric oxide, paraoxonase, total oxidant capacity, total antioxidant capacity, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase, malondialdehyde (MDA), albumin, total protein, cholesterol, bilirubin, and creatinine levels were measured using an auto-analyzer. Compared to the CL group, in the HS cows, serum retinoic acid, alpha-tocopherol (p &lt; 0.05), ALP, catalase, GSH-Px and IL-1β levels (p &lt; 0.01) were significantly lower, whereas IL-6, IFN-γ and IL-10 levels were higher (p &lt; 0.01). The HS cows presented with higher Th1 lymphocyte percentages (p &lt; 0.01), and lower γδT and Treg lymphocyte percentages (p &lt; 0.01). Treg lymphocyte percentages showed no difference between the groups, but varied by day. Specifically, in both the HS and CL dams, Treg lymphocyte percentages were significantly lower at the calving time than on day 21 before the expected calving date (p = 0.021). Colostral IgG concentrations were lower (p &lt; 0.05) in the HS group. In the offspring, there were no significant differences between the HS and CL groups for the T lymphocyte percentages before colostrum consumption and the serum IgG concentrations at 24 ± 1 h after birth. In conclusion, the study findings suggest that providing heat stress abatement to dry cows may modulate both Th1, Treg, and γδT lymphocyte functions, and the release of pro-inflammatory and anti-inflammatory cytokines. However, intrauterine heat stress did not affect lymphocyte percentages in offspring before colostrum consumption.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106550"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145935337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal microRNA profiling reveals candidate biomarkers and regulatory signatures associated with paratuberculosis disease status in goats","authors":"Eun-Yeong Bok ,&nbsp;Tae Yoon Kim ,&nbsp;Sang-Young Seo ,&nbsp;Han-Gyu Lee ,&nbsp;Young-Hun Jung ,&nbsp;Seong Hwan Bae ,&nbsp;Nam Su Oh ,&nbsp;Tae Jin Cho ,&nbsp;Hyeon Woo Park ,&nbsp;Eui Cheol Shin ,&nbsp;Jae Kyeom Kim","doi":"10.1016/j.tvjl.2026.106553","DOIUrl":"10.1016/j.tvjl.2026.106553","url":null,"abstract":"<div><div>Paratuberculosis (Johne’s disease), a chronic granulomatous enteritis caused by <em>Mycobacterium avium</em> subspecies <em>paratuberculosis</em> (MAP), is difficult to diagnose in the subclinical stage(s) because of intermittent shedding and nonspecific immune responses. Given their stability and potential as biomarkers, exosomal microRNAs (miRNAs) were investigated in this study to identify serum-derived candidates associated with Paratuberculosis-associated disease stages in goats. Exosomes were isolated from the serum of three groups: Control (ELISA- and PCR-negative), ENPP (ELISA-negative but PCR-positive), and EPPP (ELISA- and PCR-positive). After exosome characterization, small RNA sequencing and bioinformatic analyses were performed to identify differentially expressed miRNAs (DEmiRs) and their predicted regulatory targets. Functional enrichment, upstream regulator, and network analyses were then applied to interpret the biological relevance of the identified DEmiRs. Among the candidates, miR-122 and miR-21–5p were consistently upregulated in infected animals and were associated with immune modulation, epithelial integrity, and inflammation. Finally, quantitative PCR validation confirmed a significant elevation of these miRNAs in the EPPP group, supporting their potential as late-stage biomarkers. Our findings demonstrate that exosomal miRNA profiling can reflect MAP infection status and highlight their potential utility as non-invasive biomarkers for Paratuberculosis monitoring in veterinary settings.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106553"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145953101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}