Veterinary Medicine International最新文献

{"title":"Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods.","authors":"Enas Safi,&nbsp;Moawiya Haddad,&nbsp;Maen Hasan,&nbsp;Sati Y Al-Dalain,&nbsp;Charalampos Proestos,&nbsp;Shahida A Siddiqui","doi":"10.1155/2023/8334152","DOIUrl":"10.1155/2023/8334152","url":null,"abstract":"<p><p>A total of 60 isolates of lactic acid bacteria (LAB) were isolated from Jordanian camel colostrum using biochemical and molecular methods. Two dominant species were identified, and they were <i>Lactobacillus salivarius</i> and <i>Enterococcus faecium</i>. The entire 60 isolated LAB were tested for their acidity and bile tolerance, antimicrobial activity, and antibiotic sensitivity to test their potential probiotic activity. All 60 isolates were tolerant to different pH concentrations (2, 3, 4, 5, 6, 7, 8, 9, and 10) with different survival rates (%). The entire isolates were also tolerant to different bile salt concentrations (0.2, 0.4, 0.6, 0.8, 1, 2, and 3) with different bile resistance (%). All isolates have a different range of antimicrobial activity against <i>Staphylococcus aureus</i>, <i>E. coli</i>, and <i>Salmonella typhimurium</i>. The 60 isolates were almost sensitive to ampicillin, amoxicillin, and clarithromycin when different concentrations were used except some isolates of intermediate resistance. Only 6% of the isolates were resistant to clarithromycin at a concentration of 15 <i>µ</i>g per disc.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"8334152"},"PeriodicalIF":3.1,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41238909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Determination of the Seminiferous Epithelium Stages and Spermatid Development in the Testis of Aceh Bull (<i>Bos indicus</i>).","authors":"Sri Wahyuni,&nbsp;Tongku Nizwan Siregar,&nbsp;Gholib Gholib,&nbsp;Arianto Saputra,&nbsp;Hafizuddin Hafizuddin,&nbsp;Hamny Sofyan,&nbsp;Muhammad Jalaluddin,&nbsp;Mulyadi Adam,&nbsp;Muslim Akmal","doi":"10.1155/2023/8848185","DOIUrl":"https://doi.org/10.1155/2023/8848185","url":null,"abstract":"<p><p>This study was conducted to describe the stages of seminiferous epithelium (SE), determine the relative frequency of the stages, and identify the steps of spermatid development during spermatogenesis in the testicular tissue of Aceh bull. Seven pairs of the testicular organs of Aceh bull <i>(Bos indicus)</i> were used and then processed in a histological manner for staining using haematoxylin and eosin (H&E) and periodic acid-Schiff-haematoxylin (PAS-H). The stages of seminiferous tubules were examined using a tubular morphology method while spermatid development was observed based on the acrosome formation during spermatid development. Eight stages (stages I to VIII) of SE were found in the testicular seminiferous tubules of Aceh bull. Furthermore, the percentage of the relative frequency of each stage was 25.48, 15.38, 12.92, 4.74, 14.97, 10.69, 10.74, and 5.08%, respectively, with the relative frequency of premeiotic, meiotic, and postmeiotic phases being 53.78, 4.74, and 41.48%, respectively. Spermatid development from round to elongated spermatids occurred in 14 steps. Steps 1 to 7 were observed in stage I, steps 8 and 9 in stage II, steps 10 and 11 in stage III, step 12 in stage IV, step 13 in stages V and VI, and step 14 in stages VII and VIII. These findings can be used as a basis for further studies, particularly in evaluating the abnormality of the cellular composition of the seminiferous tubule in each stage of spermatogenesis and also in determining daily sperm production in Aceh bull.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"8848185"},"PeriodicalIF":3.1,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41135163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Corticosterone Concentrations in Dermal Secretions and Urine in Free-Ranging Marine Toads (<i>Rhinella marina</i>) in Human Care.","authors":"Emma Clarke,&nbsp;Kimberly Ange-van Heugten,&nbsp;Troy N Tollefson,&nbsp;Frank N Ridgley,&nbsp;Dustin Smith,&nbsp;Janine L Brown,&nbsp;Heather Scott,&nbsp;Larry J Minter","doi":"10.1155/2023/1467549","DOIUrl":"https://doi.org/10.1155/2023/1467549","url":null,"abstract":"<p><p>Corticosterone concentrations have been measured in amphibians by collecting blood or urine samples. However, blood sampling is invasive, and urine can be difficult to collect. A novel method of swabbing the skin of an amphibian has been utilized in numerous species but has not been verified in marine toads (<i>Rhinella marina</i>). This pilot study tested dermal swabs as a noninvasive method for collecting and measuring dermal corticosterone secretions. Swabs were used to collect dermal secretion samples from sixty-six free-ranging marine toads collected on Zoo Miami grounds. The subsequent day the toads were shipped to the North Carolina Zoo where dermal samples were collected again. Additional dermal and urine samples were collected on days 9, 15, 32, and 62 under human care to measure corticosterone concentrations. There was no significant correlation (<i>P</i> ≥ 0.05) noted between corticosterone concentrations reported in dermal swabs and those in urine samples at all four of the euthanasia time points or between the corticosterone concentrations reported in either urine or dermal swabs and the weight of the toads. Dermal swab concentrations (ng/mL) were significantly higher (<i>P</i> ≤ 0.05) on the day of capture (0.64 ± 0.03) and the day of arrival (0.67 ± 0.03) than on day 15 (0.47 ± 0.03). The urine corticosterone concentrations decreased while the toads were in human care with a significant decrease (<i>P</i> ≤ 0.05) between days 9 (0.45 ± 0.07) and 32 (0.21 ± 0.06). This study demonstrated that dermal swabs can be used to collect marine toad corticosterone concentration samples.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"1467549"},"PeriodicalIF":3.1,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic Screening of Dairy Cows with Normal Uterine Involution or Developing Postpartum Uterine Disease Using B-Mode, Color, and Spectral Doppler.","authors":"Mariana Henao-Gonzalez,&nbsp;María S Ferrer,&nbsp;Claudia Jiménez-Escobar,&nbsp;Luis G Palacio-Baena,&nbsp;Juan G Maldonado-Estrada","doi":"10.1155/2023/2597332","DOIUrl":"https://doi.org/10.1155/2023/2597332","url":null,"abstract":"<p><p>Uterine involution, ovarian activity, and incidence of postpartum uterine disease (PUD) were assessed in forty-eight dairy cows from calving until the 10^th postpartum week. Postpartum follow-up included evaluation of uterine involution and ovarian structures by B-mode, Doppler color, and Doppler spectral ultrasound of the right uterine artery in cows with no calving or postpartum uterine problems (healthy cows). Data from cows that developed PUD (PUD cows) were compared with healthy cows matched by herd and days in milk (DIM). Data were analyzed by descriptive statistics, simple regression, one-way ANOVA, or repeated ANOVA measures, and in data analysis of healthy cows, uterine horn diameter assessed by B-mode ultrasound ranged from 22.9 ± 2.4 to 19.4 ± 1.4 mm and 19.9 ± 2.2 to 20.5 ± 2.3 mm from the fourth to the seventh postpartum week in the left and right uterine horns, respectively (<i>P</i> > 0.05). During the study, 15 and 7 cows had <i>corpus luteum</i> in the left and right ovaries, respectively. The mean time for the first postpartum CL was 30.1 ± 3.2 DIM (min 8, max 67 DIM). In data analysis of PUD cows, uterine blood flow assessed by color Doppler ranged from 7.4 ± 4.0 to 43.75 ± 10.3% in cows that developed PUD compared to 16.7 ± 11.0% in healthy cows (<i>P</i> > 0.05). No statistically significant changes were found in resistance index, pulsatility index, time-averaged maximum velocity, time-averaged mean velocity, or diastole/systole ratio (D/S) in cows that developed PUD compared to healthy cows (<i>P</i> > 0.05). Finally, no correlation was found between Doppler spectral parameters and uterine involution (<i>P</i> > 0.05). Our data suggest that cows receiving transition diets and exhibiting normal calving undergo a rapid macroscopic uterine involution and ovarian follicular dynamics resumption. Complete ultrasound evaluation provides valuable data for assessing uterine involution in postpartum dairy cows.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"2597332"},"PeriodicalIF":3.1,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41178570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper Super-Dosing Improves Performance of Heat-Stressed Broiler Chickens through Modulation of Expression of Proinflammatory Cytokine Genes.","authors":"Sudipto Haldar,&nbsp;Amrita Kumar Dhara,&nbsp;Sayantani Sihi Arora,&nbsp;Arpana Verma Mukherjee,&nbsp;Arup Nayak","doi":"10.1155/2023/3559234","DOIUrl":"https://doi.org/10.1155/2023/3559234","url":null,"abstract":"<p><p>Continuous exposure to high ambient temperatures brings about a number of oxidative damages in chickens. Copper (Cu), an active component of a number of antioxidative defence components, should arrest these changes to take place although that may not be possible under the standard dosing regimen followed by the industry. To ascertain the optimum dose response that may be beneficial in sustaining the performance of chickens under heat stress (HS), broiler chickens (<i>n</i> = 400) were exposed to high ambient temperature (between 27.2°C and 35.3°C) during 1-35 d. Copper (Cu) as Cu proteinate (Cu-P) at concentrations of 37.5, 75, 112.5, and 150 mg/kg was supplemented to the diet. The negative control (NC) diet did not contain any supplemental Cu. Increasing dietary Cu improved (<i>P</i> < 0.001) body weight, feed intake, and conversion ratio. Serum concentrations of total cholesterol at 21 d (<i>P</i> = 0.009), HDL cholesterol at 35 d (<i>P</i> = 0.008), LDL cholesterol at 21 d (<i>P</i> = 0.015), and triacylglycerol at both 21 d (<i>P</i> = 0.033) and 35 d (<i>P</i> = 0.001) decreased as Cu in the diet increased. As Cu in the diet increased, hemoglobin increased (<i>P</i> = 0.003) at 21 d, and the heterophil to lymphocyte ratio decreased both at 21 d (<i>P</i> = 0.047) and 35 d (<i>P</i> = 0.001). Superoxide dismutase and glutathione peroxidase activities increased when dietary Cu increased to 150 mg/kg (<i>P</i> < 0.01). Liver Cu at 35 d increased linearly with the dose of Cu in the diet (<i>P</i> = 0.0001). Selected bacteria were enumerated in the digesta to ascertain if Cu super-dosing affected their population in any way in the absence of any enteric challenge. <i>Escherichia coli</i> and total <i>Salmonella</i> numbers decreased (<i>P</i> = 0.0001), and total <i>Lactobacillus</i> increased (<i>P</i> = 0.0001) proportionately with dietary Cu. Interleukin-6 and tumour necrosis factor-<i>α</i> gene expression increased linearly (<i>P</i> = 0.0001) as Cu in the diet increased though the response plateaued at 112.5 mg/kg. It was concluded from the present experiment that during conditions of impending HS, dietary supplementation of 112.5 to 150 mg Cu/kg diet as Cu-P may be a novel strategy to alleviate the negative effects of HS without involving any apparent risk of Cu toxicity.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"3559234"},"PeriodicalIF":3.1,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41178569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extender Supplementation with Glutathione (GSH) and Taurine Improves <i>In Vitro</i> Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours.","authors":"Mohamed F F Bayomy,&nbsp;Sobhy E Hassab El-Nabi,&nbsp;Tahany A El Kassas,&nbsp;Zeinab I Attia,&nbsp;Ayman M Saeed,&nbsp;Heba S A Taha,&nbsp;Mahmoud Alagawany,&nbsp;Livio Galosi,&nbsp;Lucia Biagini,&nbsp;Seham El-Kassas","doi":"10.1155/2023/8339591","DOIUrl":"https://doi.org/10.1155/2023/8339591","url":null,"abstract":"<p><p>This study assessed the influence of supplementing the rabbit semen extender with various concentrations of glutathione (GSH) and taurine at 24, 48, and 72 h postchilling at 5°C. Semen samples were collected from 20 New Zealand bucks, and ejaculates with standard color, motility (>85%), about 0.5 mL volume, and ∼400 × 10⁶/mL concentration were used and diluted with extenders supplemented with 0.5, 1, and 2 mM of GSH and 1, 5, and 10 mM of taurine and chilled at 5°C. Nonsupplemented samples were used as a control. Sperm's progressive motility, acrosome reaction, and extracellular oxidative stress biomarkers such as MDA contents and GPx, SOD, and CAT concentrations and intracellular transcriptomic levels of <i>SOD</i> and <i>CAT</i> genes were assessed. GSH and taurine supplementation improved the sperm's kinetics by reducing cooling-associated stress, which was ascertained by lowering MDA concentration and increasing SOD, CAT, and GPx concentrations (<i>P</i> < 0.05). Increasing the levels of antioxidant enzymes in the extender was due to the increasing mRNA copies of the <i>SOD</i> and <i>CAT</i> genes (<i>P</i> < 0.05). Furthermore, GSH and taurine maintained the fructose levels in the extender and lowered the GPT levels, which implies sperm membrane stability is maintained through GSH and taurine supplementation. GSH and taurine supplementation to the extender had protective influences on the <i>in vitro</i> rabbit semen quality during chilled storage for up to 72 h, which were remarkable with increasing supplementation dose and cooling time at 5°C.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"8339591"},"PeriodicalIF":3.1,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41152595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Mink Immunity and Resistance to Pseudomonas aeruginosa","authors":"Jiangsong Bai, Xiao Wang, Zihui Zhang, Pengjing Lian, Jian Qiao","doi":"10.1155/2023/6158844","DOIUrl":"https://doi.org/10.1155/2023/6158844","url":null,"abstract":"Pseudomonas aeruginosa is one of the most host-susceptible pathogenic bacteria to cause acute and chronic infections in humans and animals. Notably, its infection can especially cause fatal pathogenic infectious pneumonia in minks. Many previous mink studies have investigated the pathology, pathogenesis, serology, antimicrobial resistance, virulence gene, vaccination, and related diseases of P. aeruginosa. However, the relationship of P. aeruginosa infections with mink immunity and resistance is relatively less studied that needs more elaborations. Therefore, we here provide a comprehensive review about mink immunity and resistance to P. aeruginosa infections and the pathogenesis of mink hemorrhagic pneumonia in four major aspects. They include characterization, infection, immunity, and resistance of P. aeruginosa, and their implication and perspective, which aim to contribute the useful and valuable information to further related research and clinical treatment of P. aeruginosa and to avoid the potential fatal hemorrhagic pneumonia spreading.","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"38 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81732117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection and Multidrug Resistance of Shigella spp. Isolated from Wild Waterfowl and Migratory Birds in Bangladesh","authors":"Jarna Karmoker, M. Saiful Islam, Md. Liton Rana, Md. Ashek Ullah, Fahim Haque Neloy, Nahian Muniath Oishy, Pritom Kumar Pramanik, M. P. Siddique, S. Saha, M. T. Tanvir Rahman","doi":"10.1155/2023/5374216","DOIUrl":"https://doi.org/10.1155/2023/5374216","url":null,"abstract":"Birds, especially wild waterfowl and migratory birds have the potential to carry antibiotic-resistant bacteria, but their role in the dissemination of these resistant pathogens is still neglected in Bangladesh. To the best of our knowledge, this study was carried out for the first time in Bangladesh to isolate and determine the occurrence of multidrug-resistant (MDR) Shigella spp. from fecal materials of wild waterfowl and migratory birds. A total of 80 fecal materials from wild waterfowl (n = 50) and migratory birds (n = 30) were screened to detect MDR Shigella isolates. Shigella spp. were isolated and identified by culturing, staining, and biochemical tests followed by polymerase chain reaction (PCR). A disk diffusion assay was employed to investigate antibiotic phenotypes, while the resistance genes were detected by PCR. Among the 80 samples, 15 (18.75%) were found positive for Shigella spp. by PCR, among which the occurrence rate of Shigella spp. was higher in migratory birds (20%, 6/30) than in wild waterfowl (18%, 9/50). By the disk diffusion test, 86.67% (13/15) of Shigella spp. isolates were found to be MDR in nature, including 93.33% of isolates resistant to imipenem. Moreover, frequent and moderate resistance was also observed against tetracycline (86.67%), azithromycin (80%), ampicillin (66.67%), ciprofloxacin and cotrimoxazole (40%), meropenem (26.67%), and streptomycin (13.33%). The bivariate analysis revealed a positive correlation between the resistance profiles of ciprofloxacin and cotrimoxazole, imipenem and tetracycline, tetracycline and ampicillin, and imipenem and azithromycin. Furthermore, the isolates had a multiple antibiotic resistance index of up to 0.47. Antibiotic resistance genes tetA and SHV were found in 69.23% and 50% of relevant antibiotic-resistant Shigella spp. isolates, respectively. The present study suggests that wild waterfowl and migratory birds are reservoirs of MDR Shigella spp., which may have detrimental impacts on One Health components. We suggest keeping these birds under an AMR monitoring program to avoid the possibility of AMR contamination of the environment and its consequences in all health settings.","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"226 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72773756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between Post-Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression.","authors":"Vincentia Trisna Yoelinda, Raden Iis Arifiantini, Dedy Duryadi Solihin, Muhammad Agil, Dedi Rahmat Setiadi, Tulus Maulana, Bambang Purwantara, Yohana Tri Hastuti, Jansen Manansang, Dondin Sajuthi","doi":"10.1155/2023/9982422","DOIUrl":"10.1155/2023/9982422","url":null,"abstract":"<p><p>The role of ex situ conservation facilities or captivity through captive breeding programs is essential in the conservation of the endangered Javan banteng. The development of semen cryopreservation may assist on one side of the conservation plan. However, the male Javan banteng reproductive capability must be considered as it influences the targeted outputs. Studying the potential biomarker for fertility such as osteopontin gene expression is also expected to help predict male fertility. Therefore, this study aimed to analyze the quality of spermatozoa after thawing to help predict the male reproductive capability of Javan banteng. Furthermore, this study investigated the potential role of osteopontin gene expression in male Javan banteng fertility. A positive reinforcement approach was used to accustom the male and female animals as we focused on establishing a collection procedure using neither sedation nor anaesthesia. Semen samples were collected at Taman Safari Indonesia, Bogor, in accordance with the female banteng receptivity. Semen samples were then evaluated and then cryopreserved under field conditions. Our study showed the different predicted reproductive capability of the Javan banteng based on the post-thaw spermatozoa quality, which showed significant differences. The OPN gene showed positive correlations with the progressive motility (<i>r</i> = 0.711, <i>p</i> = 0.048), viability (<i>r</i> = 0.822, <i>p</i> = 0.012), and acrosomal integrity (<i>r</i> = 0.665, <i>p</i> = 0.072) of Javan banteng spermatozoa after thawing. Our study demonstrated the predicted Javan banteng reproductive capability based on various post-thaw spermatozoa variables. This finding is also the first report on the OPN gene potential to be developed as the assessment tool of post-thaw spermatozoa quality of the male Javan banteng. The findings in our study may help give recommendations for future breeding programs, especially in the ex situ conservation sites.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"9982422"},"PeriodicalIF":1.9,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9819950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of <i>Lactobacillus farciminis</i> and <i>Lactobacillus rhamnosus</i> on Growth, Blood Biochemical, and Meat Quality Indicators of Specific Pathogen-Free Broiler Chickens.","authors":"Sabine Eglite, Aija Ilgaza, Lauma Mancevica, Maksims Zolovs","doi":"10.1155/2023/6297068","DOIUrl":"10.1155/2023/6297068","url":null,"abstract":"<p><p>The aim of our study was to evaluate the effects of <i>Lactobacillus farciminis</i> and <i>Lactobacillus rhamnosus</i> on live weight gain, feed consumption indicators, and some metabolic blood biochemical and meat quality indicators of specific pathogen-free Ross 308 broiler chickens. We carried out the study in three trials and included a total of 780 unsexed Ross 308 chickens, which we randomly divided into two groups: the control group (Con, <i>n</i> = 390, basal diet) and the probiotic group (ProL, <i>n</i> = 390, basal diet + a powder consisting of <i>L. farciminis</i> and <i>L. rhamnosus</i> 4 g/10 kg of feed). We raised broilers until day 35. We determined the amount of feed consumed, the average daily weight gain, the feed conversion ratio, the average daily feed intake, and the cumulative feed intake once a week. We collected blood samples from 45 broilers from each group at the end of the study. In addition, we slaughtered 30 broilers from each group by cervical dislocation to obtain a breast muscle sample (without skin) to determine meat quality in these chickens (cholesterol and unsaturated, omega-3, omega-6, omega-9, and saturated fatty acids). Feeding a probiotic mixture containing <i>L. farciminis</i> and <i>L. rhamnosus</i> did not significantly affect the growth and feed intake indicators. Feeding these probiotics significantly lowered the blood serum cholesterol levels but did not provide the expected reduction in meat cholesterol levels. However, feeding a probiotic mixture increased the levels of polyunsaturated fatty acids (omega-3 and omega-6 fatty acids) in the breast meat and decreased saturated fatty acids. To better explain the effect of the combination of lactic acid bacteria (<i>L. farciminis</i> and <i>L. rhamnosus</i>) on the growth and development of broiler chickens in our study, histological and immunohistochemical examinations should be performed.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2023 ","pages":"6297068"},"PeriodicalIF":1.9,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}