Veterinary Medicine International最新文献

{"title":"Radiographic Analysis of the Beef Cattle Digits Slaughtered after 114 Days of Confinement.","authors":"Wanessa Patrícia Rodrigues da Silva, Paulo José Bastos Queiroz, Paulo Henrique Jorge da Cunha, Antônio Dionísio Feitosa Noronha Filho, Emmanuel Arnhold, Juliano José de Resende Fernandes, Kaique de Souza Nascimento, Naida Cristina Borges","doi":"10.1155/2024/5512555","DOIUrl":"10.1155/2024/5512555","url":null,"abstract":"<p><p>Foot disorders are responsible for up to 5% of confinement losses. Identifying the cause of lameness and applying the correct treatment are crucial measures. The possibility of determining the probable origins of digital lesions, their extension, and assistance in cases in which it was not possible to reach a definitive clinical diagnosis demonstrates that the radiographic evaluation provides information of great importance on cattle digits. Thus, this study aimed to investigate possible radiographic changes in the hoof, bone structures, and soft tissues of the digits of Nelore bulls at the end of the confinement period. Regarding the main signs, 100% (<i>n</i> = 24) showed proliferation of enthesophytes and osteophytes and irregular contours, 62.5% (<i>n</i> = 15) enlargement of vascular channels, 37.5% (<i>n</i> = 9) osteolysis, 33.4% (<i>n</i> = 8) gas content in the white line region, 20.84% (<i>n</i> = 5) gas content in the dorsal lamina region, and 4.16% (<i>n</i> = 1) presented palisade periosteal reaction. Ten (41.7%) out of 24 (100%) animals evaluated at the end of the confinement presented lameness, three animals of score three, four animals of score two, and three animals of score one. The higher the score indicating the severity of the signs observed in the visual assessment of the radiographs, the higher the internal angle values (moderate correlation of 0.5 and <i>p</i> < 0.05). A higher prevalence of osteo-proliferative radiographic changes was identified at the end of the 114-day confinement period in the third phalanx of Nellore cattle even in animals that had no lameness.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"5512555"},"PeriodicalIF":1.9,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, Attitude, and Practice of Thai Dairy Farmers on the Use of Antibiotics.","authors":"Niorn Ratanapob, Aksorn Saengtienchai, Theera Rukkwamsuk","doi":"10.1155/2024/5553760","DOIUrl":"10.1155/2024/5553760","url":null,"abstract":"<p><p>Antibiotics have been used regularly in dairy farms by veterinarians; however, they were also used occasionally by farmers without any veterinary prescriptions. Because knowledge, attitude, and practice are important for sustainable antibiotic use, the levels of these aspects among farmers and associated factors should be determined to improve antibiotic use in dairy farming. The study was carried out in 89 Thai dairy farmers, using a structured questionnaire. Data were analyzed using descriptive statistics. The questions were scored and the total scores were calculated for each aspect. Factors associated with knowledge, attitude, and practice scores were identified using the multivariate linear regression. The mean scores for knowledge, attitude, and practice were 62%, 86%, and 78%, respectively. Higher education, participation in a training related to antibiotic use, and being supervised by the Veterinary Teaching Hospital Nong Pho were associated with a higher knowledge score (<i>p</i> < 0.050). Farmers with less experience received a higher attitude score (<i>p</i> = 0.020). Acquiring antibiotic knowledge from other farmers was associated with a lower practice score (<i>p</i> = 0.005). A positive association was found between knowledge and attitude scores (<i>p</i> = 0.021) and practice and attitude score (<i>p</i> < 0.001). Due to the insufficient knowledge on antibiotic use, there is a need to fill the gap to ensure prudent antibiotic use by farmers. Recommendations including training farmers by veterinarian to perceive the prudent antibiotic uses, encouraging young generation with higher education to participate in dairy farming, providing antibiotic use protocols, and decreasing the availability of antibiotics should be implemented to limit overuse by farmers.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"5553760"},"PeriodicalIF":1.9,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Risk Factors of Anaplasmosis in Simmental Cattle in the Peruvian Amazon.","authors":"Hugo Frias, Luis Murga, William Bardales, Vanessa Frias, Segundo Melecio Portocarrero-Villegas, Tatiana Segura Portocarrero, Miguel Arista, José Américo Saucedo-Uriarte","doi":"10.1155/2024/4634440","DOIUrl":"10.1155/2024/4634440","url":null,"abstract":"<p><p>Anaplasmosis, transmitted biologically and mechanically, is one of the most prevalent diseases responsible for high production costs worldwide. In this research, the prevalence and risk factors of anaplasmosis in Simmental cattle raised in the Peruvian Amazon were evaluated. 266 blood samples were collected from bovines of different categories such as calves male, calves females, heifers <1.3 years, heifers >1.3 years, steers, bulls, and cows from the districts of Omia and Molinopampa. The enzyme-linked immunosorbent assay (ELISA) technique was used to detect antibodies against <i>Anaplasma marginale</i>. Of the 266 animals sampled, 67% were positive for A. marginale. A higher prevalence was determined in the district of Omia (99.3%), while in the district of Molinopampa, 28.7% was obtained. A prevalence of <i>A. marginale</i> was recorded in females (67.7%) and in males (64.8%) (<i>p</i> > 0.05). There is a significant association of the disease with the category of cattle, verifying the highest prevalence of <i>A. marginale</i> in calves male, heifer >1.3 years, and bull. The multiple correspondence analysis shows that San Mateo, Puma Marca, Mashuyacu, Primavera, and Los Olivos have a higher prevalence of anaplasmosis, associated with altitude of 1701-2000 m, spray baths and paddock rotation. Anaplasmosis is prevalent in Simmental cattle from the Peruvian Amazon, with a higher incidence in Omia and in females, considering May to August the critical months and the altitude less than 2000 meters above sea level.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"4634440"},"PeriodicalIF":1.9,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Lumpy Skin Disease in Africa: A Systematic Review and Meta-Analysis from 2007 to 2023.","authors":"Balemual Abebaw","doi":"10.1155/2024/9991106","DOIUrl":"10.1155/2024/9991106","url":null,"abstract":"<p><strong>Background: </strong>Lumpy skin disease (LSD) is an economically important, transboundary viral disease of cattle caused by LSD virus. This systematic review and meta-analysis are concerned with summarizing the status of lumpy skin disease in Africa.</p><p><strong>Methods: </strong>Literature searches between 2007 and 2023 were conducted using different database searches: Google, Google Scholar, PubMed, and Science Direct, and then the required information were extracted. A random effect model was used to pool estimates of prevalence at 95% CI. Publication bias was assessed by applying funnel plots.</p><p><strong>Results: </strong>The pooled estimated prevalence of LSD was 54% (95% CI: -0.09-1.18), with individual study prevalence ranging from 6 to 89%. Subgroup analysis showed that the random pooled prevalence of LSD was 28% (95% CI: -1.48-2.03) in Ethiopia, 37% (95% CI: -3.79-4.52) in Uganda, 88% (95% CI: -0.11-1.28) in Zimbabwe, and 58% (95% CI: -6.96-8.72) in Egypt. No single study was documented from western and northern Africa.</p><p><strong>Conclusion: </strong>The estimated pooled LSD prevalence was investigated as high and indicated nonsignificant variation among study countries. Thus, vaccination of cattle, quarantine, and biting insect control should be applied to control the disease.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"9991106"},"PeriodicalIF":3.1,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141311810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian Rhythms Fluctuate the Treatment Effects of Intravesical Treatments on Rat Urinary Frequency Models.","authors":"Tomofumi Watanabe, Takuya Sadahira, Yusuke Tominaga, Yuki Maruyama, Naoya Nagasaki, Takanori Sekito, Kohei Edamura, Toyohiko Watanabe, Motoo Araki, Masami Watanabe","doi":"10.1155/2024/6505595","DOIUrl":"10.1155/2024/6505595","url":null,"abstract":"<p><strong>Objectives: </strong>It is still not clear how the intravesical instillation of drugs affects rat urinary frequency. This study aimed to examine the dynamics of intravesical treatments' treatment effect on rat urinary frequency models by real-time and extended monitoring using a novel continuous urination monitoring system.</p><p><strong>Methods: </strong>Nine eleven-week-old female Wistar rats were divided into three groups to receive intravesical instillation of 0.1% acetic acid (AA), 1.0% AA, or phosphate-buffered saline (PBS). Thirty minutes later, these drugs were voided, and rats were moved to a continuous urination monitoring system, UM-100. UM-100 monitored rat urination quantitatively and continuously for 24 hours. Rats were then euthanized, and histopathologic examinations using a damage score validated the severity of bladder inflammation. We used nine additional rats to determine the treatment effect of various drugs against the urinary frequency. These rats were also treated with 1.0% AA in the same way and divided into three groups (<i>n</i> = 3 each) to receive intravesical instillation of lidocaine, silver nitrate (AgNO₃), or dimethyl sulfoxide (DMSO), respectively. Thirty minutes later, rats were catheterized again and moved to the UM-100, and their voiding was monitored for 24 hours.</p><p><strong>Results: </strong>Intravesical instillation of AA increased the urinary frequency and decreased the mean voided volume (VV) in a concentration-dependent manner, with statistical significance at a concentration of 1.0% (urinary frequency; <i>p</i>=0.0007, mean VV; <i>p</i>=0.0032, respectively) compared with PBS. Histopathological analysis of these models demonstrated a significantly higher damage score of bladder mucosa in both 0.1% AA and 1.0% AA compared with PBS, with the severity in concordance with the clinical severity of urinary frequency (0.1% AA: <i>p</i> < 0.0001, 1.0% AA: <i>p</i> < 0.0001). Moreover, intravesical instillation of lidocaine, AgNO₃, and DMSO decreased the urinary frequency. Continuous monitoring with UM-100 also demonstrated that the treatment effect of these intravesically instilled drugs occurred only at night.</p><p><strong>Conclusions: </strong>The extended monitoring of rat urination by UM-100 revealed a significant fluctuation in the treatment effect of intravesically instilled drugs between day and night. These findings may help establish novel therapies for urinary frequency.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"6505595"},"PeriodicalIF":3.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11150046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the Transmission, Host Range, Genomics, Vaccination, and Current Epidemiology of the Monkeypox Virus.","authors":"Yusha Araf, Jannatul Ferdous Nipa, Sabekun Naher, Sumaiya Tasnim Maliha, Hasanur Rahman, Kazi Ifthi Arafat, Mohammad Raguib Munif, Md Jamal Uddin, Nurejunnati Jeba, Sukumar Saha, Jingbo Zhai, S M Nazmul Hasan, Mengzhou Xue, Md Golzar Hossain, Chunfu Zheng","doi":"10.1155/2024/8839830","DOIUrl":"10.1155/2024/8839830","url":null,"abstract":"<p><p>This review delves into the historical context, current epidemiological landscape, genomics, and pathobiology of monkeypox virus (MPXV). Furthermore, it elucidates the present vaccination status and strategies to curb the spread of monkeypox. Monkeypox, caused by the <i>Orthopoxvirus</i> known as MPXV, is a zoonotic ailment. MPXV can be transmitted from person to person through respiratory droplets during prolonged face-to-face interactions. While many cases of monkeypox are self-limiting, vulnerable groups such as young children, pregnant women, and immunocompromised individuals may experience severe manifestations. Diagnosis predominantly relies on clinical presentations, complemented by laboratory techniques like RT-PCR. Although treatment is often not required, severe cases necessitate antiviral medications like tecovirimat, cidofovir, and brincidofovir. Vaccination, particularly using the smallpox vaccine, has proven instrumental in outbreak control, exhibiting an efficacy of at least 85% against mpox as evidenced by data from Africa. Mitigating transmission requires measures like wearing surgical masks, adequately covering skin lesions, and avoiding handling wild animals.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"8839830"},"PeriodicalIF":3.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11150048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxoplasmosis and Chlamydophilosis in Small Ruminant Farms in Cameroon: Knowledge, Attitudes, Practices, and Perception of Zoonotic Risks of Farmers.","authors":"Roland Nankam Chimi, Justin Kouamo, Michel Alain Simo Kouam, Muller Dzousse Fotsac, Raphael Chermapi Dembeng, Armelle Prudence Kouengoua Kouengoua, Josué Simo Louokdom, Ferdinand Ngoula","doi":"10.1155/2024/2946764","DOIUrl":"https://doi.org/10.1155/2024/2946764","url":null,"abstract":"<p><p>Zoonotic abortive diseases represent a significant health and economic risk for national public health. This cross-sectional survey was conducted from April to October 2021 among 200 selected small ruminant farmers in the three northern regions of Cameroon. Data collection was done through questionnaires administered by exchange with the herder, and responses were coded and recorded on an Excel spreadsheet. The data were then analyzed with R software, version 2.13.0. An ANOVA test was used to assess significant differences in mean of Knowledge, Attitudes, Practices, and Perception of zoonotic risks (KAPP) scores between regions. Pearson correlations were used to calculate the association between KAPP scores within regions. Small ruminant farmers surveyed had low mean scores for knowledge of abortive toxoplasmosis and chlamydophilosis (0.1 ± 0.2), desirable attitude (0.32 ± 0.07), appropriate practice (0.36 ± 0.13) in managing abortion, and positive perception of zoonotic risks of toxoplasmosis and chlamydophilosis in small ruminants (0.12 ± 0.33), respectively. KAPP was significantly (<i>P</i> < 0.01) and positively associated with knowledge (<i>r</i> = 0.98) and risk perception (<i>r</i> = 0.99). However, attitudes (<i>r</i> = 0.06), practices (<i>r</i> = 0.05), and risk perception of toxoplasmosis (<i>r</i> = 0.07) and chlamydophilosis (<i>r</i> = 0.08) were not associated with farmers' knowledge. This study revealed significant knowledge gaps, low levels of desired attitudes, and high-risk behavioral practices. These results therefore call for capacity building of health professionals and farmers to better integrate the One Health concept in the management of neglected zoonotic diseases.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"2946764"},"PeriodicalIF":1.9,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11390209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of <i>Anaplasma phagocytophilum</i> in Small Mammals and Infesting Ticks in Laikipia County, Kenya.","authors":"Erick Titus Mosha, Joseph K N Kuria, Moses Otiende, Isaac Lekolool","doi":"10.1155/2024/5575162","DOIUrl":"https://doi.org/10.1155/2024/5575162","url":null,"abstract":"<p><p>Anaplasmosis is a set of disease conditions of various mammals caused by bacteria species of the genus <i>Anaplasma</i>. These are sub-microscopic, Gram-negative, obligate intracellular pathogens that infect both vertebrate and invertebrate hosts. Significant species that infect domestic and wildlife animals include <i>Anaplasma marginale, Anaplasma ovis</i>, <i>Anaplasma mesaeterum, Anaplasma platys</i>, and <i>Anaplasma phagocytophilum</i>. Although <i>A</i>. <i>phagocytophilum</i> has a widespread distribution, there are only a few epidemiological reports from sub-Saharan Africa. This study focused on molecular detection and characterization of <i>A. phagocytophilum</i> in small mammals and their infesting ticks in Laikipia County, Kenya. A total of 385 blood and 84 tick archival samples from small mammals (155 females and 230 males) were analyzed. The blood samples were subjected to a nested PCR-HRM melt analysis using species-specific primers to amplify the 16S ribosomal RNA genes. The ticks were also subjected to nested PCR-HRM involving 16S rRNA gene primers. <i>Anaplasma phagocytophilum</i> DNA was detected in 19 out of 385 samples using species-specific 16S rRNA gene primers giving a prevalence of 4.9% for <i>A. phagocytophilum</i>. Analysis of the tick's samples using 16S rRNA gene species-specific primers also detected <i>A. phagocytophilum</i> in 3 samples from <i>Haemaphysalis leachi</i> ticks (3/84) equivalent to prevalence of 3.6%. Sequencing of 16S rRNA PCR products confirmed <i>A. phagocytophilum</i> in small mammals and ticks' samples. Phylogenetic analysis of the haplotype from this study demonstrated a close ancestral link with strains from <i>Canis lupus familiaris, Alces alces, Apodemus agrarius</i>, and ticks (<i>Haemaphysalis longicornis</i>) reported in Europe, China, and Africa. Comparison was also made with a known pathogenic <i>A</i>. <i>phagocytophilum</i> variant HA and a nonpathogenic variant 1 that were clustered into a distinctive clade different form haplotypes detected in this study. All the haplotype sequences for <i>A. phagocytophilum</i> from this study were submitted and registered in GenBank under the accession numbers OQ308965-OQ308976. Our study shows that small mammals and their associated ticks harbor <i>A. phagocytophilum</i>. The vector competence for <i>H</i>. <i>leachi</i> in <i>A. phagocytophilum</i> transmission should further be investigated.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"5575162"},"PeriodicalIF":3.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11098608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of Monoclonal Antibodies against CBPP Antigen with the End Goal of Developing an ELISA Kit.","authors":"Lorato Ramathudi-Dunbar, Emmanuel Awosanya, Sanne Bodjo Charles, Ethel Chitsungo, Cisse Rahamatou Moustapha Boukary, Nick Nwankpa, Hassen Gelaw, Yebechaye Tessema, Gelagay Melesse A, Richard Rayson Sanga, Adorbley Bright, Jean de Dieu Baziki","doi":"10.1155/2024/6901355","DOIUrl":"10.1155/2024/6901355","url":null,"abstract":"<p><p>Contagious bovine pleuropneumonia (CBPP) is an infectious and contagious bacterial respiratory disease that affects cattle with significant economic losses to the African animal industry. The use of ELISA kits based on monoclonal antibodies (mAbs) will aid in quick and precise diagnosis of CBPP, contributing to disease control and prevention in cattle. Thus, this research aims to develop and evaluate monoclonal antibodies against CBPP (T1/44) antigen for use in ELISA kits for CBPP diagnosis. Hybridoma technology was used to develop monoclonal antibodies that recognize and bind to the CBPP (T1/44) antigen. The antibody-secreting hybridomas were produced after immunizing mice with purified CBPP antigens. The hybridomas were screened for high sensitivity, specificity, and liking to the antigen. The selected mAbs were assessed for sensitivity and specificity against CBPP antigen using different immunoassays, dot-blot, ELISA, and mouse mAb isotyping. The monoclonal antibodies were profoundly specific, with a higher hindrance to CBPP antigen (<0.50 OD) while lacking cross-reactivity to other antigens. The monoclonal antibodies could distinguish CBPP antigen at low concentrations, showing their high sensitivity (>80% PI). The isotyped mAbs of intrigued appeared to have a place in the IgG class. These identified monoclonal antibodies can be utilized to develop an ELISA kit for CBPP diagnosis, which would give a fast, precise, and cost-effective strategy for screening and checking CBPP in cattle herds.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"6901355"},"PeriodicalIF":3.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11093687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the Antioxidant Hydroxytyrosol on the Quality of Post-Thawed Stallion Semen.","authors":"Yousef M Alharbi, Mohamed Ali, Mohammed S Alharbi","doi":"10.1155/2024/6558480","DOIUrl":"10.1155/2024/6558480","url":null,"abstract":"<p><p>The objective of this study was to investigate the impact of including hydroxytyrosol (HT) antioxidant on the viability of sperm after the processes of cooling and freezing. HT antioxidants were used in the HF-20 extender at concentrations of 1.25, 2.5, 5, and 10 <i>μ</i>g/ml. The HF-20 extender was a basic extender and was used for the control group. The post-thawed semen exhibited significantly higher total motility in the 2.5 HT and 10 HT treatment groups than the control group. The straight line velocity (VSL) of the 2.5 HT group exhibited a significantly high value compared with the control group. However, the average path velocity (VAP), linearity (LIN), straightness index (STR), and wobble (WOB) revealed identical findings across all groups. The findings of the analysis of HOST, normal morphology, major abnormalities, and minor abnormalities revealed that there were no significant differences between the HT groups and the control groups. Nevertheless, the use of HT antioxidant for freezing semen led to a notable enhancement (<i>p</i> < 0.05) in both acrosome integrity and vitality tests when compared to the control group. In this case, the lower quantities of HT (1.25 and 2.5 <i>μ</i>g/ml; <i>p</i> < 0.05) preserve the DNA fragmentation compared with the 5 HT, 10 HT, and control groups. In conclusion, the HT antioxidant has shown the capacity to enhance the quality of frozen-thawed spermatozoa and positively influence the viability and integrity of DNA inside the frozen-thawed spermatozoa. Additional research should be conducted to assess the fertility potential of cryopreserved stallion semen.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2024 ","pages":"6558480"},"PeriodicalIF":3.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}