Veterinary Medicine International最新文献

{"title":"Comparative Transcriptome Analysis of Hens' Livers in Conventional Cage vs. Cage-Free Egg Production Systems.","authors":"María Paula Herrera-Sánchez, Roy Rodríguez-Hernández, Iang Schroniltgen Rondón-Barragán","doi":"10.1155/vmi/3041254","DOIUrl":"10.1155/vmi/3041254","url":null,"abstract":"<p><p>Different conditions of production systems including stocking density, thermal conditions, and behavior restriction can have a significant detrimental effect on the health and performance of laying hens. The conventional cage system is one of the systems that have been reported to cause stress problems in birds, due to social and behavioral stress. Emerging technologies have facilitated a deeper understanding of animal responses to various scenarios and can be an additional tool to conventional ones to assess animal welfare, where transcriptomic analysis has the potential to show the genetic changes that occur in response to stress. According to this, the aim of this work was to characterize the liver transcriptome of hens housed under two egg production systems (conventional cage and cage-free). Liver tissue from Hy-Line Brown hens housed in conventional cage (<i>n</i> = 3) and cage-free (<i>n</i> = 3) production systems at week 80 of age was processed using the Illumina platform to identify differentially expressed genes with a padj < 0.05. Regarding the differentially expressed genes, 138 genes were found, of which 81 were upregulated and 57 downregulated. Some of the genes of interest were <i>TENM2, GRIN2C,</i> and <i>ACACB</i>, which would indicate greater fat synthesis in the liver of caged hens. The enriched KEGG pathways were DNA replication and the cell cycle. In conclusion, it was identified that the cage production system may influence DNA replication and the cell cycle since the genes related to these terms were found suppressed, which would indicate cellular instability.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"3041254"},"PeriodicalIF":1.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Bovine Fasciolosis, Financial Losses and Risk Factors Associated with the Disease in Lira and Gulu Districts, Northern Uganda.","authors":"Anthony Ogwal, Daniel Micheal Okello, Tony Aliro, David Okello Owiny, Elly Kurobuza Ndyomugyenyi","doi":"10.1155/vmi/7757654","DOIUrl":"10.1155/vmi/7757654","url":null,"abstract":"<p><p>Bovine fasciolosis (BF) is a parasitic disease of cattle that causes significant economic impacts to cattle farmers. The physical loses include weight loss, drop in productivity, liver condemnation and mortalities. This study assessed the prevalence, financial losses and risk factors associated with the disease in Lira and Gulu Districts, northern Uganda. A cross-sectional study of 720 slaughter cattle from the abattoirs was conducted by macroscopic examination of the animals and carcasses during routine slaughter. In the farms, 120 rectal faecal samples were taken to a laboratory for <i>Fasciola</i> egg detection by simple microscopy, while risk factors were assessed by administering a questionnaire to farm owners. Prevalence of BF was highest (38%) within cattle in the age group of 1-3 years and lowest (18%) in those over 5 years. The overall BF prevalence was 48% and 26% by postmortem examination and coprology, respectively. The cattle body condition had a significant association (<i>p</i> < 0.01) with prevalence of BF, in which emaciated cattle were most affected (59%), while fat ones had the least prevalence (15%). The annual financial loss due to BF infection was USD 89,099. The major risk factors associated with the disease at the farm level were communal grazing in swampy areas (82%) and watering cattle in swamps (71%). Farms where the source of drinking water was swamp water had higher chances (<i>p</i> < 0.05) of their cattle having BF. An integrated approach using routine deworming programs, improvement of cattle management practices and control of snail intermediate hosts is recommended to effectively control the spread of BF.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"7757654"},"PeriodicalIF":1.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Husbandry Practices, Productive Performances, and Egg Quality Traits of SassoT44 and Koekoek Chicken Breeds in Urban and Rural Production Systems of Jama District, South Wollo Zone, Ethiopia.","authors":"Mesfin Fasil, Aleme Asresie, Yilkal Tadele","doi":"10.1155/vmi/3025355","DOIUrl":"https://doi.org/10.1155/vmi/3025355","url":null,"abstract":"<p><p>Chicken production is an integral part of livestock farming in Ethiopia. Despite the local chicken ecotypes, the country introduces different exotic chicken breeds. This study aimed to assess husbandry practices, egg productive performances, and egg quality of SassoT44 and Koekoek chicken breeds in urban and rural production systems of Jama district. Purposive and random sampling techniques were employed to select 188 smallholder farmers for an interview. Data were collected on household characteristics, chicken ownership, husbandry practices, egg production performances, and egg quality traits. The proportion of males (59.3%) involved in chicken rearing is higher than that of females (40.7%). The results of the study indicated that majority of respondents (167) owned less number of SassoT44 and Koekoek chickens (5-10). Most of the respondents provided house and additional feed supplement to their chicken. The major feed sources were scavenging with supplements. The mean egg production performances of SassoT44 and Koekoek chicken breeds were 239.3 and 193 eggs/hen/year, respectively. Significantly higher (<i>p</i> < 0.001) numbers of eggs per hen/year (252.6 for SassoT44 and 198 for Koekoek) were recorded in the urban production system than in the rural production system (226 for SassoT44 and 187.5 for Koekoek). Egg weight was influenced by both chicken breed and production system. Higher (<i>p</i> < 0.001) weight of egg (56.7 g) was recorded for SassoT44 than that of Koekoek (51.1 g). SassoT44 was superior for a mean value of egg weight, width, albumen weight, yolk height, and yolk color (YC) than Koekoek. Most of the egg quality parameters have positive correlations. Sasso performs better in egg production and most egg quality traits in the study area.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"3025355"},"PeriodicalIF":1.9,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Substituting Concentrate Mix With Sweet Potato Vines on Growth Performances and Carcass Components of Yearling Rams and Its Potential in Mitigating Methane Production.","authors":"Assefa Tadesse, Natascha Titze, Markus Rodehutscord, Aberra Melesse","doi":"10.1155/vmi/1054348","DOIUrl":"https://doi.org/10.1155/vmi/1054348","url":null,"abstract":"<p><p>This study was conducted to investigate the substitution effect of the concentrate mix (CM) with sweet potato vine (SPV) on growth performance, carcass characteristics of rams, and methane (CH₄) production. Forty yearling rams with an initial body weight of 18.5 ± 0.7 kg were randomly distributed into five treatment diets with eight rams each. A CM of 385 g/head/d was supplied to the control group (SPV0), and diets were formulated to replace the CM of the control diet with SPV at 10% (SPV10), 20% (SPV20), 30% (SPV30), and 40% (SPV40). After 82 days, four rams from each treatment were randomly selected and slaughtered. The CH₄ production was determined from 24-h <i>in vitro</i> gas production (GP), and the digestible organic matter (dOM) and metabolizable energy (ME) were estimated from the GP. Data were analyzed using the one-way ANOVA and orthogonal polynomial contrast functions. Results indicated that the contents of the crude protein (CP) and ash in SPV were 258 and 148 g/kg DM, respectively. The substitution of CM with SPV in the <i>in vivo</i> trial did not affect body weight gain, feed intake, and feed conversion ratio (FCR). Rams fed with the SPV0 diet had higher (<i>p</i> < 0.05) hot carcass weight than those reared in the SPV10, SPV20, and SPV40 diets. The slaughter weight and dressing percentage showed significant linear and quadratic effects. Moreover, significant linear and cubic trends were noted for hot carcass, thorax, foreleg, and hindleg. The SPV0 diet had the highest GP (<i>p</i> < 0.001) compared to the SPV30 and SPV40 diets. The SPV40 diet and the pure SPV had the lowest CH₄ production (<i>p</i> < 0.001). The SPV0 diet showed similar ME with SPV10 and SPV20 diets but was higher (<i>p</i> < 0.001) than the SPV30 and SPV40 diets and pure SPV. The dOM did not differ between treatment diets. Similarly, the dOM of SPV did not differ (<i>p</i> > 0.05) from that of the SPV30 and SPV40 diets. In conclusion, replacing CM with SPV significantly reduced the <i>in vitro</i> CH₄ production without affecting voluntary feed intake, weight gain, some carcass components, and dOM.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"1054348"},"PeriodicalIF":1.9,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Immunogenicity of Recombinant Adeno-Associated Virus-Vectored African Swine Fever Virus Antigens.","authors":"Antonina Galeeva, Marina Efimova, Gennadiy Frolov, Nail Khammadov, Almaz Hisamutdinov, Lenar Garipov, Danil Mingaleev, Rustam Ravilov","doi":"10.1155/vmi/9191117","DOIUrl":"https://doi.org/10.1155/vmi/9191117","url":null,"abstract":"<p><p>Despite the significant global economic damage caused by African swine fever (ASF) and ongoing developments in the field of specific prevention tools development, safe and effective vaccines are still missing. A critical factor hindering the development of ASF vaccines is the lack of sufficient data on the pathogenesis of the virus, as well as a deep understanding of the virus' evasion strategies from the innate immune system. Of particular interest in the design of candidate vaccines are viral vectors, especially-adeno-associated virus (AAV), which is widely used in gene therapy and is capable of long-term transgene expression in vivo. This study assessed the safety and immunogenicity of recombinant AAV serotype 2 (rAAV2), into the genome of which the ASF virus <i>B646L</i> (p72)<i>, E183L</i> (p54)<i>, CP530R</i> (pp60), and <i>CP204L</i> (p30) immunodominant genes are integrated. The study design included immunization of pigs with monocistronic and bicistronic constructs based on rAAV2 in different regimens, assessment of the safety and tolerability of a laboratory sample of the vaccine, the biochemical and hematological status of the animals, as well as indicators of humoral and cellular immunity. It was found that rAAV2s in immunizing doses no more than 10 × 10¹¹ viral particles have satisfactory tolerability, promote the formation of virus-specific antibodies that remain at a high level at least until the 180 days of the experiment. It has been proven that the use of bicistronic constructs makes it possible to achieve a similar immune response as when introducing a cocktail of monocistronic constructs, which allows to reduce the vector load on the animal's body. Thus, rAAV2 is a promising platform for the construction of a candidate vaccine against ASF, as it is biologically safe and activates the humoral and cellular immune response, which is extremely important for the formation of a protective immunity.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"9191117"},"PeriodicalIF":1.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal Disease in Dogs From Mexico: Description of Most Commonly Affected Teeth and Associated Factors.","authors":"Ana G Villegas-Ferre, Eduardo Gutierrez-Blanco, Víctor M Martínez-Aguilar, Gandhi S Hernandez-Chan, Matilde Jiménez-Coello, Antonio Ortega-Pacheco","doi":"10.1155/vmi/6628061","DOIUrl":"https://doi.org/10.1155/vmi/6628061","url":null,"abstract":"<p><p>With the objective to evaluate the prevalence of periodontal disease (PD) and monitor the most affected teeth in dogs under the subtropical conditions in Mexico, 184 randomly selected dogs underwent a periodontal examination. Of the evaluated dogs, 78.8% showed some degree of gingivitis most of them (76.6%) with a moderate index, and 30.4% presented some degree of PD being most of them of a mild degree. Old age and small size dogs were more prone to develop PD as reported elsewhere. Higher mobility index and furcation were seen in maxillary teeth 108 and 208 and mandibular teeth 308 and 408, but some other teeth were involved in less proportion. On probing, bleeding was observed in maxillary teeth 202, 11, 102, and 202 and mandibular teeth 309 and 409. Dental plaque and calculus were more frequent in maxillary teeth 202 and 102 and mandibular teeth 309, 310, and 409. Finally, dental loss was observed with more frequency in all maxillary incisors (102, 201, and 202) and molars 109 and 209; mandibular molars 411 and 311 were more prompt to be losed. This study demonstrates the high prevalence of gingivitis and development of PD in dogs in subtropics in Mexico and reveals the predisposition of some maxillary and mandibular teeth to develop PD and consequently their loss. The clinical implications of the study indicate that special attention should be paid to these teeth to be checked when the dogs come for consultation, during brushing and in dental prophylaxis.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"6628061"},"PeriodicalIF":1.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Vietnamese coriander</i> Powder on Growth, Body Composition, Hematology, and Immune-Related Gene Expression in Nile Tilapia.","authors":"Apisara Honghirun, Rungrawee Thongdon-A, Niran Aeksiri, Kumrop Ratanasut, Wilasinee Inyawilert, Gen Kaneko, Anurak Khieokhajonkhet","doi":"10.1155/vmi/1253764","DOIUrl":"https://doi.org/10.1155/vmi/1253764","url":null,"abstract":"<p><p>Dietary supplementation of plant herbs into aquafeed is recommended for intensive and sustainable aquaculture practices. This study has investigated the effect of <i>Persicaria odorata</i> (PO) leaf powder on growth, feed utilization, whole-body composition, hematology, and immune-related gene expression in Nile tilapia (<i>Oreochromis niloticus</i>). Fish (∼7.77 ± 0.01 g/fish) were randomly allocated into four treatment groups, each with three replicates. They were fed with dietary supplementation of PO at concentrations of 0, 15, 30, and 50 g/kg (termed PO0-PO50) for 10 weeks. Results indicated that the final body weight, weight gain, and specific growth rate were significantly higher at all PO supplementation levels compared to the control, with the highest value in the PO30 group. Similarly, the protein efficiency ratio and protein productive value in all PO groups were significantly higher than those of the control group. Red blood cells, white blood cells, and hemoglobin levels showed quadratic increases in the PO groups. In addition, total protein, globulin, and high-density lipoprotein cholesterol were linearly and quadratically increased with increasing PO levels, but alanine aminotransferase activity was linearly and quadratically decreased. Furthermore, dietary PO supplementation linearly decreased triglyceride and lipoprotein cholesterol levels, with the lowest levels found in the PO15 group. The expression of genes related to immunity showed that dietary supplementation of PO significantly increased the expression of proinflammatory factors (IL-1β and TNF-α), anti-inflammatory (IL-10 and TGF-β), and HSP70. In addition, glucose and cortisol levels decreased in all PO-supplemented groups, with the lowest levels found in the PO50 and PO30 groups, respectively. These findings showed that a dietary intervention with PO could improve growth, feed utilization, hematological parameters, upregulating genes related to immunity, and decreasing stress markers in Nile tilapia. Consequently, the utilization of PO at a dosage of 30 g/kg could contribute to the sustainable development of the aquaculture sector.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"1253764"},"PeriodicalIF":1.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable Stents in Companion Animals: A Systematic Scoping Review.","authors":"Daiana R Cardoso, Alexandre Barros, André Meneses, João F Requicha","doi":"10.1155/vmi/6405530","DOIUrl":"https://doi.org/10.1155/vmi/6405530","url":null,"abstract":"<p><p><b>Objective:</b> Describe the existing scientific literature regarding the feasibility and behavior of biodegradable stents (BDSs) in companion animals. <b>Study Design:</b> Systematic scoping review. <b>Methods:</b> A literature search was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR) and followed the recommendations of the Cochrane Collaboration. The search was performed by using PubMed, Web of Science, and Scopus databases, focusing on BDS usage in companion animals' urinary, respiratory, and digestive systems including choledochal duct. <b>Results:</b> In total, 233 articles were identified but only 21 fulfilled the inclusion criteria. Ninety percent (<i>n</i> = 19) of the investigation was conducted in animal models aiming translational to humans, and only two studies involved clinical cases. Regarding application, 42.9% was focused on the urinary system, 19% assessed vascular stenting, 14.3% esophageal stenting, 19% choledochal duct stenting, and 4.8% tracheal stenting. Polylactic acid (PLA) was the most used biomaterial. <b>Conclusion:</b> This review summarized the use of BDS in companion animals, highlighting that most studies were conducted in animals without clinical disease, with most reporting a low incidence of self-limiting complications. <b>Clinical Significance:</b> This review underscores the potential impact of BDS on companion animals. Further research is necessary to explore BDS's full potential in small animal medicine.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"6405530"},"PeriodicalIF":1.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A-Kinase Anchor Protein 4 (proAKAP4): Protein Molecule-Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen-Thawed Sperm Quality.","authors":"Berlin Pandapotan Pardede, Erif Maha Nugraha Setyawan, Syahruddin Said, Asmarani Kusumawati, Bambang Purwantara, Mulyoto Pangestu, Erdogan Memili","doi":"10.1155/vmi/8367714","DOIUrl":"10.1155/vmi/8367714","url":null,"abstract":"<p><p>The precursor protein of A-kinase anchor protein 4 (proAKAP4), which is abundant in the fibrous sheath of sperm, plays an essential role in sperm motility and has been developed into one of the candidate molecular-based fertility markers. This study aimed to identify proAKAP4 in the sperm of Indonesian dairy bulls and to investigate its correlation with various sperm quality characteristics. The findings are expected to be applied and developed to optimize the use of superior dairy bulls. Frozen semen from 20 Indonesian dairy bulls were used, which had previously been classified based on their fertility levels into two groups: high fertility (HF) and low fertility (LF). Analysis of frozen-thawed sperm quality, such as progressive and total motility, sperm kinematics, membrane integrity, mitochondrial membrane potential, acrosome integrity, and capacitation status, was carried out in both groups. ProAKAP4 protein in frozen-thawed sperm was analyzed using the Bull 4MID Kit with the ELISA technique. Each dairy bull showed varying proAKAP4 abundance (<i>p</i> < 0.05) from one another. Frozen-thawed sperm quality in HF bulls was higher (<i>p</i> < 0.05) than in LF bulls, especially in total and progressive motility, sperm kinematics, membrane integrity, mitochondrial membrane potential, acrosome integrity, and noncapacitated sperm. The parameters of capacitated and acrosome-reacted sperm showed the opposite results, where the quality in LF bulls was higher (<i>p</i> < 0.05) than in HF bulls. The results of proAKAP4 identification showed that the protein abundance in HF bulls was higher (<i>p</i> < 0.05) than in LF bulls. Correlation analysis confirmed a strong association (<i>p</i> < 0.05) between proAKAP4 levels and sperm fertility rate. The proAKAP4 protein has great potential to be developed and applied as a marker for determining the fertility of dairy bulls and for selecting superior dairy bulls.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"8367714"},"PeriodicalIF":1.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Results of Intestinal Villi of Laying Hens Exposed With Avian Pathogenic <i>Escherichia coli</i> (APEC) After Giving Citric Acid and Dextrose.","authors":"Sunaryo Hadi Warsito, Mirni Lamid, M Anam Al-Arif, Herry Agoes Hermadi, Emy Koestanti Sabdoningrum, Siti Rani Ayuti, Yan-Der Hsuuw","doi":"10.1155/vmi/6623764","DOIUrl":"10.1155/vmi/6623764","url":null,"abstract":"<p><p>The condition known as colibacillosis is still very common in Indonesia, which means that laying hens affected by it are unable to achieve their peak egg production phase. Instead, their egg production is delayed and more susceptible to infection by other diseases. The goal of this study is to determine if the mixture of citric acid and dextrose can inhibit the growth of Avian Pathogenic <i>Escherichia coli</i> (APEC) bacteria in laying hens, ultimately leading to the control of colibacillosis cases in Indonesia. A total of 240 laying hen heads in all were split up into 6 treatments, each with 40 replications.The group received the following treatments: T0 is a treatment for laying hens free of APEC infection and they are given no drink that contains any mixture of citric acid and dextrose. T0 (-) is a treatment for laying hens free of APEC infection but a drink mixture of citric acid and dextrose is administered at a dose of 1 g/2.5 L of drinking water. T0 (+) is a treatment for laying hens infected with APEC up to 2 mL/head orally (3 × 10⁸ CFU/mL) and given a drink without the mixture of citric acid and dextrose. T1 is a treatment for laying hens infected with APEC up 2 mL/head orally (3 × 10⁸ CFU/mL) and given a mixture of citric acid and dextrose at a dose of 1 g/1.25 L of drinking water. T2 is a treatment for laying hens infected with APEC up to 2 mL/head orally (3 × 10⁸ CFU/mL) and given a mixture of citric acid and dextrose at a dose of 1 g/2.5 L of drinking water. T3 is a treatment for laying hens infected with APEC up to 2 mL/head orally (3 × 10⁸ CFU/mL) and given a mixture of citric acid and dextrose at a dose of 1 g/3.75 L of drinking water. The results of this study stated that the mixture of citric acid and dextrose showed a significant decrease in the appearance of the intestinal villi of laying hens, because the results were actually less good compared to the treatment infected with APEC. It is suspected that the dose given may still be excessive in concentration. This can be seen from the dose with the highest concentration range (T1) to the lowest (T3) which showed an image of intestinal villi that had a tendency to improve. Therefore, further research and studies are needed regarding the use of a mixture of citric acid and dextrose in laying hens infected with APEC with a lower dose.</p>","PeriodicalId":23503,"journal":{"name":"Veterinary Medicine International","volume":"2025 ","pages":"6623764"},"PeriodicalIF":1.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}