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[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen]. [利用传染性腹膜炎猫腹膜液中的γ -球蛋白进行TGE抗原的免疫荧光检测]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
G Ignatov, A Tunkara, M Ignatova
{"title":"[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].","authors":"G Ignatov,&nbsp;A Tunkara,&nbsp;M Ignatova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 8","pages":"44-7"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13598132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Electron microscopy research on cytopathic rotaviruses in cell cultures]. [细胞培养中细胞病变轮状病毒的电镜研究]
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
G K Georgiev, S Bozhkov
{"title":"[Electron microscopy research on cytopathic rotaviruses in cell cultures].","authors":"G K Georgiev,&nbsp;S Bozhkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studied was the virus reproduction in cell cultures of MA-104 of the referent rotavirus strain Lincoln, and of the isolated from calves rotavirus Trebich 248/82 and Dolno-sakhrane 39/82. Observed were the following main morphologic structures: electron-dense oval fractions, fractions of with single and double membrane, fractions with supercapsular membrane and electronic-densely granulated zones--viroplasms. There were not established considerable differences between the referent and the isolated from calves rotavirus strains in our country.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 10","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13972983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effect of tuberculin and levamisole on the immune response after vaccinating calves against para-influenza and Salmonella infections]. [结核菌素和左旋咪唑对犊牛接种副流感和沙门氏菌感染后免疫反应的影响]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
I E Ivanov, R Arsov, I Simov, I Dimov, I Sizov
{"title":"[Effect of tuberculin and levamisole on the immune response after vaccinating calves against para-influenza and Salmonella infections].","authors":"I E Ivanov,&nbsp;R Arsov,&nbsp;I Simov,&nbsp;I Dimov,&nbsp;I Sizov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studied was the effect of tuberculin and levamizole on the immune response following vaccination of calves against parainfluenza and Salmonella infections. It was found that in calves that had colostrum antibodies against parainfluenza the immunogenic stimulation with the two agents first led to a drop of the total protein and of the antibody titer of the blood serum; in later periods the antibody titer grew severalfold. The stimulation with tuberculin and levamizole in the case of Salmonella infection substantiated more abrupt humoral immune response as against the case of animals that were not stimulated. More effective was the stimulation when lemvamizole was applied on the 7th day following vaccination. It was established that both tuberculin and levamizole stimulated the phagocytic activity of the blood of calves vaccinated against a parainfluenza virus and did not influence essentially the same activity in calves vaccinated against Salmonella infection. The amount of erythrocyte-rosette forming cells (E-POK) also remained visibly unchanged by the effect of tuberculin and levamizole with the use of the two vaccines.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 4","pages":"43-9"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13957885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Rotavirus enteritis in buffaloes]. [水牛的轮状病毒肠炎]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
L Muniiappa, G K Georgiev, Kh E Kharalambiev
{"title":"[Rotavirus enteritis in buffaloes].","authors":"L Muniiappa,&nbsp;G K Georgiev,&nbsp;Kh E Kharalambiev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunodiffusion was used to investigate a total of 492 serum samples of buffaloes--buffalo calves up to the age of 6 months and buffalo heifers--on both private and public farms. Positive serological reagents for rotaviruses were established in 73.4 per cent of the cases. The study of the same samples via the blocking ELISA test revealed the presence of 86.2 per cent positive cases. It was demonstrated that this type of the ELISA technique was a more precise method for the demonstration of group specific rotavirus antibodies in buffaloes. The results obtained spoke of the widely occurring rotavirus infections among the buffalo populations in this country. Particularly intense was the circulation of rotaviruses in buffalo calves aged up to six months. The study of 46 fecal samples from buffalo calves with diarrhea by means of the immunodiffusion and ELISA tests revealed that in as many as seven of them there were rotaviruses. This unquestionably demonstrated the participation of these agents in the etiology of enteritis in buffaloes.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 7","pages":"10-4"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13961742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Experimental infection of sheep with the virus of bovine enzootic leukemia]. 绵羊感染牛地方性白血病病毒的实验研究。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
N Sandev
{"title":"[Experimental infection of sheep with the virus of bovine enzootic leukemia].","authors":"N Sandev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intraperitoneal, the muscular, and the nasal infection with a virus, containing material (blood of a cow, affected by leukosis, with 48100 leucocytes in a cubic mm and 92% of lymphocytosis) and cell suspension FLK, producing leukosis virus, provokes leukosis infection. It was established that the peroral and the intraconjunctival application of virus material does not provoke leukosis till the 6th month (term of observation). With saliva and semen, obtained from positive serologic reagents with persistent lymphocytosis also does not provoke infection. By the immunofluorescent test, in the infected sheep were observed antibodies against the summary glycoprotein antigen, which appear after 1-2 months with continuous seroconversion till 18th month and precipitation titer 1:2 till 1:64. Among 30% of the sheep, after the 5th and 8th month, was established leucocytosis, up to 18800 leucocytes, and persistent lymphocytosis--91-93%.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 9","pages":"31-5"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13971461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Arterial vessels of the kidney in domestic swine]. [家猪肾脏的动脉血管]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
A Vodenicharov, S Danchev, I Vodenicharova
{"title":"[Arterial vessels of the kidney in domestic swine].","authors":"A Vodenicharov,&nbsp;S Danchev,&nbsp;I Vodenicharova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By histological preparation, measuring, corrosion and angiography were studied the arterial blood vessels of 120 kidneys from 60 individuals at different ages. It was established that the kidney artery in the most cases (70%) divides by the dichotomous type in cranial and caudal or in dorsal and ventral branches, and rarely (30%) by the disseminated type in 3 branches--two cranial and one caudal. Investigated were the position, course and branching of the prelobar, interlobar, the arcuate, the inter- and intra-lobular arteries.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 9","pages":"70-7"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14453669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Diagnostic value of symptoms in chick embryo pathology]. [鸡胚病理症状的诊断价值]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
M Markarian
{"title":"[Diagnostic value of symptoms in chick embryo pathology].","authors":"M Markarian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The diagnostic significance (quantitative value, weight) of the symptoms in a broad sense (pathologicoanatomic, clinical, laboratory and other data) in the diseases of the bird embryo was studied. On the basis of a complex of criteria an expert rating from 1 to 4 to 342 symptoms in 58 diseases of the bird embryo was given. The interpretation of the symptoms which are evaluated, gives an opportunity for a more precise diagnosis in comparison with the classical method, in which the symptoms are not evaluated. The quantitative exactness of the diagnostic significance according to the 4th rating scale represents an important precondition for developing algorithms, planning and computerizing of the diagnostic process in the diseases of the bird embryo.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 10","pages":"71-5"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14572671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Microbial etiology of the MMA syndrome (mastitis-metritis-agalactia) in swine raised commercially]. [商业养猪MMA综合征(乳腺炎-子宫炎-无乳症)的微生物病因学]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
N Korudzhiĭski, G Bozhkova, G V Gŭlŭbinov, I Dzhurova, S Georgiev
{"title":"[Microbial etiology of the MMA syndrome (mastitis-metritis-agalactia) in swine raised commercially].","authors":"N Korudzhiĭski,&nbsp;G Bozhkova,&nbsp;G V Gŭlŭbinov,&nbsp;I Dzhurova,&nbsp;S Georgiev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 147 uterine-cervical exudates and milk samples from 165 lobes of the mammary gland of sows with clinical symptoms of mastitis-metritis-agalactia (MMA) were studied microbiologically. It was found that greatest was the share of Escherichia coli and Staphylococcus aureus organisms taking part in the microbial etiology of the syndrome. Second ranked Staphylococcus epidermidis, Streptococcus pyogenes, and Corynebacterium pyogenes along with some aerobic sporulating bacilli.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 5","pages":"11-5"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14750687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Antibacterial activity of chlorquinaldol esters and 2-styryl-substituted derivatives]. [氯喹醛酯及其2-苯乙烯取代衍生物的抗菌活性]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
K K Kolev, S Vŭrbanova, S Chervenkov, A Pavlov
{"title":"[Antibacterial activity of chlorquinaldol esters and 2-styryl-substituted derivatives].","authors":"K K Kolev,&nbsp;S Vŭrbanova,&nbsp;S Chervenkov,&nbsp;A Pavlov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studied was the bacteriostatic activity of a total of 17 new esters and replaced 2-styryl derivatives of chlorquinaldol. Determined were also the lowest concentrations that suppressed the growth of organisms. Some of the compounds showed higher activity and broader spectrum of antibacterial qualities, mainly against Escherichia coli, Salmonella gallinarum, and Salmonella cholerae suis as compared to the initial therapeutic preparation chlorquinaldol. It was found that the presence of chlorine atoms either in the second or in the second and fourth place in the benzene nucleus of the esters studied, the presence of a NO2-group in the third place of the same nucleus, and the presence of an extranuclear hydroxyacetyl remainder in the ester grouping could lead to a rise of the antibacterial activity. The presence of an F atom in the second and third place of the benzene nucleus of the sterile grouping also raised the activity of these compounds.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 7","pages":"81-7"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14792304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Drug resistance of bacterial strains isolated from sows with the clinical picture of mastitis-metritis-agalactia]. [乳腺炎-子宫炎-无乳母猪分离菌株的耐药性研究]。
Veterinarno-meditsinski nauki Pub Date : 1987-01-01
N Korudzhiĭski, G Bozhkova, G V Gŭlŭbinov, I Dzhurova, S Georgiev
{"title":"[Drug resistance of bacterial strains isolated from sows with the clinical picture of mastitis-metritis-agalactia].","authors":"N Korudzhiĭski,&nbsp;G Bozhkova,&nbsp;G V Gŭlŭbinov,&nbsp;I Dzhurova,&nbsp;S Georgiev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The disk method with Ericson and Bauer's solid nutrient media were employed to ascertain the drug resistance of a total of 54 strains of Escherichia coli, 25 strains of Staphylococcus aureus, 11 strains of Streptococcus pyogenes, 7 strains of Corynebacterium pyogenes, 7 strains of Streptococcus uberis, 2 strains of Streptococcus agalactiae, and 1 strain of Streptococcus disgalactiae isolated from utero-cervical exudate and milk samples of sows with clinical symptoms of mastitis-metritis-agalactia (MMA). It was found that gentamycin only suppressed 100 per cent of the strains. So far as the other chemotherapeutic agents were concerned the organisms showed a varying extent of resistance. After determining the sensitivity of the strains to drugs two groups of pigs (from which the strains referred to were isolated) were subject to treatment. All 20 sows of the first group were treated with oxytocin only, while the 446 animals of the second group were treated with gentamycin in combination with oxytocin. It was found that effective therapy in the case of MMA was impossible with the application of preparations on the base of the hind lobe of the pituitary only. It is necessary to use chemotherapeutic means tow which the isolated organisms are susceptible.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 7","pages":"15-9"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14793347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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