[利用传染性腹膜炎猫腹膜液中的γ -球蛋白进行TGE抗原的免疫荧光检测]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01
G Ignatov, A Tunkara, M Ignatova
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引用次数: 0

摘要

用猫传染性腹膜炎(FIP)的腹膜渗出液分离igg -球蛋白,采用亲和层析,并将其与异硫氰酸荧光素偶联。在感染了传染性胃肠炎(TGE)病毒株Miller-3的猪的小肠横截面上测试了该结合物,同时在SPF猪的抗TGE结合物上测试了该结合物。两种缀合物均获得相同的结果,然而,抗fip缀合物的滴度高出数倍。讨论了利用抗fip偶联物进行TGE病毒抗原免疫荧光检测的可能性和优点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].

Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.

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