{"title":"[利用传染性腹膜炎猫腹膜液中的γ -球蛋白进行TGE抗原的免疫荧光检测]。","authors":"G Ignatov, A Tunkara, M Ignatova","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.</p>","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 8","pages":"44-7"},"PeriodicalIF":0.0000,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].\",\"authors\":\"G Ignatov, A Tunkara, M Ignatova\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.</p>\",\"PeriodicalId\":23492,\"journal\":{\"name\":\"Veterinarno-meditsinski nauki\",\"volume\":\"24 8\",\"pages\":\"44-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinarno-meditsinski nauki\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinarno-meditsinski nauki","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].
Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.