[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].

Abstract

Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.